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![Western Blot: EEF2K Antibody [NBP3-23302] - Western blot image of EEF2K in human A431 (lane 1), Jurkat (lane 2), and HeLa (lane 3) cells, and immunoprecipitation of EEF2K from HeLa cell lysate using anti-EEF2K (C-terminus) antibody (lanes 4 & 6). Negative control immunopreciptations with no antibody are shown in lane 5 and 7. The blots were probed with anit-EEF2K (C-terminus) (lanes 1-5) or with anti-EEF2K (N-terminal) antibody (lanes 6 & 7).](http://images.novusbio.com/fullsize/nbp3-23302_rabbit-eef2k-pab-19920231856952.jpg "Western Blot: EEF2K Antibody [NBP3-23302] - Western blot image of EEF2K in human A431 (lane 1), Jurkat (lane 2), and HeLa (lane 3) cells, and immunoprecipitation of EEF2K from HeLa cell lysate using anti-EEF2K (C-terminus) antibody (lanes 4 & 6). Negative control immunopreciptations with no antibody are shown in lane 5 and 7. The blots were probed with anit-EEF2K (C-terminus) (lanes 1-5) or with anti-EEF2K (N-terminal) antibody (lanes 6 & 7).")
![Immunocytochemistry/Immunofluorescence: EEF2K Antibody [NBP3-23302] - Immunocytochemical labeling of EEF2K in paraformaldehyde fixed and NP-40 permeabilized A431 cells. The cells were labeled with rabbit polyclonal anti-EEF2K (NBP3-23302). The antibody was detected using goat anti-rabbit DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23302_rabbit-eef2k-pab-199202318202919.jpg)
![Western Blot: EEF2K Antibody [NB100-87019] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-87019 used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. eEF2 Kinase was successfully immunoprecipitated by NB100-87018, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/EEF2K-Antibody-Western-Blot-NB100-87019-img0002.jpg "Western Blot: EEF2K Antibody [NB100-87019] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-87019 used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. eEF2 Kinase was successfully immunoprecipitated by NB100-87018, which recognizes an upstream epitope.")
![Immunohistochemistry-Paraffin: EEF2K Antibody [NB100-87019] - Section of human stomach carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-eEF2 Kinase used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/EEF2K-Antibody-Immunohistochemistry-Paraffin-NB100-87019-img0004.jpg)
![Western Blot: EEF2K Antibody [NBP2-92950] - Analysis of extracts of various cell lines, using EEF2K at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 40s.](http://images.novusbio.com/fullsize/EEF2K-Antibody-Western-Blot-NBP2-92950-img0003.jpg "Western Blot: EEF2K Antibody [NBP2-92950] - Analysis of extracts of various cell lines, using EEF2K at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 40s.")
![Immunocytochemistry/Immunofluorescence: EEF2K Antibody [NBP2-92950] - Analysis of A-549 cells using EEF2K . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/EEF2K-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92950-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: EEF2K Antibody [NBP2-92950] - Analysis of extracts from normal (control) and EEF2K knockout (KO) HeLa cells, using EEF2K antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:Basic ECL Kit](http://images.novusbio.com/fullsize/EEF2K-Antibody-Knockout-Validated-NBP2-92950-img0004.jpg)
![Western Blot: EEF2K Antibody [H00029904-B01P] - Analysis of EEF2K expression in human colon.](http://images.novusbio.com/fullsize/EEF2K-Antibody-Western-Blot-H00029904-B01P-img0002.jpg "Western Blot: EEF2K Antibody [H00029904-B01P] - Analysis of EEF2K expression in human colon.")
![Western Blot: EEF2K Antibody [H00029904-B01P] - Analysis of EEF2K expression in HepG2.](http://images.novusbio.com/fullsize/EEF2K-Antibody-Western-Blot-H00029904-B01P-img0001.jpg)
![Western Blot: EEF2K Antibody [H00029904-B01P] - Analysis of EEF2K expression in transfected 293T cell line by EEF2K polyclonal antibody. Lane 1: EEF2K transfected lysate(79.75 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/EEF2K-Antibody-Western-Blot-H00029904-B01P-img0003.jpg)
![Western Blot: EEF2K [p Ser445] Antibody [NBP3-23311] - Western blot image of human Jurkat cells treated with calyculin A (100 nM) for 30 min. (lanes 1-6), then some lanes of the blot were treated with lambda phosphatase (lanes 2, 4 & 6). The blot was probed with rabbit polyclonals anti-EEF2K (C-terminus) (lanes 1 & 2), anti-EEF2K (Ser-445) phospho-specific (lanes 3 & 4), and anti-EEF2K (Ser-398) phospho-specific (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23311_rabbit-eef2k-p-ser445-pab-19920231410147.jpg "Western Blot: EEF2K [p Ser445] Antibody [NBP3-23311] - Western blot image of human Jurkat cells treated with calyculin A (100 nM) for 30 min. (lanes 1-6), then some lanes of the blot were treated with lambda phosphatase (lanes 2, 4 & 6). The blot was probed with rabbit polyclonals anti-EEF2K (C-terminus) (lanes 1 & 2), anti-EEF2K (Ser-445) phospho-specific (lanes 3 & 4), and anti-EEF2K (Ser-398) phospho-specific (lanes 5 & 6).")
