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![Western Blot: HSF1 Antibody [NBP1-97475] - Analysis of HSF1: Lane 1: Molecular Weight Marker, Lane 2: HSF1 (human), (recombinant), Lane 3: HeLa Cell Lysate, Lane 4: HeLa Cell Lysate (Heat Shocked), Lane 5: 3T3 Cell Lysate, Lane 6: PC-12 Cell Lysate, Lane 7: RK-13 Cell Lysate, Lane 8: Vero Cell Lysate.](http://images.novusbio.com/fullsize/HSF1-Antibody-Western-Blot-NBP1-97475-img0002.jpg "Western Blot: HSF1 Antibody [NBP1-97475] - Analysis of HSF1: Lane 1: Molecular Weight Marker, Lane 2: HSF1 (human), (recombinant), Lane 3: HeLa Cell Lysate, Lane 4: HeLa Cell Lysate (Heat Shocked), Lane 5: 3T3 Cell Lysate, Lane 6: PC-12 Cell Lysate, Lane 7: RK-13 Cell Lysate, Lane 8: Vero Cell Lysate.")
![Western Blot: HSF1 Antibody (4B4) [NBP2-42206] - Western Blot analysis of Human Breast adenocarcinoma cell line (MCF7) showing detection of ~65 kDa HSF1 protein using Rat Anti-HSF1 Monoclonal Antibody, Clone 4B4 (NBP2-42206). Lane 1: MW ladder. Lane 2: HSF1 null lysate prepared from mouse embryonic fibroblasts. Lane 3: MCF7 lysate (5 ug). Lane 4: MCF7 lysate (10 ug). Lane 5: MCF7 lysate (20 ug). Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (NBP2-42206) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rat IgG: HRP for 1 hour at RT. Predicted/Observed Size: ~65 kDa. Courtesy of: Dr. Sandro Santagata, Harvard Medical School.](http://images.novusbio.com/fullsize/HSF1-Antibody-4B4-Western-Blot-NBP2-42206-img0004.jpg "Western Blot: HSF1 Antibody (4B4) [NBP2-42206] - Western Blot analysis of Human Breast adenocarcinoma cell line (MCF7) showing detection of ~65 kDa HSF1 protein using Rat Anti-HSF1 Monoclonal Antibody, Clone 4B4 (NBP2-42206). Lane 1: MW ladder. Lane 2: HSF1 null lysate prepared from mouse embryonic fibroblasts. Lane 3: MCF7 lysate (5 ug). Lane 4: MCF7 lysate (10 ug). Lane 5: MCF7 lysate (20 ug). Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (NBP2-42206) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rat IgG: HRP for 1 hour at RT. Predicted/Observed Size: ~65 kDa. Courtesy of: Dr. Sandro Santagata, Harvard Medical School.")
![Immunocytochemistry/Immunofluorescence: HSF1 Antibody (4B4) [NBP2-42206] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Localizes to the nucleus upon activation. Magnification: 100x. Heat Shocked at 42C for 1h.](http://images.novusbio.com/fullsize/HSF1-Antibody-4B4-Immunocytochemistry-Immunofluorescence-NBP2-42206-img0002.jpg)
![Western Blot: HSF1 Antibody (4B4) [NBP2-42206] - analysis of Human A431 and HEK293 cell lysates showing detection of HSF1 protein using Rat Anti-HSF1 Monoclonal Antibody, Clone 4B4 . Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody at 1:1000.](http://images.novusbio.com/fullsize/HSF1-Antibody-4B4-Western-Blot-NBP2-42206-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: HSF1 Antibody (4B4) [NBP2-42206] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: APC Goat Anti-Rat (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Localizes to the nucleus upon activation. Magnification: 20x. Heat Shocked at 42C for 1h.](http://images.novusbio.com/fullsize/HSF1-Antibody-4B4-Immunocytochemistry-Immunofluorescence-NBP2-42206-img0003.jpg)
![Western Blot: HSF1 Antibody (10H4) [NBP2-42205] - Western Blot analysis of Human A431 and HEK293 cell lysates showing detection of HSF1 protein using Rat Anti-HSF1 Monoclonal Antibody, Clone 10H4 (NBP2-42205). Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (NBP2-42205) at 1:1000.](http://images.novusbio.com/fullsize/HSF1-Antibody-10H4-Western-Blot-NBP2-42205-img0004.jpg "Western Blot: HSF1 Antibody (10H4) [NBP2-42205] - Western Blot analysis of Human A431 and HEK293 cell lysates showing detection of HSF1 protein using Rat Anti-HSF1 Monoclonal Antibody, Clone 10H4 (NBP2-42205). Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (NBP2-42205) at 1:1000.")
