| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Atto594 | NBP3-14765-100ug | |||
| Atto594 | NBP3-14765-200ug | |||
| Atto594 | NBP3-14765-500ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1338-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB13381-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB13382-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB13383-SP | |||
![Immunohistochemistry-Frozen: alpha-Synuclein Antibody [NBP2-15365] - Frozen-sectioned adult mouse hippocampus. Green: alpha Synuclein protein stained by alpha Synuclein antibody diluted at 1:250. Red: NeuN, stained by NeuN antibody [2Q158] diluted at 1:500.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-Immunohistochemistry-Frozen-NBP2-15365-img0024.jpg "Immunohistochemistry-Frozen: alpha-Synuclein Antibody [NBP2-15365] - Frozen-sectioned adult mouse hippocampus. Green: alpha Synuclein protein stained by alpha Synuclein antibody diluted at 1:250. Red: NeuN, stained by NeuN antibody [2Q158] diluted at 1:500.")
![<b>Independent Antibodies Validation. </b>Western Blot: alpha-Synuclein Antibody [NBP2-15365] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membranes were blotted with alpha Synuclein antibody diluted at 1:2000 and competitor's antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-Western-Blot-NBP2-15365-img0027.jpg)
![Immunohistochemistry-Frozen: alpha-Synuclein Antibody [NBP2-15365] - Frozen-sectioned adult mouse cerebellum. Green: alpha Synuclein protein stained by alpha Synuclein antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559 diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-Immunohistochemistry-Frozen-NBP2-15365-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody [NBP2-15365] - DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Synuclein protein stained by alpha Synuclein antibody diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [1338] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15365-img0026.jpg)
![Immunohistochemistry-Frozen: alpha-Synuclein Antibody [NBP2-15365] - Frozen sectioned E13.5 Rat brain. Green: alpha Synuclein protein stained by alpha Synuclein antibody diluted at 1:250. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-Immunohistochemistry-Frozen-NBP2-15365-img0016.jpg)
![Immunohistochemistry-Paraffin: alpha-Synuclein Antibody [NBP2-15365] - Rat brain. alpha Synuclein antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Synuclein-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-15365-img0009.jpg)
![Western Blot: alpha-Synuclein Antibody [NBP2-15365] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-Western-Blot-NBP2-15365-img0032.jpg)
![Immunohistochemistry-Paraffin: alpha-Synuclein Antibody [NBP2-15365] - Mouse hippocampus. alpha Synuclein stained by alpha Synuclein antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-Immunohistochemistry-Paraffin-NBP2-15365-img0033.jpg)
![Western Blot: alpha-Synuclein Antibody [NBP2-15365] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-Western-Blot-NBP2-15365-img0022.jpg)
![Immunohistochemistry-Paraffin: alpha-Synuclein Antibody [NBP2-15365] - Human colon carcinoma, using alpha Synuclein antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Synuclein-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-15365-img0006.jpg)
![Immunohistochemistry-Paraffin: alpha-Synuclein Antibody [NBP2-15365] - Rat brain. Green: alpha Synuclein antibody diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488). Blue: Nuclear staining with Hoechst 33342.](http://images.novusbio.com/fullsize/Synuclein-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-15365-img0015.jpg)
![Immunohistochemistry Free-Floating: alpha-Synuclein Antibody (2A7) [NBP1-05194] - Analysis of rat cerebellum section costained with mouse mAb to alpha-synuclein NBP1-05194, dilution 1:1,000, in red, and rabbit pAb to GFAP, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The alpha-synuclein protein is concentrated in synaptic regions, while the GFAP antibody stains the filamentous cytoskeleton of Bergmann glia and astrocytic cells.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-2A7-Immunocytochemistry-Immunofluorescence-NBP1-05194-img0004.jpg "Immunohistochemistry Free-Floating: alpha-Synuclein Antibody (2A7) [NBP1-05194] - Analysis of rat cerebellum section costained with mouse mAb to alpha-synuclein NBP1-05194, dilution 1:1,000, in red, and rabbit pAb to GFAP, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The alpha-synuclein protein is concentrated in synaptic regions, while the GFAP antibody stains the filamentous cytoskeleton of Bergmann glia and astrocytic cells.")
