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![Western Blot: PUF60 Antibody [NBP2-49303] - Analysis using Anti-PUF60 antibody NBP2-49303 (A) shows similar pattern to independent antibody NBP2-49032 (B).](http://images.novusbio.com/fullsize/PUF60-Antibody-Western-Blot-NBP2-49303-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PUF60 Antibody [NBP2-49303] - Analysis using Anti-PUF60 antibody NBP2-49303 (A) shows similar pattern to independent antibody NBP2-49032 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: PUF60 Antibody [NBP2-49303] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-49303-img0008.jpg)
![Immunohistochemistry-Paraffin: PUF60 Antibody [NBP2-49303] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-Paraffin-NBP2-49303-img0013.jpg)
![Immunohistochemistry-Paraffin: PUF60 Antibody [NBP2-49303] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-Paraffin-NBP2-49303-img0005.jpg)
![Immunohistochemistry-Paraffin: PUF60 Antibody [NBP2-49303] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-Paraffin-NBP2-49303-img0006.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PUF60 Antibody [NBP2-49303] - Staining in human testis and pancreas tissues using anti-PUF60 antibody. Corresponding PUF60 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-Paraffin-NBP2-49303-img0007.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PUF60 Antibody [NBP2-49303] - Staining of human cerebral cortex, colon, kidney and testis using Anti-PUF60 antibody NBP2-49303 (A) shows similar protein distribution across tissues to independent antibody NBP2-49032 (B).](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-Paraffin-NBP2-49303-img0009.jpg)
![Immunohistochemistry-Paraffin: PUF60 Antibody [NBP2-49303] - Staining of human colon.](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-Paraffin-NBP2-49303-img0011.jpg)
![Immunohistochemistry-Paraffin: PUF60 Antibody [NBP2-49303] - Staining of human kidney.](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-Paraffin-NBP2-49303-img0012.jpg)
![Western Blot: PUF60 Antibody [NBP2-49032] - Analysis using Anti-PUF60 antibody NBP2-49032 (A) shows similar pattern to independent antibody NBP2-49303 (B).](http://images.novusbio.com/fullsize/PUF60-Antibody-Western-Blot-NBP2-49032-img0007.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PUF60 Antibody [NBP2-49032] - Analysis using Anti-PUF60 antibody NBP2-49032 (A) shows similar pattern to independent antibody NBP2-49303 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PUF60 Antibody [NBP2-49032] - Staining of human kidney.](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-Paraffin-NBP2-49032-img0009.jpg)
![Immunohistochemistry: PUF60 Antibody [NBP2-49032] - Staining of human colon shows strong nuclear and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-NBP2-49032-img0001.jpg)
![Immunohistochemistry-Paraffin: PUF60 Antibody [NBP2-49032] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-Paraffin-NBP2-49032-img0003.jpg)
![Immunohistochemistry-Paraffin: PUF60 Antibody [NBP2-49032] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-Paraffin-NBP2-49032-img0004.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PUF60 Antibody [NBP2-49032] - Staining in human testis and pancreas tissues using anti-PUF60 antibody. Corresponding PUF60 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-Paraffin-NBP2-49032-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PUF60 Antibody [NBP2-49032] - Staining of human cerebral cortex, colon, kidney and testis using Anti-PUF60 antibody NBP2-49032 (A) shows similar protein distribution across tissues to independent antibody NBP2-49303 (B).](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-Paraffin-NBP2-49032-img0006.jpg)
![Immunohistochemistry-Paraffin: PUF60 Antibody [NBP2-49032] - Staining of human colon.](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-Paraffin-NBP2-49032-img0008.jpg)
![Immunohistochemistry-Paraffin: PUF60 Antibody [NBP2-49032] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-Paraffin-NBP2-49032-img0010.jpg)
![Western Blot: PUF60 Antibody (3510) [NBP2-43835] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 7.5 % SDS-PAGE PUF60 antibody [3510] dilution: 1:5000](http://images.novusbio.com/fullsize/PUF60-Antibody-3510-Western-Blot-NBP2-43835-img0004.jpg "Western Blot: PUF60 Antibody (3510) [NBP2-43835] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 7.5 % SDS-PAGE PUF60 antibody [3510] dilution: 1:5000")
![Immunocytochemistry/Immunofluorescence: PUF60 Antibody (3510) [NBP2-43835] - Analysis of Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PUF60 protein stained by PUF60 antibody [3510] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/PUF60-Antibody-3510-Immunocytochemistry-Immunofluorescence-NBP2-43835-img0005.jpg)
