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NBP1-91810

CRBN Antibody

(1 Review)

(20 Publications)

0.1 ml

NBP1-91810

Western Blot: CRBN Antibody [NBP1-91810] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CRBN Antibody [NBP1-91810] - Staining of human cell line U-251 MG shows localization to nucleoli. Antibody staining is shown in green.

Western Blot: CRBN Antibody [NBP1-91810] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Simple Western: CRBN Antibody [NBP1-91810] - Simple Western lane view shows a specific band for CRBN in 0.2 mg/ml of RT4 (left), U-251MG (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: CRBN Antibody [NBP1-91810] - Electropherogram image(s) of corresponding Simple Western lane view. CRBN antibody was used at 1:60 dilution on RT4 and U-251MG lysate(s).

<b>Genetic Strategies Validation. </b>Single Cell Western: CRBN Antibody [NBP1-91810] - CRBN expression after Downregulation by siRNA, dilution 1:500. Single Cell Western Image submitted by verified customer review.

Knockdown Validated: CRBN Antibody [NBP1-91810] - Validation of SALL4 asa bona fide IMiD-dependent CRL4CRBN substrate. Parental Kelly cells or two independent pools of CRBN -/- Kelly cells were treated with increasing concentrations of thalidomide. Following 24 h incubation, SALL4, CRBN, and GAPDH protein levels were assessed by western blot analysis. Thalidomide promotes degradation of SALL4, a transcription factor implicated in Duane Radial Ray syndrome. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30067223/) licensed under a CC-BY license.

Degradation of NS3 contributes to antiviral activity. c) Degradation of NS3 contributes to the antiviral activity of the NS3 degraders. Wildtype Huh7.5 and Huh7.5 CRBN-/- cells were infected with HCV-Jc1 at a MOI of 0.1. The infected cells were treated from 24 to 48 h post-infection with a range of small molecule concentrations (indicated in nM). The intracellular abundance of HCV NS3, CRBN, and GAPDH was analyzed by Western blot. Source data are provided as a Source Data file. One representative experiment is shown from n > 3 independent experiments. NS3 abundance was normalized to the loading control (GAPDH) and is presented as a percentage of the DMSO-treated control samples. Values represent the means of n = 3 independent experiments Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31371704), licensed under a CC-BY licence.

A beta facilitates HIF1 alpha synthesis and autophagy inhibition via mTOR activation. (A) SK-N-MC cells were exposed to A beta (5 μM) for 0–48 h. HIF1 alpha and beta -actin expression was analyzed by western blot. n = 3. (B) Cells were pretreated with NAC (1 mM) for 30 min prior to A beta treatment for 24 h. HIF1 alpha and beta -actin expression were analyzed by western blot. n = 3. (C,E) Cells were incubated with rapamycin (10 nM) for 30 min prior to A beta treatment for 24 h. Phosphorylation of 4EBP1 (Thr 37/46) and 4EBP1, phosphorylation of p70S6K1 (Thr 389), HIF1 alpha and beta -actin were analyzed by western blot. n = 6. (D) Protein samples were immunoprecipitated by eukaryotic translation initiation factor 4E (eIF4E) antibody-conjugated protein A/G agarose beads. Samples were blotted with 4EBP1 and eIF4E-specific antibodies. n = 3. (F) Cells were exposed to PF4708671 (10 μM) for 30 min prior to A beta treatment for 24 h. HIF1 alpha and beta -actin expression was detected by western blot. n = 6. (G) Cells were exposed to cycloheximide (4 μM) for 30 min prior to A beta treatment for 24 h. HIF1 alpha and beta -actin expressions were detected by western blot. n = 6. (H) Cells were pretreated with rapamycin (10 nM) for 30 min, incubated with A beta for 24 h and analyzed by western blotting with LC3, p62 and beta -actin specific antibodies. n = 3–6. (I) LC3 puncta was visualized by confocal microscopy. Presented results are merged images. Green and red fluorescents indicate LC3 and PI respectively. Scale bars, 50 μm (magnification × 600). (J) Cells were pretreated with trehalose (10 μM) for 30 min prior to A beta treatment for 24 h. Cytotoxicity was measured by MTT assay at an absorbance of 545 nm using a microplate reader. Data present the mean ± SE. n = 6. (K) Cell viability was measured by trypan blue exclusion assay. Data are presented as a mean ± SE. n = 6. Each blot image was presented as representative image. *p < 0.05 vs. control, #p < 0.05 vs. A beta treatment. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/28790888), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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MAB11226

VSIG3/IGSF11 Antibody (2637B) [Unconjugated]

100 μg

MAB11226

Simple Western lane view shows lysates of IMR‑32 human neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for VSIG3 at approximately 57 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human VSIG3 Monoclonal Antibody (Catalog # MAB11226) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western blot shows lysates of IMR‑32 human neuroblastoma cell line, SH‑SY5Y human neuroblastoma cell line and Mouse cerebellum. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human VSIG3 Monoclonal Antibody (Catalog # MAB11226) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for VSIG3 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB11226-SP

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