![Immunocytochemistry/Immunofluorescence: HSF1 Antibody (10H4) [NBP2-42205] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Localizes to the nucleus upon activation. Magnification: 100x. Heat Shocked at 42C for 1h.](http://images.novusbio.com/fullsize/HSF1-Antibody-10H4-Immunocytochemistry-Immunofluorescence-NBP2-42205-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: HSF1 Antibody (10H4) [NBP2-42205] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Rat (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Localizes to the nucleus upon activation. Magnification: 20x. Heat Shocked at 42C for 1h.](http://images.novusbio.com/fullsize/HSF1-Antibody-10H4-Immunocytochemistry-Immunofluorescence-NBP2-42205-img0003.jpg)
![Immunohistochemistry-Paraffin: HSF1 Antibody [NBP1-71931] - Detection of human HSF1 by immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma. Antibody: an affinity purified rabbit anti-HSF1 used at a dilution of 1:5,000 (0.2ug/ml) (left), a different anti-HSF1 antibody used at a dilution of 1:5,000 (0.2ug/ml) (center) and Cat. NBP1-71931 used at a dilution of 1:1,000 (0.2ug/ml) (right). Detection: DAB](http://images.novusbio.com/fullsize/HSF1-Antibody-Immunohistochemistry-Paraffin-NBP1-71931-img0004.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: HSF1 Antibody [NBP1-71931] - Detection of human HSF1 by immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma. Antibody: an affinity purified rabbit anti-HSF1 used at a dilution of 1:5,000 (0.2ug/ml) (left), a different anti-HSF1 antibody used at a dilution of 1:5,000 (0.2ug/ml) (center) and Cat. NBP1-71931 used at a dilution of 1:1,000 (0.2ug/ml) (right). Detection: DAB")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: HSF1 Antibody [NBP1-71931] - Samples: FFPE sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- HSF1 Cat. No. Lot1 used at a dilution of 1:1,000 (1ug/ml) (left), Cat. No. Lot1 used at a dilution of 1:1,000 (1ug/ml) (center) and Cat. No. Lot1 used at a dilution](http://images.novusbio.com/fullsize/HSF1-Antibody-Immunohistochemistry-NBP1-71931-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: HSF1 Antibody [NBP1-71931] - Detection of human HSF1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 ug) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-HSF1 antibody NBP1-71931 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. HSF1 was also immunoprecipitated by two other rabbit anti-HSF1 antibodies, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/HSF1-Antibody-Immunoprecipitation-NBP1-71931-img0003.jpg)
![Western Blot: HSF1 Antibody [NBP1-81665] - Analysis in human cell line PC-3.](http://images.novusbio.com/fullsize/HSF1-Antibody-Western-Blot-NBP1-81665-img0007.jpg "Western Blot: HSF1 Antibody [NBP1-81665] - Analysis in human cell line PC-3.")
![Immunocytochemistry/Immunofluorescence: HSF1 Antibody [NBP1-81665] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/HSF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81665-img0008.jpg)
![Immunohistochemistry-Paraffin: HSF1 Antibody [NBP1-81665] - Staining of human kidney shows strong nuclear and moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/HSF1-Antibody-Immunohistochemistry-Paraffin-NBP1-81665-img0005.jpg)
![Immunohistochemistry-Paraffin: HSF1 Antibody (10H8) [NBP1-97504] - Analysis of human skeletal muscle tissue stained with HSF1, mAb (10H8) at 10ug/ml.](http://images.novusbio.com/fullsize/HSF1-Antibody-10H8-Immunohistochemistry-Paraffin-NBP1-97504-img0002.jpg "Immunohistochemistry-Paraffin: HSF1 Antibody (10H8) [NBP1-97504] - Analysis of human skeletal muscle tissue stained with HSF1, mAb (10H8) at 10ug/ml.")
