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![Western Blot: PrPC Antibody [NB100-56605] - Analysis of BSE in (A) recombinant fusion protein containing amino acids 162-170 and (B) fusion partner without these amino acids, using this antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/Prion-protein-Antibody-Western-Blot-NB100-56605-img0002.jpg "Western Blot: PrPC Antibody [NB100-56605] - Analysis of BSE in (A) recombinant fusion protein containing amino acids 162-170 and (B) fusion partner without these amino acids, using this antibody at 5 ug/ml.")
![Western Blot: PrPC Antibody [NB100-56604] - Analysis of BSE in (A) recombinant fusion protein containing amino acids 142-148 and (B) fusion partner without these amino acids, using this antibody. 5 ug/ml.](http://images.novusbio.com/fullsize/Prion-protein-Antibody-Western-Blot-NB100-56604-img0002.jpg "Western Blot: PrPC Antibody [NB100-56604] - Analysis of BSE in (A) recombinant fusion protein containing amino acids 142-148 and (B) fusion partner without these amino acids, using this antibody. 5 ug/ml.")
![Western Blot: PrPC Antibody [NB100-56606] - Analysis of BSE in (A) recombinant fusion protein containing amino acids 217-229 and (B) fusion partner without these amino acids, using this antibody. 5 ug/ml.](http://images.novusbio.com/fullsize/Prion-protein-Antibody-Western-Blot-NB100-56606-img0002.jpg "Western Blot: PrPC Antibody [NB100-56606] - Analysis of BSE in (A) recombinant fusion protein containing amino acids 217-229 and (B) fusion partner without these amino acids, using this antibody. 5 ug/ml.")
![Immunocytochemistry/Immunofluorescence: PrPC Antibody [NBP2-38508] - Staining of human cell line U-2 OS shows localization to nuclear membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Prion-protein-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38508-img0003.jpg "Immunocytochemistry/Immunofluorescence: PrPC Antibody [NBP2-38508] - Staining of human cell line U-2 OS shows localization to nuclear membrane & cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: PrPC Antibody [NBP2-38508] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/PrPC-Antibody-Immunohistochemistry-Paraffin-NBP2-38508-img0007.jpg)
![Immunohistochemistry-Paraffin: PrPC Antibody [NBP2-38508] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/Prion-protein-Antibody-Immunohistochemistry-NBP2-38508-img0002.jpg)
![Immunohistochemistry-Paraffin: PrPC Antibody [NBP2-38508] - Staining of human cerebral cortex shows strong granuclar cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/PrPC-Antibody-Immunohistochemistry-Paraffin-NBP2-38508-img0005.jpg)
![Immunohistochemistry-Paraffin: PrPC Antibody [NBP2-38508] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/PrPC-Antibody-Immunohistochemistry-Paraffin-NBP2-38508-img0006.jpg)
![Western Blot: PrPSc Antibody (3F4) [NBP2-81078] - Human brain cerebral cortex (A), cerebellum (B), and hippocampus (C) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric mouse IgG1 version of PrPSc antibody (3F4) [NBP2-81078]. Cerebral cortex and hippocampus samples were probed using 0.001 ug/ml of NBP2-81078, and cerebellum samples with 0.003 ug/ml of NBP2-81078, before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted running size for unmodified major Prion protein is 27.7 kDa though this protein has several glycosylated forms, may be lipidated, sumoylated and post-translationally cleaved to produce the matrue form of the protein [Uniprot]. NBP2-81078 successfully detected major Prion protein in human brain cerebral cortex, cerebellum, and hippocampus lysates.](http://images.novusbio.com/fullsize/Prion-protein-Antibody-3F4-Western-Blot-NBP2-81078-img0002.jpg "Western Blot: PrPSc Antibody (3F4) [NBP2-81078] - Human brain cerebral cortex (A), cerebellum (B), and hippocampus (C) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric mouse IgG1 version of PrPSc antibody (3F4) [NBP2-81078]. Cerebral cortex and hippocampus samples were probed using 0.001 ug/ml of NBP2-81078, and cerebellum samples with 0.003 ug/ml of NBP2-81078, before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted running size for unmodified major Prion protein is 27.7 kDa though this protein has several glycosylated forms, may be lipidated, sumoylated and post-translationally cleaved to produce the matrue form of the protein [Uniprot]. NBP2-81078 successfully detected major Prion protein in human brain cerebral cortex, cerebellum, and hippocampus lysates.")
![Immunocytochemistry/Immunofluorescence: PrPSc Antibody (3F4) [NBP2-81078] - Immunofluorescence staining of fixed U251 cells with [NBP2-81078]. Immunofluorescence analysis of paraformaldehyde fixed U251 cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of 3F4 [NBP2-81078] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81078, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Prion-protein-Antibody-3F4-Immunocytochemistry-Immunofluorescence-NBP2-81078-img0001.jpg)
![Western Blot: PrPC Antibody (S03-2E2) [NBP3-19967] - Western blot detection of PrPC in Rat Brain and Mouse Brain lysates using NBP3-19967 (1:1000 diluted). Predicted band size: 28kDa. Observed band size: 28kDa.](http://images.novusbio.com/fullsize/PrPC-Antibody-S03-2E2-Western-Blot-NBP3-19967-img0002.jpg "Western Blot: PrPC Antibody (S03-2E2) [NBP3-19967] - Western blot detection of PrPC in Rat Brain and Mouse Brain lysates using NBP3-19967 (1:1000 diluted). Predicted band size: 28kDa. Observed band size: 28kDa.")
