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![Western Blot: TACE/ADAM17 Antibody [NBP1-77044] - KO Validation in HeLa Cells. Loading: 10 ug of HeLa WT cell lysates or TACE KO celllysates. Antibodies: TACE/ADAM17 NBP1-77044 (0.25 ug/mL) and beta-actin NBP1-76692 (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-Western-Blot-NBP1-77044-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: TACE/ADAM17 Antibody [NBP1-77044] - KO Validation in HeLa Cells. Loading: 10 ug of HeLa WT cell lysates or TACE KO celllysates. Antibodies: TACE/ADAM17 NBP1-77044 (0.25 ug/mL) and beta-actin NBP1-76692 (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: TACE/ADAM17 Antibody [NBP1-77044] - HeLa cells with TACE antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77044-img0004.jpg)
![Western Blot: TACE/ADAM17 Antibody [NBP1-77044] - HeLa whole cell lysate. Image from verified customer review.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-Western-Blot-NBP1-77044-img0003.jpg)
![Western Blot: TACE/ADAM17 Antibody [NBP1-77044] - Analysis of TACE in HeLa (A, D), Jurkat (B, E) and Raji (C,F) cell lysate in (A-C) the absence or (D-F) the presence of blocking peptide with TACE antibody at 0.5 ug/mL.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-Western-Blot-NBP1-77044-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: TACE/ADAM17 Antibody [NBP1-77044] - IAV via Protein Expression Profile in Cells Lines. Antibodies: TACE and GAPDH. Secondary: Goat anti-rabbit IgG HRP conjugate.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-Western-Blot-NBP1-77044-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: TACE/ADAM17 Antibody [NBP1-77044] - HeLa cells with ADAM17 antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77044-img0002.jpg)
![Western Blot: TACE/ADAM17 Antibody (JM10-35) [NBP2-67179] - Analysis of ADAM17 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SW480 cell lysateLane 2: HepG2 cell lysate](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-JM10-35-Western-Blot-NBP2-67179-img0006.jpg "Western Blot: TACE/ADAM17 Antibody (JM10-35) [NBP2-67179] - Analysis of ADAM17 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SW480 cell lysateLane 2: HepG2 cell lysate")
![Immunocytochemistry/Immunofluorescence: TACE/ADAM17 Antibody (JM10-35) [NBP2-67179] - Staining ADAM17 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-JM10-35-Immunocytochemistry-Immunofluorescence-NBP2-67179-img0005.jpg)
![Flow Cytometry: TACE/ADAM17 Antibody (JM10-35) [NBP2-67179] - Analysis of Hela cells with ADAM17 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-JM10-35-Flow-Cytometry-NBP2-67179-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: TACE/ADAM17 Antibody (JM10-35) [NBP2-67179] - Staining ADAM17 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-JM10-35-Immunocytochemistry-Immunofluorescence-NBP2-67179-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: TACE/ADAM17 Antibody (JM10-35) [NBP2-67179] - Staining ADAM17 in SKOV-3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-JM10-35-Immunocytochemistry-Immunofluorescence-NBP2-67179-img0003.jpg)
![Knockout Validated: TACE/ADAM17 Antibody [NBP2-15281] - Wild-type (WT) and ADAM17 knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [C2C3], C-term. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-Western-Blot-NBP2-15281-img0008.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: TACE/ADAM17 Antibody [NBP2-15281] - Wild-type (WT) and ADAM17 knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [C2C3], C-term. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Genetic Strategies Validation. </b>Western Blot: TACE/ADAM17 Antibody [NBP2-15281] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [C2C3], C-term diluted at 1:500.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-Western-Blot-NBP2-15281-img0005.jpg)
![Immunohistochemistry-Paraffin: TACE/ADAM17 Antibody [NBP2-15281] - Human breast carcinoma dilution: 1:250. ADAM17 antibody [C2C3], C-term dilution: 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-Immunohistochemistry-Paraffin-NBP2-15281-img0006.jpg)
![Western Blot: TACE/ADAM17 Antibody [NBP2-15281] - 30 ug K562 whole cell lysate/extract, 7.5% SDS-PAGE gel, dilution 1:500.](http://images.novusbio.com/fullsize/ADAM17-Antibody-Western-Blot-NBP2-15281-img0001.jpg)
![Western Blot: TACE/ADAM17 Antibody [NBP2-15281] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [C2C3], C-term diluted at 1:500.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-Western-Blot-NBP2-15281-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: TACE/ADAM17 Antibody [NBP1-86546] - Staining of human cell line U-2 OS shows positivity in cytoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ADAM17-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86546-img0003.jpg "Immunocytochemistry/Immunofluorescence: TACE/ADAM17 Antibody [NBP1-86546] - Staining of human cell line U-2 OS shows positivity in cytoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: TACE/ADAM17 Antibody [NBP1-86546] - Staining of human small intestine shows membranous and cytoplasmic positivity.](http://images.novusbio.com/fullsize/ADAM17-Antibody-Immunohistochemistry-Paraffin-NBP1-86546-img0002.jpg)
![Western Blot: TACE/ADAM17 Antibody [NBP2-58422] - Analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-Western-Blot-NBP2-58422-img0002.jpg "Western Blot: TACE/ADAM17 Antibody [NBP2-58422] - Analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: TACE/ADAM17 Antibody [NBP2-58422] - Staining of human cell line U-251 MG shows localization to cytosol.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58422-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: TACE/ADAM17 Antibody (5A1) [NBP3-26523] - Staining of Hela Cells with TACE/ADAM17 Antibody (5A1) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26523_rabbit-tace-adam17-mab-5a1-282202412493618.jpg "Immunocytochemistry/Immunofluorescence: TACE/ADAM17 Antibody (5A1) [NBP3-26523] - Staining of Hela Cells with TACE/ADAM17 Antibody (5A1) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).")