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8 Results for "novus"

NBP1-05987

ZEB1 Antibody

(6 Reviews)

(92 Publications)

0.1 ml

NBP1-05987

Western Blot: ZEB1 Antibody [NBP1-05987] - Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZEB1 antibody (NBP1-05987) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-05987] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ZEB1 (NBP1-05987) used at a dilution of 1:1000 (0.2 ug/ml). Detection: DAB.

Western Blot: ZEB1 Antibody [NBP1-05987] - Analysis of ZEB1 in transfected and native mouse cell lysate. Image courtesy of anonymous customer review.

Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-05987] - Analysis of ZEB1 in human liver and spleen tissues. Image courtesy of anonymous customer review.

Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-05987] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ZEB1 (NBP1-05987) used at a dilution of 1:1000 (0.2 ug/ml). Detection: DAB.

<b>Independent Antibodies Validation. </b>Immunoprecipitation: ZEB1 Antibody [NBP1-05987] - Detection of human ZEB1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZEB1 antibody NBP1-05987 used for IP at 3 ug per reaction. ZEB1 was also immunoprecipitated by another rabbit anti-ZEB1 antibody. For blotting immunoprecipitated ZEB1, NBP1-05987 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemical staining results of EMT regulators in HG‐PanIN: SNAIL, SLUG, TWIST1, and ZEB1. We categorized and defined three grades: grade 0, 0.05. (d) IDH1R132H expression diminishes DEPTOR levels. Flag-IDH1R132H was introduced in p53−/− MEFs by retroviral transduction and whole-cell lysates immunoblotted. (e) Inhibition of endogenous mutated IDH1R132C decreases mTOR activity in a KDM4A-dependent manner. HT1080 cells were transduced with lentivirus expressing sh_KDM4A.3 or sh_Control. The cells were treated with 3 μM AGI-5198 twice every day for 5 days and whole-cell lysates blotted for markers of mTOR activation. (f) Expression of mutant IDH1 in NHA-hTERT increases mTOR signalling. IDH1WT-Flag or IDH1R132H genes were introduced in astrocytes through retroviral infection and cell lysates were blotted for mTORC2 activation. (g) IDH1R132H overexpression increases 2HG production in astrocytes. Intracellular 2HG levels were normalized to glutamate. Asterisk denotes a statistical difference between IDH1R132H and empty vector cells, two-sided t-test P<0.0001 (graph represents three independent experiments). Error bars represent standard deviation. (h) Treatment of NHA-hTERT with octyl-2HG activates mTOR signalling. The cells were treated for 8 h with 1 mM octyl-2HG. The experiment shown is a representative of triplicates. (i) Non-canonical mTOR activation in IDH1/2WT and IDH1R132H human astrocytomas. Scale bar, 50 μm. Representative samples from IDH1/2WT (n=3) and IDH1R132H (n=3) human astrocytomas are shown. Image collected and cropped by CiteAb from the following open publication (//www.nature.com/articles/ncomms12700), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Effect of restoration of macrophage A2bAR on liver.Liver was collected from WT, A2bAR KO, and CD68-Tg mice after 16 weeks of HFD as described in the methods. Relative mRNA expression was determined using the delta delta CT method with normalization to 18s rRNA. A. mRNA expression of TNF-alpha in liver. A2bAR KO (n = 5) vs WT (n = 7) p-value = 0.0271; A2bAR KO vs CD68-Tg (n = 6) p-value = 0.0210. B. mRNA expression of IL-6 in liver. A2bAR KO (n = 8) vs WT (n = 7) p-value = 0.0159; A2bAR KO vs CD68-Tg (n = 8) p-value = 0.0080. C. mRNA expression of IRS-2 in liver. CD68-Tg (n = 7) vs A2bAR KO (n = 8) p-value = 0.0300. D. Western blot analysis of liver; one representative (of 3 sets) WT, CD68-Tg and A2bAR KO group shown at 15 minutes post-insulin injection, following 16 weeks of HFD. Levels of mature SREBP-1 (68 kDa), and IRS-2 (185 kDa), were probed by Western blot analysis, using beta -actin (43 kDa) as loading control. Quantification of Western Blot results was performed with Image J software (//rsb.info.nih.gov/ij/) with normalization to beta -actin. WT to A2bAR KO: IRS-2 p-value = 0.0092, SREBP-1 p-value = 0.0154; CD68-Tg to A2bAR KO: IRS-2 p-value = 0.0247, SREBP-1 p-value = 0.0170. Data are averages ± SD. *Student two-tail t-test assuming equal variance was found significant only when p-value <0.05. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24892847), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , , , , , ,

