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![Western Blot: Pax6 Antibody [NBP2-19711] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NBP2-19711-img0023.jpg "Western Blot: Pax6 Antibody [NBP2-19711] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Pax6 Antibody [NBP2-19711] - SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PAX6 protein stained by PAX6 antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19711-img0013.jpg)
![Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] - Mouse retina. Green: PAX6 protein stained by PAX6 antibody diluted at 1:250. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [1338] diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunohistochemistry-Paraffin-NBP2-19711-img0021.jpg)
![Western Blot: Pax6 Antibody [NBP2-19711] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody diluted by 1:1000.](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NBP2-19711-img0011.jpg)
![Western Blot: Pax6 Antibody [NBP2-19711] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NBP2-19711-img0015.jpg)
![Western Blot: Pax6 Antibody [NBP2-19711] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NBP2-19711-img0017.jpg)
![Western Blot: Pax6 Antibody [NBP2-19711] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NBP2-19711-img0019.jpg)
![Immunohistochemistry-Frozen: Pax6 Antibody [NBP2-19711] - Frozen sectioned E13.5 Rat brain. Green: PAX6 protein stained by PAX6 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunohistochemistry-Frozen-NBP2-19711-img0010.jpg)
![Immunohistochemistry-Frozen: Pax6 Antibody [NBP2-19711] - Frozen section of embryonic mouse brain (mE12.5). PAX6 antibody diluted at 1:250.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunohistochemistry-Frozen-NBP2-19711-img0012.jpg)
![Immunoprecipitation: PAX6 Antibody [NBP2-19711] - Sample: 1000 ug 293T whole cell lysate/extract A. 50 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of PAX6 protein by 2 ug of PAX6 antibody 10% SDS-PAGE The immunoprecipitated PAX6 protein was detected by PAX6 antibody diluted at 1:1000. anti-rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/PAX6-Antibody-Immunoprecipitation-NBP2-19711-img0004.jpg)
![Western Blot: Pax6 Antibody (PAX6/498) - Azide and BSA Free [NBP2-34705] - analysis of PAX6 in human cerebellum lysate](http://images.novusbio.com/fullsize/Pax6-Antibody-PAX6-498-Azide-and-BSA-Free-Western-Blot-NBP2-34705-img0001.jpg "Western Blot: Pax6 Antibody (PAX6/498) - Azide and BSA Free [NBP2-34705] - analysis of PAX6 in human cerebellum lysate")
![Immunocytochemistry/Immunofluorescence: Pax6 Antibody [NBP1-89100] - Staining of human cell line HEK 293 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89100-img0007.jpg "Immunocytochemistry/Immunofluorescence: Pax6 Antibody [NBP1-89100] - Staining of human cell line HEK 293 shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Pax6 Antibody [NBP1-89100] - Staining of human endometrium shows no positivity as expected.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunohistochemistry-Paraffin-NBP1-89100-img0012.jpg)
![Immunohistochemistry-Paraffin: Pax6 Antibody [NBP1-89100] - Staining of human pancreas shows strong nuclear positivity in islet cells.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunohistochemistry-Paraffin-NBP1-89100-img0005.jpg)
![Immunohistochemistry-Paraffin: Pax6 Antibody [NBP1-89100] - Staining of human cerebellum shows moderate to strong nuclear positivity in cells in granular layer](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunohistochemistry-Paraffin-NBP1-89100-img0009.jpg)
![Immunohistochemistry-Paraffin: Pax6 Antibody [NBP1-89100] - Staining of human stomach shows strong nuclear positivity in a subset glandular cells.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunohistochemistry-Paraffin-NBP1-89100-img0011.jpg)
![SDS-Page: Recombinant Human Pax6 Protein [H00005080-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00005080-P01-1.jpg "SDS-Page: Recombinant Human Pax6 Protein [H00005080-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: Pax6 Antibody [NB100-61654] - (0.5ug/ml) staining of RAW264.7 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NB100-61654-img0002.jpg "Western Blot: Pax6 Antibody [NB100-61654] - (0.5ug/ml) staining of RAW264.7 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Flow Cytometry: Pax6 Antibody [NB100-61654] - Flow cytometric analysis of paraformaldehyde fixed U251 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Pax6-Antibody-Flow-Cytometry-NB100-61654-img0003.jpg)
