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![Western Blot: CD133 Antibody - BSA Free [NB120-16518] - Predicted band size : 97 kDa recognizes 97 kDa human CD133 in Y79 cells. Band is at ~120 kDa due to protein glycosylation.](http://images.novusbio.com/fullsize/CD133-Antibody---BSA-Free-Western-Blot-NB120-16518-img0003.jpg "Western Blot: CD133 Antibody - BSA Free [NB120-16518] - Predicted band size : 97 kDa recognizes 97 kDa human CD133 in Y79 cells. Band is at ~120 kDa due to protein glycosylation.")
![Immunocytochemistry/Immunofluorescence: CD133 Antibody - BSA Free [NB120-16518] - Characterization of Scattered tubular-like cells (STCs) isolated from pig kidneys. A) Representative immunofluorescence staining (original magnification 40X) for the STC surface markers CD133 NB120-16518G (red) and CD24 (green) in isolated swine STCs. B) Flow cytometry analysis of isolated STCs showing that 97% of cells expressed CD133, 95% CD24, and 98% were double-positive for CD133 and CD24 (n = 3 each). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31614781/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD133 Antibody - BSA Free-Immunocytochemistry-Immunofluorescence-NB120-16518-img0008.jpg)
![Immunohistochemistry: CD133 Antibody - BSA Free [NB120-16518] - Immunostaining for CD133 (NB120-16518) in normal ovarian and tumor tissue sections. Staining of CD133 in OSE layer (A, B) as well as cortex (C) reveals specific CD133+ cells with relatively higher cell numbers in BL and HG. Area within dotted lines in BN OSE (A) are magnified in (B) while elliptical/spindle shaped CD133+ cells in cortex from various fields were represented in the composite image in (C) of BN and HG. Large CD133+ cells in cortex were also observed. White scale bar?=?50 um; blue scale bar?=?10 um. Secondary antibody employed was conjugated with Alexa fluor 568 and tissue sections were counterstained with nucleus specific dye DAPI. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30121075/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD133-Antibody---BSA-Free-Immunohistochemistry-NB120-16518-img0007.jpg)
![Flow Cytometry: CD133 Antibody - BSA Free [NB120-16518] - Flow Cytometry: CD133 Antibody [DyLight 488] [NB120-16518G] - Flow Cytometry: CD133 Antibody (DyLight488) [NB120-16518G] - Rat bone marrow cells were stained with CD133 (1:100) antibody (20 minutes at 4C), fixed, and then analyzed. Image using the DyLight 488 form of this antibody.](http://images.novusbio.com/fullsize/CD133-Antibody---BSA-Free-Flow-Cytometry-NB120-16518-img0006.jpg)
![Chromatin Immunoprecipitation: CD133 Antibody - BSA Free [NB120-16518] - [NB 120-16518] - Staining CD133 in Bone Marrow Mononuclear Cells by Immunofluorescence.](http://images.novusbio.com/fullsize/CD133-Antibody---BSA-Free-Chromatin-Immunoprecipitation-NB120-16518-img0005.jpg)
![Immunohistochemistry-Paraffin: CD133 Antibody - BSA Free [NB120-16518] - Staining of CD133 in human hepatocarcinoma (HC) tissue. NB120-16518 was peroxidase-conjugated to the secondary antibody, followed by AEC staining.](http://images.novusbio.com/fullsize/CD133-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB120-16518-img0002.jpg)
![Immunostaining for CD133 in normal ovarian and tumor tissue sections: [A] Mouse monoclonal anti-CD133 antibody was localized in both OSE (A, B) and ovarian cortex (C, D) by immunohistochemistry. Region between dotted boxes in A, C is magnified in B, D respectively. Polar staining of CD133 is obvious in OSE layer especially in NO, BL and HG ovaries. BL ovaries exhibit multi-layered OSE. Cortex comprised of CD133+ cells arranged in clusters with elongated/spindle shaped morphology in NO and BN ovaries. BL ovarian cortex harbours single spherical cell clusters distributed throughout. HG comprised more of large CD133+ cells in OSE and few clusters in the cortex per field focussed. Insets include magnified images of cells from different fields. Scale bar = 100 um (A, C) and 25 um (B, D) respectively. [B] Immunofluorescence staining of CD133 in OSE layer (A, B) as well as cortex (C) reveals specific CD133+ cells with relatively higher cell numbers in BL and HG. Area within dotted lines in BN OSE (A) are magnified in (B) while elliptical/spindle shaped CD133+ cells in cortex from various fields were represented in the composite image in (C) of BN and HG. Large CD133+ cells in cortex were also observed. White scale bar = 50 um; blue scale bar = 10 um. Secondary antibody employed was conjugated with Alexa fluor 568 and tissue sections were counterstained with nucleus specific dye DAPI Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30121075), licensed under a CC-BY licence.](http://images.novusbio.com/fullsize/nb120-16518_rabbit-polyclonal-cd133-antibody-291220231211832.jpg)