| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | IC1997P | |||
| Carrier Free | NL1997G | |||
| Carrier Free | AF1997-SP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 488 | IC2729G | |||
| Allophycocyanin | IC2729A | |||
| Biotin | BAF2729 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | IC2729A | |||
| Carrier Free | IC2729G | |||
| Carrier Free | AF2729-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-04320SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-13177SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47427SS | |||
| Azide and BSA Free | NBP2-80865 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47427 | |||
| BSA Free | NBP1-47427SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77109-0.025mg | |||
![Immunocytochemistry/Immunofluorescence: Nanog Antibody [NB100-58842] - The stem cell marker (Nanog) staining of iPS-BP cells. Nuclei were stained with DAPI (blue). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0044024) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nanog-Antibody-Immunocytochemistry-Immunofluorescence-NB100-58842-img0015.jpg "Immunocytochemistry/Immunofluorescence: Nanog Antibody [NB100-58842] - The stem cell marker (Nanog) staining of iPS-BP cells. Nuclei were stained with DAPI (blue). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0044024) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: Nanog Antibody [NB100-58842] - Nanog-inducible mouse model. Immunohistochemistry (IHC) for NANOG of paraffin-embedded sections of tail skin from CTR and TG mice treated as indicated in (B). Two magnifications are shown for each tissue (bars correspond to 50 um). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep10205), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nanog-Antibody-Immunohistochemistry-Paraffin-NB100-58842-img0011.jpg)
![Western Blot: Nanog Antibody [NB100-58842] - Whole cell lysate (5, 15, and 50 ug) from F9 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Nanog antibody used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Nanog-Antibody-Western-Blot-NB100-58842-img0009.jpg)
![Immunohistochemistry-Paraffin: Nanog Antibody [NB100-58842] - Nanog-inducible mouse model. Representative hematoxylin and eosin (H&E) staining and immunohistochemistry (IHC) for NANOG, Ki67 and LORICRIN of TPA-treated CTR and TG mice (bars correspond to 50 um). Statistical significance was determined by the two-tailed Student's t test: (*) p < 0.05; (**) p < 0.01. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep10205), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nanog-Antibody-Immunohistochemistry-Paraffin-NB100-58842-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Nanog Antibody [NB100-58842] - Nanog staining in ES cells treatment with leukemia inhibitory factor (LIF). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0044024) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nanog-Antibody-Immunocytochemistry-Immunofluorescence-NB100-58842-img0013.jpg)
![Immunohistochemistry: Nanog Antibody [NB100-58842] - Differentiation of wt and Suv4-20dn teratomas. Representative images of Nanog staining in wt and Suv4-20dn teratomas. Scale bar, 200 um. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0025680) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nanog-Antibody-Immunohistochemistry-NB100-58842-img0016.jpg)
![Immunoprecipitation: Nanog Antibody [NB100-58842] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from F9 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Nanog antibody used for IP at 6 ug per reaction. Nanog was also immunoprecipitated by rabbit anti-Nanog antibody. For blotting immunoprecipitated Nanog, this was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Nanog-Antibody-Immunoprecipitation-NB100-58842-img0010.jpg)
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Nanog Antibody [NB100-58842] - Nanog antibody was tested in DGCR8 knockout Mouse embryonic stem cells (NBA1-19349) with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Nanog-Antibody-Immunocytochemistry-Immunofluorescence-NB100-58842-img0004.jpg)
![c, d Oct4 and Nanog immunodetection in ES cells (c) or ELA cells (d). e Violin plot shows the distribution of green fluorescent protein (GFP) intensity in a TNG-A Nanog::GFP line [16] in LIF/serum (ES cells, red) and 24 h (green) or 48 h (blue) after LIF/serum withdrawal (ELA) or Activin/fibroblast growth factor (FGF)2 induction (EpiSC), respectively. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27154007), licensed under a CC-BY licence.](http://images.novusbio.com/fullsize/nb100-58842_rabbit-polyclonal-nanog-antibody-291220231224351.jpg)
![Western Blot: Nanog Antibody (5A10) [NBP1-04320] - The cell lysates of NIH3T3 (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Nanog (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/Nanog-Antibody-5A10-Western-Blot-NBP1-04320-img0001.jpg "Western Blot: Nanog Antibody (5A10) [NBP1-04320] - The cell lysates of NIH3T3 (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Nanog (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.")