![Simple Western: alpha-Synuclein Antibody (2A7) [NBP1-05194] - Synuclein-alpha Antibody (2A7) [NBP1-05194] - Simple Western lane view shows a specific band for alpha-Synuclein in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Synuclein-alpha-Antibody-2A7-Simple-Western-NBP1-05194-img0003.jpg)
![Western Blot: alpha-Synuclein Antibody (2A7) [NBP1-05194] - Western blot analysis of tissue lysates using mAb to alpha-synuclein NBP1-05194. dilution 1:1,000 in green. Lane [1] protein standard in red, [2] whole rat brain lysate, [3] rat spinal cord lysate. Strong band at about 15kDa corresponds to alpha-synuclein protein.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-2A7-Western-Blot-NBP1-05194-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Synuclein-alpha Antibody (2A7) [NBP1-05194] - Mixed neuron-glial cultures stained with NBP1-05194, our monoclonal antibody to alpha-synuclein (red) and chicken polylclonal antibody to MAP2 NB300-213 (green). The alpha-synuclein antibody stains vesicular structures the perikarya and processes of the neurons in this image. Note that some of the neuronal perikarya contain much more alpha-synuclein than others. The blue channel shows the localization of DNA.](http://images.novusbio.com/fullsize/Synuclein-alpha-Antibody-2A7-Immunocytochemistry-Immunofluorescence-NBP1-05194-img0002.jpg)
![Flow Cytometry: alpha-Synuclein Antibody (2A7) [NBP1-05194] - An intracellular stain was performed on SK-MEL-28 cells with alpha-Synuclein [2A7] Antibody NBP1-05194AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-2A7-Flow-Cytometry-NBP1-05194-img0006.jpg)
![Immunohistochemistry: alpha-Synuclein Antibody [NBP2-25146] - Immunofluorescent analysis of a section of rat cerebellum stained with chicken pAb to alpha-synuclein, NBP2-25146, dilution 1:3,000 in red, and costained with rabbit pAb to GFAP, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The alpha-synuclein protein is concentrated in presynaptic regions in the granular and molecular layers, while GFAP antibody stains the network of Bergmann and astroglial cells.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-Immunofluorescence-NBP2-25146-img0010.jpg "Immunohistochemistry: alpha-Synuclein Antibody [NBP2-25146] - Immunofluorescent analysis of a section of rat cerebellum stained with chicken pAb to alpha-synuclein, NBP2-25146, dilution 1:3,000 in red, and costained with rabbit pAb to GFAP, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The alpha-synuclein protein is concentrated in presynaptic regions in the granular and molecular layers, while GFAP antibody stains the network of Bergmann and astroglial cells.")
![Western Blot: alpha-Synuclein Antibody [NBP2-25146] - Western blot analysis of different tissue lysates using chicken pAb to alpha-synuclein, NBP2-25146, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at about 15kDa corresponds to the alpha-synuclein protein in brain extracts, which are rich in synapses, while a weaker band is seen in spinal cord extracts where synapses are a more minor component.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-Western-Blot-NBP2-25146-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Synuclein-alpha Antibody [NBP2-25146] - Mixed rat neuron-glial cultures stained with NBP2-25146, polyclonal antibody to alpha-Synuclein (red) and monoclonal antibody to MAP2 (NBP2-25156, green). The alpha-Synuclein antibody stains vesicular structures, the perikarya and processes of the neurons in this image. Note that some of the neuronal perikarya contain much more alpha-Synuclein than others. The blue channel shows the localization of DNA.](http://images.novusbio.com/fullsize/Synuclein-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-25146-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody [NBP2-25146] - Human iPS derived neurons, fixed in 4% formaldehyde solution. NBP2-25146 diluted 1:1500, followed by staining with Alexa anti chicken-594 (1:1000) secondary antibody. All antibodies were diluted in PBS/BSA 3% (w/v) ) Triton X-100 0,3 % (v/v). Images taken by an Arrayscan (Cellomics). Image from verified customer review.](http://images.novusbio.com/fullsize/Synuclein-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-25146-img0006.jpg)
![Western Blot: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Lane 1: Molecular Weight Ladder (MW). Lane 2: Active Alpha Synuclein Protein Aggregate.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Western-Blot-NBP2-54789-img0005.jpg "Western Blot: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Lane 1: Molecular Weight Ladder (MW). Lane 2: Active Alpha Synuclein Protein Aggregate.")
