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![Western Blot: Doublecortin Antibody [NBP1-72042] - staining of Mouse fetal Brain lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Doublecortin-Antibody-Western-Blot-NBP1-72042-img0001.jpg "Western Blot: Doublecortin Antibody [NBP1-72042] - staining of Mouse fetal Brain lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Doublecortin Antibody [NBP1-72042] - Immunofluorescence analysis of paraformaldehyde fixed KNRK cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Doublecortin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-72042-img0007.jpg)
![Immunohistochemistry-Paraffin: Doublecortin Antibody [NBP1-72042] - Negative Control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.](http://images.novusbio.com/fullsize/Doublecortin-Antibody-Immunohistochemistry-Paraffin-NBP1-72042-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Doublecortin Antibody [NBP1-72042] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL).](http://images.novusbio.com/fullsize/Doublecortin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-72042-img0005.jpg)
![Immunohistochemistry-Paraffin: Doublecortin Antibody [NBP1-72042] - Staining of paraffin embedded Human Cerebellum. Antibody at 2 ug/mL. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/Doublecortin-Antibody-Immunohistochemistry-Paraffin-NBP1-72042-img0003.jpg)
![Western Blot: Doublecortin Antibody (3E1) [NBP1-92684] - Analysis of rat whole brain lysates using mouse mAb to Doublecortin (DCX), NBP1-92684, dilution 1:1,000 in green: [1] protein standard (red), [2] adult rat brain, [3] embryonic E20 rat brain. Strong bands at 40, 45kDa correspond to DCX protein, detected only in the developing brain.](http://images.novusbio.com/fullsize/Doublecortin-Antibody-3E1-Western-Blot-NBP1-92684-img0005.jpg "Western Blot: Doublecortin Antibody (3E1) [NBP1-92684] - Analysis of rat whole brain lysates using mouse mAb to Doublecortin (DCX), NBP1-92684, dilution 1:1,000 in green: [1] protein standard (red), [2] adult rat brain, [3] embryonic E20 rat brain. Strong bands at 40, 45kDa correspond to DCX protein, detected only in the developing brain.")
![Immunocytochemistry/Immunofluorescence: Doublecortin Antibody (3E1) [NBP1-92684] - Analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to Doublecortin (DCX), NBP1-92684, dilution 1:1,000 in red, and costained with chicken pAb to Microtubule Associated Protein2 (MAP2), dilution 1:10,000 in green. The blue is DAPI staining of nuclear DNA. DCX antibody reveals strong cytoplasmic staining in a population of small developing neurons and their processes, while MAP2 antibody stains dendrites and perikarya of mature neurons. Therefore DCX antibody is an excellent marker of developing neuronal cells.](http://images.novusbio.com/fullsize/Doublecortin-Antibody-3E1-Immunocytochemistry-Immunofluorescence-NBP1-92684-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Doublecortin Antibody (3E1) [NBP1-92684] - Rat brain neural cultures stained with NBP1-92684 (green), a chicken polyclonal antibody to GFAP (NB300-213, red) and DNA (blue). The NBP1-92684 antibody reveals strong cytoplasmic staining in a population of small developing neurons. These cells are often found in small clumps as in these cultures, as in this example. Note that they are not positive for MAP2, which is characteristic of more mature neurons, and that the mature neuron shown is negative for NBP1-92684. This Doublecortin antibody is therefore an excellent marker of developing neuronal cells.](http://images.novusbio.com/fullsize/Doublecortin-Antibody-3E1-Immunocytochemistry-Immunofluorescence-NBP1-92684-img0003.jpg)
![Western Blot: Doublecortin Antibody (2G5) [NBP2-37476] - Analysis using DCX mouse mAb against Mouse heart (1) lysate.](http://images.novusbio.com/fullsize/Doublecortin-Antibody-2G5-Western-Blot-NBP2-37476-img0003.jpg "Western Blot: Doublecortin Antibody (2G5) [NBP2-37476] - Analysis using DCX mouse mAb against Mouse heart (1) lysate.")
