| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Purified | NBP3-25349-0.025mg | |||
| Purified | NBP3-25349-0.05mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Purified | NBP3-25350SS | |||
| Purified | NBP3-25350 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Agarose | NBP1-97138 | |||
| Biotin | NBP1-96836 | |||
| FITC | NBP1-96884 | |||
| HRP | NBP1-96964 | |||
| Rhodamine | NBP1-96885 | |||
| Texas Red | NBP1-96835 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-96964 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-96889 | |||
| FITC | NBP1-96848 | |||
| HRP | NBP1-97075 | |||
| Texas Red | NBP1-96794 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Agarose | NBP1-96830 | |||
| Alkaline Phosphatase | NBP1-97219 | |||
| Biotin | NBP1-96855 | |||
| FITC | NBP1-96880 | |||
| HRP | NBP1-97066 | |||
| Rhodamine | NBP1-96882 | |||
| Texas Red | NBP1-96854 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-96891 | |||
| FITC | NBP1-96847 | |||
| HRP | NBP1-97077 | |||
| Rhodamine | NBP1-96850 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Agarose | NBP1-97145 | |||
| Alkaline Phosphatase | NBP1-97183 | |||
| Biotin | NBP1-96930 | |||
| FITC | NBP1-96866 | |||
| HRP | NBP1-97082 | |||
| Rhodamine | NBP1-96867 | |||
| Texas Red | NBP1-96931 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-97183 | |||
![Simple Western: Mre11 Antibody [NB100-142] - Image shows a specific band for Mre11 in 0.5 mg/mL of HeLa lysate. A band appears at the theoretical molecular weight of 81 kDa for this product. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Mre11-Antibody-Simple-Western-NB100-142-img0010.jpg "Simple Western: Mre11 Antibody [NB100-142] - Image shows a specific band for Mre11 in 0.5 mg/mL of HeLa lysate. A band appears at the theoretical molecular weight of 81 kDa for this product. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Immunocytochemistry/Immunofluorescence: Mre11 Antibody [NB100-142] - IF analysis of Mre11 on HeLa. Image from verified customer review.](http://images.novusbio.com/fullsize/Mre11-Antibody-Immunocytochemistry-Immunofluorescence-NB100-142-img0009.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: Mre11 Antibody [NB100-142] - Mre11 and NBS1 contribute to OriP replication and EBV episome stability. D98/HR1 cells were transfected with siRNA for Mre11 or NBS1, or Luciferase control and assayed by immunoblot (IB) with antibodies specific for Mre11 (left pane) or NBS1 (right panel), or PCNA (lower panel) as a loading control. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosone/article?id=10.1371/journal.pone.0001257) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Mre11-Antibody-Knockdown-Validated-NB100-142-img0012.jpg)
![Immunohistochemistry: Mre11 Antibody [NB100-142] - Immunohistochemical staining of MRE11 in a human epidermis cross section.](http://images.novusbio.com/fullsize/Mre11-Antibody-Immunohistochemistry-NB100-142-img0004.jpg)
![Western Blot: Mre11 Antibody [NB100-142] - Western blot analysis of Mre11 on 50 ug of HeLa and MEF lysates, displaying bands at the molecular weight of 81 kDa.](http://images.novusbio.com/fullsize/Mre11-Antibody-Western-Blot-NB100-142-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Mre11 Antibody [NB100-142] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Mre11 [NB100-142] at a 1:500 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Mre11-Antibody-Immunocytochemistry-Immunofluorescence-NB100-142-img0011.jpg)
![Chromatin Immunoprecipitation: Mre11 Antibody [NB100-142] - Aggregated END-seq signal and MRE11 ChIP-seq RPM in WT (left) and Atm -/- (right). To fairly compare ChIP-seq signal between WT and Atm -/-, MRE11 scale is proportional to spike-in normalized END-seq RPM for each genotype. Individual hotspot examples (chr12:34,592,264-34,598,265) are shown below. Note that decreased MRE11 coverage is observed within NDR of Atm -/-. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32051414/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Mre11 Antibody-Chromatin Immunoprecipitation-NB100-142-img0013.jpg)
