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![Western Blot: integrin beta 4 binding protein Antibody [NBP2-13954] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/integrin-beta-4-binding-protein-Antibody-Western-Blot-NBP2-13954-img0005.jpg "Western Blot: integrin beta 4 binding protein Antibody [NBP2-13954] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).")
![Immunocytochemistry/Immunofluorescence: integrin beta 4 binding protein Antibody [NBP2-13954] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/integrin-beta-4-binding-protein-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13954-img0006.jpg)
![Immunohistochemistry-Paraffin: integrin beta 4 binding protein Antibody [NBP2-13954] - Staining of human Skeletal muscle shows very weak cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/integrin-beta-4-binding-protein-Antibody-Immunohistochemistry-Paraffin-NBP2-13954-img0012.jpg)
![Western Blot: integrin beta 4 binding protein Antibody [NBP2-13954] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/integrin-beta-4-binding-protein-Antibody-Western-Blot-NBP2-13954-img0004.jpg)
![Immunohistochemistry-Paraffin: integrin beta 4 binding protein Antibody [NBP2-13954] - Staining of human Colon shows strong nuclear and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/integrin-beta-4-binding-protein-Antibody-Immunohistochemistry-Paraffin-NBP2-13954-img0010.jpg)
![Immunohistochemistry-Paraffin: integrin beta 4 binding protein Antibody [NBP2-13954] - Staining of human Liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/integrin-beta-4-binding-protein-Antibody-Immunohistochemistry-Paraffin-NBP2-13954-img0011.jpg)
![Immunohistochemistry-Paraffin: integrin beta 4 binding protein Antibody [NBP2-13954] - Staining of human Skin shows strong nuclear and cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/integrin-beta-4-binding-protein-Antibody-Immunohistochemistry-Paraffin-NBP2-13954-img0013.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: integrin beta 4 binding protein Antibody [NBP2-13954] - Analysis in human skin and skeletal muscle tissues using NBP2-13954 antibody. Corresponding EIF6 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/integrin-beta-4-binding-protein-Antibody-Immunohistochemistry-Paraffin-NBP2-13954-img0009.jpg)
![Western Blot: integrin beta 4 binding protein Antibody [NBP3-03384] - Analysis of extracts of various cell lines, using integrin beta 4 binding protein antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/integrin-beta-4-binding-protein-Antibody-Western-Blot-NBP3-03384-img0004.jpg "Western Blot: integrin beta 4 binding protein Antibody [NBP3-03384] - Analysis of extracts of various cell lines, using integrin beta 4 binding protein antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunocytochemistry/Immunofluorescence: integrin beta 4 binding protein Antibody [NBP3-03384] - Analysis of MCF-7 cells using integrin beta 4 binding protein antibody . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/integrin-beta-4-binding-protein-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03384-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: integrin beta 4 binding protein Antibody [NBP3-03384] - Analysis of Hela cells using integrin beta 4 binding protein antibody at dilution of 1:50. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/integrin-beta-4-binding-protein-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03384-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: integrin beta 4 binding protein Antibody [NBP3-03384] - Analysis of U-2OS cells using integrin beta 4 binding protein antibody at dilution of 1:50. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/integrin-beta-4-binding-protein-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03384-img0002.jpg)
![Western Blot: integrin beta 4 binding protein Antibody (HL1759) - Azide and BSA Free [NBP3-25447] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with EIF6 antibody [HL1759] (NBP3-25447) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25447_rabbit-integrin-beta-4-binding-protein-mab-hl1759-azide-bsa-free-2120249393523.jpg "Western Blot: integrin beta 4 binding protein Antibody (HL1759) - Azide and BSA Free [NBP3-25447] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with EIF6 antibody [HL1759] (NBP3-25447) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Knockdown Validated: integrin beta 4 binding protein Antibody (HL1759) - Azide and BSA Free [NBP3-25447] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with EIF6 antibody [HL1759] (NBP3-25447) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25447_rabbit-integrin-beta-4-binding-protein-mab-hl1759-azide-bsa-free-2120249415934.jpg)
![Western Blot: integrin beta 4 binding protein Antibody (HL1759) - Azide and BSA Free [NBP3-25447] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with EIF6 antibody [HL1759] (NBP3-25447) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25447_rabbit-integrin-beta-4-binding-protein-mab-hl1759-azide-bsa-free-212024938318.jpg)