![Immunocytochemistry/Immunofluorescence: Doublecortin Antibody (2G5) [NBP2-37476] - Immunofluorescence analysis of HepG2 cells using DCX mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/Doublecortin-Antibody-2G5-Immunocytochemistry-Immunofluorescence-NBP2-37476-img0004.jpg)
![Immunohistochemistry-Paraffin: Doublecortin Antibody (2G5) [NBP2-37476] - Analysis of kidney cancer tissues using DCX mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Doublecortin-Antibody-2G5-Immunohistochemistry-NBP2-37476-img0007.jpg)
![Flow Cytometry: Doublecortin Antibody (2G5) [NBP2-37476] - Analysis of SK-N-SH cells using DCX mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Doublecortin-Antibody-2G5-Flow-Cytometry-NBP2-37476-img0005.jpg)
![Western Blot: Doublecortin Antibody (2G5) [NBP2-37476] - Analysis using DCX mAb against human DCX recombinant protein. (Expected MW is 34.1 kDa)](http://images.novusbio.com/fullsize/Doublecortin-Antibody-2G5-Western-Blot-NBP2-37476-img0002.jpg)
![Immunohistochemistry-Paraffin: Doublecortin Antibody (2G5) [NBP2-37476] - Analysis of brain tissue tissues using DCX mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Doublecortin-Antibody-2G5-Immunohistochemistry-NBP2-37476-img0006.jpg)
![ELISA: Doublecortin Antibody (2G5) [NBP2-37476] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Doublecortin-Antibody-2G5-ELISA-NBP2-37476-img0001.jpg)
![Western Blot: Doublecortin Antibody (BLR168J) [NBP3-14742] - Rabbit anti-Doublecortin recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Doublecortin-Antibody-BLR168J-Western-Blot-NBP3-14742-img0004.jpg "Western Blot: Doublecortin Antibody (BLR168J) [NBP3-14742] - Rabbit anti-Doublecortin recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. chemiluminescence with an exposure time of 30 seconds.")
![Immunocytochemistry: Doublecortin Antibody (BLR168J) [NBP3-14742] - Rabbit anti-Doublecortin recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG Substrate DAB](http://images.novusbio.com/fullsize/Doublecortin-Antibody-BLR168J-Immunocytochemistry-NBP3-14742-img0003.jpg)
![Immunohistochemistry-Paraffin: Doublecortin Antibody (BLR168J) [NBP3-14742] - Rabbit anti-Doublecortin recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate DAB](http://images.novusbio.com/fullsize/Doublecortin-Antibody-BLR168J-Immunohistochemistry-Paraffin-NBP3-14742-img0002.jpg)
![Immunohistochemistry-Paraffin: Doublecortin Antibody (BLR168J) [NBP3-14742] - Rabbit anti-Doublecortin recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate DAB](http://images.novusbio.com/fullsize/Doublecortin-Antibody-BLR168J-Immunohistochemistry-Paraffin-NBP3-14742-img0001.jpg)
![Immunoprecipitation- Doublecortin Antibody (BLR168J)-NBP3-14742-Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from SK-N-BE(2) cells prepared using NETN lysis buffer. Antibodies: Rabbit antiDoublecortin recombinant monoclonal antibody [BLR168J] used for IP at 20 ul/mg lysate. Doublecortin was also immunoprecipitated by a second antibody against a different epitope of Doublecortin (BL-1974A-2B1). For blotting immunoprecipitated Doublecortin was used at 1:1000. Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-14742_rabbit-doublecortin-mab-immunoprecipitation-2102023105019..jpg)
![Immunocytochemistry/Immunofluorescence: Doublecortin Antibody [NBP2-69992] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Doublecortin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-69992-img0002.jpg "Immunocytochemistry/Immunofluorescence: Doublecortin Antibody [NBP2-69992] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Immunohistochemistry-Paraffin: Doublecortin Antibody [NBP2-69992] - (6ug/ml) staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/NBP2-69992_Immunohistochemistry-Paraffin-Doublecortin-Antibody-NBP2-69992-6ug-ml-staining-of-paraffin-embedded-Human-Cortex-Heat-induced-antigen-retrieval-with-citrate-buffer-pH-6-HRP-staining--29320239254027.jpg)
![Immunohistochemistry-Paraffin: Doublecortin Antibody [NBP2-69992] - Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.](http://images.novusbio.com/fullsize/NBP2-69992_Immunohistochemistry-Paraffin-Doublecortin-Antibody-NBP2-69992-Negative-Control-showing-staining-of-paraffin-embedded-Human-Cortex-with-no-primary-antibody--29320239254010.jpg)
