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![Western Blot: IRAK1 Antibody [NBP1-77068] - Species Activity in Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: IRAK NBP1-77068 (1 ug/mL,), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/IRAK1-Antibody-Western-Blot-NBP1-77068-img0009.jpg "Western Blot: IRAK1 Antibody [NBP1-77068] - Species Activity in Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: IRAK NBP1-77068 (1 ug/mL,), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Immunocytochemistry/Immunofluorescence: IRAK1 Antibody [NBP1-77068] - Immunofluorescence Validation of IRAK in Human HeLa Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa Cells labeling IRAK with NBP1-77068 at 20 ug g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/IRAK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77068-img0005.jpg)
![Western Blot: IRAK1 Antibody [NBP1-77068] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: IRAK NBP1-77068 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/IRAK1-Antibody-Western-Blot-NBP1-77068-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: IRAK1 Antibody [NBP1-77068] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines.Loading: 15 ug of lysates per lane.Antibodies: IRAK NBP1-77068 (1 ug/mL), IRAK from Company B (2 ug/mL), beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/IRAK1-Antibody-Western-Blot-NBP1-77068-img0007.jpg)
![<b>Expression of Tagged Proteins Validation. </b>Western Blot: IRAK1 Antibody [NBP1-77068] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human IRAK recombinant protein per lane.Antibodies: IRAK NBP1-77068 (1: 1ug/mL, 2: 2 ug/mL and 3: 4 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/IRAK1-Antibody-Western-Blot-NBP1-77068-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: IRAK1 Antibody [NBP1-77068] - Immunocytochemistry Validation of IRAK in Human HeLa Cells. Immunocytochemical analysis of HeLa cells using anti-IRAK antibody (NBP1-77068) at 10 ug g/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/IRAK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77068-img0002.jpg)
![Western Blot: IRAK1 Antibody (3A9) [H00003654-M02] - Analysis of IRAK1 expression in transfected 293T cell line by IRAK1 monoclonal antibody (M02), clone 3A9.Lane 1: IRAK1 transfected lysate(68 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/IRAK1-Antibody-3A9-Western-Blot-H00003654-M02-img0022.jpg "Western Blot: IRAK1 Antibody (3A9) [H00003654-M02] - Analysis of IRAK1 expression in transfected 293T cell line by IRAK1 monoclonal antibody (M02), clone 3A9.Lane 1: IRAK1 transfected lysate(68 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: IRAK1 Antibody (3A9) [H00003654-M02] - Analysis of monoclonal antibody to IRAK1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/IRAK1-Antibody-3A9-Immunocytochemistry-Immunofluorescence-H00003654-M02-img0015.jpg)
![Immunohistochemistry-Paraffin: IRAK1 Antibody (3A9) [H00003654-M02] - Analysis of monoclonal antibody to IRAK1 on formalin-fixed paraffin-embedded human lung. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/IRAK1-Antibody-3A9-Immunohistochemistry-Paraffin-H00003654-M02-img0017.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: IRAK1 Antibody (3A9) [H00003654-M02] - Analysis of IRAK1 over-expressed 293 cell line, cotransfected with IRAK1 Validated Chimera RNAi ( Cat # H00003654-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with IRAK1 monoclonal antibody (M02) clone 3A9 (Cat # H00003654-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/IRAK1-Antibody-3A9-Western-Blot-H00003654-M02-img0019.jpg)
![Western Blot: IRAK1 Antibody (3A9) [H00003654-M02] - IRAK1 monoclonal antibody (M02), clone 3A9 Analysis of IRAK1 expression in A-431.](http://images.novusbio.com/fullsize/IRAK1-Antibody-3A9-Western-Blot-H00003654-M02-img0021.jpg)
![Immunoprecipitation: IRAK1 Antibody (3A9) [H00003654-M02] - Analysis of IRAK1 transfected lysate using anti-IRAK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with IRAK1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/IRAK1-Antibody-3A9-Immunoprecipitation-H00003654-M02-img0018.jpg)