![Immunohistochemistry: Nanog Antibody (5A10) [NBP1-04320] - Nuclear expression of NANOG [(C), red] was demonstrated on the endothelium of the microvessels which expressed CD34 [(C), green] within stroma. The NANOG+ cells [(C), red] that do not express CD34 were seen within the tumor nests and the stroma. Image collected and cropped by CiteAb from the following publication (journal.frontiersin.org/article/10.3389/fsurg.2016.00046), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nanog-Antibody-5A10-Immunohistochemistry-NBP1-04320-img0006.jpg)
![Flow Cytometry: Nanog Antibody (5A10) [NBP1-04320] - Flow cytometry analysis of Nanog in Hep3B cell line, staining at 2-5ug for 1x106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.](http://images.novusbio.com/fullsize/Nanog-Antibody-5A10-Flow-Cytometry-NBP1-04320-img0003.jpg)
![Immunohistochemistry-Paraffin: Nanog Antibody (5A10) [NBP1-04320] - Human seminoma tissue were incubated with anti-human Nanog (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodiumcitrate buffer and detected using Diaminobenzidine (DAB)](http://images.novusbio.com/fullsize/Nanog-Antibody-5A10-Immunohistochemistry-Paraffin-NBP1-04320-img0004.jpg)
![Immunohistochemistry: Nanog Antibody (5A10) [NBP1-04320] - NANOG was seen in cells within the tumor nests [(F), brown, thick arrows] and the stroma [(F), brown, arrowheads], and the endothelium of the microvessels within the stroma [(F), brown, thin arrows]. Image collected and cropped by CiteAb from the following publication (journal.frontiersin.org/article/10.3389/fsurg.2016.00046), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nanog-Antibody-5A10-Immunohistochemistry-NBP1-04320-img0005.jpg)
![Immunohistochemistry-Paraffin: Nanog Antibody [NBP2-13177] - Staining of Nanog in mouse testis using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Nanog-Antibody-Immunohistochemistry-Paraffin-NBP2-13177-img0002.jpg "Immunohistochemistry-Paraffin: Nanog Antibody [NBP2-13177] - Staining of Nanog in mouse testis using DAB with hematoxylin counterstain.")
![Western Blot: Nanog Antibody [NBP2-24941] - Analysis of Nanog in (A) HeLa and (B) NIH 3T3 cell lysate using NBP2-24941 at 2 ug/ml.](http://images.novusbio.com/fullsize/Nanog-Antibody-Western-Blot-NBP2-24941-img0002.jpg "Western Blot: Nanog Antibody [NBP2-24941] - Analysis of Nanog in (A) HeLa and (B) NIH 3T3 cell lysate using NBP2-24941 at 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: Nanog Antibody [NBP2-24941] - Nanog antibody was tested in Ntera2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image objective 40x. An antibody dilution of 1:10 was used.](http://images.novusbio.com/fullsize/Nanog-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24941-img0005.jpg)
![Western Blot: Nanog Antibody [NBP2-24941] - Protein expression of pluripotency markers in uterine cells isolated from control cows and from cows with adenomyosis. MM - molecular weight marker, C - cells obtained from control cows, ADENO - cells obtained from cows with adenomyosis. Image collected and cropped by CiteAb from the following publication (//rbej.biomedcentral.com/articles/10.1186/s12958-015-0106-0), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nanog-Antibody-Western-Blot-NBP2-24941-img0006.jpg)
![Western Blot: Nanog Antibody [NB110-40660] - Staining of Human Ovary lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Nanog-Antibody-Western-Blot-NB110-40660-img0002.jpg "Western Blot: Nanog Antibody [NB110-40660] - Staining of Human Ovary lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Nanog Antibody [NB110-40660] - Staining (green) parts of a colony of induced pluriform stem cells derived from Human Keratinocytes. Antibody at 5 ug/mL. Data kindly provided by CMRB, Center of Regenerative Medicine in Barcelona, Spain.](http://images.novusbio.com/fullsize/Nanog-Antibody-Immunocytochemistry-Immunofluorescence-NB110-40660-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Nanog Antibody (1E6C4) [NBP1-47427] - Confocal immunofluorescence analysis of NTERA-2 cells (left) and HeLa cells (right) using anti-Nanog Mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.](http://images.novusbio.com/fullsize/Nanog-Antibody-1E6C4-Immunocytochemistry-Immunofluorescence-NBP1-47427-img0003.jpg "Immunocytochemistry/Immunofluorescence: Nanog Antibody (1E6C4) [NBP1-47427] - Confocal immunofluorescence analysis of NTERA-2 cells (left) and HeLa cells (right) using anti-Nanog Mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.")