![Western Blot: EEF2K [p Ser500] Antibody [NBP3-23314] - Western blot image of C2C12 untreated (lanes 1 & 3) or treated with calyculin A (100 nM) for 30 min. (lanes 2 & 4). The blot was probed with anti-EEF2K (N-terminus) (lanes 1 & 2) and anti-EEF2K (Ser-500) (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23314_rabbit-eef2k-p-ser500-pab-189202317355210.jpg "Western Blot: EEF2K [p Ser500] Antibody [NBP3-23314] - Western blot image of C2C12 untreated (lanes 1 & 3) or treated with calyculin A (100 nM) for 30 min. (lanes 2 & 4). The blot was probed with anti-EEF2K (N-terminus) (lanes 1 & 2) and anti-EEF2K (Ser-500) (lanes 3 & 4).")
![Western Blot: EEF2K [p Ser78] Antibody [NBP3-23304] - Western blot image of C2C12 treated with calyculin A (100 nM) for 30 min. (lanes 1 & 3), then the blot was treated with lambda phosphatase (lanes 2 & 4). The blot was probed with anti-EEF2K (N-terminus) at 1:500 (lanes 1 & 2) and anti-EEF2K (Ser-78) (NBP3-23304) at 1:1000.](http://images.novusbio.com/fullsize/nbp3-23304_rabbit-eef2k-p-ser78-pab-19920231992815.jpg "Western Blot: EEF2K [p Ser78] Antibody [NBP3-23304] - Western blot image of C2C12 treated with calyculin A (100 nM) for 30 min. (lanes 1 & 3), then the blot was treated with lambda phosphatase (lanes 2 & 4). The blot was probed with anti-EEF2K (N-terminus) at 1:500 (lanes 1 & 2) and anti-EEF2K (Ser-78) (NBP3-23304) at 1:1000.")
![Western Blot: EEF2K [p Ser398] Antibody [NBP3-23310] - Western blot image of human Jurkat cells treated with calyculin A (100 nM) for 30 min. (lanes 1-6), then some lanes of the blot were treated with lambda phosphatase (lanes 2, 4 & 6). The blot was probed with rabbit polyclonals anti-EEF2K (C-terminus) (lanes 1 & 2), anti-EEF2K (Ser-445) phospho-specific (lanes 3 & 4), and anti-EEF2K (Ser-398) phospho-specific (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23310_rabbit-eef2k-p-ser398-pab-19920231856936.jpg "Western Blot: EEF2K [p Ser398] Antibody [NBP3-23310] - Western blot image of human Jurkat cells treated with calyculin A (100 nM) for 30 min. (lanes 1-6), then some lanes of the blot were treated with lambda phosphatase (lanes 2, 4 & 6). The blot was probed with rabbit polyclonals anti-EEF2K (C-terminus) (lanes 1 & 2), anti-EEF2K (Ser-445) phospho-specific (lanes 3 & 4), and anti-EEF2K (Ser-398) phospho-specific (lanes 5 & 6).")
![Western Blot: EEF2K Antibody [NBP3-12694] - Lane A: A431 Whole Cell LysateLane B: H1299 Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:82 kDa](http://images.novusbio.com/fullsize/EEF2K-Antibody-Western-Blot-NBP3-12694-img0001.jpg "Western Blot: EEF2K Antibody [NBP3-12694] - Lane A: A431 Whole Cell LysateLane B: H1299 Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:82 kDa")
![Immunoprecipitation: EEF2K Antibody [NBP3-12694] - Lane A:0.5 mg HeLa Whole Cell Lysate4 uL anti-EEF2K rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-EEF2K rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 82 kDaObserved band size :100 kDa](http://images.novusbio.com/fullsize/EEF2K-Antibody-Immunoprecipitation-NBP3-12694-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: EEF2K Antibody [NBP2-58790] - Staining of human cell line HeLa shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/EEF2K-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58790-img0001.jpg "Immunocytochemistry/Immunofluorescence: EEF2K Antibody [NBP2-58790] - Staining of human cell line HeLa shows localization to nucleoplasm.")