![Western Blot: integrin beta 4 binding protein Antibody (HL1759) - Azide and BSA Free [NBP3-25447] - Various whole cell extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with EIF6 antibody [HL1759] (NBP3-25447) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25447_rabbit-integrin-beta-4-binding-protein-mab-hl1759-azide-bsa-free-2120249362778.jpg)
![Western Blot: integrin beta 4 binding protein Antibody [NBP1-52641] - Affinity purified rabbit anti-eIF6 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. eIF6 was also immunoprecipitated by rabbit anti-eIF6 antibody NBP1-52640, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/integrin-beta-4-binding-protein-Antibody-Western-Blot-NBP1-52641-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: integrin beta 4 binding protein Antibody [NBP1-52641] - Affinity purified rabbit anti-eIF6 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. eIF6 was also immunoprecipitated by rabbit anti-eIF6 antibody NBP1-52640, which recognizes an upstream epitope.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry: integrin beta 4 binding protein Antibody [NBP1-52641] - Samples: FFPE serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- eIF6 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/integrin-beta-4-binding-protein-Antibody-Immunohistochemistry-NBP1-52641-img0003.jpg)
![Immunohistochemistry: integrin beta 4 binding protein Antibody [NBP1-52641] - Samples: FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- eIF6 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/integrin-beta-4-binding-protein-Antibody-Immunohistochemistry-NBP1-52641-img0002.jpg)
![Western Blot: integrin beta 4 binding protein Antibody [NBP1-52640] - Affinity purified rabbit anti-eIF6 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. eIF6 was also immunoprecipitated by rabbit anti-eIF6 antibody NBP1-52641, which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/integrin-beta-4-binding-protein-Antibody-Western-Blot-NBP1-52640-img0001.jpg "<b>Independent Antibodies Validation. </b>Western Blot: integrin beta 4 binding protein Antibody [NBP1-52640] - Affinity purified rabbit anti-eIF6 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. eIF6 was also immunoprecipitated by rabbit anti-eIF6 antibody NBP1-52641, which recognizes a downstream epitope.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: integrin beta 4 binding protein Antibody [NBP1-52640] - Samples: FFPE serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- eIF6 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/integrin-beta-4-binding-protein-Antibody-Immunohistochemistry-NBP1-52640-img0003.jpg)
![Immunohistochemistry: integrin beta 4 binding protein Antibody [NBP1-52640] - Samples: FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- eIF6 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/integrin-beta-4-binding-protein-Antibody-Immunohistochemistry-NBP1-52640-img0002.jpg)
![Western Blot: integrin beta 4 binding protein Antibody [NBP2-16975] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with EIF6 antibody [N1C3-2] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/integrin-beta-4-binding-protein-Antibody-Western-Blot-NBP2-16975-img0004.jpg "Western Blot: integrin beta 4 binding protein Antibody [NBP2-16975] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with EIF6 antibody [N1C3-2] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: integrin beta 4 binding protein Antibody [NBP2-16975] - Human colon. EIF6 antibody [N1C3-2] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/integrin-beta-4-binding-protein-Antibody-Immunohistochemistry-Paraffin-NBP2-16975-img0002.jpg)
![Western Blot: integrin beta 4 binding protein Antibody [NBP3-06333] - Integrin beta 4 binding protein Antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:27 kDaObserved band size:27 kDa](http://images.novusbio.com/fullsize/integrin-beta-4-binding-protein-Antibody-Western-Blot-NBP3-06333-img0001.jpg "Western Blot: integrin beta 4 binding protein Antibody [NBP3-06333] - Integrin beta 4 binding protein Antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:27 kDaObserved band size:27 kDa")
![Immunoprecipitation: integrin beta 4 binding protein Antibody [NBP3-06333] - Integrin beta 4 binding protein Antibody was immunoprecipitated using:Lane A: 0.5 mg Hela Whole Cell Lysate4 uL anti-EIF6 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG. Primary antibody:Anti-EIF6 rabbit polyclonal antibody, at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L) HRP at 110000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 27 kDaObserved band size: 26 kDa](http://images.novusbio.com/fullsize/integrin-beta-4-binding-protein-Antibody-Immunoprecipitation-NBP3-06333-img0002.jpg)