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![Knockdown Validated: Fascin Antibody [NBP1-91887] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Fascin knockout (KO) HeLa cell line. PVDF membrane was probed with 0.4 ug/ml of Rabbit Anti-Human Fascin Polyclonal Antibody (Catalog # NBP1-91887) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Fascin at approximately 55 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Fascin-Antibody-Knockdown-Validated-NBP1-91887-img0021.jpg "<b>Genetic Strategies Validation. </b>Knockdown Validated: Fascin Antibody [NBP1-91887] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Fascin knockout (KO) HeLa cell line. PVDF membrane was probed with 0.4 ug/ml of Rabbit Anti-Human Fascin Polyclonal Antibody (Catalog # NBP1-91887) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Fascin at approximately 55 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: Fascin Antibody [NBP1-91887] - Analysis in human cell lines PC-3 and SK-MEL-30 using anti-FSCN1 antibody. Corresponding FSCN1 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/Fascin-Antibody-Western-Blot-NBP1-91887-img0014.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Fascin Antibody [NBP1-91887] - Staining of human cerebellum, cerebral cortex, testis and tonsil using Anti-Fascin antibody NBP1-91887 (A) shows similar protein distribution across tissues to independent antibody NBP2-58042 (B).](http://images.novusbio.com/fullsize/Fascin-Antibody-Immunohistochemistry-Paraffin-NBP1-91887-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Fascin Antibody [NBP1-91887] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Fascin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91887-img0010.jpg)
![Immunohistochemistry-Paraffin: Fascin Antibody [NBP1-91887] - Staining of human tonsil shows strong cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/Fascin-Antibody-Immunohistochemistry-NBP1-91887-img0020.jpg)
![Western Blot: Fascin Antibody [NBP1-91887] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Fascin-Antibody-Western-Blot-NBP1-91887-img0008.jpg)
![Immunohistochemistry-Paraffin: Fascin Antibody [NBP1-91887] - Staining of human cerebellum shows strong cytoplasmic positivity in cells in molecular layer.](http://images.novusbio.com/fullsize/Fascin-Antibody-Immunohistochemistry-Paraffin-NBP1-91887-img0015.jpg)
![Immunohistochemistry-Paraffin: Fascin Antibody [NBP1-91887] - Staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.](http://images.novusbio.com/fullsize/Fascin-Antibody-Immunohistochemistry-Paraffin-NBP1-91887-img0017.jpg)
![Immunohistochemistry-Paraffin: Fascin Antibody [NBP1-91887] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Fascin-Antibody-Immunohistochemistry-Paraffin-NBP1-91887-img0019.jpg)
![Western Blot: Fascin Antibody [NBP3-03708] - Analysis of extracts of various cell lines, using Fascin antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.](http://images.novusbio.com/fullsize/Fascin-Antibody-Western-Blot-NBP3-03708-img0004.jpg "Western Blot: Fascin Antibody [NBP3-03708] - Analysis of extracts of various cell lines, using Fascin antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.")
![Immunocytochemistry/Immunofluorescence: Fascin Antibody [NBP3-03708] - Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] Fascin/Fascin Rabbit pAb (NBP3-03708) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Fascin-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03708-img0006.jpg)
![Immunohistochemistry-Paraffin: Fascin Antibody [NBP3-03708] - Immunohistochemistry of paraffin-embedded mouse testis using [KO Validated] Fascin/Fascin Rabbit pAb (NBP3-03708) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Fascin-Antibody-Immunohistochemistry-Paraffin-NBP3-03708-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Fascin Antibody [NBP3-03708] - Immunofluorescence analysis of C6 cells using [KO Validated] Fascin/Fascin Rabbit pAb (NBP3-03708) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Fascin-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03708-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: Fascin Antibody [NBP3-03708] - Analysis of extracts from normal (control) and FSCN1 knockout (KO) HeLa cells, using Fascin antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Fascin-Antibody-Knockout-Validated-NBP3-03708-img0003.jpg)
![Immunohistochemistry-Paraffin: Fascin Antibody [NBP2-58042] - Staining of human tonsil shows strong cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/Fascin-Antibody-Immunohistochemistry-Paraffin-NBP2-58042-img0009.jpg "Immunohistochemistry-Paraffin: Fascin Antibody [NBP2-58042] - Staining of human tonsil shows strong cytoplasmic positivity in non-germinal center cells.")
