Product Search for novus: Novus Biologicals

Novus Biologicals products are now on bio-techne.com

Sort By:

Products (4)

page of 1

4 Results for "novus"

AF1369

alpha-Fetoprotein/AFP Antibody [Unconjugated]

(1 Review)

(6 Publications)

100 μg

AF1369

Western blot shows lysates of human liver tissue and H4‑II‑E‑C3 rat hepatoma cell line. PVDF membrane was probed with 0.2 µg/mL of Chicken Anti-Human/Rat alpha ‑Fetoprotein/ AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1369) followed by HRP-conjugated Anti-Chicken IgY Secondary Antibody. A specific band was detected for alpha ‑Fetoprotein/AFP at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

a-Fetoprotein/AFP was detected in immersion fixed BG01V human embryonic stem cells using Chicken Anti-Human/Rat a-Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Chicken IgY Secondary Antibody (red; Catalog # NL016) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

alpha ‑Fetoprotein/AFP was detected in immersion fixed paraffin-embedded sections of human liver cancer using 15 µg/mL Chicken anti-Human/ Rat alpha ‑Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1369) overnight at 4 °C. Tissue was stained (brown) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for alpha ‑Fetoprotein/AFP at approximately 70 kDa (as indicated) using 2 µg/mL of Chicken Anti-Human/Rat alpha ‑Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1369) followed by HRP-conjugated Anti-Chicken IgY Secondary Antibody. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

<b>Genetic Strategies Validation. </b>a-Fetoprotein/AFP was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line but is not detected in a-Fetoprotein/AFP knockout (KO) HepG2 Human Cell Line cell line using Chicken Anti-Human/Rat a-Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Chicken IgY Secondary Antibody (green; Catalog # NL018) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Chicken Polyclonal

Host: Chicken Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF1369

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1369-SP

compare

(select up to 3 products from this page to compare)

NBP1-76275

alpha-Fetoprotein/AFP Antibody - BSA Free

(4 Publications)

0.1 mg

NBP1-76275

Western Blot: alpha-Fetoprotein/AFP Antibody [NBP1-76275] - Analysis in fetal human liver tissue lysate with antibody at (A) 1 ug/mL and (B) 2 ug/mL.

Immunocytochemistry/Immunofluorescence: alpha-Fetoprotein/AFP Antibody [NBP1-76275] - Mouse liver tissue with AFP antibody at 20 ug/mL.

Immunohistochemistry: alpha-Fetoprotein/AFP Antibody [NBP1-76275] - Analysis of AFP in mouse liver tissue with AFP antibody at 2.5 ug/ml.

Immunocytochemistry/Immunofluorescence: alpha-Fetoprotein/AFP Antibody [NBP1-76275] - Rat liver tissue with AFP antibody at 20 ug/mL.

Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody [NBP1-76275] - Rat liver tissue with AFP antibody at 10 ug/mL.

Reactivity:

Hu, Mu, Rt, Po

Species Glossary

Reactivity: Hu, Mu, Rt, Po

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-76275AF350
Alexa Fluor 405	NBP1-76275AF405
Alexa Fluor 488	NBP1-76275AF488
Alexa Fluor 532	NBP1-76275AF532
Alexa Fluor 594	NBP1-76275AF594
Alexa Fluor 647	NBP1-76275AF647
Alexa Fluor 700	NBP1-76275AF700
Alexa Fluor 750	NBP1-76275AF750
Biotin	NBP1-76275B
DyLight 350	NBP1-76275UV
DyLight 405	NBP1-76275V
DyLight 488	NBP1-76275G
DyLight 550	NBP1-76275R
DyLight 594	NBP1-76275DL594
DyLight 650	NBP1-76275C
DyLight 680	NBP1-76275FR
DyLight 755	NBP1-76275IR
FITC	NBP1-76275F
HRP	NBP1-76275H
Janelia Fluor 549	NBP1-76275JF549
Janelia Fluor 646	NBP1-76275JF646
PerCP	NBP1-76275PCP
mFluor Violet 450 SE	NBP1-76275MFV450
mFluor Violet 500 SE	NBP1-76275MFV500
mFluor Violet 610 SE	NBP1-76275MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-76275-0.025mg

compare

(select up to 3 products from this page to compare)

NBP1-76275MFV610

alpha-Fetoprotein/AFP Antibody [mFluor Violet 610 SE]

0.1 ml

Reactivity:

Hu, Mu, Rt, Po

Species Glossary

Reactivity: Hu, Mu, Rt, Po

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

mFluor Violet 610 SE

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-76275AF350
Alexa Fluor 405	NBP1-76275AF405
Alexa Fluor 488	NBP1-76275AF488
Alexa Fluor 532	NBP1-76275AF532
Alexa Fluor 594	NBP1-76275AF594
Alexa Fluor 647	NBP1-76275AF647
Alexa Fluor 700	NBP1-76275AF700
Alexa Fluor 750	NBP1-76275AF750
Biotin	NBP1-76275B
DyLight 350	NBP1-76275UV
DyLight 405	NBP1-76275V
DyLight 488	NBP1-76275G
DyLight 550	NBP1-76275R
DyLight 594	NBP1-76275DL594
DyLight 650	NBP1-76275C
DyLight 680	NBP1-76275FR
DyLight 755	NBP1-76275IR
FITC	NBP1-76275F
HRP	NBP1-76275H
Janelia Fluor 549	NBP1-76275JF549
Janelia Fluor 646	NBP1-76275JF646
PerCP	NBP1-76275PCP
mFluor Violet 450 SE	NBP1-76275MFV450
mFluor Violet 500 SE	NBP1-76275MFV500

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-76275
BSA Free	NBP1-76275-0.025mg

compare

(select up to 3 products from this page to compare)

NBP1-76275PCP

alpha-Fetoprotein/AFP Antibody [PerCP]

0.1 ml

Reactivity:

Hu, Mu, Rt, Po

Species Glossary

Reactivity: Hu, Mu, Rt, Po

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

PerCP

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-76275AF350
Alexa Fluor 405	NBP1-76275AF405
Alexa Fluor 488	NBP1-76275AF488
Alexa Fluor 532	NBP1-76275AF532
Alexa Fluor 594	NBP1-76275AF594
Alexa Fluor 647	NBP1-76275AF647
Alexa Fluor 700	NBP1-76275AF700
Alexa Fluor 750	NBP1-76275AF750
Biotin	NBP1-76275B
DyLight 350	NBP1-76275UV
DyLight 405	NBP1-76275V
DyLight 488	NBP1-76275G
DyLight 550	NBP1-76275R
DyLight 594	NBP1-76275DL594
DyLight 650	NBP1-76275C
DyLight 680	NBP1-76275FR
DyLight 755	NBP1-76275IR
FITC	NBP1-76275F
HRP	NBP1-76275H
Janelia Fluor 549	NBP1-76275JF549
Janelia Fluor 646	NBP1-76275JF646
mFluor Violet 450 SE	NBP1-76275MFV450
mFluor Violet 500 SE	NBP1-76275MFV500
mFluor Violet 610 SE	NBP1-76275MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-76275
BSA Free	NBP1-76275-0.025mg

compare

(select up to 3 products from this page to compare)

page of 1

Popular Products	Company Information	Support	Stay Connected
Antibodies Secondary Antibodies Isotype Controls Proteins and Peptides ELISA Kits Sample Size New Products	About Us Careers Novus Guarantee Contact Us Our Team	Technical Support FAQs Help Finding Antibodies Protocols

Visit our other sites

©2024 Novus Biologicals, All Rights Reserved.