![Immunohistochemistry-Paraffin: PrPC Antibody (S03-2E2) [NBP3-19967] - Immunohistochemistry of Prion protein in paraffin-embedded Human breast cancer tissue using NBP3-19967 at dilution 1/20](http://images.novusbio.com/fullsize/PrPC-Antibody-S03-2E2-Immunohistochemistry-Paraffin-NBP3-19967-img0001.jpg)
![Western Blot: PrPSc Antibody (V21-V) [NBP1-71678] - Western blot analysis of prion protein was performed by Drs. Valeriy Ostapchenko and Marco Prado, Robarts Research Institute, the University of Western Ontario, London, Ontario, Canada. Lanes A-C represent extracts (30 ug of total protein per lane) of PrP-KO CF-10 cells (A), mouse hippocampus (B) and HEK293 cells, transfected with mouse prion protein bearing 3F4 epitope (C); lane D corresponds to 30 ng recombinant MoPrP.](http://images.novusbio.com/fullsize/Prion-protein-Antibody-V21-V-Western-Blot-NBP1-71678-img0006.jpg "Western Blot: PrPSc Antibody (V21-V) [NBP1-71678] - Western blot analysis of prion protein was performed by Drs. Valeriy Ostapchenko and Marco Prado, Robarts Research Institute, the University of Western Ontario, London, Ontario, Canada. Lanes A-C represent extracts (30 ug of total protein per lane) of PrP-KO CF-10 cells (A), mouse hippocampus (B) and HEK293 cells, transfected with mouse prion protein bearing 3F4 epitope (C); lane D corresponds to 30 ng recombinant MoPrP.")
![Western Blot: PrPSc Antibody (V21-V) [NBP1-71678] - Western blot analysis of prion protein in mouse brain extract (A), HEK293 cells producing recombinant human prion protein (B), and untransfected HEK 293 cells (C). 200 ug of total protein loaded per well.](http://images.novusbio.com/fullsize/Prion-protein-Antibody-V21-V-Western-Blot-NBP1-71678-img0005.jpg)
![Western Blot: PrPC Antibody (T16-R) [NBP1-71667] - PrPC protein in mouse brain extract (A), HEK293 cells producing recombinant human prion protein (B), and cells transfected with empty vector (C). 200 ug of total protein loaded per well.](http://images.novusbio.com/fullsize/Prion-protein-Antibody-T16-R-Western-Blot-NBP1-71667-img0008.jpg "Western Blot: PrPC Antibody (T16-R) [NBP1-71667] - PrPC protein in mouse brain extract (A), HEK293 cells producing recombinant human prion protein (B), and cells transfected with empty vector (C). 200 ug of total protein loaded per well.")
![Western Blot: PrPC Antibody (3F4) [NBP3-23335] - Western blot of GST recombinant human full-length prion protein that was untreated (lanes 1 and 3) or phosphorylated with Cdk5/p25 (lanes 2 & 4). Endogenous prion phosphorylation was examined in human PC3 cells untreated (lanes 5 & 7) or treated with Calyculin A (100 nM) for 30 min (lanes 6 & 8). The blots were probed with anti-Prion protein (3F4) (lanes 1, 2, 5, & 6) or anti-Prion protein (Ser-43) (lanes 3, 4, 7, & 8).](http://images.novusbio.com/fullsize/nbp3-23335_mouse-prpc-mab-3f4-19920231911475.jpg "Western Blot: PrPC Antibody (3F4) [NBP3-23335] - Western blot of GST recombinant human full-length prion protein that was untreated (lanes 1 and 3) or phosphorylated with Cdk5/p25 (lanes 2 & 4). Endogenous prion phosphorylation was examined in human PC3 cells untreated (lanes 5 & 7) or treated with Calyculin A (100 nM) for 30 min (lanes 6 & 8). The blots were probed with anti-Prion protein (3F4) (lanes 1, 2, 5, & 6) or anti-Prion protein (Ser-43) (lanes 3, 4, 7, & 8).")
![Western Blot: PrPC [p Ser43] Antibody [NBP3-23337] - Western blot of GST recombinant human full-length prion protein that was untreated (lanes 1 and 3) or phosphorylated with Cdk5/p25 (lanes 2 & 4). Endogenous prion phosphorylation was examined in human PC3 cells untreated (lanes 5 & 7) or treated with Calyculin A (100 nM) for 30 min (lanes 6 & 8). The blots were probed with anti-Prion protein (3F4) (lanes 1, 2, 5, & 6) or anti-Prion protein (Ser-43) (lanes 3, 4, 7, & 8).](http://images.novusbio.com/fullsize/nbp3-23337_rabbit-prpc-p-ser43-pab-19920231410133.jpg "Western Blot: PrPC [p Ser43] Antibody [NBP3-23337] - Western blot of GST recombinant human full-length prion protein that was untreated (lanes 1 and 3) or phosphorylated with Cdk5/p25 (lanes 2 & 4). Endogenous prion phosphorylation was examined in human PC3 cells untreated (lanes 5 & 7) or treated with Calyculin A (100 nM) for 30 min (lanes 6 & 8). The blots were probed with anti-Prion protein (3F4) (lanes 1, 2, 5, & 6) or anti-Prion protein (Ser-43) (lanes 3, 4, 7, & 8).")
![Western Blot: PrPC Antibody (3D0J9) [NBP3-16708] - Western blot analysis of extracts of various cell lines, using PrPC Rabbit mAb (NBP3-16708) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/PrPC-Antibody-3D0J9-Western-Blot-NBP3-16708-img0001.jpg "Western Blot: PrPC Antibody (3D0J9) [NBP3-16708] - Western blot analysis of extracts of various cell lines, using PrPC Rabbit mAb (NBP3-16708) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")