Applications: , , , , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP1-48309

N-Cadherin Antibody (13A9)

(2 Reviews)

(41 Publications)

0.1 ml

NBP1-48309

Immunocytochemistry: N-Cadherin Antibody (13A9) [NBP1-48309] - Immunostaining of original tumor, low passage, and high passage KCI-MENG1 cells. The original patient-derived tumor (top row) showed moderate and patchy immunoreactivity for epithelial membrane antigen (EMA); strong and diffuse immunostaining for progesterone receptor (PR); and a Ki-67 proliferative index of 2-3%. There was also strong immunostaining for N-cadherin and vimentin. KCI-MENG1-LP cells (middle row) and KCI-MENG1-HP cells (bottom row) maintained expression of EMA, N-cadherin, and vimentin but had significantly reduced PR expression compared to the original tumor. Whereas Ki-67 labeling was found in only a small number of cells in the original tumor and low passage cells, it was positive in virtually all P84 cells. Scale bar 50 um. Image collected and cropped by CiteAb from the following publication (//www.translational-medicine.com/content/13/1/227), licensed under a CC-BY license.

Western Blot: N-Cadherin Antibody (13A9) [NBP1-48309] - Western blots showing a reduction in epithelial mesenchymal transition (EMT) markers upon PDK1 knockdown in Colo205 and HT29 cells using E cadherin (NBP2-19051), N cadherin (NBP1-48309) and B-actin antibody (NB600-501). The corresponding secondary antibodies used were either goat anti-rabbit IgG-HRP (NB7160) or goat anti-mouse IgG-HRP (NB7539). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33738242) licensed under a CC-BY license.

Western Blot: N-Cadherin Antibody (13A9) [NBP1-48309] - Analysis of N-Cadherin expression in HeLa whole cell lysate.

Immunocytochemistry: N-Cadherin Antibody (13A9) [NBP1-48309] - Human meningioma mouse xenograft model KCI-MENG1-LPSX generated with the spontaneously immortal cell line KCI-MENG1-LP. Tumors from immunocompromised SCID mice were dissected and the derivative cell line KCI-MENG1-LPSX CL was generated. The EMA, PR, and N-cadherin IHC of the mouse tumor highly resembled the original patient-derived tumor. The vimentin- and Ki-67-stained cells in the mouse tumor tissue were markedly more abundant and more intensely stained than in the original tumor. KCI-MENG1-LPSX CL cells displayed the same patterns of immunostaining as the high passage parent cell line KCI-MENG1-HP, including the loss of PR staining. Scale bar 50 um. Image collected and cropped by CiteAb from the following publication (//www.translational-medicine.com/content/13/1/227), licensed under a CC-BY license.

Flow (Intracellular): N-Cadherin Antibody (13A9) [NBP1-48309] - An intracellular stain was performed on HeLa with NBP1-48309 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody.

Simple Western: N-Cadherin Antibody (13A9) [NBP1-48309] - Simple Western lane view shows a specific band for N Cadherin in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

<b>Biological Strategies Validation. </b>Western Blot: N-Cadherin Antibody (13A9) [NBP1-48309] - Mitochondrial fission regulates migration and invasion in BRAF^V600E CRC cells through glucose metabolic reprograming. Western blot showing a reduction in EMT markers upon vemurafenib treatment in BRAF^V600E cells. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33738242/) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody (13A9) [NBP1-48309] - Adult mouse neural stem cells stained for N-Cadherin. Antibody at 1:100. ICC/IF image submitted by a verified customer review.

Immunohistochemistry: N-Cadherin Antibody (13A9) [NBP1-48309] - Immunohistochemical characterization of tissue cultures. Nuclei labeled with DAPI (blue). Whole-mount immunostaining of a cardiomyocyte-only spheroid 1 month in culture, N-cadherin (red) and EH-myomesin (green). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32118040/) licensed under a CC-BY license.

