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![Western Blot: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Analysis of Protein Disulfide Isomerase/P4HB was performed by loading 25 ug of HepG2 (Lane 1), Hela (Lane 2) and NIH-3T3 (Lane 3) cell lysates.](http://images.novusbio.com/fullsize/PDI-Antibody-RL90-Western-Blot-NB300-517-img0042.jpg "Western Blot: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Analysis of Protein Disulfide Isomerase/P4HB was performed by loading 25 ug of HepG2 (Lane 1), Hela (Lane 2) and NIH-3T3 (Lane 3) cell lysates.")
![Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Staining of Phalloidin (purple) and PDI (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a Protein Disulfide Isomerase/P4HB monoclonal antibody at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 650 Phalloidin at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst at a concentration of 1ug/ml for 30 minutes.](http://images.novusbio.com/fullsize/PDI-Antibody-RL90-Immunocytochemistry-Immunofluorescence-NB300-517-img0032.jpg)
![Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Both normal and cancer biopsies of deparaffinized Human colon tissue tissues.](http://images.novusbio.com/fullsize/PDI-Antibody-RL90-Immunohistochemistry-Paraffin-NB300-517-img0041.jpg)
![Flow Cytometry: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Analysis of Hela cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/PDI-Antibody-RL90-Flow-Cytometry-NB300-517-img0039.jpg)
![Western Blot: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Analysis of PDIA2 expression in 1) human brain and 2) rat liver tissues extracts, 3) HeLa and 4) Hek 293 whole cell lysates.](http://images.novusbio.com/fullsize/PDI-Antibody-RL90-Western-Blot-NB300-517-img0036.jpg)
![Western Blot: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Cell surface Protein Disulfide Isomerase/P4HB associated with GC-AC. Proteins were pulled down with anti GC-A antibody from HUVEC or HMC membrane preparations and detected with anti-Protein Disulfide Isomerase/P4HB antibody (RL90)compared to control IP (no anti GC-A) and non-immunoprecipitated proteins from HMC and HUVEC. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0112986), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PDI-Antibody-RL90-Western-Blot-NB300-517-img0043.jpg)
![Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Staining in p19 Cells.](http://images.novusbio.com/fullsize/PDI-Antibody-RL90-Immunocytochemistry-Immunofluorescence-NB300-517-img0031.jpg)
![Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a Protein Disulfide Isomerase/P4HB monoclonal antibody at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with Dylight 488 goat anti-mouse IgG secondary antibody at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with Dylight 350 Phalloidin at a dilution of 1:120 (2.5units/ml final concentration) and nuclei (red) were stained with DRAQ5 at a concentration of 1ug/ml for 30 minutes.](http://images.novusbio.com/fullsize/PDI-Antibody-RL90-Immunocytochemistry-Immunofluorescence-NB300-517-img0025.jpg)
![Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a Protein Disulfide Isomerase/P4HB monoclonal antibody at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 550 goat anti-mouse IgG secondary antibody at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 488 Phalloidin at a dilution of 1:300 (1 unit/ml final concentration) for 30 minutes.](http://images.novusbio.com/fullsize/PDI-Antibody-RL90-Immunocytochemistry-Immunofluorescence-NB300-517-img0026.jpg)
![Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a Protein Disulfide Isomerase/P4HB monoclonal antibody at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 550 Phalloidin at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst at a concentration of 1ug/ml for 30 minutes.](http://images.novusbio.com/fullsize/PDI-Antibody-RL90-Immunocytochemistry-Immunofluorescence-NB300-517-img0027.jpg)
![Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Staining in A549 Cells.](http://images.novusbio.com/fullsize/PDI-Antibody-RL90-Immunocytochemistry-Immunofluorescence-NB300-517-img0028.jpg)
![Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Staining in HMVEC Cells.](http://images.novusbio.com/fullsize/PDI-Antibody-RL90-Immunocytochemistry-Immunofluorescence-NB300-517-img0029.jpg)
![Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Staining in NS-1 Cells.](http://images.novusbio.com/fullsize/PDI-Antibody-RL90-Immunocytochemistry-Immunofluorescence-NB300-517-img0030.jpg)
![Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Cancer biopsies of deparaffinized Human lung adenocarcinoma tissues.](http://images.novusbio.com/fullsize/PDI-Antibody-RL90-Immunohistochemistry-Paraffin-NB300-517-img0035.jpg)
![Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.](http://images.novusbio.com/fullsize/PDI-Antibody-RL90-Immunohistochemistry-Paraffin-NB300-517-img0040.jpg)
![Flow Cytometry: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Analysis of NIH/3T3 cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/PDI-Antibody-RL90-Flow-Cytometry-NB300-517-img0037.jpg)
![Flow Cytometry: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Analysis of K562 cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/PDI-Antibody-RL90-Flow-Cytometry-NB300-517-img0038.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Down regulated PDIA2 attenuates CNP-mediated cGMP activation in HMCs. Western blotting with RL90 antibody shows partial knockdown of Protein Disulfide Isomerase/P4HB in HMCs.](http://images.novusbio.com/fullsize/PDI-Antibody-RL90-Knockdown-Validated-NB300-517-img0044.jpg)
![P. gingivalis and its OMVs differentially induce inflammasome signaling and pyroptosis in murine macrophages. BMM were infected as before (2 h at MOI of 25:1, see Materials and Methods) with viable P. gingivalis (Pg), heat-killed-Pg (HK-Pg), OMVs, or heat-inactivated-OMVs (HI-OMVs) and (A) the activation of inflammasome components in the lysates [or supernatants (sup) where indicated] measured after 24 h by Western blot; beta -actin serves as a loading control throughout. Western blot data are representative of at least three independent experiments. (B) Production of IL-1 beta and IL-18 (by ELISA) from BMM was measured in the supernatant at 24 h. Data are mean ± SD from four independent experiments. Cell viability was determined by measuring extracellular LDH release and by 7-AAD exclusion (by flow-cytometry) at 24 h. 7-AAD is a membrane impermeant dye that is excluded from viable cells. The percent of 7-AAD positive (±SD) cells from three independent experiments was calculated relative to the untreated control. BMM treated with H2O2 (1 mM for 60 min) were included as positive control for 7-AAD. A representative histogram is shown. Cells were also treated where indicated with OMVs in the presence of KYT-1 and KYT-36 (10 μM of each). *p < 0.05. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/28824884), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-517_mouse-monoclonal-pdi-antibody-rl90-23220248194315.jpg)
![Western Blot: Protein Disulfide Isomerase/P4HB Antibody [NBP1-97470] - Analysis of PDI: Lane 1: MWM, Lane 2: Protein Disulfide Isomerase/P4HB (human), (recombinant) (His-tag) (Prod. No.ADI-SPP-891), Lane 3: HeLa, (cell lysate), Lane 4: Rat Testis Tissue Lysate, Lane 5: Mouse Spleen Tissue Lysate.](http://images.novusbio.com/fullsize/PDI-Antibody-Western-Blot-NBP1-97470-img0001.jpg "Western Blot: Protein Disulfide Isomerase/P4HB Antibody [NBP1-97470] - Analysis of PDI: Lane 1: MWM, Lane 2: Protein Disulfide Isomerase/P4HB (human), (recombinant) (His-tag) (Prod. No.ADI-SPP-891), Lane 3: HeLa, (cell lysate), Lane 4: Rat Testis Tissue Lysate, Lane 5: Mouse Spleen Tissue Lysate.")
![Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-97470] - Analysis of human spleen tissue stained with Protein Disulfide Isomerase/P4HB, pAb at 10ug/ml.](http://images.novusbio.com/fullsize/PDI-Antibody-Immunohistochemistry-Paraffin-NBP1-97470-img0002.jpg)
![Western Blot: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Analysis of 25 ug of HepG2 (Lane 1), Hela (Lane 2) and NIH-3T3 (Lane 3) cell lysates.](http://images.novusbio.com/fullsize/PDI-Antibody-RL77-Western-Blot-NB600-1164-img0031.jpg "Western Blot: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Analysis of 25 ug of HepG2 (Lane 1), Hela (Lane 2) and NIH-3T3 (Lane 3) cell lysates.")
![Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Analysis of Protein Disulfide Isomerase/P4HB using monoclonal antibody shows staining in A549 Cells.](http://images.novusbio.com/fullsize/PDI-Antibody-RL77-Immunocytochemistry-Immunofluorescence-NB600-1164-img0027.jpg)
![Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues.](http://images.novusbio.com/fullsize/PDI-Antibody-RL77-Immunohistochemistry-Paraffin-NB600-1164-img0030.jpg)
![Flow Cytometry: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Analysis of 3T3 cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/PDI-Antibody-RL77-Flow-Cytometry-NB600-1164-img0024.jpg)
![Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Staining of rat epithelial cells.](http://images.novusbio.com/fullsize/PDI-Antibody-RL77-Immunocytochemistry-Immunofluorescence-NB600-1164-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Analysis of NS-1 Cells.](http://images.novusbio.com/fullsize/PDI-Antibody-RL77-Immunocytochemistry-Immunofluorescence-NB600-1164-img0025.jpg)
![Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Analysis shows staining in HMVEC Cells.](http://images.novusbio.com/fullsize/PDI-Antibody-RL77-Immunocytochemistry-Immunofluorescence-NB600-1164-img0026.jpg)
![Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Normal deparaffinized Human tonsil tissue tissues.](http://images.novusbio.com/fullsize/PDI-Antibody-RL77-Immunohistochemistry-Paraffin-NB600-1164-img0028.jpg)
![Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Both normal and cancer biopsies of deparaffinized Human liver tissues.](http://images.novusbio.com/fullsize/PDI-Antibody-RL77-Immunohistochemistry-Paraffin-NB600-1164-img0029.jpg)
![Flow Cytometry: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Analysis of K562 cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/PDI-Antibody-RL77-Flow-Cytometry-NB600-1164-img0022.jpg)
![Flow Cytometry: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Analysis of Hela cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/PDI-Antibody-RL77-Flow-Cytometry-NB600-1164-img0023.jpg)
![Western Blot: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P] - (C) Immunoblots for quantitation of steady-state protein levels of collagen modifying enzymes and chaperones in proband and control cells. Proband fibroblasts show increases in PDI, LH1, LH2 and BiP protein levels. CyPB and FKBP65, both collagen-interacting isomerases, are consistently decreased in proband fibroblasts and osteoblasts. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27441836) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Western-Blot-H00005034-B01P-img0009.jpg "Western Blot: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P] - (C) Immunoblots for quantitation of steady-state protein levels of collagen modifying enzymes and chaperones in proband and control cells. Proband fibroblasts show increases in PDI, LH1, LH2 and BiP protein levels. CyPB and FKBP65, both collagen-interacting isomerases, are consistently decreased in proband fibroblasts and osteoblasts. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27441836) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P] - Analysis of purified antibody to P4HB on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Immunocytochemistry-Immunofluorescence-H00005034-B01P-img0005.jpg)
![Western Blot: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P] - Analysis of P4HB expression in HeLa.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Western-Blot-H00005034-B01P-img0006.jpg)
![Western Blot: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P] - Analysis of P4HB expression in transfected 293T cell line by P4HB polyclonal antibody. Lane 1: P4HB transfected lysate(55.88 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Western-Blot-H00005034-B01P-img0008.jpg)
![Western Blot: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P] - Analysis of P4HB expression in human liver.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Western-Blot-H00005034-B01P-img0007.jpg)
![Western Blot: Protein Disulfide Isomerase/P4HB Antibody [NBP3-03678] - Western blot analysis of extracts of various cell lines, using Protein Disulfide Isomerase/P4HB antibody (NBP3-03678) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Western-Blot-NBP3-03678-img0011.jpg "Western Blot: Protein Disulfide Isomerase/P4HB Antibody [NBP3-03678] - Western blot analysis of extracts of various cell lines, using Protein Disulfide Isomerase/P4HB antibody (NBP3-03678) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody [NBP3-03678] - Immunofluorescence analysis of U2OS cells using Protein Disulfide Isomerase/P4HB Rabbit pAb (NBP3-03678) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03678-img0010.jpg)
![Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP3-03678] - Rat kidney using P4HB antibody at dilution of 1:150 (40x lens).](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Immunohistochemistry-Paraffin-NBP3-03678-img0008.jpg)
![Western Blot: Protein Disulfide Isomerase/P4HB Antibody [NBP3-03678] - Analysis of 200ug extracts of SW620 cells using Protein Disulfide Isomerase/P4HB antibody . Western blot was performed from the immunoprecipitate using this antibody.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Western-Blot-NBP3-03678-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody [NBP3-03678] - Analysis of U-2OS cells using Protein Disulfide Isomerase/P4HB antibody at dilution of 1:400. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03678-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody [NBP3-03678] - Immunofluorescence analysis of NIH/3T3 cells using Protein Disulfide Isomerase/P4HB Rabbit pAb (NBP3-03678) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03678-img0009.jpg)
![Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP3-03678] - Human lung cancer using P4HB antibody at dilution of 1:150 (40x lens).](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Immunohistochemistry-Paraffin-NBP3-03678-img0006.jpg)
![Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP3-03678] - Mouse brain using P4HB antibody at dilution of 1:150 (40x lens).](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Immunohistochemistry-Paraffin-NBP3-03678-img0007.jpg)
![Western Blot: Protein Disulfide Isomerase/P4HB Antibody [NBP2-41319] - Analysis of PDIA1 in rat small intestine tissue lysate with PDIA1 antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Western-Blot-NBP2-41319-img0001.jpg "Western Blot: Protein Disulfide Isomerase/P4HB Antibody [NBP2-41319] - Analysis of PDIA1 in rat small intestine tissue lysate with PDIA1 antibody at (A) 1 and (B) 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody [NBP2-41319] - Immunofluorescence of Protein Disulfide Isomerase/P4HB in rat small intestine tissue with Protein Disulfide Isomerase/P4HB antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Immunofluorescence-NBP2-41319-img0003.jpg)
![Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP2-41319] - Rat small intestine tissue at 5 ug/ml.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Immunohistochemistry-NBP2-41319-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (023) [NBP2-90660] - Immunofluorescence staining of P4HB in NIH/3T3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-mouse P4HB monoclonal antibody (1:60) at 4? overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-023-Immunocytochemistry-Immunofluorescence-NBP2-90660-img0004.jpg "Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (023) [NBP2-90660] - Immunofluorescence staining of P4HB in NIH/3T3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-mouse P4HB monoclonal antibody (1:60) at 4? overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).")
![Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody (023) [NBP2-90660] - Immunochemical staining of mouse P4HB in mouse brain with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-023-Immunohistochemistry-Paraffin-NBP2-90660-img0003.jpg)
![Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody (023) [NBP2-90660] - Immunochemical staining of mouse P4HB in mouse liver with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-023-Immunohistochemistry-Paraffin-NBP2-90660-img0001.jpg)
![Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody (023) [NBP2-90660] - Immunochemical staining of mouse P4HB in mouse intestine with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-023-Immunohistochemistry-Paraffin-NBP2-90660-img0002.jpg)
![Western Blot: Protein Disulfide Isomerase/P4HB Antibody (16) [NBP2-89676] - Lane A: MCF7 Whole Cell Lysate Lane B: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique.Performed under reducing conditions. Predicted band size:57 kDaObserved band size:57 kDa (We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-16-Western-Blot-NBP2-89676-img0003.jpg "Western Blot: Protein Disulfide Isomerase/P4HB Antibody (16) [NBP2-89676] - Lane A: MCF7 Whole Cell Lysate Lane B: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique.Performed under reducing conditions. Predicted band size:57 kDaObserved band size:57 kDa (We are unsure as to the identity of these extra bands.)")
![Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (16) [NBP2-89676] - Staining of Human P4HB in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human P4HB monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to endoplasmic reticulum.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-16-Immunocytochemistry-Immunofluorescence-NBP2-89676-img0001.jpg)
![Immunoprecipitation: Protein Disulfide Isomerase/P4HB Antibody (16) [NBP2-89676] - Lane A:0.5 mg MCF-7 Whole Cell Lysate 2 uL anti-P4HB rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-P4HB rabbit monoclonal antibody,at 1:200 dilution Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 57 kDa Observed band size: 57 kDa](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-16-Immunoprecipitation-NBP2-89676-img0002.jpg)
![Western Blot: Protein Disulfide Isomerase/P4HB Antibody [NBP1-57949] - HepG2 tissue lysate at a concentration of 0.5ug/ml.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Western-Blot-NBP1-57949-img0005.jpg "Western Blot: Protein Disulfide Isomerase/P4HB Antibody [NBP1-57949] - HepG2 tissue lysate at a concentration of 0.5ug/ml.")
![Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-57949] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Immunohistochemistry-Paraffin-NBP1-57949-img0004.jpg)
![Western Blot: P4HB Overexpression Lysate (Adult Normal) [NBP2-04183] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for P4HB.](http://images.novusbio.com/fullsize/P4HB-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04183-img0001.jpg "Western Blot: P4HB Overexpression Lysate (Adult Normal) [NBP2-04183] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for P4HB.")
![ELISA: Human Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric) [NBP2-81222] - Samples were spiked with high concentrations of Human Protein Disulfide Isomerase/P4HB and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-81222_human-protein-disulfide-isomerase-p4hb-elisa-kit-color-132202416173435.jpg "ELISA: Human Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric) [NBP2-81222] - Samples were spiked with high concentrations of Human Protein Disulfide Isomerase/P4HB and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric) [NBP2-81222] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-81222_human-protein-disulfide-isomerase-p4hb-elisa-kit-color-432024941535.jpg)
![ELISA: Mouse Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric) [NBP2-81223] - Samples were spiked with high concentrations of Mouse Protein Disulfide Isomerase/P4HB and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-81223_mouse-protein-disulfide-isomerase-p4hb-elisa-kit-color-13220241613369.jpg "ELISA: Mouse Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric) [NBP2-81223] - Samples were spiked with high concentrations of Mouse Protein Disulfide Isomerase/P4HB and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric) [NBP2-81223] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-81223_mouse-protein-disulfide-isomerase-p4hb-elisa-kit-color-43202495080.jpg)