![Western Blot: HSF1 [p Ser 326] Antibody (SR2246) [NBP3-21520] - Western blot analysis using NBP3-21520 in HeLa cell lysates treated with heat.](http://images.novusbio.com/fullsize/nbp3-21520_rabbit-hsf1-p-ser-326-mab-sr2246-57202316112194.jpg "Western Blot: HSF1 [p Ser 326] Antibody (SR2246) [NBP3-21520] - Western blot analysis using NBP3-21520 in HeLa cell lysates treated with heat.")
![Immunocytochemistry/Immunofluorescence: HSF1 Antibody (009) [NBP2-90081] - Staining of Human HSF1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human HSF1 monoclonal antibody (1:60) at 4? overnight. Then cells were stained with the Alexa Fluor® 549-conjugated Goat Anti-rabbit IgG secondary antibody (red).](http://images.novusbio.com/fullsize/HSF1-Antibody-009-Immunocytochemistry-Immunofluorescence-NBP2-90081-img0001.jpg "Immunocytochemistry/Immunofluorescence: HSF1 Antibody (009) [NBP2-90081] - Staining of Human HSF1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human HSF1 monoclonal antibody (1:60) at 4? overnight. Then cells were stained with the Alexa Fluor® 549-conjugated Goat Anti-rabbit IgG secondary antibody (red).")
![Immunohistochemistry-Paraffin: HSF1 Antibody (009) [NBP2-90081] - Staining of human HSF1 in human tonsil with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/HSF1-Antibody-009-Immunohistochemistry-Paraffin-NBP2-90081-img0003.jpg)
![Immunohistochemistry-Paraffin: HSF1 Antibody (009) [NBP2-90081] - Staining of human HSF1 in human placenta with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/HSF1-Antibody-009-Immunohistochemistry-Paraffin-NBP2-90081-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: HSF1 Antibody (1A11) [H00003297-M09] - Analysis of monoclonal antibody to HSF1 on HeLa cell. Antibody concentration 20 ug/ml](http://images.novusbio.com/fullsize/HSF1-Antibody-1A11-Immunocytochemistry-Immunofluorescence-H00003297-M09-img0006.jpg "Immunocytochemistry/Immunofluorescence: HSF1 Antibody (1A11) [H00003297-M09] - Analysis of monoclonal antibody to HSF1 on HeLa cell. Antibody concentration 20 ug/ml")
![Sandwich ELISA: HSF1 Antibody (1A11) [H00003297-M09] - Detection limit for recombinant GST tagged HSF1 is 3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HSF1-Antibody-1A11-Sandwich-ELISA-H00003297-M09-img0005.jpg)
![Western Blot: HSF1 [p Ser326] Antibody (2A7) [NBP3-26417] - Positive Western Blot detected in Hela whole cell lysate. All lanes: HSF1 [p Ser326] Antibody at 0.8ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 82 KDa Observed band size: 82 KDa](http://images.novusbio.com/fullsize/nbp3-26417_rabbit-hsf1-p-ser-326-mab-2a7-282202411485839.jpg "Western Blot: HSF1 [p Ser326] Antibody (2A7) [NBP3-26417] - Positive Western Blot detected in Hela whole cell lysate. All lanes: HSF1 [p Ser326] Antibody at 0.8ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 82 KDa Observed band size: 82 KDa")
![Immunocytochemistry/Immunofluorescence: HSF1 [p Ser326] Antibody (2A7) [NBP3-26417] - Analysis of HSF1 [p Ser326] Antibody (2A7) diluted at 1:80 and staining in Hela cells. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26417_rabbit-hsf1-p-ser-326-mab-2a7-282202412475132.jpg)