![Western Blot: Nanog Antibody (1E6C4) [NBP1-47427] - Western blot analysis using anti-Nanog monoclonal antibody against NTERA-2 cell lysate (lane 2).](http://images.novusbio.com/fullsize/Nanog-Antibody-1E6C4-Western-Blot-NBP1-47427-img0004.jpg)
![Western Blot: Nanog Antibody (1E6C4) [NBP1-47427] - Whole cell protein from NTERA-2 was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 mg/ml anti-Nanog in 1% milk. Protein was detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Nanog-Antibody-1E6C4-Western-Blot-NBP1-47427-img0005.jpg)
![Western Blot: Nanog Antibody (1E6C4) - Azide and BSA Free [NBP2-80865] - Whole cell protein from NTERA-2 was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 mg/ml anti-Nanog in 1% milk. Protein was detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize2/Nanog-Antibody-(1E6C4)---Azide-and-BSA-Free-Western-Blot-NBP2-80865-img0002.jpg "Western Blot: Nanog Antibody (1E6C4) - Azide and BSA Free [NBP2-80865] - Whole cell protein from NTERA-2 was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 mg/ml anti-Nanog in 1% milk. Protein was detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: Nanog Antibody (1E6C4) - Azide and BSA Free [NBP2-80865] - Confocal immunofluorescence analysis of NTERA-2 cells (left) and HeLa cells (right) using anti-Nanog Mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize2/Nanog-Antibody-(1E6C4)-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80865-img0003.jpg)
![Western Blot: Nanog Antibody (1E6C4) - Azide and BSA Free [NBP2-80865] - Western blot analysis using anti-Nanog monoclonal antibody against NTERA-2 cell lysate (lane 2). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize2/Nanog-Antibody-(1E6C4)---Azide-and-BSA-Free-Western-Blot-NBP2-80865-img0001.jpg)
![Western Blot: Nanog Antibody (23D2-3C6) [NBP2-22632] - Analysis of 60 ug of various whole cell lysates and 10ul of PageRuler Prestained Protein Ladder.](http://images.novusbio.com/fullsize/Nanog-Antibody-23D2-3C6-Western-Blot-NBP2-22632-img0006.jpg "Western Blot: Nanog Antibody (23D2-3C6) [NBP2-22632] - Analysis of 60 ug of various whole cell lysates and 10ul of PageRuler Prestained Protein Ladder.")
![Immunocytochemistry/Immunofluorescence: Nanog Antibody (23D2-3C6) [NBP2-22632] - Analysis of Nanog (green) in H9 embryonic stem cells grown for a few days on Matrigel-coated chamber slides. Cells fixed in 4% paraformaldehyde were permeabilized with 0.1% Triton X-100 for 15 minutes at room temperature. Cells were probed with a Nanog monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBST, and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:100 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI and cells were analyzed by fluorescence microscopy at 20X magnification.](http://images.novusbio.com/fullsize/Nanog-Antibody-23D2-3C6-Immunocytochemistry-Immunofluorescence-NBP2-22632-img0005.jpg)
![Flow Cytometry: Nanog Antibody (23D2-3C6) [NBP2-22632] - Analysis of Nanog (blue histogram) on H9 embryonic stem cells. To generate single cells suspensions, colonies were treated with TrypLE cell dissociation enzyme for 5 minutes at 37C. Cells were incubated with a Nanog monoclonal antibody or mouse IgG (green histogram) at a dilution of 1:100 for 1 hour on ice, washed with PBS + 5% fetal calf serum (FACS buffer), and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:200 for 30 minutes on ice. Cells were washed with cold FACS buffer, resuspended in 500ul of FACS buffer containing 10ul of 4% paraformaldehyde.](http://images.novusbio.com/fullsize/Nanog-Antibody-23D2-3C6-Flow-Cytometry-NBP2-22632-img0002.jpg)
