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![Immunohistochemistry: SOD2/Mn-SOD Antibody [NB100-1992] - The effects of a high calcium/phosphate diet, of 1,25(OH)2D3, and of antioxidant supplementation on oxidative stress, DNA damage, and protein expression of oncogenes and tumor suppressive genes in 1alpha(OH)ase -/- mice. Mice from each group were treated as described in Figure 1. Representative micrographs of skin sections stained immunohistochemically for SOD2 and gamma-H2AX. Scale bars represent 50 um. Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/abs/10.1111/acel.12951), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Immunohistochemistry-NB100-1992-img0013.jpg "<b>Biological Strategies Validation. </b>Immunohistochemistry: SOD2/Mn-SOD Antibody [NB100-1992] - The effects of a high calcium/phosphate diet, of 1,25(OH)2D3, and of antioxidant supplementation on oxidative stress, DNA damage, and protein expression of oncogenes and tumor suppressive genes in 1alpha(OH)ase -/- mice. Mice from each group were treated as described in Figure 1. Representative micrographs of skin sections stained immunohistochemically for SOD2 and gamma-H2AX. Scale bars represent 50 um. Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/abs/10.1111/acel.12951), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: SOD2/Mn-SOD Antibody [NB100-1992] - Analysis using Rabbit Anti-SOD (Mn) Polyclonal Antibody. Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-SOD (Mn) Polyclonal Antibody at 1:120 for 12 hours at 4 degrees C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 120 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Localization: Mitochondrion matrix. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-SOD (Mn) Antibody. (C) Composite.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1992-img0010.jpg)
![Western Blot: SOD2/Mn-SOD Antibody [NB100-1992] - Western blot analysis of Rat Tissue lysates showing detection of SOD2/Mn-SOD protein using Rabbit Anti-SOD2/Mn-SOD Polyclonal Antibody (NB100-1992). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-SOD2/Mn-SOD Polyclonal Antibody (NB100-1992) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Western-Blot-NB100-1992-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: SOD2/Mn-SOD Antibody [NB100-1992] - Analysis using Rabbit Anti-SOD (Mn) Polyclonal Antibody. Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-SOD (Mn) Polyclonal Antibody at 1:120 for 12 hours at 4 degrees C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 120 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Localization: Mitochondrion matrix. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-SOD (Mn) Antibody. (C) Composite.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1992-img0012.jpg)
![Immunohistochemistry: SOD2/Mn-SOD Antibody [NB100-1992] - Immunohistochemistry analysis using Rabbit Anti-SOD2/Mn-SOD Polyclonal Antibody (NB100-1992). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-SOD2/Mn-SOD Polyclonal Antibody (NB100-1992) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Mitochondrion matrix.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Immunohistochemistry-NB100-1992-img0017.jpg)
![ELISA SOD2/Mn-SOD [Biotin]](http://images.novusbio.com/fullsize2/dyc3419-2_human-mouse-rattotal-sod2-mn-sod-duoset-ic-elisa-2-plate-elisa-169202094430.jpg "ELISA SOD2/Mn-SOD [Biotin]")
![Western Blot: SOD2/Mn-SOD Antibody [NBP2-20535] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Western-Blot-NBP2-20535-img0016.jpg "<b>Genetic Strategies Validation. </b>Western Blot: SOD2/Mn-SOD Antibody [NBP2-20535] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: SOD2/Mn-SOD Antibody [NBP2-20535] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20535-img0006.jpg)
![Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody [NBP2-20535] - Human breast carcinoma. SOD2 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Immunohistochemistry-Paraffin-NBP2-20535-img0014.jpg)
![Western Blot: SOD2/Mn-SOD Antibody [NBP2-20535] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody diluted by 1:1000.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Western-Blot-NBP2-20535-img0011.jpg)
![Western Blot: SOD2/Mn-SOD Antibody [NBP2-20535] - Mouse tissue extracts (50 ug) was separated by 15 % SDS-PAGE, and the membrane was blotted with SOD2 antibody diluted by 1:1000.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Western-Blot-NBP2-20535-img0012.jpg)
![Western Blot: SOD2/Mn-SOD Antibody [NBP2-20535] - Rat tissue extracts (50 ug) was separated by 15 % SDS-PAGE, and the membrane was blotted with SOD2 antibody diluted by 1:2500.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Western-Blot-NBP2-20535-img0013.jpg)
![Western Blot: SOD2/Mn-SOD Antibody [NB100-1969] - Western blot analysis of Mouse Brain, Rat Brain, and Rat Brain Membrane showing detection of 24.7kDa SOD (Mn) protein using Rabbit Anti-SOD (Mn) Polyclonal Antibody (NB100-1969). Lane 1: MW Ladder. Lane 2: Mouse Brain (15 ug). Lane 3: Rat Brain (15 ug). Lane 4: Rat Brain Membrane (15 ug). Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-SOD (Mn) Polyclonal Antibody (NB100-1969) at 1:1000 for O/N at 4C. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:5000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: 24.7kDa.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Western-Blot-NB100-1969-img0002.jpg "Western Blot: SOD2/Mn-SOD Antibody [NB100-1969] - Western blot analysis of Mouse Brain, Rat Brain, and Rat Brain Membrane showing detection of 24.7kDa SOD (Mn) protein using Rabbit Anti-SOD (Mn) Polyclonal Antibody (NB100-1969). Lane 1: MW Ladder. Lane 2: Mouse Brain (15 ug). Lane 3: Rat Brain (15 ug). Lane 4: Rat Brain Membrane (15 ug). Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-SOD (Mn) Polyclonal Antibody (NB100-1969) at 1:1000 for O/N at 4C. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:5000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: 24.7kDa.")