Flow Cytometry: N-Cadherin Antibody (13A9) [NBP1-48309] - An intracellular stain was performed on U-251 MG cells with N-Cadherin Antibody (13A9) NBP1-48309AF594 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 594.

Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody (13A9) [NBP1-48309] - HEK 293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti- at 5 ug/mL overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry: N-Cadherin Antibody (13A9) [NBP1-48309] - IHC analysis of N Cadherin in mouse liver using DAB with hematoxylin counterstain.

Immunohistochemistry-Paraffin: N-Cadherin Antibody (13A9) [NBP1-48309] - Analysis of a FFPE tissue section of human brain using 1:200 dilution of N-Cadherin antibody. The staining was developed using HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

Flow Cytometry: N-Cadherin Antibody (13A9) [NBP1-48309] - An intracellular stain was performed on HeLa cells with NBP1-48309AF488 and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Polarized RPE cell model characterization. (D) Representative images of several characteristic RPE markers: Na+ K+ ATPase, PEDF, E-cadherin, N-cadherin, claudin 19 and occludin (green); DAPI (blue); scale bar, 10 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29269042), licensed under a CC-BY licence.

Expression of hypoxia-inducible factor (HIF) proteins after bilateral renal ischemia or sham ischemia. Immunoblots for HIF-1 alpha (A–C) and HIF-2 alpha (D–F) of tissue extracts from the cortex and outer and inner medulla of the left kidneys of rats 24 h and 5 days following recovery from either sham ischemia (○) or bilateral renal ischemia (●); n = 6 per group. G: typical image of the gel following electrophoresis. H: typical image of the nitrocellulose membrane following transfer. Values are expressed as medians (25th percentile, 75th percentile). Paired comparisons were performed using the Mann-Whitney U-test. Because paired comparisons were made at two time points, P values were conservatively adjusted using the Dunn-Sidak method with k = 2. PTr, PT, and PTr*T are the outcomes of two-way analysis of variance on ranking with the factors treatment (Tr) and time (T). AU, arbitrary unit; I1, 24 h after ischemia; I5, 5 days after ischemia; S1, 24 h after sham ischemia; S5, 5 days after sham ischemia. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30110566), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-48309AF350
Alexa Fluor 405	NBP1-48309AF405
Alexa Fluor 488	NBP1-48309AF488
Alexa Fluor 532	NBP1-48309AF532
Alexa Fluor 594	NBP1-48309AF594
Alexa Fluor 647	NBP1-48309AF647
Alexa Fluor 700	NBP1-48309AF700
Alexa Fluor 750	NBP1-48309AF750
Allophycocyanin/Cy7	NBP1-48309APCCY7
Biotin	NBP1-48309B
DyLight 350	NBP1-48309UV
DyLight 405	NBP1-48309V
DyLight 488	NBP1-48309G
DyLight 550	NBP1-48309R
DyLight 594	NBP1-48309DL594
DyLight 650	NBP1-48309C
DyLight 680	NBP1-48309FR
DyLight 755	NBP1-48309IR
FITC	NBP1-48309F
HRP	NBP1-48309H
Janelia Fluor 549	NBP1-48309JF549
Janelia Fluor 646	NBP1-48309JF646
PE/Atto594	NBP1-48309PEATT594
PE/Cy5.5	NBP1-48309PECY55
PE/Cy7	NBP1-48309PECY7
mFluor Violet 450 SE	NBP1-48309MFV450
mFluor Violet 500 SE	NBP1-48309MFV500
mFluor Violet 610 SE	NBP1-48309MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-80868

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NBP1-31327

Vimentin Antibody

(3 Reviews)

(11 Publications)

0.1 ml

NBP1-31327

Knockdown Validated: Vimentin Antibody [NBP1-31327] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody.

<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NBP1-31327] - Cellular aggregates composed of equine endometrial fibroblast. Co-staining using the vimentin antibody and a cytokeratin antibody (mouse). Image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NBP1-31327] - Human primary cardiac fibroblasts stained with Vimentin (green) and smooth muscle actin (red) antibodies. Image from verified customer review.