![Immunocytochemistry/Immunofluorescence: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Primary rat hippocampal neurons show lewy body inclusion formation when treated with active Alpha Synuclein Protein Aggregate at 4 ug/ml (D-F), but not when treated with control Alpha Synuclein Protein Aggregate at 4 ug/ml (A-C). Tissue: Primary hippocampal neurons. Species: Sprague-Dawley rat. Fixation: 4% formaldehyde from PFA. Primary Antibody: Mouse anti-pSer129 Antibody at 1:1000 24 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:700 for 1 hours at RT. Counterstain: Hoechst (blue) nuclear stain at 1:4000 for 1 hour at RT. Localization: Lewy body incluscions. Magnification: 20x.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Immunocytochemistry-Immunofluorescence-NBP2-54789-img0004.jpg)
![Immunohistochemistry: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Immunohistochemistry analysis of rat brain injected with Type 1 human alpha synuclein PFFs (NBP2-54789). Species: Female Sprague-Dawley Rat. Rat was injected with 16g Type 1 human alpha synuclein PFFs (NBP2-54789) in each of 2 injection sites: AP+1.6, ML+2.4, DV-4.2 from skull; and AP-1.4, ML+0.2, DV-2.8 from skull. 30-days post-injection. Fixation: Saline perfusion followed by 4% PFA fixation for 48 hrs. Primary antibody: rabbit monoclonal anti-pSer129 alpha synuclein. Secondary Antibody: Biotin-SP Donkey Anti-Rabbit IgG (H+L) at 1:500 for 2 hours in cold room with shaking. ABC signal amplification, DAB staining. Magnification: 20X. Alpha synuclein pathology is seen in the striatum close to an injection site.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Immunohistochemistry-NBP2-54789-img0007.jpg)
![In vitro assay: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate (NBP2-54789) is combined with 100 uM of active alpha Synuclein monomer (NBP2-54788), as compared to active alpha Synuclein aggregate (NBP2-54789) and active alpha Synuclein monomer (NBP2-54788) alone. Thioflavin T ex = 450 nm, em = 485 nm.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-In-vitro-assay-NBP2-54789-img0006.jpg)
![Electron Microscopy: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - TEM of Type 1 alpha-Synuclein Pre-formed Fibrils (PFFs) (NBP2-54789)](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Electron-Microscopy-NBP2-54789-img0008.jpg)
![Product Image: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Toxicity results comparing Active Human Recombinant alpha-Synuclein Pre-formed Fibrils (Type 2) and Active Human Recombinant alpha-Synuclein Pre-formed Fibrils (Type 1) (Catalog No. NBP2-54789). Data was graphed after live cell imaging results were obtained using the following procedure: After 8 days in vitro, primary rat mixed cortical neuron cells were washed with 1X PBS and treated with 500 g/ml of Type 1 and Type 2 alpha-Synuclein Proteins for 20 hours at 37C. Following treatements, cells were washed with 2X PBS and incubated with a staining solution (2.0 M Cell Event + 2.5 M Ethidium homodimer + 2.5 g/ml Hoechst 33342 in sterile HBSS) for 30 minutes at 37C. The addition of the Type 2 alpha-Synuclein Proteins resulted in a significant increase in cell death.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Product-Image-NBP2-54789-img0010.jpg)
![Electron Microscopy: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - TEM of Type 1 alpha-Synuclein Pre-formed Fibrils (PFFs) (NBP2-54789)](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Electron-Microscopy-NBP2-54789-img0009.jpg)