![Western Blot: Nanog Antibody (23D2-3C6) [NBP2-22632] - Analysis of 60 ug of NCCIT nuclear and cytoplasmic fractions lysates and 10ul of PageRuler Prestained Protein Ladder](http://images.novusbio.com/fullsize/Nanog-Antibody-23D2-3C6-Western-Blot-NBP2-22632-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Nanog Antibody (23D2-3C6) [NBP2-22632] - Analysis of Nanog (green) in NTERA-2 and HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a Nanog monoclonal antibody at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody. F-Actin (red) was stained with DyLight-554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/Nanog-Antibody-23D2-3C6-Immunocytochemistry-Immunofluorescence-NBP2-22632-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Nanog Antibody (23D2-3C6) [NBP2-22632] - Analysis of Nanog (green) in HEL 11.4 induced IPS cells grown for a few days on Matrigel-coated chamber slides. Cells fixed in 4% paraformaldehyde were permeabilized with 0.1% Triton X-100 for 15 minutes at room temperature. Cells were probed with a Nanog monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBST, and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:100 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI and cells were analyzed by fluorescence microscopy at 20X magnification.](http://images.novusbio.com/fullsize/Nanog-Antibody-23D2-3C6-Immunocytochemistry-Immunofluorescence-NBP2-22632-img0004.jpg)
![Flow Cytometry: Nanog Antibody (23D2-3C6) [NBP2-22632] - Analysis of Nanog (blue histogram) on HEL 11.4 induced IPS cells. To generate single cells suspensions, colonies were treated with TrypLE cell dissociation enzyme for 5 minutes at 37C. Cells were incubated with a Nanog monoclonal antibody or mouse IgG (green histogram) at a dilution of 1:100 for 1 hour on ice, washed with PBS + 5% fetal calf serum (FACS buffer), and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:200 for 30 minutes on ice. Cells were washed with cold FACS buffer, resuspended in 500ul of FACS buffer containing 10ul of 4% paraformaldehyde.](http://images.novusbio.com/fullsize/Nanog-Antibody-23D2-3C6-Flow-Cytometry-NBP2-22632-img0001.jpg)
![Western Blot: Nanog Antibody (CL5810) [NBP2-61429] - Analysis in human cell line NTERA-2.](http://images.novusbio.com/fullsize/Nanog-Antibody-CL5810-Western-Blot-NBP2-61429-img0006.jpg "Western Blot: Nanog Antibody (CL5810) [NBP2-61429] - Analysis in human cell line NTERA-2.")
![Immunocytochemistry/Immunofluorescence: Nanog Antibody (CL5810) [NBP2-61429] - Staining of NTERA-2 cells using the Anti-NANOG monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/Nanog-Antibody-CL5810-Immunocytochemistry-Immunofluorescence-NBP2-61429-img0001.jpg)
![Immunohistochemistry-Paraffin: Nanog Antibody (CL5810) [NBP2-61429] - Staining of human testis shows absence of positivity in seminiferous ducts as expected (negative control).](http://images.novusbio.com/fullsize/Nanog-Antibody-CL5810-Immunohistochemistry-Paraffin-NBP2-61429-img0004.jpg)
![Immunohistochemistry-Paraffin: Nanog Antibody (CL5810) [NBP2-61429] - Staining of human testis cancer shows strong nuclear immunoreactivity in tumor cells.](http://images.novusbio.com/fullsize/Nanog-Antibody-CL5810-Immunohistochemistry-Paraffin-NBP2-61429-img0002.jpg)
![Immunohistochemistry-Paraffin: Nanog Antibody (CL5810) [NBP2-61429] - Staining of human renal cancer shows absence of positivity in tumor cells as expected (negative control).](http://images.novusbio.com/fullsize/Nanog-Antibody-CL5810-Immunohistochemistry-Paraffin-NBP2-61429-img0003.jpg)
![Western Blot: Nanog Antibody [NBP1-77109] - Human spleen tissue lysate antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/Nanog-Antibody-Western-Blot-NBP1-77109-img0004.jpg "Western Blot: Nanog Antibody [NBP1-77109] - Human spleen tissue lysate antibody at (A) 1 and (B) 2 ug/mL.")
![Immunocytochemistry/Immunofluorescence: Nanog Antibody [NBP1-77109] - Immunofluorescence of NANOG in Human Spleen cells with NANOG antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/Nanog-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77109-img0006.jpg)
![Immunohistochemistry-Paraffin: Nanog Antibody [NBP1-77109] - Human spleen tissue with NANOG antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/Nanog-Antibody-Immunohistochemistry-Paraffin-NBP1-77109-img0002.jpg)