![Immunohistochemistry-Paraffin: Fascin Antibody [NBP2-58042] - Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in cells in molecular layer.](http://images.novusbio.com/fullsize/Fascin-Antibody-Immunohistochemistry-Paraffin-NBP2-58042-img0001.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Fascin Antibody [NBP2-58042] - Staining in human cerebral cortex and colon tissues using anti-FSCN1 antibody. Corresponding FSCN1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Fascin-Antibody-Immunohistochemistry-Paraffin-NBP2-58042-img0004.jpg)
![Immunohistochemistry-Paraffin: Fascin Antibody [NBP2-58042] - Staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.](http://images.novusbio.com/fullsize/Fascin-Antibody-Immunohistochemistry-Paraffin-NBP2-58042-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Fascin Antibody [NBP2-58042] - Staining of human cerebellum, cerebral cortex, testis and tonsil using Anti-Fascin antibody NBP2-58042 (A) shows similar protein distribution across tissues to independent antibody NBP1-91887 (B).](http://images.novusbio.com/fullsize/Fascin-Antibody-Immunohistochemistry-Paraffin-NBP2-58042-img0007.jpg)
![Immunohistochemistry-Paraffin: Fascin Antibody [NBP2-58042] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Fascin-Antibody-Immunohistochemistry-Paraffin-NBP2-58042-img0008.jpg)
![Western Blot: Fascin Antibody (1B3V6) [NBP3-16828] - Western blot analysis of extracts of various cell lines, using Fascin Rabbit mAb (NBP3-16828) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Fascin-Antibody-1B3V6-Western-Blot-NBP3-16828-img0004.jpg "Western Blot: Fascin Antibody (1B3V6) [NBP3-16828] - Western blot analysis of extracts of various cell lines, using Fascin Rabbit mAb (NBP3-16828) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: Fascin Antibody (1B3V6) [NBP3-16828] - Immunohistochemistry of paraffin-embedded mouse spleen using Fascin Rabbit mAb (NBP3-16828) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Fascin-Antibody-1B3V6-Immunohistochemistry-Paraffin-NBP3-16828-img0003.jpg)
![Immunohistochemistry-Paraffin: Fascin Antibody (1B3V6) [NBP3-16828] - Immunohistochemistry of paraffin-embedded rat spleen using Fascin Rabbit mAb (NBP3-16828) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Fascin-Antibody-1B3V6-Immunohistochemistry-Paraffin-NBP3-16828-img0001.jpg)
![Immunohistochemistry-Paraffin: Fascin Antibody (1B3V6) [NBP3-16828] - Immunohistochemistry of paraffin-embedded human esophageal cancer using Fascin Rabbit mAb (NBP3-16828) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Fascin-Antibody-1B3V6-Immunohistochemistry-Paraffin-NBP3-16828-img0002.jpg)
![Flow Cytometry: Fascin Antibody (SAIC-32C-205) - Azide and BSA Free [NBP3-20100] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-32C-205 (NBP3-20100, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20100_rabbit-fascin-mab-saic-32c-205-azide-and-bsa-free-2742023924291.png "Flow Cytometry: Fascin Antibody (SAIC-32C-205) - Azide and BSA Free [NBP3-20100] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-32C-205 (NBP3-20100, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.")
![Immunocytochemistry/Immunofluorescence: Fascin Antibody (SAIC-32C-205) - Azide and BSA Free [NBP3-20100] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-32C-205 (NBP3-20100) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20100, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20100_rabbit-fascin-mab-saic-32c-205-azide-and-bsa-free-2742023924234.png)