![Western Blot: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - Lane 1: Molecular Weight Ladder (MW). Lane 2: Alpha Synuclein Protein Aggregate](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-2-Protein-Western-Blot-NBP2-54787-img0005.jpg "Western Blot: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - Lane 1: Molecular Weight Ladder (MW). Lane 2: Alpha Synuclein Protein Aggregate")
![Immunocytochemistry/Immunofluorescence: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - Primary rat hippocampal neurons show lewy body inclusion formation when treated with active Alpha Synuclein Protein Aggregate at 4 ug/ml (D-F), but not when treated with control Alpha Synuclein Protein Aggregate at 4 ug/ml (A-C). Tissue: Primary hippocampal neurons. Species: Sprague-Dawley rat. Fixation: 4% formaldehyde made from PFA. Primary Antibody: Mouse anti-pSer129 Antibody at 1:1000 24 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:700 for 1 hours at RT. Counterstain: Hoechst (blue) nuclear stain at 1:4000 for 1 hour at RT. Localization: Lewy body incluscions. Magnification: 20x.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-2-Protein-Immunocytochemistry-Immunofluorescence-NBP2-54787-img0006.jpg)
![In vitro assay: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha Synuclein aggregates. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate (NBP2-54789) is combined with 100 uM of active alpha Synuclein monomer (NBP2-54788), as compared to when 100 uM of active alpha Synuclein monomer (NBP2-54788) is combined with 10 nM of control alpha Synuclein aggregate (NBP2-54787), or 100 uM of control alpha Synuclein monomer (NBP2-54786) is combined with 10 nM of control alpha Synuclein aggregate (NBP2-54787). Thioflavin T ex = 450 nm, em = 485 nm.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-2-Protein-In-vitro-assay-NBP2-54787-img0007.jpg)
![Product Image: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - Toxicity results comparing Active Human Recombinant alpha-Synuclein Pre-formed Fibrils (Type 2) (Catalog No. NBP2-54787) and Active Human Recombinant alpha-Synuclein Pre-formed Fibrils (Type 1). Data was graphed after live cell imaging results were obtained using the following procedure: After 8 days in vitro, primary rat mixed cortical neuron cells were washed with 1X PBS and treated with 500 g/ml of Type 1 and Type 2 alpha-Synuclein Proteins for 20 hours at 37C. Following treatements, cells were washed with 2X PBS and incubated with a staining solution (2.0 M Cell Event + 2.5 M Ethidium homodimer + 2.5 g/ml Hoechst 33342 in sterile HBSS) for 30 minutes at 37C. The addition of the Type 2 alpha-Synuclein Proteins resulted in a significant increase in cell death.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-2-Protein-Product-Image-NBP2-54787-img0009.jpg)
![Electron Microscopy: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 2) Protein [NBP2-54787] - TEM of Type 2 alpha-Synuclein Pre-formed Fibrils (PFFs) (NBP2-54787)](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-2-Protein-Electron-Microscopy-NBP2-54787-img0008.jpg)
