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![Western Blot: BRCA1 [p Ser1524] Antibody [NB100-200] - Whole cell lysate (50 ug) from HeLa cells treated with 100 uM EPE for 4 hours (+) or mock treated (-) cells. Antibody: Affinity purified rabbit anti-Phospho BRCA1 (S1524) antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/BRCA1-[p-Ser1524]-Antibody-Western-Blot-NB100-200-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: BRCA1 [p Ser1524] Antibody [NB100-200] - Whole cell lysate (50 ug) from HeLa cells treated with 100 uM EPE for 4 hours (+) or mock treated (-) cells. Antibody: Affinity purified rabbit anti-Phospho BRCA1 (S1524) antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: BRCA1 [p Ser1524] Antibody [NB100-200] - Nuclear extract (50 ug) from HeLa cells mock irradiated or irradiated with 10 Gy of ionizing radiation. Antibody used at 0.5 ug/ml.](http://images.novusbio.com/fullsize/BRCA1-[p-Ser1524]-Antibody-Western-Blot-NB100-200-img0002.jpg)
![Proximity Ligation Assay: BRCA1 [p Ser1524] Antibody [NB100-200] - Image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).](http://images.novusbio.com/fullsize/BRCA1-[p-Ser1524]-Antibody-Proximity-Ligation-Assay-NB100-200-img0005.jpg)