![Western Blot: Pax6 Antibody [NB100-61654] - (0.3ug/ml) staining of U251 (A), (0.03ug/ml) nuclear K562 (B) and (1ug/ml) nuclear HeLa (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NB100-61654-img0001.jpg)
![Western Blot: Pax6 Antibody (GT9412) [NBP3-13636] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Pax6 antibody [GT9412] (NBP3-13636) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/Pax6-Antibody-GT9412-Western-Blot-NBP3-13636-img0003.jpg "Western Blot: Pax6 Antibody (GT9412) [NBP3-13636] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Pax6 antibody [GT9412] (NBP3-13636) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![Immunohistochemistry-Paraffin: Pax6 Antibody (GT9412) [NBP3-13636] - Pax6 antibody [GT9412] detects Pax6 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. Green: Pax6 stained by Pax6 antibody [GT9412] (NBP3-13636) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:250. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Pax6-Antibody-GT9412-Immunohistochemistry-Paraffin-NBP3-13636-img0002.jpg)
![Immunohistochemistry-Paraffin: Pax6 Antibody (GT9412) [NBP3-13636] - Pax6 antibody [GT9412] detects Pax6 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. Pax6 stained by Pax6 antibody [GT9412] (NBP3-13636) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Pax6-Antibody-GT9412-Immunohistochemistry-Paraffin-NBP3-13636-img0001.jpg)
![Immunohistochemistry-Paraffin: Pax6 Antibody (PAX6/7078R) [NBP3-13988] - Formalin-fixed, paraffin-embedded human pancreas stained with Pax6 antibody (PAX6/7078R). Inset: PBS instead of primary antibody; secondary only negative control.](http://images.novusbio.com/fullsize/Pax6-Antibody-PAX6-7078R-Immunohistochemistry-Paraffin-NBP3-13988-img0001.jpg "Immunohistochemistry-Paraffin: Pax6 Antibody (PAX6/7078R) [NBP3-13988] - Formalin-fixed, paraffin-embedded human pancreas stained with Pax6 antibody (PAX6/7078R). Inset: PBS instead of primary antibody; secondary only negative control.")
![Immunohistochemistry: Pax6 Antibody (22C3) [NBP3-26437] - Image of Pax6 Antibody (22C3) diluted at 1:100 and staining in paraffin-embedded human pancreati tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.55% DAB.](http://images.novusbio.com/fullsize/nbp3-26437_rabbit-pax6-mab-22c3-262202414225221.jpg "Immunohistochemistry: Pax6 Antibody (22C3) [NBP3-26437] - Image of Pax6 Antibody (22C3) diluted at 1:100 and staining in paraffin-embedded human pancreati tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.55% DAB.")
![Immunohistochemistry: Pax6 Antibody (22C3) [NBP3-26437] - Image of Pax6 Antibody (22C3) diluted at 1:100 and staining in paraffin-embedded human pancreati tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.55% DAB.](http://images.novusbio.com/fullsize/nbp3-26437_rabbit-pax6-mab-22c3-262202415475614.jpg)
![Flow Cytometry: Pax6 Antibody (22C3) [NBP3-26437] - Overlay Peak curve showing SH-SY5Y cells stained with Pax6 Antibody (22C3) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26437_rabbit-pax6-mab-22c3-282202414312722.jpg)
![Western Blot: Pax6 Antibody (S03-8G4) [NBP3-19729] - Western blot detection of Pax6 in Rat Brain lysates using NBP3-19729 (1:1000 diluted). Predicted band size: 47kDa. Observed band size: 47kDa.](http://images.novusbio.com/fullsize/Pax6-Antibody-S03-8G4-Western-Blot-NBP3-19729-img0001.jpg "Western Blot: Pax6 Antibody (S03-8G4) [NBP3-19729] - Western blot detection of Pax6 in Rat Brain lysates using NBP3-19729 (1:1000 diluted). Predicted band size: 47kDa. Observed band size: 47kDa.")
![Western Blot: Pax6 Antibody (S01-1G8) [NBP3-19528] - Western blot detection of Pax6 in K562, Hela cell lysates using NBP3-19528 (1:1000 diluted). Predicted band size: 47kDa. Observed band size: 47kDa.](http://images.novusbio.com/fullsize/Pax6-Antibody-S01-1G8-Western-Blot-NBP3-19528-img0001.jpg "Western Blot: Pax6 Antibody (S01-1G8) [NBP3-19528] - Western blot detection of Pax6 in K562, Hela cell lysates using NBP3-19528 (1:1000 diluted). Predicted band size: 47kDa. Observed band size: 47kDa.")