![Knockdown Validated: Mre11 Antibody [NB100-142] - TIG-1 human primary fibroblasts, whole cell lysate (30 ug). Image from verified customer review.](http://images.novusbio.com/fullsize/Mre11-Antibody-Knockdown-Validated-NB100-142-img0008.jpg)
![Chromatin Immunoprecipitation: ARNT/HIF-1 beta Antibody [NB100-110] - ChIP analysis with ARNT/HIF-1 beta antibody [NB100-110]. Allele specific HIF-binding at the rs12814794 associated enhancer. B) Allele-specific qPCR for rs12814794 from DNA fragments isolated in HIF-ChIP experiments (HIF-1A and HIF-1B) or input DNA from primary renal tubular cells (PTC, n = 4 individuals). DNA from individuals homozygous for the AA or GG genotype were used as positive controls. C) Quantification of the two different alleles (A or G) at rs12814794 in the ChIP-seq reads from HIF-1A and HIF-1B immunoprecipitations (PTC #1 Fig 3). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pgen.1006872) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ARNT-HIF-1-beta-Antibody-Chromatin-Immunoprecipitation-NB100-110-img0007.jpg "Chromatin Immunoprecipitation: ARNT/HIF-1 beta Antibody [NB100-110] - ChIP analysis with ARNT/HIF-1 beta antibody [NB100-110]. Allele specific HIF-binding at the rs12814794 associated enhancer. B) Allele-specific qPCR for rs12814794 from DNA fragments isolated in HIF-ChIP experiments (HIF-1A and HIF-1B) or input DNA from primary renal tubular cells (PTC, n = 4 individuals). DNA from individuals homozygous for the AA or GG genotype were used as positive controls. C) Quantification of the two different alleles (A or G) at rs12814794 in the ChIP-seq reads from HIF-1A and HIF-1B immunoprecipitations (PTC #1 Fig 3). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pgen.1006872) licensed under a CC-BY license.")
![Western Blot: ARNT/HIF-1 beta Antibody [NB100-110] - Analysis of HIF-1 beta using ARNT/HIF-1 beta antibody [NB100-110] in MCF7 whole cell lysate. Theoretical molecular weight is 86.6 kDa. Observed molecular weight ~95 kDa.](http://images.novusbio.com/fullsize/ARNT-HIF-1-beta-Antibody-Western-Blot-NB100-110-img0001.jpg)
![Immunohistochemistry: ARNT/HIF-1 beta Antibody [NB100-110] - Immunohistochemical staining of human skin epidermis with ARNT/HIF-1 beta antibody [NB100-110].](http://images.novusbio.com/fullsize/ARNT-HIF-1-beta-Antibody-Immunohistochemistry-NB100-110-img0004.jpg)
![Chromatin Immunoprecipitation: ARNT/HIF-1 beta Antibody [NB100-110] - ChIP analysis with ARNT/HIF-1 beta antibody [NB100-110]. MDA-MB-231 cells were exposed to 20% or 1% O2 for 16 hours, and chromatin immunoprecipitation (ChIP) was performed with the indicated antibody (Ab). Primers flanking the HIF binding site were used for qPCR. ChIP image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ARNT-HIF-1-beta-Antibody-Chromatin-Immunoprecipitation-NB100-110-img0009.jpg)
![Chromatin Immunoprecipitation (ChIP): ARNT/HIF-1 beta Antibody [NB100-110] - ChIP analysis using ARNT/HIF-1 beta antibody [NB100-110]. MCF7 were exposed to 20% or 1% O2 for 16h, and ChIP assays were performed using IgG or antibodies against HIF-1a, HIF-2a, HIF-1b. ChIP image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ARNT-HIF-1-beta-Antibody-Chromatin-Immunoprecipitation-ChIP-NB100-110-img0006.jpg)
![Chromatin Immunoprecipitation: ARNT/HIF-1 beta Antibody [NB100-110] - ChIP analysis with ARNT/HIF-1 beta antibody [NB100-110]. A scatter plot showing HIF-1B ChIP-seq signal at the same set of binding sites exhibiting a comparable increase in HIF-1B binding in cells expressing transfected HIF-2A. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0134645) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ARNT-HIF-1-beta-Antibody-Chromatin-Immunoprecipitation-NB100-110-img0008.jpg)