![Western Blot: alpha-Synuclein Antibody (5C2) [NBP1-04321] - The recombinant human alpha-synuclein domains were resolved by SDS-PAGE, transferred to PVDF membrane and probed with human anti-alpha-Synuclein(61-95aa) antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Recombinant human alpha-synuclein(1-60aa)Lane 2.: Recombinant human alpha-synuclein(1-95aa)Lane 3.: Recombinant human alpha-synuclein(61-140aa)Lane 4.: Recombinant human alpha-synuclein(96-140aa)](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-5C2-Western-Blot-NBP1-04321-img0002.jpg "Western Blot: alpha-Synuclein Antibody (5C2) [NBP1-04321] - The recombinant human alpha-synuclein domains were resolved by SDS-PAGE, transferred to PVDF membrane and probed with human anti-alpha-Synuclein(61-95aa) antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Recombinant human alpha-synuclein(1-60aa)Lane 2.: Recombinant human alpha-synuclein(1-95aa)Lane 3.: Recombinant human alpha-synuclein(61-140aa)Lane 4.: Recombinant human alpha-synuclein(96-140aa)")
![Western Blot: alpha-Synuclein Antibody (5C2) [NBP1-04321] - The recombinant human synuclein family proteins were resolved by SDS-PAGE, transferred to PVDF membrane and probed with human anti-alpha-Synuclein antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Recombinant human alpha-synucleinLane 2.: Recombinant human beta-synucleinLane 3.: Recombinant human gamma-synuclein](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-5C2-Western-Blot-NBP1-04321-img0001.jpg)
![Immunohistochemistry-Paraffin: alpha-Synuclein Antibody (JB22-44) [NBP2-75409] - Analysis of paraffin-embedded mouse brain tissue using anti-Phospho-alpha Synuclein (S129) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-JB22-44-Immunohistochemistry-Paraffin-NBP2-75409-img0005.jpg "Immunohistochemistry-Paraffin: alpha-Synuclein Antibody (JB22-44) [NBP2-75409] - Analysis of paraffin-embedded mouse brain tissue using anti-Phospho-alpha Synuclein (S129) antibody. Counter stained with hematoxylin.")
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody (JB22-44) [NBP2-75409] - Staining Phospho-alpha Synuclein (S129) in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-JB22-44-Immunocytochemistry-Immunofluorescence-NBP2-75409-img0003.jpg)
![Immunohistochemistry-Paraffin: alpha-Synuclein Antibody (JB22-44) [NBP2-75409] - Analysis of paraffin-embedded rat brain tissue using anti-Phospho-alpha Synuclein (S129) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-JB22-44-Immunohistochemistry-Paraffin-NBP2-75409-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody (JB22-44) [NBP2-75409] - Staining Phospho-alpha Synuclein (S129) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-JB22-44-Immunocytochemistry-Immunofluorescence-NBP2-75409-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody (JB22-44) [NBP2-75409] - Staining Phospho-alpha Synuclein (S129) in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-JB22-44-Immunocytochemistry-Immunofluorescence-NBP2-75409-img0002.jpg)
![Immunohistochemistry-Paraffin: alpha-Synuclein Antibody (JB22-44) [NBP2-75409] - Analysis of paraffin-embedded human kidney tissue using anti-Phospho-alpha Synuclein (S129) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-JB22-44-Immunohistochemistry-Paraffin-NBP2-75409-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody (970106) [MAB13382] - Analysis using immersion fixed SK-Mel-28 human cytoplasm using SNCA antibody.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-970106-Immunocytochemistry-Immunofluorescence-MAB13382-img0001.jpg "Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody (970106) [MAB13382] - Analysis using immersion fixed SK-Mel-28 human cytoplasm using SNCA antibody.")
