| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1091AF405 | |||
| Alexa Fluor 488 | NB21-1091AF488 | |||
| Alexa Fluor 647 | NB21-1091AF647 | |||
| Alexa Fluor 700 | NB21-1091AF700 | |||
| Biotin | NB21-1091B | |||
| DyLight 405 | NB21-1091V | |||
| DyLight 488 | NB21-1091G | |||
| DyLight 550 | NB21-1091R | |||
| DyLight 650 | NB21-1091C | |||
| FITC | NB21-1091F | |||
| HRP | NB21-1091H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26018 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08511 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14043 | |||
![Dot Blot: Histone H3 [p Ser10] Antibody [NB21-1091] - Analysis of HIstone H3pS10 with picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[p-Ser10]-Antibody-Dot-Blot-NB21-1091-img0009.jpg "Dot Blot: Histone H3 [p Ser10] Antibody [NB21-1091] - Analysis of HIstone H3pS10 with picomoles of peptide.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Ser10] Antibody [NB21-1091] - IF Confocal analysis of HeLa cells using Histone H3 [p Ser10] antibody (NB21-1091, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/Histone-H3-[p-Ser10]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1091-img0017.jpg)
![Western Blot: Histone H3 [p Ser10] Antibody [NB21-1091] - Analysis of Histone H3pS10 in HeLa histone prep lysates. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Ser10]-Antibody-Western-Blot-NB21-1091-img0010.jpg)
![Immunohistochemistry-Paraffin: Histone H3 [p Ser10] Antibody [NB21-1091] - Analysis of FFPE human testis using Histone H3 [pS10] antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclei staining of Histone H3 was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Histone-H3-[p-Ser10]-Antibody-Immunohistochemistry-Paraffin-NB21-1091-img0015.jpg)
![Western Blot: Histone H3 [p Ser10] Antibody [NB21-1091] - Analysis of H3pS10 in C. elegans embryo lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Ser10]-Antibody-Western-Blot-NB21-1091-img0011.jpg)
![Western Blot: HIST1H3A [p Ser10] Antibody (921) [NBP2-43620] - Analysis of : A. 30 ug HeLa whole cell lysate/extract (untreated). B. 30 ug HeLa whole cell lysate/extract (100 ng/ml Nocodazole treatment for 24 hr) 15 % SDS-PAGE Histone H3 (phospho Ser10) antibody [921].](http://images.novusbio.com/fullsize/HIST1H3A-p-Ser10-Antibody-921-Western-Blot-NBP2-43620-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIST1H3A [p Ser10] Antibody (921) [NBP2-43620] - Analysis of : A. 30 ug HeLa whole cell lysate/extract (untreated). B. 30 ug HeLa whole cell lysate/extract (100 ng/ml Nocodazole treatment for 24 hr) 15 % SDS-PAGE Histone H3 (phospho Ser10) antibody [921].")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: HIST1H3A [p Ser10] Antibody (921) [NBP2-43620] - Analysis of Samples: HCT116 cells mock (left) and treated with 30 uM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: p21 Cip1 protein stained by p21 Cip1 antibody. Red: Histone H3S10ph (phospho Ser10) protein stained by Histone H3S10ph (phospho Ser10) antibody [921]. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/HIST1H3A-p-Ser10-Antibody-921-Immunocytochemistry-Immunofluorescence-NBP2-43620-img0003.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [p Ser10] Antibody (921) [NBP2-43620] - Mouse brain. Histone H3S10ph (phospho Ser10) stained by Histone H3S10ph (phospho Ser10) antibody [921] diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HIST1H3A-p-Ser10-Antibody-921-Immunohistochemistry-Paraffin-NBP2-43620-img0006.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [p Ser10] Antibody (921) [NBP2-43620] - Rat brain. Histone H3S10ph (phospho Ser10) stained by Histone H3S10ph (phospho Ser10) antibody [921] diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HIST1H3A-p-Ser10-Antibody-921-Immunohistochemistry-Paraffin-NBP2-43620-img0004.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [p Ser10] Antibody (921) [NBP2-43620] - Rat brain. Histone H3S10ph (phospho Ser10) antibody [921] diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HIST1H3A-p-Ser10-Antibody-921-Immunohistochemistry-Paraffin-NBP2-43620-img0005.jpg)
![Western Blot: HIST1H3A [p Ser10] Antibody [NB100-97852] - Detection of Phospho Human Histone H3 (pS10) by Western Blot. A549, HeLa, K562, and Jurkat cell lines were stimulated with 0.5 ug/ml of Calyculin A at 37 degrees C, 5% CO2 for 15 minutes. Cells were subsequently lysed and 1 ug of appropriate cell lysates were loaded into respective wells. Western blot was performed using affinity purified rabbit anti-Histone H3 (pS10) antibody NB100-97852 (20 ng/ml) and affinity purified rabbit anti-Histone H3 antibody (1 ug/ml; Western blots were developed utilizing chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/HIST1H3A-[p-Ser10]-Antibody-Western-Blot-NB100-97852-img0006.jpg "Western Blot: HIST1H3A [p Ser10] Antibody [NB100-97852] - Detection of Phospho Human Histone H3 (pS10) by Western Blot. A549, HeLa, K562, and Jurkat cell lines were stimulated with 0.5 ug/ml of Calyculin A at 37 degrees C, 5% CO2 for 15 minutes. Cells were subsequently lysed and 1 ug of appropriate cell lysates were loaded into respective wells. Western blot was performed using affinity purified rabbit anti-Histone H3 (pS10) antibody NB100-97852 (20 ng/ml) and affinity purified rabbit anti-Histone H3 antibody (1 ug/ml; Western blots were developed utilizing chemiluminescence with an exposure time of 30 seconds.")
