Hu,
Mu,
Rt,
Po,
Bv,
Ce,
Ch,
Dr,
Eq,
Pl,
Pm,
ZeSpecies Glossary
Reactivity: Hu, Mu, Rt, Po, Bv, Ce, Ch, Dr, Eq, Pl, Pm, Ze
WB, ICC/IF, IHC, WB
Applications: WB, ICC/IF, IHC, WB
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
![Western Blot: Ubiquitin Antibody (Ubi-1) [NB300-130] - Ubiquitination of PML in response to As2O3. PML-/- MEFs were transiently transfected with the empty vector (EV) or PMLIII (left panel) or contructs encoding PMLIII, PMLIII-SIM or PMLVI (right panel). Two days later, they were treated with As2O3 for 1 h. The cell extracts were immunoprecipitated with rabbit anti-PML antibody and the immunoprecipitate was analyzed by Western blot using anti-ubiquitin and anti-PML antibodies. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0044949) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-Ubi-1-Western-Blot-NB300-130-img0014.jpg "Western Blot: Ubiquitin Antibody (Ubi-1) [NB300-130] - Ubiquitination of PML in response to As2O3. PML-/- MEFs were transiently transfected with the empty vector (EV) or PMLIII (left panel) or contructs encoding PMLIII, PMLIII-SIM or PMLVI (right panel). Two days later, they were treated with As2O3 for 1 h. The cell extracts were immunoprecipitated with rabbit anti-PML antibody and the immunoprecipitate was analyzed by Western blot using anti-ubiquitin and anti-PML antibodies. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0044949) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Ubiquitin Antibody (Ubi-1) [NB300-130] - Study of mutant BRAF protein found within the inclusions. BRAFV600E/ubiquitin double-IF labeling. A nucleus with a large inclusion is shown with positive immunoreactivity for both mutant BRAF (red staining) and ubiquitin (green staining); the merged color yellow (arrow) points out the co-localisation of BRAFV600E and ubiquitin in the same inclusion. Image collected and cropped by CiteAb from the following publication (////doi.org/10.1371/journal.pone.0226199) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-Ubi-1-Immunocytochemistry-Immunofluorescence-NB300-130-img0013.jpg)
![Western Blot: Ubiquitin Antibody (Ubi-1) [NB300-130] - Analysis of HEK293 cell lysates using mouse mAb Ubiquitin, dilution 1:1000 (Green). [1] protein standard (Red), [2] cells maintained in normal medium, [3] cells treated with 10uM of proteasome inhibitor lactacystin (Lc) for 16hrs. Lysed cells were electrophoresed on 4-20% SDS-PAGE and transferred to PVDF membranes. The smear detected above the 200kDa standard represent accumulation of ubiquitinated proteins in proteasome inhibitor-Lc treated cells. The prominent band at 8kDa corresponds to monoubiquitin. Rabbit pAb to HSP60, dilution 1:5000 (Red) was used as a loading control.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-Ubi-1-Western-Blot-NB300-130-img0012.jpg)
![Immunohistochemistry-Paraffin: Ubiquitin Antibody (Ubi-1) [NB300-130] - FFPE section of cerebral cortex of an Alzheimer patient processed with Ubiquitin antibody using HRP/DAB (Brown) and also stained with haemotoxylin (Blue). A typical flame shaped tangle is seen in a pyramidal neuron in the center and is surrounded by some dystrophic neurites, also strongly ubiquitin positive. Both are commonly seen in cortical and hippocampal Alzheimer brain sections and are typical for this disease, but are rare or absent in healthy brain.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-Ubi-1-Immunohistochemistry-Paraffin-NB300-130-img0011.jpg)
![Immunohistochemistry-Paraffin: Ubiquitin Antibody (Ubi-1) [NB300-130] - Staining of Ubiquitin in hippocampal tissue from an Alzheimer patient.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-Ubi-1-Immunohistochemistry-Paraffin-NB300-130-img0008.jpg)