![ELISA: IRAK1 Antibody (3A9) [H00003654-M02] - Detection limit for recombinant GST tagged IRAK1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/IRAK1-Antibody-3A9-ELISA-H00003654-M02-img0020.jpg)
![Western Blot: IRAK1 Antibody (3F7) [H00003654-M01] - Analysis of IRAK1 expression in transfected 293T cell line by IRAK1 monoclonal antibody (M01), clone 3F7.Lane 1: IRAK1 transfected lysate(68 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/IRAK1-Antibody-3F7-Western-Blot-H00003654-M01-img0015.jpg "Western Blot: IRAK1 Antibody (3F7) [H00003654-M01] - Analysis of IRAK1 expression in transfected 293T cell line by IRAK1 monoclonal antibody (M01), clone 3F7.Lane 1: IRAK1 transfected lysate(68 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: IRAK1 Antibody (3F7) [H00003654-M01] - Analysis of monoclonal antibody to IRAK1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/IRAK1-Antibody-3F7-Immunocytochemistry-Immunofluorescence-H00003654-M01-img0010.jpg)
![Immunohistochemistry-Paraffin: IRAK1 Antibody (3F7) [H00003654-M01] - Analysis of monoclonal antibody to IRAK1 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/IRAK1-Antibody-3F7-Immunohistochemistry-Paraffin-H00003654-M01-img0012.jpg)
![Western Blot: IRAK1 Antibody (3F7) [H00003654-M01] - Western Blot analysis of IRAK1 expression in K-562 ( Cat # L009V1 ).](http://images.novusbio.com/fullsize/IRAK1-Antibody-3F7-Western-Blot-H00003654-M01-img0008.jpg)
![Immunoprecipitation: IRAK1 Antibody (3F7) [H00003654-M01] - Analysis of IRAK1 transfected lysate using anti-IRAK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with IRAK1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/IRAK1-Antibody-3F7-Immunoprecipitation-H00003654-M01-img0013.jpg)
![Sandwich ELISA: IRAK1 Antibody (3F7) [H00003654-M01] - Detection limit for recombinant GST tagged IRAK1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/IRAK1-Antibody-3F7-Sandwich-ELISA-H00003654-M01-img0014.jpg)
![Western Blot: IRAK1 Antibody [NB100-56699] - IRAK Antibody [NB100-56699] - Analysis of IRAK-1 in 1) human HeLa and 2) mouse NIH3T3 lysate using IRAK-1 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/IRAK1-Antibody-Western-Blot-NB100-56699-img0003.jpg "Western Blot: IRAK1 Antibody [NB100-56699] - IRAK Antibody [NB100-56699] - Analysis of IRAK-1 in 1) human HeLa and 2) mouse NIH3T3 lysate using IRAK-1 antibody at 1 ug/ml.")
![Immunocytochemistry/Immunofluorescence: IRAK1 Antibody [NBP2-38726] - Staining of human cell line MCF7 shows localization to nucleus & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/IRAK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38726-img0003.jpg "Immunocytochemistry/Immunofluorescence: IRAK1 Antibody [NBP2-38726] - Staining of human cell line MCF7 shows localization to nucleus & cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: IRAK1 Antibody [NBP2-38726] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glands.](http://images.novusbio.com/fullsize/IRAK1-Antibody-Immunohistochemistry-Paraffin-NBP2-38726-img0004.jpg)
![SDS-Page: Recombinant Human IRAK1 Protein [H00003654-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-IRAK1-Protein-SDS-Page-H00003654-Q01-img0001.jpg "SDS-Page: Recombinant Human IRAK1 Protein [H00003654-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: IRAK1 Antibody (SR2210) [NBP3-21577] - Western blot analysis using NBP3-21577 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-21577_rabbit-irak1-mab-sr2210-6720239533733.jpg "Western Blot: IRAK1 Antibody (SR2210) [NBP3-21577] - Western blot analysis using NBP3-21577 in HeLa cell lysate.")
![Western Blot: IRAK Overexpression Lysate (Adult Normal) [NBP2-11046] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IRAK.](http://images.novusbio.com/fullsize/IRAK-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11046-img0001.jpg "Western Blot: IRAK Overexpression Lysate (Adult Normal) [NBP2-11046] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IRAK.")
![Western Blot: IRAK Overexpression Lysate (Adult Normal) [NBL1-12026] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IRAK.](http://images.novusbio.com/fullsize/IRAK-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12026-img0002.jpg "Western Blot: IRAK Overexpression Lysate (Adult Normal) [NBL1-12026] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IRAK.")