![Immunohistochemistry-Paraffin: PUF60 Antibody (3510) [NBP2-43835] - Analysis of gastric carcinoma. PUF60 antibody [3510] dilution: 1:100.](http://images.novusbio.com/fullsize/PUF60-Antibody-3510-Immunohistochemistry-Paraffin-NBP2-43835-img0007.jpg)
![Western Blot: PUF60 Antibody (3510) [NBP2-43835] - Analysis of A. 50 ug mouse brain lysate/extract 7.5 % SDS-PAGE PUF60 antibody [3510] dilution: 1:1000](http://images.novusbio.com/fullsize/PUF60-Antibody-3510-Western-Blot-NBP2-43835-img0002.jpg)
![Western Blot: PUF60 Antibody (3510) [NBP2-43835] - Analysis of A. 50 ug rat brain lysate/extract 7.5 % SDS-PAGE PUF60 antibody [3510] dilution: 1:1000](http://images.novusbio.com/fullsize/PUF60-Antibody-3510-Western-Blot-NBP2-43835-img0003.jpg)
![Immunohistochemistry-Paraffin: PUF60 Antibody (3510) [NBP2-43835] - Analysis of SAS xenograft. PUF60 antibody [3510] dilution: 1:100.](http://images.novusbio.com/fullsize/PUF60-Antibody-3510-Immunohistochemistry-Paraffin-NBP2-43835-img0006.jpg)
![Western Blot: PUF60 Antibody (677) [NBP2-43836] - Analysis of A. 50 ug rat brain lysate/extract 7.5 % SDS-PAGE PUF60 antibody [677] dilution: 1:1000](http://images.novusbio.com/fullsize/PUF60-Antibody-677-Western-Blot-NBP2-43836-img0004.jpg "Western Blot: PUF60 Antibody (677) [NBP2-43836] - Analysis of A. 50 ug rat brain lysate/extract 7.5 % SDS-PAGE PUF60 antibody [677] dilution: 1:1000")
![Immunocytochemistry/Immunofluorescence: PUF60 Antibody (677) [NBP2-43836] - Analysis of A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PUF60 protein stained by PUF60 antibody [677] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/PUF60-Antibody-677-Immunocytochemistry-Immunofluorescence-NBP2-43836-img0001.jpg)
![Immunohistochemistry-Paraffin: PUF60 Antibody (677) [NBP2-43836] - AGS xenograft. PUF60 antibody [677] dilution: 1:100. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/PUF60-Antibody-677-Immunohistochemistry-Paraffin-NBP2-43836-img0005.jpg)
![Western Blot: PUF60 Antibody (677) [NBP2-43836] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 7.5 % SDS-PAGE PUF60 antibody [677] dilution: 1:5000](http://images.novusbio.com/fullsize/PUF60-Antibody-677-Western-Blot-NBP2-43836-img0002.jpg)
![Western Blot: PUF60 Antibody (677) [NBP2-43836] - Analysis of A. 50 ug mouse brain lysate/extract 7.5 % SDS-PAGE PUF60 antibody [677] dilution: 1:1000](http://images.novusbio.com/fullsize/PUF60-Antibody-677-Western-Blot-NBP2-43836-img0003.jpg)
![Western Blot: PUF60 Antibody (917) [NBP2-43764] - Analysis of A. 50 ug rat brain lysate/extract 7.5 % SDS-PAGE PUF60 antibody [917] dilution: 1:1000.](http://images.novusbio.com/fullsize/PUF60-Antibody-917-Western-Blot-NBP2-43764-img0003.jpg "Western Blot: PUF60 Antibody (917) [NBP2-43764] - Analysis of A. 50 ug rat brain lysate/extract 7.5 % SDS-PAGE PUF60 antibody [917] dilution: 1:1000.")
![Immunohistochemistry-Paraffin: PUF60 Antibody (917) [NBP2-43764] - PUF60 antibody (917) detects PUF60 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. PUF60 stained by PUF60 antibody (917) diluted at 1:200.](http://images.novusbio.com/fullsize/PUF60-Antibody-917-Immunohistochemistry-Paraffin-NBP2-43764-img0006.jpg)
![Western Blot: PUF60 Antibody (917) [NBP2-43764] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 7.5 % SDS-PAGE PUF60 antibody [917] dilution: 1:1000](http://images.novusbio.com/fullsize/PUF60-Antibody-917-Western-Blot-NBP2-43764-img0001.jpg)
![Western Blot: PUF60 Antibody (917) [NBP2-43764] - Analysis of A. 50 ug mouse brain lysate/extract 7.5 % SDS-PAGE PUF60 antibody [917] dilution: 1:1000](http://images.novusbio.com/fullsize/PUF60-Antibody-917-Western-Blot-NBP2-43764-img0002.jpg)
![Immunohistochemistry-Paraffin: PUF60 Antibody (917) [NBP2-43764] - PUF60 antibody (917) detects PUF60 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. PUF60 stained by PUF60 antibody (917) diluted at 1:200.](http://images.novusbio.com/fullsize/PUF60-Antibody-917-Immunohistochemistry-Paraffin-NBP2-43764-img0004.jpg)
![Immunohistochemistry-Paraffin: PUF60 Antibody (917) [NBP2-43764] - PUF60 antibody (917) detects PUF60 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. PUF60 stained by PUF60 antibody PUF60 antibody (917) diluted at 1:200.](http://images.novusbio.com/fullsize/PUF60-Antibody-917-Immunohistochemistry-Paraffin-NBP2-43764-img0005.jpg)
![Western Blot: PUF60 Antibody [NB100-1125] - Staining of A431 lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PUF60-Antibody-Western-Blot-NB100-1125-img0002.jpg "Western Blot: PUF60 Antibody [NB100-1125] - Staining of A431 lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: PUF60 Antibody [NB100-1125] - Staining of paraffin embedded Human Skin. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-Paraffin-NB100-1125-img0005.jpg)
