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![Western Blot: USP7 Antibody [NB100-513] - Samples: Wholecell lysate (10 ug) from HEK293T, RKO, Hep-G2, Jurkat,HeLa, TCMK-1, and NIH 3T3 cells prepared using NETNlysis buffer. Antibody: Affinity purified rabbit anti-USP7antibody used for WB at0.04 ug/ml. Detection: Chemiluminescence with anexposure time of 10 seconds](http://images.novusbio.com/fullsize/USP7-Antibody-Western-Blot-NB100-513-img0004.jpg "Western Blot: USP7 Antibody [NB100-513] - Samples: Wholecell lysate (10 ug) from HEK293T, RKO, Hep-G2, Jurkat,HeLa, TCMK-1, and NIH 3T3 cells prepared using NETNlysis buffer. Antibody: Affinity purified rabbit anti-USP7antibody used for WB at0.04 ug/ml. Detection: Chemiluminescence with anexposure time of 10 seconds")
![Immunohistochemistry-Paraffin: USP7 Antibody [NB100-513] - Sample: FFPE section of human ovarian carcinoma.Antibody: Affinity purified rabbit anti-USP7 used at 1:5,000 (0.2ug/ml). Detection:DAB](http://images.novusbio.com/fullsize/USP7-Antibody-Immunohistochemistry-Paraffin-NB100-513-img0002.jpg)
![Immunohistochemistry-Paraffin: USP7 Antibody [NB100-513] - Sample: FFPE section of mouse renal cell carcinoma.Antibody: Affinity purified rabbit anti-USP7 used at 1:5,000 (0.2ug/ml). Detection:DAB](http://images.novusbio.com/fullsize/USP7-Antibody-Immunohistochemistry-Paraffin-NB100-513-img0001.jpg)
![Immunoprecipitation: USP7 Antibody [NB100-513] - Samples: Wholecell lysate (10 ug) from HEK293T, RKO, Hep-G2, Jurkat,HeLa, TCMK-1, and NIH 3T3 cells prepared using NETNlysis buffer. Antibody: Affinity purified rabbit anti-USP7antibody used for WB at0.04 ug/ml. Detection: Chemiluminescence with anexposure time of 10 seconds](http://images.novusbio.com/fullsize/USP7-Antibody-Immunoprecipitation-NB100-513-img0003.jpg)
![Western Blot: USP7 Antibody [NBP2-24641] - analysis of USP7 in MCF7 cell lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. 0 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/USP7-Antibody-Western-Blot-NBP2-24641-img0002.jpg "Western Blot: USP7 Antibody [NBP2-24641] - analysis of USP7 in MCF7 cell lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. 0 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: USP7 Antibody [NBP2-24641] - analysis of USP7 in formalin-fixed, paraffin-embedded human stomach tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.](http://images.novusbio.com/fullsize/USP7-Antibody-Immunohistochemistry-Paraffin-NBP2-24641-img0001.jpg)
![Western Blot: USP7 Antibody (1F12) [NBP2-01544] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP7.](http://images.novusbio.com/fullsize/USP7-Antibody-1F12-Western-Blot-NBP2-01544-img0002.jpg "Western Blot: USP7 Antibody (1F12) [NBP2-01544] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP7.")
![Immunohistochemistry-Paraffin: USP7 Antibody (1F12) [NBP2-01544] - Staining of paraffin-embedded Human prostate tissue using anti-USP7 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/USP7-Antibody-1F12-Immunohistochemistry-Paraffin-NBP2-01544-img0012.jpg)
![Flow Cytometry: USP7 Antibody (1F12) [NBP2-01544] - Analysis of Hela cells, using anti-USP7 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/USP7-Antibody-1F12-Flow-Cytometry-NBP2-01544-img0001.jpg)
![Immunohistochemistry-Paraffin: USP7 Antibody (1F12) [NBP2-01544] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-USP7 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/USP7-Antibody-1F12-Immunohistochemistry-Paraffin-NBP2-01544-img0004.jpg)
![Immunohistochemistry-Paraffin: USP7 Antibody (1F12) [NBP2-01544] - Staining of paraffin-embedded Human colon tissue using anti-USP7 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/USP7-Antibody-1F12-Immunohistochemistry-Paraffin-NBP2-01544-img0008.jpg)
![Immunohistochemistry-Paraffin: USP7 Antibody (1F12) [NBP2-01544] - Staining of paraffin-embedded Human Kidney tissue using anti-USP7 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/USP7-Antibody-1F12-Immunohistochemistry-Paraffin-NBP2-01544-img0010.jpg)
![Immunohistochemistry-Paraffin: USP7 Antibody (1F12) [NBP2-01544] - Staining of paraffin-embedded Human pancreas tissue using anti-USP7 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/USP7-Antibody-1F12-Immunohistochemistry-Paraffin-NBP2-01544-img0011.jpg)
![USP7 Antibody (BLR072G) [NBP2-78709] - Detection of human USP7/HAUSP in FFPE prostate carcinoma by IHC using this antibody. Secondary: HRP-conjugated goat anti-rabbit IgG (NB100-2332). Substrate: DAB.](http://images.novusbio.com/fullsize/USP7-Antibody-BLR072G-NBP2-78709-img0001.jpg "USP7 Antibody (BLR072G) [NBP2-78709] - Detection of human USP7/HAUSP in FFPE prostate carcinoma by IHC using this antibody. Secondary: HRP-conjugated goat anti-rabbit IgG (NB100-2332). Substrate: DAB.")