![Simple Western: ARNT/HIF-1 beta Antibody [NB100-110] - Simple Western analysis using ARNT/HIF-1 beta antibody [NB100-110]. Lane view shows a specific band for HIF-1 beta in 0.5 mg/ml of Hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Theoretical molecular weight is 86.6 kDa. Observed molecular weight ~106 kDa.](http://images.novusbio.com/fullsize/ARNT-HIF-1-beta-Antibody-Simple-Western-NB100-110-img0005.jpg)
![Western Blot: Rabbit IgG Isotype Control [NBP2-36463] - Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule (NBP2-36463). Lane 2: Rabbit IgG F(ab) Fragment (NBP1-97016). Lane 3: Rabbit IgG F(c) Fragment (NBP1-96784). Lane 4: Rabbit IgM Whole Molecule (NBP1-96968). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Blocked in blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins. (NBP1-72732) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.](http://images.novusbio.com/fullsize/Rabbit-IgG-Isotype-Control-Western-Blot-NBP2-36463-img0028.jpg "Western Blot: Rabbit IgG Isotype Control [NBP2-36463] - Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule (NBP2-36463). Lane 2: Rabbit IgG F(ab) Fragment (NBP1-97016). Lane 3: Rabbit IgG F(c) Fragment (NBP1-96784). Lane 4: Rabbit IgM Whole Molecule (NBP1-96968). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Blocked in blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins. (NBP1-72732) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.")
![Flow (Intracellular): Rabbit IgG Isotype Control [NBP2-36463] - Intracellular staining for GLI1, GLI2 and GLI3 in untreated human B cell lines (BCWM.1, MWCL-1 and RPCI-WM1) using a secondary anti-rabbit IgG PE conjugated antibody. Image courtesy of Sherine Elsawa, Northern Illinois University.](http://images.novusbio.com/fullsize/Rabbit-IgG-Isotype-Control-Flow-Intracellular-NBP2-36463-img0007.jpg)
![SDS-Page: Rabbit IgG Isotype Control [NBP2-36463] - Lane 1: Non-reduced Rabbit IgG Whole Molecule. Lane 2: 5uL OPAL Pre-stained Marker. Lane 3: Reduced Rabbit IgG Whole Molecule. Load: 1ug per lane. Predicted/Observed size: Non-reduced at 150-170 kDa , Reduced at 55, 25 kDa.](http://images.novusbio.com/fullsize/Rabbit-IgG-Isotype-Control-SDS-Page-NBP2-36463-img0018.jpg)
![SDS-Page: Rabbit IgG Isotype Control [NBP2-36463] - SDS-Page of Rabbit IgG. Lane 1: Rabbit IgG - Non-Reduced. Lane 2: Rabbit IgG - Reduced. Load: 1.0 ug per lane. Predicted/Observed Size: Non-reduced - 130 kDa, Reduced - 55 and 28 kDa for Rabbit IgG. Other Band(s): None.](http://images.novusbio.com/fullsize/Rabbit IgG Isotype Control-SDS-Page-NBP2-36463-img0030.jpg)
![SDS-Page: Rabbit IgG Isotype Control [NBP2-36463] - Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rabbit IgG Whole Molecule. Lane 2: Reduced Rabbit IgG F(ab) Fragment. Lane 3: Reduced Rabbit IgG F(c) Fragment. Lane 4: Reduced Rabbit IgM Whole Molecule. Load: 1 ug for F(ab) and F(c); 1.2 ug for IgG and IgM. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.](http://images.novusbio.com/fullsize/Rabbit-IgG-Isotype-Control-SDS-Page-NBP2-36463-img0029.jpg)
![Immunocytochemistry/Immunofluorescence: Guinea Pig IgG Isotype Control [NBP1-97036] - Guinea Pig and Mouse IgG control. Image collected and cropped by CiteAb from the following publication (mdpi.com/2073-4409/8/11/1369), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Guinea-Pig-IgG-Isotype-Control-Immunocytochemistry-Immunofluorescence-NBP1-97036-img0002.jpg "Immunocytochemistry/Immunofluorescence: Guinea Pig IgG Isotype Control [NBP1-97036] - Guinea Pig and Mouse IgG control. Image collected and cropped by CiteAb from the following publication (mdpi.com/2073-4409/8/11/1369), licensed under a CC-BY license.")
