Note: Mouseover a species abbreviation on the product page to display the fullname.
How can we help you?
![Western Blot: Caspase-8 Antibody (90A992) [NB100-56527] - Western blot analysis of Caspase-8 in Jurkat cells using Caspase-8 antibody at 1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. Caspase-8 activation is detected in western blots by the presence of Caspase-8 cleavage fragments. The antibody detected both pro (full length) and active (cleaved) Caspase-8, depending on the treatment time points. A basal level of endogenously cleaved Caspase-8 can be see in untreated Jurkat cells. Goat anti-mouse lg HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-90A992-Western-Blot-NB100-56527-img0018.jpg "<b>Biological Strategies Validation. </b>Western Blot: Caspase-8 Antibody (90A992) [NB100-56527] - Western blot analysis of Caspase-8 in Jurkat cells using Caspase-8 antibody at 1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. Caspase-8 activation is detected in western blots by the presence of Caspase-8 cleavage fragments. The antibody detected both pro (full length) and active (cleaved) Caspase-8, depending on the treatment time points. A basal level of endogenously cleaved Caspase-8 can be see in untreated Jurkat cells. Goat anti-mouse lg HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![<b>Biological Strategies Validation. </b>Western Blot: Caspase-8 Antibody (90A992) [NB100-56527] - Caspase-8 expression in 786-O and A-498 cellsClose](http://images.novusbio.com/fullsize/Caspase-8-Antibody-90A992-Western-Blot-NB100-56527-img0022.jpg)
![Immunohistochemistry-Paraffin: Caspase-8 Antibody (90A992) [NB100-56527] - Formalin-fixed, paraffin-embedded human spleen (top) and breast (bottom) stained with Caspase-8 antibody at 4 ug/ml. Localization can be cytoplasmic and nuclear. Cancer/normal adjacent tissue array was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-90A992-Immunohistochemistry-Paraffin-NB100-56527-img0014.jpg)
![Flow Cytometry: Caspase-8 Antibody (90A992) [NB100-56527] - Flow cytometric analysis of Caspase-8 in HeLa cells using 0.1 ug of Caspase-8 antibody. Shaded histogram represents cells without antibody; green represents isotype control; red represents Caspase-8 antibody. Goat anti-mouse IgG-FITC secondary antibody was used for this test. IC-Flow (Intracellular Staining Flow Cytometry Kit) was used to fix and prepare the cells for staining.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-90A992-Flow-Cytometry-NB100-56527-img0008.jpg)
![Western Blot: Caspase-8 Antibody (90A992) [NB100-56527] - Analysis using the Biotin conjugate of NB100-56527. Detection of human Caspase-8 using Jurkat lysates with NB100-55786 at 2 ug/ml (lane 1) and 0.5 ug/ml (lane 2) dilution. NB100-55786 only detects 55 kDa Caspase-8 in Jurkat cells.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-90A992-Western-Blot-NB100-56527-img0021.jpg)
![Simple Western: Caspase-8 Antibody (90A992) [NB100-56527] - Simple Western lane view shows a specific band for Caspase 8 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-90A992-Simple-Western-NB100-56527-img0019.jpg)
![Western Blot: Caspase-8 Antibody (1H11) [NBP2-22183] - Analysis using Caspase 8 mouse mAb against Hela (1), Jurkat (2), THP-1 (3), NIH/3T3 (4), Cos7 (5) and PC-12 (6) cell lysate.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-1H11-Western-Blot-NBP2-22183-img0005.jpg "Western Blot: Caspase-8 Antibody (1H11) [NBP2-22183] - Analysis using Caspase 8 mouse mAb against Hela (1), Jurkat (2), THP-1 (3), NIH/3T3 (4), Cos7 (5) and PC-12 (6) cell lysate.")