![USP7 Antibody (BLR072G) [NBP2-78709] - Detection of mouse USP7/HAUSP in FFPE renal cell carcinoma by IHC using this antibody. Secondary: HRP-conjugated goat anti-rabbit IgG (NB100-2332). Substrate: DAB.](http://images.novusbio.com/fullsize/USP7-Antibody-BLR072G-NBP2-78709-img0002.jpg)
![USP7 Antibody (BLR072G) [NBP2-78709] - Detection of human USP7/HAUSP in FFPE OVCAR-4 cells by ICC using this antibody. Secondary: HRP-conjugated goat anti-rabbit IgG (NB100-2332). Substrate: DAB.](http://images.novusbio.com/fullsize/USP7-Antibody-BLR072G-NBP2-78709-img0003.jpg)
![USP7 Antibody (BLR072G) [NBP2-78709] - Detection of human and mouse USP7/HAUSP by WB of RKO, HEK293T, K-562, Hep-G2, MCF-7, Jurkat, HeLa, U2OS, NIH 3T3, and TCMK-1 lysate using this antibody. Secondary: HRP-conjugated goat anti-rabbit IgG (NB7160). Lower Panel: Rabbit anti-eEF2 (NB100-87020).](http://images.novusbio.com/fullsize/USP7-Antibody-BLR072G-NBP2-78709-img0004.jpg)
![USP7 Antibody (BLR072G) [NBP2-78709] - Detection of human USP7/HAUSP by WB of immunoprecipitates from HEK293T lysate using this antibody and rabbit anti-USP7 (NB100-2296).](http://images.novusbio.com/fullsize/USP7-Antibody-BLR072G-NBP2-78709-img0005.jpg)
![Western Blot: USP7 Antibody [NBP2-24642] - analysis of USP7 protein in MCF7 cell lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. 0 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/USP7-Antibody-Western-Blot-NBP2-24642-img0001.jpg "Western Blot: USP7 Antibody [NBP2-24642] - analysis of USP7 protein in MCF7 cell lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. 0 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Western Blot: USP7 Antibody (3J7G1) [NBP3-16188] - Western blot analysis of extracts of various cell lines, using USP7 Rabbit mAb (NBP3-16188) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/USP7-Antibody-3J7G1-Western-Blot-NBP3-16188-img0004.jpg "Western Blot: USP7 Antibody (3J7G1) [NBP3-16188] - Western blot analysis of extracts of various cell lines, using USP7 Rabbit mAb (NBP3-16188) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: USP7 Antibody (3J7G1) [NBP3-16188] - Immunohistochemistry of paraffin-embedded mouse brain using USP7 Rabbit mAb (NBP3-16188) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/USP7-Antibody-3J7G1-Immunohistochemistry-Paraffin-NBP3-16188-img0003.jpg)
![Immunohistochemistry-Paraffin: USP7 Antibody (3J7G1) [NBP3-16188] - Immunohistochemistry of paraffin-embedded rat brain using USP7 Rabbit mAb (NBP3-16188) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/USP7-Antibody-3J7G1-Immunohistochemistry-Paraffin-NBP3-16188-img0001.jpg)
![Immunohistochemistry-Paraffin: USP7 Antibody (3J7G1) [NBP3-16188] - Immunohistochemistry of paraffin-embedded human colon carcinoma using USP7 Rabbit mAb (NBP3-16188) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/USP7-Antibody-3J7G1-Immunohistochemistry-Paraffin-NBP3-16188-img0002.jpg)
![Immunohistochemistry: USP7 Antibody (7F10) [NBP3-26258] - Image of USP7 Antibody (7F10) diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26258_rabbit-usp7-mab-7f10-262202415253324.jpg "Immunohistochemistry: USP7 Antibody (7F10) [NBP3-26258] - Image of USP7 Antibody (7F10) diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: USP7 Antibody (7F10) [NBP3-26258] - Image of USP7 Antibody (7F10) diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26258_rabbit-usp7-mab-7f10-2722024827493.jpg)
![Western Blot: USP7 Antibody (7F10) [NBP3-26258] - Positive Western Blot detected in: K562 whole cell lysate. All lanes: USP7 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 129, 127 kDa Observed band size: 140 kDa](http://images.novusbio.com/fullsize/nbp3-26258_rabbit-usp7-mab-7f10-282202411363024.jpg)