Western Blot: Vimentin Antibody [NBP1-31327] - Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Paraffin-embedded rat ovary. Vimentin antibody at 1:500.

Western Blot: Vimentin Antibody [NBP1-31327] - 293T cell lysate: A. 20 ug. B. 10 ug. C. 5 ug. D. 1 ug.

Western Blot: Vimentin Antibody [NBP1-31327] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, membrane was blotted with Vimentin antibody at 1:50000.

Western Blot: Vimentin Antibody [NBP1-31327] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, membrane was blotted with Vimentin antibody at 1:50000.

Western Blot: Vimentin Antibody [NBP1-31327] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Vimentin Antibody [NBP1-31327] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody diluted at 1:2000.

Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NBP1-31327] - U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: Vimentin protein stained by Vimentin antibody diluted at 1:2000. Blue: Hoechst 33342 staining.

Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NBP1-31327] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Vimentin protein stained by Vimentin antibody diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Paraffin-embedded U37C xenograft, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Paraffin-embedded human lung adenocarcinoma. Vimentin antibody diluted at 1:500.

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Analysis of paraffin-embedded RT2 xenograft, using Vimentin antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Paraffin-embedded rat testis. Vimentin antibody at 1:500.

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Paraffin-embedded mouse testis. Vimentin antibody at 1:500.

Immunoprecipitation: Vimentin Antibody [NBP1-31327] - Vimentin protein from HeLa whole cell extracts using 5 ug of Vimentin antibody. Western blot analysis was performed using Vimentin antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Sandwich ELISA: Vimentin Antibody [NBP1-31327] - Detection of recombinant full-length Vimentin protein using as capture antibody at concentration of 5 ug/mL as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) (NBP2-19301) was diluted at 1:10000 and used to detect the primary antibody.

Reactivity:

Hu, Mu, Rt, Ca, Eq, Fe, Bv, Ch, Pm, Gp

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Eq, Fe, Bv, Ch, Pm, Gp

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP1-31369

Fibronectin Antibody

100 ul

NBP1-31369

Western Blot: Fibronectin Antibody [NBP1-31369] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2], N-term.

<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Fibronectin/Anastellin Antibody [NBP1-31369] - Analysis of HeLa, using NBP1-31369 at 1:200 dilution.

Immunohistochemistry-Paraffin: Fibronectin/Anastellin Antibody [NBP1-31369] - Paraffin-embedded hepatoma. Fibronectin antibody [N1N2], N-term dilution: 1:500.

Western Blot: Fibronectin/Anastellin Antibody [NBP1-31369] - Whole cell extracts (30 ug) was separated by 5 % SDS-PAGE, and blotted with Fibronectin antibody [N1N2], N-term diluted by 1:1000

Western Blot: Fibronectin/Anastellin Antibody [NBP1-31369] - Mouse tissue extracts (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2] diluted at 1:1000.

Immunoprecipitation: Fibronectin/Anastellin Antibody [NBP1-31369] - N-term immunoprecipitates Fibronectin protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 ug whole cell lysate/extract of Fibronectin protein expressing HeLa cells B : Control with 3 ug of pre-immune rabbit IgG C : Immunoprecipitation of Fibronectin by 3 ug of Fibronectin antibody [N1N2].

ELISA: Fibronectin Antibody [NBP1-31369] - Indirect ELISA analysis was performed by coating plate with 100 uL of recombinant Fibronectin protein at concentration of 10 ug/mL. The coated protein is detected with Fibronectin antibody [N1N2], N-term at rangeing from 0.5 to 140 ng/mL.

ELISA: Fibronectin Antibody [NBP1-31369] - Competitive ELISA detection of human fibronectin in plasma. Recombinant or purified native Fibronectin protein was coated to an ELISA plate at concentration of 10 ug/ml. Human plasma was applied as the competitors and was serial diluted at 1, 20, 200, 2000, 20000, 200000X dilutions. The primary antibody concentration was diluted to 14 ng/mL. Rabbit IgG antibody (HRP) was diluted at 1:2,000 and used to detect the primary antibody

Reactivity:

Hu, Mu, Rt, Bv

Species Glossary

Reactivity: Hu, Mu, Rt, Bv

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-20942

ZEB1 Antibody

(1 Publication)

0.1 ml

NBP2-20942

Western Blot: ZEB1 Antibody [NBP2-20942] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody diluted at 1:500. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed by ECL.