![Immunohistochemistry-Paraffin: Caspase-8 Antibody (1H11) [NBP2-22183] - Analysis of liver cancer tissues using Caspase 8 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-1H11-Immunohistochemistry-Paraffin-NBP2-22183-img0003.jpg)
![Flow Cytometry: Caspase-8 Antibody (1H11) [NBP2-22183] - Analysis of NIH/3T3 cells using Caspase 8 mouse mAb (blue) and negative control (red).](http://images.novusbio.com/fullsize/Caspase-8-Antibody-1H11-Flow-Cytometry-NBP2-22183-img0001.jpg)
![Immunohistochemistry-Paraffin: Caspase-8 Antibody (1H11) [NBP2-22183] - Analysis of colon cancer tissues using Caspase 8 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-1H11-Immunohistochemistry-Paraffin-NBP2-22183-img0002.jpg)
![ELISA: Caspase-8 Antibody (1H11) [NBP2-22183] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/Caspase-8-Antibody-1H11-ELISA-NBP2-22183-img0004.jpg)
![Western Blot: Caspase-8 Antibody (1G12) - BSA Free [NBP2-80097] - Using Caspase-8 (mouse), mAb (1G12) (Prod. No. NBP2-80097) detecting the cleaved active p20 subunit of mouse caspase-8 in addition to the caspase-8 precursor, upon an apoptotic stimulus e.g. by cross-linked rhsFasL.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-1G12-BSA-Free-Western-Blot-NBP2-80097-img0004.jpg "Western Blot: Caspase-8 Antibody (1G12) - BSA Free [NBP2-80097] - Using Caspase-8 (mouse), mAb (1G12) (Prod. No. NBP2-80097) detecting the cleaved active p20 subunit of mouse caspase-8 in addition to the caspase-8 precursor, upon an apoptotic stimulus e.g. by cross-linked rhsFasL.")
![Flow Cytometry: Caspase-8 Antibody (1G12) - BSA Free [NBP2-80097] - Data in permeabilized cells of overexpressed FLAG-epitope-tagged pro-caspase-8 (active-site-mutant) in 293T cells using Caspase-8 mAbs (1G12) (Prod. No. NBP2-80097)](http://images.novusbio.com/fullsize/Caspase-8-Antibody-1G12-BSA-Free-Flow-Cytometry-NBP2-80097-img0001.jpg)
![Western Blot: Caspase-8 Antibody (1G12) - BSA Free [NBP2-80097] - Using Caspase-8 (mouse), mAb (1G12) (Prod. No. NBP2-80097) detecting endogenous caspase-8 in various mouse cell line, but not in human cell lines.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-1G12-BSA-Free-Western-Blot-NBP2-80097-img0002.jpg)
![Western Blot: Caspase-8 Antibody (1G12) - BSA Free [NBP2-80097] - Using Caspase-8 (mouse), mAb (1G12) (Prod. No. NBP2-80097) detecting endogenous caspase-8 in MEFs from WT mice, but not in MEFs from caspase-8-/- mice.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-1G12-BSA-Free-Western-Blot-NBP2-80097-img0003.jpg)
![Western Blot: Caspase-8 Antibody (3B10) - BSA Free [NBP2-80098] - Using Caspase-8 (mouse), mAb (3B10) (Prod. No. NBP2-80098) detecting endogenous caspase-8 in MEFs from WT mice, but not in MEFs from caspase-8-/- mice.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-3B10-BSA-Free-Western-Blot-NBP2-80098-img0004.jpg "Western Blot: Caspase-8 Antibody (3B10) - BSA Free [NBP2-80098] - Using Caspase-8 (mouse), mAb (3B10) (Prod. No. NBP2-80098) detecting endogenous caspase-8 in MEFs from WT mice, but not in MEFs from caspase-8-/- mice.")
![Flow Cytometry: Caspase-8 Antibody (3B10) - BSA Free [NBP2-80098] - Data in permeabilized cells of overexpressed FLAG-epitope-tagged pro-caspase-8 (active-site-mutant) in 293T cells using Caspase-8 mAbs (3B10) (Prod. No. NBP2-80098)](http://images.novusbio.com/fullsize/Caspase-8-Antibody-3B10-BSA-Free-Flow-Cytometry-NBP2-80098-img0001.jpg)
![Western Blot: Caspase-8 Antibody (3B10) - BSA Free [NBP2-80098] - Using Caspase-8 (mouse), mAb (3B10) (Prod. No. NBP2-80098) detecting endogenous caspase-8 in various mouse cell line, but not in human cell lines.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-3B10-BSA-Free-Western-Blot-NBP2-80098-img0002.jpg)
![Western Blot: Caspase-8 Antibody (3B10) - BSA Free [NBP2-80098] - Using Caspase-8 (mouse), mAb (3B10) (Prod. No. NBP2-80098) detecting the cleaved active p20 subunit of mouse caspase-8 in addition to the caspase-8 precursor, upon an apoptotic stimulus e.g. by cross-linked rhsFasL.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-3B10-BSA-Free-Western-Blot-NBP2-80098-img0003.jpg)