![Immunocytochemistry/Immunofluorescence: HIST1H3A [p Ser10] Antibody [NB100-97852] - NBF-fixed asynchronous HeLa cells. Antibody used at a dilution of 1:2,500.](http://images.novusbio.com/fullsize/HIST1H3A-[p-Ser10]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-97852-img0004.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [p Ser10] Antibody [NB100-97852] - Section of human colon carcinoma (left) and mouse squamous cell carcinoma(right). Antibody: Affinity purified rabbit anti-Phospho Histone H3 (S10) used at a dilution of 1:2,500 (0.08 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/HIST1H3A-[p-Ser10]-Antibody-Immunohistochemistry-Paraffin-NB100-97852-img0003.jpg)
![Flow Cytometry: HIST1H3A [p Ser10] Antibody [NB100-97852] - Detection of phospho Histone H3 Ser10. Asynchronous Jurkat cells were fixed in 1.5% PFA and permeabilized in 90% methanol. 1 million cells were stained with 0.4 ug anti-phospho Histone H3 (S10) NB100-97852 in a 150 ul reaction.](http://images.novusbio.com/fullsize/HIST1H3A-[p-Ser10]-Antibody-Flow-Cytometry-NB100-97852-img0002.jpg)
![Western Blot: Histone H3 [p Ser10] Antibody (RM163) [NBP2-61545] - Western Blot of acid extracts of HeLa cells treated or non-treated with Nocodazole. Using NBP2-61545, showed a band of Histone H3 phosphorylated at serine 10 in HeLa cells. Observed molecular weight is ~16 kDa](http://images.novusbio.com/fullsize/Histone-H3-p-Ser10-Antibody-RM163-Western-Blot-NBP2-61545-img0003.jpg "Western Blot: Histone H3 [p Ser10] Antibody (RM163) [NBP2-61545] - Western Blot of acid extracts of HeLa cells treated or non-treated with Nocodazole. Using NBP2-61545, showed a band of Histone H3 phosphorylated at serine 10 in HeLa cells. Observed molecular weight is ~16 kDa")
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Ser10] Antibody (RM163) [NBP2-61545] - Immunocytochemistry of HeLa cells using NBP2-61545 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/Histone-H3-p-Ser10-Antibody-RM163-Immunocytochemistry-Immunofluorescence-NBP2-61545-img0002.jpg)
![Western Blot: Histone H3.1 [p Ser10] Antibody (2A5) [NBP3-26419] - Positive Western Blot detected in Hela whole cell lysate. MCF-7 whole cell lysate. PC3 whole cell lysate. All lanes: Histone H3.1 [p Ser10] Antibody at 0.5ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 15 KDa Observed band size: 15 KDa](http://images.novusbio.com/fullsize/nbp3-26419_rabbit-histone-h3-1-p-ser-10-mab-2a5-28220241152511.jpg "Western Blot: Histone H3.1 [p Ser10] Antibody (2A5) [NBP3-26419] - Positive Western Blot detected in Hela whole cell lysate. MCF-7 whole cell lysate. PC3 whole cell lysate. All lanes: Histone H3.1 [p Ser10] Antibody at 0.5ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 15 KDa Observed band size: 15 KDa")
![Immunocytochemistry/Immunofluorescence: Histone H3.1 [p Ser10] Antibody (2A5) [NBP3-26419] - Staining of Hela cells with Histone H3.1 [p Ser10] Antibody (2A5) at 1:31, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26419_rabbit-histone-h3-1-p-ser-10-mab-2a5-28220241323636.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3.1 [p Ser10] Antibody (2A5) [NBP3-26419] - Analysis of Histone H3.1 [p Ser10] Antibody (2A5) diluted at 1:100 and staining in Hela cells. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26419_rabbit-histone-h3-1-p-ser-10-mab-2a5-282202412493625.jpg)
![Immunohistochemistry-Paraffin: Histone H3 [p Ser10] Antibody (PHH3/471R) [NBP3-07398] - Formalin-fixed, paraffin-embedded human colon stained with Histone H3 Recombinant Rabbit Monoclonal Antibody (PHH3/471R).](http://images.novusbio.com/fullsize/Histone-H3-p-Ser10-Antibody-PHH3-471R-Immunohistochemistry-Paraffin-NBP3-07398-img0001.jpg "Immunohistochemistry-Paraffin: Histone H3 [p Ser10] Antibody (PHH3/471R) [NBP3-07398] - Formalin-fixed, paraffin-embedded human colon stained with Histone H3 Recombinant Rabbit Monoclonal Antibody (PHH3/471R).")
![Immunohistochemistry-Paraffin: Histone H3 [p Ser10] Antibody (PHH3/471R) [NBP3-07398] - Formalin-fixed, paraffin-embedded human liver carcinoma in colon stained with Histone H3 Recombinant Rabbit Monoclonal Antibody (PHH3/471R).](http://images.novusbio.com/fullsize/Histone-H3-p-Ser10-Antibody-PHH3-471R-Immunohistochemistry-Paraffin-NBP3-07398-img0002.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [p Ser10] Antibody (rPHH3/6824) [NBP3-13802] - Formalin-fixed, paraffin-embedded human bladder stained with HIST1H3A [p Ser10] antibody (rPHH3/6824).](http://images.novusbio.com/fullsize/HIST1H3A-p-Ser10-Antibody-rPHH3-6824-Immunohistochemistry-Paraffin-NBP3-13802-img0001.jpg "Immunohistochemistry-Paraffin: HIST1H3A [p Ser10] Antibody (rPHH3/6824) [NBP3-13802] - Formalin-fixed, paraffin-embedded human bladder stained with HIST1H3A [p Ser10] antibody (rPHH3/6824).")