<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ZEB1 Antibody [NBP2-20942] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZEB1 protein stained by ZEB1 antibody [N2C1], Internal diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar = 10 um.

Chromatin Immunoprecipitation: ZEB1 Antibody [NBP2-20942] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-ZEB1 antibody. The precipitated DNA was detected by PCR with primer set targeting to LAMC2 promotor.

Western Blot: ZEB1 Antibody [NBP2-20942] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [N2C1], Internal diluted at 1:1000.

Western Blot: ZEB1 Antibody [NBP2-20942] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [N2C1], Internal diluted at 1:1000.

Western Blot: ZEB1 Antibody [NBP2-20942] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody diluted at 1:1000. The HRP conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunoprecipitation: ZEB1 Antibody [NBP2-20942] - 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of ZEB1 protein by 2 ug of ZEB1 antibody 7.5% SDS-PAGE The immunoprecipitated ZEB1 protein was detected by ZEB1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.

Reactivity:

Hu, Mu, Bv

Species Glossary

Reactivity: Hu, Mu, Bv

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-23524

Fibronectin Antibody (A17) - BSA Free

100 μg

Reactivity:

Hu, Bv, Ch

Species Glossary

Reactivity: Hu, Bv, Ch

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-23524AF350
Alexa Fluor 405	NBP2-23524AF405
Alexa Fluor 488	NBP2-23524AF488
Alexa Fluor 532	NBP2-23524AF532
Alexa Fluor 594	NBP2-23524AF594
Alexa Fluor 647	NBP2-23524AF647
Alexa Fluor 700	NBP2-23524AF700
Alexa Fluor 750	NBP2-23524AF750
Biotin	NBP2-23524B
DyLight 350	NBP2-23524UV
DyLight 405	NBP2-23524V
DyLight 488	NBP2-23524G
DyLight 550	NBP2-23524R
DyLight 594	NBP2-23524DL594
DyLight 650	NBP2-23524C
DyLight 680	NBP2-23524FR
DyLight 755	NBP2-23524IR
FITC	NBP2-23524F
HRP	NBP2-23524H
Janelia Fluor 549	NBP2-23524JF549
Janelia Fluor 646	NBP2-23524JF646
PerCP	NBP2-23524PCP
mFluor Violet 450 SE	NBP2-23524MFV450
mFluor Violet 500 SE	NBP2-23524MFV500
mFluor Violet 610 SE	NBP2-23524MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-23524-0.025mg

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NBP2-23525

Fibronectin Antibody (A32) - BSA Free

0.1ml

Reactivity:

Hu, Bv

Species Glossary

Reactivity: Hu, Bv

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP2-23524MFV610

Fibronectin Antibody (A17) [mFluor Violet 610 SE]

0.1 ml

Reactivity:

Hu, Bv, Ch

Species Glossary

Reactivity: Hu, Bv, Ch

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

mFluor Violet 610 SE

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-23524AF350
Alexa Fluor 405	NBP2-23524AF405
Alexa Fluor 488	NBP2-23524AF488
Alexa Fluor 532	NBP2-23524AF532
Alexa Fluor 594	NBP2-23524AF594
Alexa Fluor 647	NBP2-23524AF647
Alexa Fluor 700	NBP2-23524AF700
Alexa Fluor 750	NBP2-23524AF750
Biotin	NBP2-23524B
DyLight 350	NBP2-23524UV
DyLight 405	NBP2-23524V
DyLight 488	NBP2-23524G
DyLight 550	NBP2-23524R
DyLight 594	NBP2-23524DL594
DyLight 650	NBP2-23524C
DyLight 680	NBP2-23524FR
DyLight 755	NBP2-23524IR
FITC	NBP2-23524F
HRP	NBP2-23524H
Janelia Fluor 549	NBP2-23524JF549
Janelia Fluor 646	NBP2-23524JF646
PerCP	NBP2-23524PCP
mFluor Violet 450 SE	NBP2-23524MFV450
mFluor Violet 500 SE	NBP2-23524MFV500

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-23524-100ug
BSA Free	NBP2-23524-0.025mg

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