![Western Blot: Ubiquitin Antibody (Ubi-1) [NB300-130] - Molecular Analysis of XopD Ub/Ubl Specificity. S. lycopersicum protein extract blotted for total Ub (Ubiquitin; Novus Biologicals) following a 1 hr room temperature treatment with the XopD variants used in (K) at 1 uM final concentration. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27425412/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-Ubi-1-Western-Blot-NB300-130-img0015.jpg)
![Western Blot: Ubiquitin Antibody (Ubi-1) [NB300-130] - Western blot of Ubiquitin expression on (Lane 1) poly-ubiquitin (lys63 linked), (Lane 2) pure ubiquitin and crude homogenates of adult rat, (Lane 3) cortex, (Lane 4) cerebellum and (Lane 5) brain stem.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-Ubi-1-Western-Blot-NB300-130-img0009.jpg)
![Immunohistochemistry-Paraffin: Ubiquitin Antibody (Ubi-1) [NB300-130] - IHC staining of Ubiquitin in human rectal cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-Ubi-1-Immunohistochemistry-Paraffin-NB300-130-img0010.jpg)
![Western Blot: Ubiquitin Antibody [NB300-129] - Analysis of HEK293 cell lysates using rabbit Ubiquitin antibody, dilution 1:5000 (Green), and mouse beta-Tubulin antibody, dilution 1:10000 (Red) used as a loading control. [1] protein standard (Red), [2] cells maintained in normal medium, [3] cells treated with proteasome inhibitor lactacystin (Lc) at 10uM for 16 hours. Lysed cells were lysed and the lysate subjected to electrophoresis on a 4-20% SDS-PAGE gel, then electrophoretically transferred to PVDF membranes. The smear detected above the 200kDa standard represents accumulations of ubiquitinated proteins in the Lc treated cells. The prominent band at ~8kDa corresponds to monoubiquitin.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-Western-Blot-NB300-129-img0009.jpg "Western Blot: Ubiquitin Antibody [NB300-129] - Analysis of HEK293 cell lysates using rabbit Ubiquitin antibody, dilution 1:5000 (Green), and mouse beta-Tubulin antibody, dilution 1:10000 (Red) used as a loading control. [1] protein standard (Red), [2] cells maintained in normal medium, [3] cells treated with proteasome inhibitor lactacystin (Lc) at 10uM for 16 hours. Lysed cells were lysed and the lysate subjected to electrophoresis on a 4-20% SDS-PAGE gel, then electrophoretically transferred to PVDF membranes. The smear detected above the 200kDa standard represents accumulations of ubiquitinated proteins in the Lc treated cells. The prominent band at ~8kDa corresponds to monoubiquitin.")
![Immunocytochemistry/Immunofluorescence: Ubiquitin Antibody [NB300-129] - Analysis of HeLa cells stained with rabbit pAb to ubiquitin, NB300-129, dilution 1:1,000 in red, and costained with chicken pAb to vimentin, dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. [A] Control HeLa cells maintained in normal medium, [B] HeLa cells treated with 10uM of the proteasome inhibitor lactacystin (Lc) for 24 hours. Proteasomal inhibition leads to formation of strongly ubiquitin positive cytoplasmic inclusions. Note the diffuse cytoplasmic ubiquitin staining in control cells and well defined ubiquitin positive inclusions in the Lc treated cells.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-Immunocytochemistry-Immunofluorescence-NB300-129-img0008.jpg)
![Immunohistochemistry-Paraffin: Ubiquitin Antibody [NB300-129] - IHC staining of Ubiquitin in human rectal cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-Immunohistochemistry-Paraffin-NB300-129-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Ubiquitin Antibody [NB300-129] - Immunofluorescent staining of aggresomes of mouse L-cells treated with lactacysteine, using NB300-129.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-Immunocytochemistry-Immunofluorescence-NB300-129-img0001.jpg)
![Western Blot: Ubiquitin Antibody (1B4-UB) [NB600-776] - GST-ubiquitin detected with Mouse anti-Ubiquitin.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-1B4-UB-Western-Blot-NB600-776-img0002.jpg "Western Blot: Ubiquitin Antibody (1B4-UB) [NB600-776] - GST-ubiquitin detected with Mouse anti-Ubiquitin.")
