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![Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody [NBP1-86922] - Staining in human small intestine and liver tissues . Corresponding TPH1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-Immunohistochemistry-Paraffin-NBP1-86922-img0016.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody [NBP1-86922] - Staining in human small intestine and liver tissues . Corresponding TPH1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody [NBP1-86922] - Human melanoma cell line A375 and 5 patient melanoma tissue samples. 15 ug cell lysate per lane. TPH1 antibody at 1:400, 4C O/N. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-Western-Blot-NBP1-86922-img0020.jpg)
![Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody [NBP1-86922] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-Immunohistochemistry-Paraffin-NBP1-86922-img0019.jpg)
![Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody [NBP1-86922] - Staining of human duodenum shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-Immunohistochemistry-Paraffin-NBP1-86922-img0011.jpg)
![Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody [NBP1-86922] - Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-Immunohistochemistry-Paraffin-NBP1-86922-img0013.jpg)
![Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody [NBP1-86922] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-Immunohistochemistry-Paraffin-NBP1-86922-img0015.jpg)
![Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody [NBP1-86922] - Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-Immunohistochemistry-Paraffin-NBP1-86922-img0018.jpg)
![Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody [NB300-176] - Western blot of human dorsal Raphe nucleus showing specifc immunolabeling of the ~55k tryptophan hydroxylase protein.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-Western-Blot-NB300-176-img0002.jpg "Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody [NB300-176] - Western blot of human dorsal Raphe nucleus showing specifc immunolabeling of the ~55k tryptophan hydroxylase protein.")
![Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody (145) [NBP2-90022] - Lane A: THP1 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDaObserved band size:55 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-145-Western-Blot-NBP2-90022-img0002.jpg "Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody (145) [NBP2-90022] - Lane A: THP1 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDaObserved band size:55 kDa(We are unsure as to the identity of these extra bands.)")
![Immunoprecipitation: Tryptophan Hydroxylase 1/TPH-1 Antibody (145) [NBP2-90022] - Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg THP-1 Whole Cell Lysate0.5 uL anti-TPH1 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-TPH1 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody:Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 55 kDaObserved band size: 60 kDa](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-145-Immunoprecipitation-NBP2-90022-img0001.jpg)
![Western Blot: Tryptophan Hydroxylase 1 [p Ser58] Antibody [NB300-260] - Recombinant tryptophan hydroxylase incubated in the absence (Control) and presence of cAMP-dependent protein kinase (PKA) showing specific immunolabeling of the ~53k tryptophan hydroxylase protein phosphorylated at Ser58.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-[p-Ser58]-Antibody-Western-Blot-NB300-260-img0002.jpg "Western Blot: Tryptophan Hydroxylase 1 [p Ser58] Antibody [NB300-260] - Recombinant tryptophan hydroxylase incubated in the absence (Control) and presence of cAMP-dependent protein kinase (PKA) showing specific immunolabeling of the ~53k tryptophan hydroxylase protein phosphorylated at Ser58.")
![Western Blot: Tryptophan Hydroxylase 1 [p Ser260] Antibody [NB300-307] - Rat brainstem lysate showing specific immunolabeling of the ~55k TPH phosphorylated at Ser260. The labeling by Anti-Ser260TPH is specifically blocked by the Ser260 phosphopeptide (Phos-pep) used as antigen. The corresponding nonphosphopeptide (Nonphos-pep) did not block the immunolabeling.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-[p-Ser260]-Antibody-Western-Blot-NB300-307-img0001.jpg "Western Blot: Tryptophan Hydroxylase 1 [p Ser260] Antibody [NB300-307] - Rat brainstem lysate showing specific immunolabeling of the ~55k TPH phosphorylated at Ser260. The labeling by Anti-Ser260TPH is specifically blocked by the Ser260 phosphopeptide (Phos-pep) used as antigen. The corresponding nonphosphopeptide (Nonphos-pep) did not block the immunolabeling.")
![Western Blot: Tryptophan Hydroxylase 1 Overexpression Lysate (Adult Normal) [NBL1-17216] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Tryptophan Hydroxylase 1.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17216-img0002.jpg "Western Blot: Tryptophan Hydroxylase 1 Overexpression Lysate (Adult Normal) [NBL1-17216] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Tryptophan Hydroxylase 1.")
![ELISA: Human Tryptophan Hydroxylase 1/TPH-1 ELISA Kit (Colorimetric) [NBP2-68157] - Samples were spiked with high concentrations of Human Tryptophan Hydroxylase 1/TPH-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-68157_human-tryptophan-hydroxylase-1-tph-1-elisa-kit-color-132202416211345.jpg "ELISA: Human Tryptophan Hydroxylase 1/TPH-1 ELISA Kit (Colorimetric) [NBP2-68157] - Samples were spiked with high concentrations of Human Tryptophan Hydroxylase 1/TPH-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Tryptophan Hydroxylase 1/TPH-1 ELISA Kit (Colorimetric) [NBP2-68157] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-68157_human-tryptophan-hydroxylase-1-tph-1-elisa-kit-color-4320249173451.jpg)
![ELISA: Rat Tryptophan Hydroxylase 1/TPH-1 ELISA Kit (Colorimetric) [NBP2-68167] - Samples were spiked with high concentrations of Rat Tryptophan Hydroxylase 1/TPH-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-68167_rat-tryptophan-hydroxylase-1-tph-1-elisa-kit-colorimetric-132202416175614.jpg "ELISA: Rat Tryptophan Hydroxylase 1/TPH-1 ELISA Kit (Colorimetric) [NBP2-68167] - Samples were spiked with high concentrations of Rat Tryptophan Hydroxylase 1/TPH-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Tryptophan Hydroxylase 1/TPH-1 ELISA Kit (Colorimetric) [NBP2-68167] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-68167_rat-tryptophan-hydroxylase-1-tph-1-elisa-kit-colorimetric-4320249141637.jpg)