![Western Blot: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - Western Blot analysis of Human Alpha Synuclein showing detection of alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Monoclonal Antibody, Clone J18 (NBP3-18266). Lane 1: MW ladder. Lane 2: 0.5 ug human alpha synuclein monomer (SPR-321). Lane 3: 2 ug human alpha synuclein monomer (SPR-321). Lane 4: 0.5 ug human alpha synuclein PFFs (SPR-322). Lane 5: 2 ug human alpha synuclein PFFs (SPR-322). Lane 6: 15 ug human Parkinson's Disease brain lysate.. Block: 5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18266) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. NBP3-18266 does not detect unphosphorylated alpha synuclein.](http://images.novusbio.com/fullsize/alpha-Synuclein-p-Ser-129-Antibody-J18-Western-Blot-NBP3-18266-img0004.jpg "Western Blot: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - Western Blot analysis of Human Alpha Synuclein showing detection of alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Monoclonal Antibody, Clone J18 (NBP3-18266). Lane 1: MW ladder. Lane 2: 0.5 ug human alpha synuclein monomer (SPR-321). Lane 3: 2 ug human alpha synuclein monomer (SPR-321). Lane 4: 0.5 ug human alpha synuclein PFFs (SPR-322). Lane 5: 2 ug human alpha synuclein PFFs (SPR-322). Lane 6: 15 ug human Parkinson's Disease brain lysate.. Block: 5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18266) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. NBP3-18266 does not detect unphosphorylated alpha synuclein.")
![Immunohistochemistry: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - C57/BL6 mice were injected with sonicated recombinant mouse alpha synuclein monomers or fibrils at 8 weeks of age. Mice were unilaterally injected in the dorsal striatum (bregma AP + 0.2 mm, L +/1 2.0 mm, V - 3.0 mm) and sacrificed 30 days post-injection. (A) 1.25 uL mouse alpha synuclein monomers. (B) 2.5 uL mouse alpha synuclein monomers. (C) 2.5 ug alpha synuclein PFFs. (C) 5 ug alpha synuclein PFFs Inset: PBS (negative control). Primary antibody: Anti-alpha-Synuclein (NBP3-18266) at 1:10 000. Secondary antibody: anti-rabbit HRP. Mice injected with PFF displayed alpha synuclein staining in the striatum and cortex and contralateral to the injection site.](http://images.novusbio.com/fullsize/alpha-Synuclein-p-Ser-129-Antibody-J18-Immunohistochemistry-NBP3-18266-img0002.jpg)
![Western Blot: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - Western Blot analysis of Mouse Brain showing detection of alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Monoclonal Antibody, Clone J18 (NBP3-18266). Lane 1: MW ladder. Lane 2: Mouse brain. Load: 15 ug. Block: 5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18266) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Multiple bands due to phosphorylation.](http://images.novusbio.com/fullsize/alpha-Synuclein-p-Ser-129-Antibody-J18-Western-Blot-NBP3-18266-img0005.jpg)
![Immunohistochemistry: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - Immunohistochemistry analysis using Mouse Anti-alpha-Synuclein Monoclonal Antibody, Clone J18 (NBP3-18266). C57/BL6 mice were injected with 5 ug sonicated mouse recombinant alpha synuclein PFFs at 8 weeks of age. Mice were unilaterally injected in the dorsal striatum (bregma AP + 0.2 mm, L +/1 2.0 mm, V - 3.0 mm) and sacrificed 30 days post-injection. (A) contralateral cortex. (B) ipsilateral cortex. (C) contralateral striatum. (D) ipsilateral striatum. Primary Antibody: Mouse Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18266) at 1:10000. Secondary Antibody: anti-rabbit HRP. Courtesy of: Porsolt.](http://images.novusbio.com/fullsize/alpha-Synuclein-p-Ser-129-Antibody-J18-Immunohistochemistry-NBP3-18266-img0001.jpg)
![Dot Blot: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - Dot Blot analysis using Rabbit Anti-alpha-Synuclein Monoclonal Antibody, Clone J18 (NBP3-18266). Tissue: alpha synuclein peptide. Primary Antibody: Rabbit Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18266) at 1:500 for 2 hours at RT with shaking . Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking . Phospho peptide sequence: AYEMP-pS-EEGYQ. Non-phospho peptide sequence: AYEMPSEEGYQ. This sequence is the same for human, mouse, and rat.](http://images.novusbio.com/fullsize/alpha-Synuclein-p-Ser-129-Antibody-J18-Dot-Blot-NBP3-18266-img0003.jpg)