![Immunohistochemistry-Paraffin: Ubiquitin Antibody (1B4-UB) [NB600-776] - Staining of human tonsil.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-1B4-UB-Immunohistochemistry-Paraffin-NB600-776-img0003.jpg)
![Immunohistochemistry-Paraffin: Ubiquitin Antibody (1B4-UB) [NB600-776] - Human hypophysis.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-1B4-UB-Immunohistochemistry-Paraffin-NB600-776-img0001.jpg)
![Western Blot: Ubiquitin Antibody (P4G7-H11) [NBP1-19306] - Western blot analysis of Ubiquitin: Lane 1: MW marker, Lane 2: Ubiquitin, Lane 3: HeLa, Lane 4: RK-13, Lane 5: 3T3, Lane 6: PC-12, Lane 7: Mouse Brain, Lane 8: Rat Brain, Lane 9: MDBK](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-P4G7-H11-Western-Blot-NBP1-19306-img0002.jpg "Western Blot: Ubiquitin Antibody (P4G7-H11) [NBP1-19306] - Western blot analysis of Ubiquitin: Lane 1: MW marker, Lane 2: Ubiquitin, Lane 3: HeLa, Lane 4: RK-13, Lane 5: 3T3, Lane 6: PC-12, Lane 7: Mouse Brain, Lane 8: Rat Brain, Lane 9: MDBK")
![Immunohistochemistry: Ubiquitin Antibody (P4G7-H11) [NBP1-19306] - Immunohistochemistry analysis of human brain, cortex tissue stained with Ubiquitin, mAb (P4G7-H11) at 5-10ug/ml.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-P4G7-H11-Immunohistochemistry-NBP1-19306-img0001.jpg)
![Flow Cytometry: Ubiquitin Antibody (P4G7-H11) [NBP1-19306] - Flow cytometry analysis of human hepatoma QGY cells analyzed by flow cytometry using isotype control antibody (left) or Ubiquitin Monoclonal Antibody (P4G7-H11) (right) at 10ug/mL.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-P4G7-H11-Flow-Cytometry-NBP1-19306-img0003.jpg)
![Western Blot: Poly-Ubiquitin Antibody (5Y8J0) - K48 Linkage [NBP3-16232] - Western blot analysis of extracts of various cell lines, using Poly-Ubiquitin antibody (5Y8J0) - K48 Linkageat 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Ubiquitin-B-Antibody-5Y8J0-K48-Linkage-Western-Blot-NBP3-16232-img0004.jpg "Western Blot: Poly-Ubiquitin Antibody (5Y8J0) - K48 Linkage [NBP3-16232] - Western blot analysis of extracts of various cell lines, using Poly-Ubiquitin antibody (5Y8J0) - K48 Linkageat 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: Poly-Ubiquitin Antibody (5Y8J0) - K48 Linkage [NBP3-16232] - Immunofluorescence analysis of U-2 OS cells using Poly-Ubiquitin antibody (5Y8J0) - K48 Linkage at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Ubiquitin-B-Antibody-5Y8J0-K48-Linkage-Immunocytochemistry-Immunofluorescence-NBP3-16232-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Poly-Ubiquitin Antibody (5Y8J0) - K48 Linkage [NBP3-16232] - Immunofluorescence analysis of C6 cells using Poly-Ubiquitin antibody (5Y8J0) - K48 Linkage at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Ubiquitin-B-Antibody-5Y8J0-K48-Linkage-Immunocytochemistry-Immunofluorescence-NBP3-16232-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Poly-Ubiquitin Antibody (5Y8J0) - K48 Linkage [NBP3-16232] - Immunofluorescence analysis of NIH-3T3 cells using Poly-Ubiquitin antibody (5Y8J0) - K48 Linkage at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Ubiquitin-B-Antibody-5Y8J0-K48-Linkage-Immunocytochemistry-Immunofluorescence-NBP3-16232-img0002.jpg)
![Western Blot: Ubiquitin Antibody (3E2-E6) [H00006233-M01] - RPS27A monoclonal antibody (M01), clone 3E2-E6 Analysis of RPS27A expression in HL-60.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-3E2-E6-Western-Blot-H00006233-M01-img0002.jpg "Western Blot: Ubiquitin Antibody (3E2-E6) [H00006233-M01] - RPS27A monoclonal antibody (M01), clone 3E2-E6 Analysis of RPS27A expression in HL-60.")
![Western Blot: Poly-Ubiquitin Antibody (HWA4C4) - K63 Linkage [NBP2-36523] - Poly-Ubiquitin K63 Linkage Antibody (HWA4C4) [NBP2-36523] - Analysis of HeLa S100 Fraction. (Lane A) and MAb to Polyubiquitinylated Conjugates (clone FK1) (Lane B) at 1:1000 dilution.](http://images.novusbio.com/fullsize/Poly-Ubiquitin-K63-Linkage-Antibody-HWA4C4-Western-Blot-NBP2-36523-img0019.jpg "Western Blot: Poly-Ubiquitin Antibody (HWA4C4) - K63 Linkage [NBP2-36523] - Poly-Ubiquitin K63 Linkage Antibody (HWA4C4) [NBP2-36523] - Analysis of HeLa S100 Fraction. (Lane A) and MAb to Polyubiquitinylated Conjugates (clone FK1) (Lane B) at 1:1000 dilution.")
