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![Knockout Validated: GCLM Antibody [NBP1-33405] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with GCLM antibody [N1N3]. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GCLM-Antibody-Western-Blot-NBP1-33405-img0008.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: GCLM Antibody [NBP1-33405] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with GCLM antibody [N1N3]. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: GCLM Antibody [NBP1-33405] - Sample (30 ug of whole cell lysate) A: H1299 12% SDS PAGE; antibody diluted at 1:5000.](http://images.novusbio.com/fullsize/GCLM-Antibody-Western-Blot-NBP1-33405-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: GCLM Antibody [NBP1-33405] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/GCLM-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33405-img0004.jpg)
![Immunohistochemistry-Paraffin: GCLM Antibody [NBP1-33405] - BT474 xenograft, using GCLM antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/GCLM-Antibody-Immunohistochemistry-Paraffin-NBP1-33405-img0005.jpg)
![Western Blot: GCLM Antibody (2B8) [H00002730-M01] - GCLM monoclonal antibody (M01), clone 2B8 Analysis of GCLM expression in HeLa.](http://images.novusbio.com/fullsize/GCLM-Antibody-2B8-Western-Blot-H00002730-M01-img0004.jpg "Western Blot: GCLM Antibody (2B8) [H00002730-M01] - GCLM monoclonal antibody (M01), clone 2B8 Analysis of GCLM expression in HeLa.")
![Sandwich ELISA: GCLM Antibody (2B8) [H00002730-M01] - Detection limit for recombinant GST tagged GCLM is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/GCLM-Antibody-2B8-Sandwich-ELISA-H00002730-M01-img0003.jpg)
![Western Blot: GCLM Antibody [NBP2-16624] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with GCLM antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301 was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GCLM-Antibody-Western-Blot-NBP2-16624-img0009.jpg "Western Blot: GCLM Antibody [NBP2-16624] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with GCLM antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301 was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: GCLM Antibody [NBP2-16624] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/GCLM-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16624-img0004.jpg)
![Western Blot: GCLM Antibody [NBP2-16624] - Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/GCLM-Antibody-Western-Blot-NBP2-16624-img0001.jpg)
![Western Blot: GCLM Antibody [NBP2-16624] - Sample (50 ug of whole cell lysate) A: rat liver, 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/GCLM-Antibody-Western-Blot-NBP2-16624-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: GCLM Antibody [NBP2-16624] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with GCLM antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GCLM-Antibody-Western-Blot-NBP2-16624-img0005.jpg)
![Western Blot: GCLM Antibody [NBP2-16624] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with GCLM antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GCLM-Antibody-Western-Blot-NBP2-16624-img0007.jpg)
![Western Blot: GCLM Antibody [NBP1-83361] - Analysis in human cell line U-251 MG and human cell line MCF-7.](http://images.novusbio.com/fullsize/GCLM-Antibody-Western-Blot-NBP1-83361-img0010.jpg "Western Blot: GCLM Antibody [NBP1-83361] - Analysis in human cell line U-251 MG and human cell line MCF-7.")
![Immunocytochemistry/Immunofluorescence: GCLM Antibody [NBP1-83361] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/GCLM-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83361-img0008.jpg)
![Immunohistochemistry-Paraffin: GCLM Antibody [NBP1-83361] - Staining of human thyroid gland shows strong cytoplasmic positivity with a granular pattern in glandular cells.](http://images.novusbio.com/fullsize/GCLM-Antibody-Immunohistochemistry-Paraffin-NBP1-83361-img0009.jpg)
![Western Blot: GCLM Antibody (CPTC-GCLM-1) [NBP3-07298] - Western Blot Analysis of A549, A431 & HepG2 cell lysates using GCLM Mouse Monoclonal Antibody (CPTC-GCLM-1).](http://images.novusbio.com/fullsize/GCLM-Antibody-CPTC-GCLM-1-Western-Blot-NBP3-07298-img0003.jpg "Western Blot: GCLM Antibody (CPTC-GCLM-1) [NBP3-07298] - Western Blot Analysis of A549, A431 & HepG2 cell lysates using GCLM Mouse Monoclonal Antibody (CPTC-GCLM-1).")
![Immunohistochemistry-Paraffin: GCLM Antibody (CPTC-GCLM-1) [NBP3-07298] - Formalin-fixed, paraffin-embedded human tonsil stained with GCLM Mouse Monoclonal Antibody (CPTC-GCLM-1).](http://images.novusbio.com/fullsize/GCLM-Antibody-CPTC-GCLM-1-Immunohistochemistry-Paraffin-NBP3-07298-img0002.jpg)
![Immunohistochemistry-Paraffin: GCLM Antibody (CPTC-GCLM-1) [NBP3-07298] - Formalin-fixed, paraffin-embedded human tonsil stained with GCLM Mouse Monoclonal Antibody (CPTC-GCLM-1).](http://images.novusbio.com/fullsize/GCLM-Antibody-CPTC-GCLM-1-Immunohistochemistry-Paraffin-NBP3-07298-img0001.jpg)
![Western Blot: GCLM Overexpression Lysate (Adult Normal) [NBL1-11012] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GCLM.](http://images.novusbio.com/fullsize/GCLM-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11012-img0002.jpg "Western Blot: GCLM Overexpression Lysate (Adult Normal) [NBL1-11012] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GCLM.")
![ELISA: Mouse GCLM ELISA Kit (Colorimetric) [NBP2-67984] - Samples were spiked with high concentrations of Mouse GCLM and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-67984_mouse-gclm-elisa-kit-colorimetric-1322024169226.jpg "ELISA: Mouse GCLM ELISA Kit (Colorimetric) [NBP2-67984] - Samples were spiked with high concentrations of Mouse GCLM and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse GCLM ELISA Kit (Colorimetric) [NBP2-67984] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-67984_mouse-gclm-elisa-kit-colorimetric-4320249173486.jpg)
![ELISA: Human GCLM ELISA Kit (Colorimetric) [NBP2-67983] - Samples were spiked with high concentrations of Human GCLM and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-67983_human-gclm-elisa-kit-colorimetric-13220241614303.jpg "ELISA: Human GCLM ELISA Kit (Colorimetric) [NBP2-67983] - Samples were spiked with high concentrations of Human GCLM and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human GCLM ELISA Kit (Colorimetric) [NBP2-67983] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-67983_human-gclm-elisa-kit-colorimetric-4320248595167.jpg)
![ELISA: Rat GCLM ELISA Kit (Colorimetric) [NBP2-67985] - Samples were spiked with high concentrations of Rat GCLM and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-67985_rat-gclm-elisa-kit-colorimetric-13220241626175.jpg "ELISA: Rat GCLM ELISA Kit (Colorimetric) [NBP2-67985] - Samples were spiked with high concentrations of Rat GCLM and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat GCLM ELISA Kit (Colorimetric) [NBP2-67985] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-67985_rat-gclm-elisa-kit-colorimetric-4320249163353.jpg)
![Western Blot: GCLM Antibody (0U3J2) [NBP3-15380] - Western blot analysis of extracts of various cell lines, using GCLM antibody (NBP3-15380) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/GCLM-Antibody-0U3J2-Western-Blot-NBP3-15380-img0001.jpg "Western Blot: GCLM Antibody (0U3J2) [NBP3-15380] - Western blot analysis of extracts of various cell lines, using GCLM antibody (NBP3-15380) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