![SDS-Page: Guinea Pig IgG Isotype Control [NBP1-97036] - Lane 1: Guinea Pig IgG - Non-reduced. Lane 2: Guinea Pig IgG - Reduced. Load: 1.0 ug per lane. Predicted/Observed size: 25 & 55 kDa - Reduced, 160 kDa - Non-reduced for IgG whole molecule. Other band(s): None.](http://images.novusbio.com/fullsize/Guinea-Pig-IgG-Isotype-Control-SDS-Page-NBP1-97036-img0001.jpg)
![Immunoprecipitation: Mouse IgG2a Isotype Control (M2A) [NBP1-96778] - Cell lysates from T24 cells treated for 3h with vehicle or perifosine (0.5, 1 or 2.5 uM) were immunoprecipitated (IP) using a monoclonal anti-CB Ab. The immunoprecipitates were separated via 12% SDS-PAGE and then immunoblotted (IB) using Abs against phospho-tyrosine (pTyr) and CB. The lysates were also immunoprecipitated using isotype-matched IgG, which was used as a control. Image collected and cropped by CiteAb from the following publication (impactjournals.com/oncoscience/index.php?abs=147), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Mouse-IgG2a-Isotype-Control-M2A-Immunoprecipitation-NBP1-96778-img0001.jpg "Immunoprecipitation: Mouse IgG2a Isotype Control (M2A) [NBP1-96778] - Cell lysates from T24 cells treated for 3h with vehicle or perifosine (0.5, 1 or 2.5 uM) were immunoprecipitated (IP) using a monoclonal anti-CB Ab. The immunoprecipitates were separated via 12% SDS-PAGE and then immunoblotted (IB) using Abs against phospho-tyrosine (pTyr) and CB. The lysates were also immunoprecipitated using isotype-matched IgG, which was used as a control. Image collected and cropped by CiteAb from the following publication (impactjournals.com/oncoscience/index.php?abs=147), licensed under a CC-BY license.")