![Immunocytochemistry/Immunofluorescence: USP7 Antibody (7F10) [NBP3-26258] - Staining of Hela Cells with USP7 Antibody (7F10) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26258_rabbit-usp7-mab-7f10-28220241312224.jpg)
![Western Blot: USP7 Antibody (6512) [NBP2-43619] - Analysis of A. 30 ug 293T whole cell extract B. 30 ug A431 whole cell extract C. 30 ug HeLa whole cell extract D. 30 ug HepG2 whole cell extract 5 % SDS-PAGE USP7 antibody [6512] dilution: 1:1000](http://images.novusbio.com/fullsize/USP7-Antibody-6512-Western-Blot-NBP2-43619-img0001.jpg "Western Blot: USP7 Antibody (6512) [NBP2-43619] - Analysis of A. 30 ug 293T whole cell extract B. 30 ug A431 whole cell extract C. 30 ug HeLa whole cell extract D. 30 ug HepG2 whole cell extract 5 % SDS-PAGE USP7 antibody [6512] dilution: 1:1000")
![Immunocytochemistry/Immunofluorescence: USP7 Antibody (6512) [NBP2-43619] - Analysis of Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: USP7 protein stained by USP7 antibody [6512] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/USP7-Antibody-6512-Immunocytochemistry-Immunofluorescence-NBP2-43619-img0002.jpg)
![Immunoprecipitation: USP7 Antibody (6512) [NBP2-43619] - Immunoprecipitation of USP7 protein from HepG2 whole cell extracts using 5 ug of USP7 antibody [6512] Western blot analysis was performed using USP7 antibody [6512] . EasyBlot anti-Mouse IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/USP7-Antibody-6512-Immunoprecipitation-NBP2-43619-img0005.jpg)
![Western Blot: USP7 Antibody (S02-6B8) [NBP3-19800] - Western blot detection of USP7 in C6 cell lysates using NBP3-19800 (1:1000 diluted). Predicted band size: 128kDa. Observed band size: 128kDa.](http://images.novusbio.com/fullsize/USP7-Antibody-S02-6B8-Western-Blot-NBP3-19800-img0002.jpg "Western Blot: USP7 Antibody (S02-6B8) [NBP3-19800] - Western blot detection of USP7 in C6 cell lysates using NBP3-19800 (1:1000 diluted). Predicted band size: 128kDa. Observed band size: 128kDa.")
![Immunohistochemistry-Paraffin: USP7 Antibody (S02-6B8) [NBP3-19800] - Immunohistochemistry of USP7 in paraffin-embedded Human lung cancer tissue using NBP3-19800 at dilution 1/20](http://images.novusbio.com/fullsize/USP7-Antibody-S02-6B8-Immunohistochemistry-Paraffin-NBP3-19800-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: USP7 Antibody (03) [NBP3-06427] - Immunofluorescence staining of Human USP7 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human USP7 monoclonal antibody (1:60) at 4 degrees celsius overnight. Then cells were stained with Alexa Fluor (TM) 488-conjugated Goat Anti-mouse IgG secondary antibody(green). Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/USP7-Antibody-03-Immunocytochemistry-Immunofluorescence-NBP3-06427-img0001.jpg "Immunocytochemistry/Immunofluorescence: USP7 Antibody (03) [NBP3-06427] - Immunofluorescence staining of Human USP7 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human USP7 monoclonal antibody (1:60) at 4 degrees celsius overnight. Then cells were stained with Alexa Fluor (TM) 488-conjugated Goat Anti-mouse IgG secondary antibody(green). Positive staining was localized to cytoplasm and nucleus.")
![Flow Cytometry: USP7 Antibody (03) [NBP3-06427] - Flow cytometric analysis of Human USP7 expression on Jurkat cells. The cells were treated according to manufacturer's manual, stained with purified anti-Human USP7, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/USP7-Antibody-03-Flow-Cytometry-NBP3-06427-img0002.jpg)
![Immunohistochemistry-Paraffin: USP7 Antibody [NBP1-83293] - Staining of human testis shows strong nuclear positivity in cells of seminiferus ducts.](http://images.novusbio.com/fullsize/USP7-Antibody-Immunohistochemistry-Paraffin-NBP1-83293-img0004.jpg "Immunohistochemistry-Paraffin: USP7 Antibody [NBP1-83293] - Staining of human testis shows strong nuclear positivity in cells of seminiferus ducts.")