![Flow Cytometry: Doublecortin Antibody [NBP2-69992] - Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Doublecortin-Antibody-Flow-Cytometry-NBP2-69992-img0001.jpg)
![Western Blot: Doublecortin Antibody (1G12) [H00001641-M01] - Analysis of DCX expression in transfected 293T cell line by DCX monoclonal antibody (M01), clone 1G12.Lane 1: DCX transfected lysate(41.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Doublecortin-Antibody-1G12-Western-Blot-H00001641-M01-img0005.jpg "Western Blot: Doublecortin Antibody (1G12) [H00001641-M01] - Analysis of DCX expression in transfected 293T cell line by DCX monoclonal antibody (M01), clone 1G12.Lane 1: DCX transfected lysate(41.4 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Doublecortin Antibody (1G12) [H00001641-M01] - Analysis of monoclonal antibody to DCX on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Doublecortin-Antibody-1G12-Immunocytochemistry-Immunofluorescence-H00001641-M01-img0004.jpg)
![Western Blot: Doublecortin Antibody (4A3) [NBP2-03081] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Doublecortin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Doublecortin.](http://images.novusbio.com/fullsize/Doublecortin-Antibody-4A3-Western-Blot-NBP2-03081-img0002.jpg "Western Blot: Doublecortin Antibody (4A3) [NBP2-03081] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Doublecortin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Doublecortin.")
![Immunocytochemistry/Immunofluorescence: Doublecortin Antibody (4A3) [NBP2-03081] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Doublecortin.](http://images.novusbio.com/fullsize/Doublecortin-Antibody-4A3-Immunocytochemistry-Immunofluorescence-NBP2-03081-img0003.jpg)
![Flow Cytometry: Doublecortin Antibody (4A3) [NBP2-03081] - Analysis of Jurkat cells, using anti-Doublecortin antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Doublecortin-Antibody-4A3-Flow-Cytometry-NBP2-03081-img0001.jpg)
![Western Blot: Doublecortin Antibody [NBP1-32662] - Non-transfected (-) and Doublecortin-transfected (+, ) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Doublecortin antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/Doublecortin-Antibody-Western-Blot-NBP1-32662-img0004.jpg "Western Blot: Doublecortin Antibody [NBP1-32662] - Non-transfected (-) and Doublecortin-transfected (+, ) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Doublecortin antibody at a dilution of 1:1000.")
![Immunocytochemistry/Immunofluorescence: Doublecortin Antibody [NBP1-32662] - Immunofluorescent staining of neurons derived from human ESC with a 1:200 dilution of GeneTex anti-doublecortin antibody. Anti-doublecortin antibody (red) colocalizes with anti-MAP2 antibody (green). Image courtesy of Jente Lu, UC Irvine.](http://images.novusbio.com/fullsize/Doublecortin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32662-img0005.jpg)
![Western Blot: Doublecortin Antibody (6H1B3) [NBP3-15628] - Western blot analysis of extracts of various cell lines, using Doublecortin antibody (NBP3-15628) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Doublecortin-Antibody-6H1B3-Western-Blot-NBP3-15628-img0001.jpg "Western Blot: Doublecortin Antibody (6H1B3) [NBP3-15628] - Western blot analysis of extracts of various cell lines, using Doublecortin antibody (NBP3-15628) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: Doublecortin Antibody [NBP2-55074] - Staining of human cell line SH-SY5Y shows localization to cytosol.](http://images.novusbio.com/fullsize/Doublecortin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55074-img0001.jpg "Immunocytochemistry/Immunofluorescence: Doublecortin Antibody [NBP2-55074] - Staining of human cell line SH-SY5Y shows localization to cytosol.")
![Western Blot: Doublecortin Overexpression Lysate (Adult Normal) [NBL1-09760] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Doublecortin.](http://images.novusbio.com/fullsize/Doublecortin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09760-img0002.jpg "Western Blot: Doublecortin Overexpression Lysate (Adult Normal) [NBL1-09760] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Doublecortin.")