![Western Blot: SOD2/Mn-SOD Antibody [NBP1-86169] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Western-Blot-NBP1-86169-img0006.jpg "Western Blot: SOD2/Mn-SOD Antibody [NBP1-86169] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody [NBP1-86169] - Staining of human skeletal muscle shows moderate granular cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Immunohistochemistry-Paraffin-NBP1-86169-img0010.jpg)
![Western Blot: SOD2/Mn-SOD Antibody [NBP1-86169] - Analysis in human cell line U-87 MG.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Western-Blot-NBP1-86169-img0005.jpg)
![Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody [NBP1-86169] - Staining of human colon shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Immunohistochemistry-Paraffin-NBP1-86169-img0007.jpg)
![Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody [NBP1-86169] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Immunohistochemistry-Paraffin-NBP1-86169-img0009.jpg)
![Western Blot: SOD2/Mn-SOD Antibody (582) [NBP2-43795] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody [582].](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-582-Western-Blot-NBP2-43795-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: SOD2/Mn-SOD Antibody (582) [NBP2-43795] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody [582].")
![<b>Genetic Strategies Validation. </b>Western Blot: SOD2/Mn-SOD Antibody (582) [NBP2-43795] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract | D. 30 ug HepG2 whole cell lysate/extract 12 % SDS-PAGE SOD2 antibody [582] dilution: 1:5000](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-582-Western-Blot-NBP2-43795-img0001.jpg)
![Western Blot: SOD2/Mn-SOD Antibody (582) [NBP2-43795] - Analysis of A. 50 ug mouse brain lysate/extract 12 % SDS-PAGE SOD2 antibody [582] dilution: 1:5000](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-582-Western-Blot-NBP2-43795-img0002.jpg)
![Western Blot: SOD2/Mn-SOD Antibody (582) [NBP2-43795] - Analysis of A. 50 ug rat brain lysate/extract 12 % SDS-PAGE SOD2 antibody [582] dilution: 1:5000.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-582-Western-Blot-NBP2-43795-img0003.jpg)
![Western Blot: SOD2/Mn-SOD [ac Lys68] Antibody (HL1460) - Azide and BSA Free [NBP3-25516] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SOD2 (Acetyl Lys68) antibody [HL1460] (NBP3-25516) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25516_rabbit-sod2-mn-sod-ac-lys-68-mab-hl1460-azide-bsa-free-2120249351334.jpg "Western Blot: SOD2/Mn-SOD [ac Lys68] Antibody (HL1460) - Azide and BSA Free [NBP3-25516] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SOD2 (Acetyl Lys68) antibody [HL1460] (NBP3-25516) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: SOD2/Mn-SOD [ac Lys68] Antibody (HL1460) - Azide and BSA Free [NBP3-25516] - Whole cell extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with SOD2 (Acetyl Lys68) antibody [HL1460] (NBP3-25516) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25516_rabbit-sod2-mn-sod-ac-lys-68-mab-hl1460-azide-bsa-free-2120249362787.jpg)
![SDS-Page: SOD2/Mn-SOD Recombinant Protein [NBP2-52079] - 15% SDS Page (3 ug)](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Recombinant-Protein-SDS-Page-NBP2-52079-img0001.jpg "SDS-Page: SOD2/Mn-SOD Recombinant Protein [NBP2-52079] - 15% SDS Page (3 ug)")