![PUF60 Antibody [NB100-1125] - Staining of paraffin embedded Human Prostate. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/PUF60-Antibody-NB100-1125-img0004.jpg)
![Western Blot: PUF60 Antibody [NBP2-20007] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/PUF60-Antibody-Western-Blot-NBP2-20007-img0001.jpg "Western Blot: PUF60 Antibody [NBP2-20007] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: PUF60 Antibody [NBP2-20007] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PUF60 protein stained by PUF60 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20007-img0005.jpg)
![Immunohistochemistry-Paraffin: PUF60 Antibody [NBP2-20007] - Paraffin-embedded U251 xenograft. PUF60 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-Paraffin-NBP2-20007-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: PUF60 Antibody [NBP2-20007] - A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PUF60 protein stained by PUF60 antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20007-img0003.jpg)
![Western Blot: PUF60 Antibody [NBP1-57501] - Antibody Titration: 0.2-1 ug/ml Positive control: HelaPUF60 is supported by BioGPS gene expression data to be expressed in HeLa.](http://images.novusbio.com/fullsize/PUF60-Antibody-Western-Blot-NBP1-57501-img0003.jpg "Western Blot: PUF60 Antibody [NBP1-57501] - Antibody Titration: 0.2-1 ug/ml Positive control: HelaPUF60 is supported by BioGPS gene expression data to be expressed in HeLa.")
![Immunohistochemistry: PUF60 Antibody [NBP1-57501]](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-NBP1-57501-img0004.jpg)
![Western Blot: PUF60 Antibody [NBP1-57501] - Transfected 293T Cell Lysate 5ug/ml Gel Concentration 12%](http://images.novusbio.com/fullsize/PUF60-Antibody-Western-Blot-NBP1-57501-img0001.jpg)
![Immunohistochemistry-Paraffin: PUF60 Antibody [NBP1-57501] - Human Intestine Tissue, antibody concentration 4-8ug/ml. Cells with positive label: Epithelial cells of intestinal villus (indicated with arrows) 400X magnification.](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-Paraffin-NBP1-57501-img0002.jpg)
![Western Blot: PUF60 Antibody [NBP1-49906] - Detection of Human PUF60 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-PUF60 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. PUF60 was also immunoprecipitated by rabbit anti-PUF60 antibodies, which recognize upstream epitopes. For blotting immunoprecipitated PUF60 were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/PUF60-Antibody-Western-Blot-NBP1-49906-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: PUF60 Antibody [NBP1-49906] - Detection of Human PUF60 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-PUF60 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. PUF60 was also immunoprecipitated by rabbit anti-PUF60 antibodies, which recognize upstream epitopes. For blotting immunoprecipitated PUF60 were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: PUF60 Antibody [NBP1-49906] - FFPE section of human prostate carcinoma (left) and mouse teratoma (right).Antibody: Affinity purified rabbit anti-PUF60 used at a dilution of 1:200 (1ug/ml)Detection: DAB](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-NBP1-49906-img0003.jpg)
![Western Blot: PUF60 Antibody (1C1) [H00022827-M20] - Analysis of PUF60 expression in transfected 293T cell line by PUF60 monoclonal antibody (M20), clone 1C1. Lane 1: PUF60 transfected lysate (Predicted MW: 59.9 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PUF60-Antibody-1C1-Western-Blot-H00022827-M20-img0004.jpg "Western Blot: PUF60 Antibody (1C1) [H00022827-M20] - Analysis of PUF60 expression in transfected 293T cell line by PUF60 monoclonal antibody (M20), clone 1C1. Lane 1: PUF60 transfected lysate (Predicted MW: 59.9 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: PUF60 Antibody (1C1) [H00022827-M20] - Analysis of monoclonal antibody to PUF60 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/PUF60-Antibody-1C1-Immunocytochemistry-Immunofluorescence-H00022827-M20-img0002.jpg)
![Sandwich ELISA: PUF60 Antibody (1C1) [H00022827-M20] - Detection limit for recombinant GST tagged PUF60 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PUF60-Antibody-1C1-Sandwich-ELISA-H00022827-M20-img0003.jpg)
![Western Blot: PUF60 Antibody [NBP1-57427] - Transfected 293T Cell Lysate 1.25ug/ml Gel Concentration 12%](http://images.novusbio.com/fullsize/PUF60-Antibody-Western-Blot-NBP1-57427-img0001.jpg "Western Blot: PUF60 Antibody [NBP1-57427] - Transfected 293T Cell Lysate 1.25ug/ml Gel Concentration 12%")
![Immunohistochemistry-Paraffin: PUF60 Antibody [NBP1-57427] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-Paraffin-NBP1-57427-img0002.jpg)