![Immunoprecipitation: Mouse IgG2a Isotype Control (M2A) [NBP1-96778] - Cell lysates from T24 BC cells either untreated or treated with sorafenib (20 uM) for 1 or 3 h were immunoprecipitated (IP) using a monoclonal anti-CB Ab. The immunoprecipitates were separated via 12% SDS-PAGE and then immunoblotted (IB) using Abs against phospho-tyrosine (anti-pTyr) and CB. The lysates were also immunoprecipitated using isotype-matched IgG, which was used as a control. Image collected and cropped by CiteAb from the following publication (impactjournals.com/oncoscience/index.php?abs=147), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Mouse-IgG2a-Isotype-Control-M2A-Immunoprecipitation-NBP1-96778-img0002.jpg)
![SDS-Page Results of Mouse IgG2a Isotype Control (M2A).Lane 1: Mouse IgG2a Isotype Control (M2A) Reduced [1.0ug]. Lane 2: Opal Prestained Molecular Weight Marker](http://images.novusbio.com/fullsize/nbp1-96778_mouse-monoclonal-mouse-igg2a-isotype-control-m2a-23520231821370.jpg)
![Flow Cytometry: Mouse IgG2b Kappa Isotype Control (M2BK) [NBP1-96980] - Cells were stained with the positive antibody (right) and Isotype control (left) and unstained cells for 1h at 4C. Image from verified customer review.](http://images.novusbio.com/fullsize/Mouse-IgG2b-Kappa-Isotype-Control-M2BK-NBP1-96980-img0001.jpg "Flow Cytometry: Mouse IgG2b Kappa Isotype Control (M2BK) [NBP1-96980] - Cells were stained with the positive antibody (right) and Isotype control (left) and unstained cells for 1h at 4C. Image from verified customer review.")
![Western Blot: Human IgG Isotype Control [NBP1-97043] - SDS-PAGE of Human IgG.Lane 1: Reduced Human IgG.Lane 2: 5uL OPAL Pre-stained MarkerLane 3: Non-reduced Human IgG.Load: 1ug per lane.Predicted/Observed size: Non-reduced at 180-245 kDa, Reduced at 55, 25 kDa.](http://images.novusbio.com/fullsize/Human-IgG-Isotype-Control-Western-Blot-NBP1-97043-img0003.jpg "Western Blot: Human IgG Isotype Control [NBP1-97043] - SDS-PAGE of Human IgG.Lane 1: Reduced Human IgG.Lane 2: 5uL OPAL Pre-stained MarkerLane 3: Non-reduced Human IgG.Load: 1ug per lane.Predicted/Observed size: Non-reduced at 180-245 kDa, Reduced at 55, 25 kDa.")
![SDS-Page: Human IgG Isotype Control [NBP1-97043] - SDS-PAGE of Human IgG Isotype control.Lane 1: Reduced Human IgG Isotype control.Lane 2: 5uL OPAL Pre-stained Marker. Load: 1ug per lane.Predicted/Observed size: Non-reduced at 180-245 kDa , Reduced at 55, 25 kDa.](http://images.novusbio.com/fullsize/Human IgG Isotype Control-SDS-Page-NBP1-97043-img0004.jpg)
![Western Blot: Rabbit IgG Isotype Control [NBP1-97041] - Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule (NBP2-36463). Lane 2: Rabbit IgG F(ab) Fragment (NBP1-97016). Lane 3: Rabbit IgG F(c) Fragment (NBP1-96784). Lane 4: Rabbit IgM Whole Molecule (NBP1-96968). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Blocked in blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins. (NBP1-72732) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.](http://images.novusbio.com/fullsize/Rabbit-IgG-Isotype-Control-Western-Blot-NBP1-97041-img0006.jpg "Western Blot: Rabbit IgG Isotype Control [NBP1-97041] - Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule (NBP2-36463). Lane 2: Rabbit IgG F(ab) Fragment (NBP1-97016). Lane 3: Rabbit IgG F(c) Fragment (NBP1-96784). Lane 4: Rabbit IgM Whole Molecule (NBP1-96968). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Blocked in blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins. (NBP1-72732) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.")