![Immunohistochemistry-Paraffin: Poly-Ubiquitin Antibody (HWA4C4) - K63 Linkage [NBP2-36523] - Poly-Ubiquitin K63 Linkage Antibody (HWA4C4) [NBP2-36523] - Sections of hippocampus from human Alzheimers disease brain stained at 1:100 dilution with biotinylated secondary antibody and DAB detection.](http://images.novusbio.com/fullsize/Poly-Ubiquitin-K63-Linkage-Antibody-HWA4C4-Immunohistochemistry-Paraffin-NBP2-36523-img0017.jpg)
![Western Blot: Poly-Ubiquitin Antibody (HWA4C4) - K63 Linkage [NBP2-36523] - Poly-Ubiquitin K63 Linkage Antibody (HWA4C4) [NBP2-36523] - Analysis at 1:1000 dilution](http://images.novusbio.com/fullsize/Poly-Ubiquitin-K63-Linkage-Antibody-HWA4C4-Western-Blot-NBP2-36523-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Ubiquitin Antibody (FK1) [NBP3-11039] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone FK1. Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 60X.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-FK1-Immunocytochemistry-Immunofluorescence-NBP3-11039-img0001.jpg "Immunocytochemistry/Immunofluorescence: Ubiquitin Antibody (FK1) [NBP3-11039] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone FK1. Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 60X.")
![Western Blot: Poly-Ubiquitin Antibody - K11 Linkage [NBP3-05681] - Analysis of extracts of various cell lines, using Poly-Ubiquitin Rabbit pAb (NBP3-05681) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Poly-Ubiquitin-Antibody---K11-Linkage-Western-Blot-NBP3-05681-img0001.jpg "Western Blot: Poly-Ubiquitin Antibody - K11 Linkage [NBP3-05681] - Analysis of extracts of various cell lines, using Poly-Ubiquitin Rabbit pAb (NBP3-05681) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.")
![Dot Blot: Poly-Ubiquitin Antibody - K11 Linkage [NBP3-05681] - Analysis of all sorts of peptides using Poly-Ubiquitin antibody (NBP3-05681) at 1:1000 dilution.](http://images.novusbio.com/fullsize/Poly-Ubiquitin-Antibody---K11-Linkage-Dot-Blot-NBP3-05681-img0002.jpg)
![Western Blot: Poly-Ubiquitin Antibody - K33 Linkage [NBP3-05656] - Western blot analysis of extracts of various cell lines, using Poly-Ubiquitin Rabbit pAb (NBP3-05656) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Poly-Ubiquitin-Antibody---K33-Linkage-Western-Blot-NBP3-05656-img0001.jpg "Western Blot: Poly-Ubiquitin Antibody - K33 Linkage [NBP3-05656] - Western blot analysis of extracts of various cell lines, using Poly-Ubiquitin Rabbit pAb (NBP3-05656) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.")
![Dot Blot: Poly-Ubiquitin Antibody - K33 Linkage [NBP3-05656] - Dot-blot analysis of all sorts of peptides using Poly-Ubiquitin antibody (NBP3-05656) at 1:1000 dilution.](http://images.novusbio.com/fullsize/Poly-Ubiquitin-Antibody---K33-Linkage-Dot-Blot-NBP3-05656-img0002.jpg)
![Western Blot: Poly-Ubiquitin Antibody [NBP3-05664] - Western blot analysis of extracts of various cell lines, using Poly-Ubiquitin Rabbit pAb (NBP3-05664) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Poly-Ubiquitin-Antibody-Western-Blot-NBP3-05664-img0001.jpg "Western Blot: Poly-Ubiquitin Antibody [NBP3-05664] - Western blot analysis of extracts of various cell lines, using Poly-Ubiquitin Rabbit pAb (NBP3-05664) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.")
![Dot Blot: Poly-Ubiquitin Antibody [NBP3-05664] - Dot-blot analysis of all sorts of peptides using Poly-Ubiquitin antibody (NBP3-05664) at 1:1000 dilution.](http://images.novusbio.com/fullsize/Poly-Ubiquitin-Antibody-Dot-Blot-NBP3-05664-img0002.jpg)
![Western Blot: Poly-Ubiquitin Antibody - M1 Linkage [NBP3-05631] - Western blot analysis of extracts of various cell lines, using Poly-Ubiquitin antibody (NBP3-05631) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Poly-Ubiquitin-Antibody---M1-Linkage-Western-Blot-NBP3-05631-img0001.jpg "Western Blot: Poly-Ubiquitin Antibody - M1 Linkage [NBP3-05631] - Western blot analysis of extracts of various cell lines, using Poly-Ubiquitin antibody (NBP3-05631) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Dot Blot: Poly-Ubiquitin Antibody - M1 Linkage [NBP3-05631] - Dot-blot analysis of all sorts of peptides using Poly-Ubiquitin antibody (NBP3-05631) at 1:1000 dilution.](http://images.novusbio.com/fullsize/Poly-Ubiquitin-Antibody---M1-Linkage-Dot-Blot-NBP3-05631-img0002.jpg)