![Western Blot: Rabbit IgG Isotype Control [NBP1-97041]](http://images.novusbio.com/fullsize/Rabbit-IgG-Isotype-Control-Western-Blot-NBP1-97041-img0002.jpg)
![SDS-Page: Rabbit IgG Isotype Control [NBP1-97041] - Lane 1: Rabbit IgG - Non-Reduced. Lane 2: Rabbit IgG - Reduced. Load: 1.0 ug per lane. Predicted/Observed Size: Non-reduced - 130 kDa, Reduced - 55 and 28 kDa for Rabbit IgG.](http://images.novusbio.com/fullsize/Rabbit-IgG-Isotype-Control-SDS-Page-NBP1-97041-img0001.jpg)
![SDS-Page: Rabbit IgG Isotype Control [NBP1-97041] - SDS-PAGE of Rabbit IgG Isotype control. Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rabbit IgG Isotype control. Lane 2: Reduced Rabbit IgG F(ab) Fragment. Lane 3: Reduced Rabbit IgG F(c) Fragment. Lane 4: Reduced Rabbit IgM Whole Molecule. Load: 1 ug for F(ab) and F(c); 1.2 ug for IgG and IgM. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.](http://images.novusbio.com/fullsize/Rabbit IgG Isotype Control-SDS-Page-NBP1-97041-img0008.jpg)
![SDS-Page: Rabbit IgG Isotype Control [NBP1-97041] - SDS-PAGE of Rabbit IgG Isotype control. Lane 1: Non-reduced Rabbit IgG Isotype control. Lane 2: 5uL OPAL Pre-stained Marker. Lane 3: Reduced Rabbit IgG Isotype control. Load: 1ug per lane. Predicted/Observed size: Non-reduced at 150-170 kDa , Reduced at 55, 25 kDa.](http://images.novusbio.com/fullsize/Rabbit-IgG-Isotype-Control-SDS-Page-NBP1-97041-img0007.jpg)
![Western Blot: Mouse IgG3 Isotype Control (MG3) [NBP1-96777] - SDS-PAGE of Mouse IgG3. Lane 1: Non-Reduced Human IgG. Lane 2: 5uL OPAL Pre-stained Marker MB-210-0500. Lane 3: Reduced Mouse IgG3. Load: 1ug per lane. Predicted/Observed size: Non-reduced at 180-245 kDa, Reduced at 55, 25 kDa.](http://images.novusbio.com/fullsize/Mouse-IgG3-Isotype-Control-MG3-Western-Blot-NBP1-96777-img0003.jpg "Western Blot: Mouse IgG3 Isotype Control (MG3) [NBP1-96777] - SDS-PAGE of Mouse IgG3. Lane 1: Non-Reduced Human IgG. Lane 2: 5uL OPAL Pre-stained Marker MB-210-0500. Lane 3: Reduced Mouse IgG3. Load: 1ug per lane. Predicted/Observed size: Non-reduced at 180-245 kDa, Reduced at 55, 25 kDa.")
![Dot Blot: Mouse IgG3 Isotype Control (MG3) [NBP1-96777] - A three-fold serial dilution of Fluorescein conjugated Mouse IgG3 subclass starting at 200ug was spotted onto 0.45 um nitrocellulose. Image from the FITC version of this antibody.](http://images.novusbio.com/fullsize/Mouse-IgG3-Isotype-Control-MG3-Dot-Blot-NBP1-96777-img0002.jpg)
![SDS-Page: Mouse IgM Lambda Isotype Control (MML) [NBP1-96976] - SDS-PAGE of Mouse IgM Lambda isotype control (38). Lane 1: 5 uL Opal Prestained Marker. Lane 2: Reduced Mouse IgG Whole Molecule. Lane 3: Reduced Mouse F(c) Fragment. Lane 4: Reduced Mouse F(ab) Fragment. Lane 5: Mouse IgM Lambda isotype control (38). Load: 1 ug per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab') at 25 kDa; IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.](http://images.novusbio.com/fullsize/Mouse-IgM-Lambda-Isotype-Control-MML-SDS-Page-NBP1-96976-img0002.jpg "SDS-Page: Mouse IgM Lambda Isotype Control (MML) [NBP1-96976] - SDS-PAGE of Mouse IgM Lambda isotype control (38). Lane 1: 5 uL Opal Prestained Marker. Lane 2: Reduced Mouse IgG Whole Molecule. Lane 3: Reduced Mouse F(c) Fragment. Lane 4: Reduced Mouse F(ab) Fragment. Lane 5: Mouse IgM Lambda isotype control (38). Load: 1 ug per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab') at 25 kDa; IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.")
![SDS-Page: Mouse IgM Lambda Isotype Control (MML) [NBP1-96976] - SDS-PAGE of Mouse IgM lambda isotype control. Lane 1: 1x Reduced Mouse IgM lambda. Lane 2: Opal Prestained Standard. Lane 3: 1X Non-reduced Mouse IgM lambda. 4-20% Lonza SDS-PAGE; Coomassie Stained; ChemiDoc Imaged.](http://images.novusbio.com/fullsize/Mouse-IgM-Lambda-Isotype-Control-MML-SDS-Page-NBP1-96976-img0004.jpg)
![Western Blot: Rabbit IgM Isotype Control [NBP1-96968] - Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule (NBP2-36463). Lane 2: Rabbit IgG F(ab) Fragment (NBP1-97016). Lane 3: Rabbit IgG F(c) Fragment (NBP1-96784). Lane 4: Rabbit IgM Whole Molecule (NBP1-96968). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Blocked in blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins. (NBP1-72732) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.](http://images.novusbio.com/fullsize/Rabbit IgM Isotype Control-Western Blot-NBP1-96968-img0003.jpg "Western Blot: Rabbit IgM Isotype Control [NBP1-96968] - Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule (NBP2-36463). Lane 2: Rabbit IgG F(ab) Fragment (NBP1-97016). Lane 3: Rabbit IgG F(c) Fragment (NBP1-96784). Lane 4: Rabbit IgM Whole Molecule (NBP1-96968). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Blocked in blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins. (NBP1-72732) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.")
![SDS-Page: Rabbit IgM Isotype Control [NBP1-96968] - Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rabbit IgG Whole Molecule. Lane 2: Reduced Rabbit IgG F(ab) Fragment. Lane 3: Reduced Rabbit IgG F(c) Fragment. Lane 4: Reduced Rabbit IgM Whole Molecule. Load: 1 ug for F(ab) and F(c); 1.2 ug for IgG and IgM.](http://images.novusbio.com/fullsize/Rabbit-IgM-Isotype-Control-SDS-Page-NBP1-96968-img0001.jpg)
![Western Blot: Rat IgG Isotype Control [NBP1-97040] - SDS-Page: Rat IgG Isotype Control [Agarose Immobilized] [NBP1-97145] - Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rat IgG Whole Molecule Agarose Conjugated. Lane 2: Reduced Rat IgG F(c) Fragment. Lane 3: Reduced Rat IgG Fab Fragment. Lane 4: Reduced Rat IgM Whole Molecule. Load: 1 ug of IgG, F(c), Fab; 1.5 ug of IgM. Image using the Agarose form of this antibody.](http://images.novusbio.com/fullsize/Rat-IgG-Isotype-Control-Western-Blot-NBP1-97040-img0002.jpg "Western Blot: Rat IgG Isotype Control [NBP1-97040] - SDS-Page: Rat IgG Isotype Control [Agarose Immobilized] [NBP1-97145] - Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rat IgG Whole Molecule Agarose Conjugated. Lane 2: Reduced Rat IgG F(c) Fragment. Lane 3: Reduced Rat IgG Fab Fragment. Lane 4: Reduced Rat IgM Whole Molecule. Load: 1 ug of IgG, F(c), Fab; 1.5 ug of IgM. Image using the Agarose form of this antibody.")
![SDS-Page: Rat IgG Isotype Control [NBP1-97040] - SDS PAGE of Rat IgG Whole Molecule. Lane 1: Non-Reduced Rat IgG Whole Molecule. Lane 2: 5 uL Opal Prestained Marker. Lane 3: Reduced Rat IgG Whole Molecule. Load: 1 ug per lane.](http://images.novusbio.com/fullsize/Rat IgG Isotype Control-SDS-Page-NBP1-97040-img0003.jpg)