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35 Results for "novus"
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AF847

PTEN Antibody [Unconjugated]

(14 Publications)
100 μg
AF847
Western blot shows lysates of mouse and rat brain tissue, A431 human epithelial carcinoma cell line, and MRC-5 human embryonic lung fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL Rabbit Anti-Human/Mouse/Rat PTEN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF847) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). For additional reference, recombinant human PTEN (5 ng) was included. A specific band for PTEN was detected at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, Simple Western, IHC, CyTOF-ready, Flow, KO
Applications: WB, Simple Western, IHC, CyTOF-ready, Flow, KO
Rabbit Polyclonal
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
         
Validated by:          
Conjugate Catalog # Availability Size Price
Biotin BAF847
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF847-SP
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MAB847

PTEN Antibody (217702) [Unconjugated]

(6 Publications)
100 μg
MAB847
Western blot shows lysates of mouse brain tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse PTEN Monoclonal Antibody (Catalog # MAB847) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for PTEN at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.**For Western blot, we recommend the use of Rabbit Anti-Human/Mouse/Rat PTEN Affinitiy-purified Polyclonal Ab (AF847).
Hu, Mu
Species Glossary
Reactivity: Hu, Mu
WB, IHC, CyTOF-ready, Flow, KO
Applications: WB, IHC, CyTOF-ready, Flow, KO
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 IC847U-100UG
Alexa Fluor 405 IC847V-100UG
Alexa Fluor 488 IC847G-100UG
Alexa Fluor 532 FAB847X
Alexa Fluor 594 IC847T-100UG
Alexa Fluor 647 IC847R-100UG
Alexa Fluor 700 IC847N-100UG
Alexa Fluor 750 IC847S-100UG
Allophycocyanin FAB847A
Biotin FAB847B
DyLight 350 FAB847D
DyLight 405 FAB847E
DyLight 488 FAB847K
DyLight 550 FAB847L
DyLight 594 FAB847M
DyLight 650 FAB847W
DyLight 680 FAB847Y
DyLight 755 FAB847Z
FITC FAB847F
HRP FAB847H
Janelia Fluor 549 FAB847I
Janelia Fluor 646 FAB847J
PE FAB847P
PerCP FAB847C
mFluor Violet 450 SE FAB847MFV450
mFluor Violet 500 SE FAB847MFV500
mFluor Violet 610 SE FAB847MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB847-SP
Carrier Free IC847T-100UG
Carrier Free IC847V-100UG
Carrier Free IC847G-100UG
Carrier Free IC847S-100UG
Carrier Free IC847U-100UG
Carrier Free IC847R-100UG
Carrier Free IC847N-100UG
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MAB847R

PTEN Antibody (217702R) [Unconjugated]

100 μg
MAB847R
Western blot shows lysates of 3T3‑L1 mouse embryonic fibroblast adipose-like cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse PTEN Monoclonal Antibody (Catalog # MAB847R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for PTEN at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Hu, Mu
Species Glossary
Reactivity: Hu, Mu
WB, Flow, KO
Applications: WB, Flow, KO
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 IC847RU-100UG
Alexa Fluor 350 FAB847RU
Alexa Fluor 405 IC847RV-100UG
Alexa Fluor 405 FAB847RV
Alexa Fluor 488 FAB847RG
Alexa Fluor 488 IC847RG-100UG
Alexa Fluor 532 FAB847RX
Alexa Fluor 594 FAB847RT
Alexa Fluor 594 IC847RT-100UG
Alexa Fluor 647 IC847RR-100UG
Alexa Fluor 647 FAB847RR
Alexa Fluor 700 IC847RN-100UG
Alexa Fluor 700 FAB847RN
Alexa Fluor 750 FAB847RS
Alexa Fluor 750 IC847RS-100UG
Allophycocyanin FAB847RA
Biotin FAB847RB
DyLight 350 FAB847RD
DyLight 405 FAB847RE
DyLight 488 FAB847RK
DyLight 550 FAB847RL
DyLight 594 FAB847RM
DyLight 650 FAB847RW
DyLight 680 FAB847RY
DyLight 755 FAB847RZ
FITC FAB847RF
HRP FAB847RH
Janelia Fluor 549 FAB847RI
Janelia Fluor 646 FAB847RJ
PE FAB847RP
PerCP FAB847RC
mFluor Violet 450 SE FAB847RMFV450
mFluor Violet 500 SE FAB847RMFV500
mFluor Violet 610 SE FAB847RMFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB847R-SP
Carrier Free IC847RU-100UG
Carrier Free IC847RR-100UG
Carrier Free IC847RS-100UG
Carrier Free IC847RG-100UG
Carrier Free IC847RT-100UG
Carrier Free IC847RN-100UG
Carrier Free IC847RV-100UG
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NBP3-15666

PTEN Antibody (7O6F4)

100 ul
NBP3-15666
Western Blot: PTEN Antibody (7O6F4) [NBP3-15666] - Analysis of extracts of C6 cells, using PTEN antibody (NBP3-15666) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, ICC/IF, IHC, IP, KO
Applications: WB, ICC/IF, IHC, IP, KO
Rabbit Monoclonal
Host: Rabbit Monoclonal
Unconjugated  
         
Validated by:          
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AF6655

PTEN Antibody [Unconjugated]

(2 Publications)
100 μg
AF6655
PTEN was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti- Human PTEN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6655) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Hu
Species Glossary
Reactivity: Hu
IHC, ICC/IF
Applications: IHC, ICC/IF
Goat Polyclonal
Host: Goat Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF6655-SP
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AF2838

PTEN [p Ser380] Antibody

(2 Publications)
100 μg
AF2838
Western blot shows Human/Mouse/Rat PTEN Monoclonal Antibody (Catalog # MAB847) immunopreciptate of MRC‑5 human embryonic lung fibroblast cell line, mouse and rat brain tissue untreated (-) or treated (+) with 300 U/mL CIP for 1 hour. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-PTEN (S380) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2838), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-PTEN (S380) at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB
Applications: WB
Rabbit Polyclonal
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF2838-SP
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NBP3-21201

PTEN Antibody (CL11723)

100 ul
NBP3-21201
Analysis in human cell line CAPAN-2.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, IHC
Applications: WB, IHC
Mouse Monoclonal
Host: Mouse Monoclonal
Unconjugated  
         
Validated by:          
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NBP3-21200

PTEN Antibody (CL11722)

100 ul
NBP3-21200
Staining of human skeletal muscle shows no positivity in myocytes as expected.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, IHC
Applications: WB, IHC
Mouse Monoclonal
Host: Mouse Monoclonal
Unconjugated  
         
Validated by:          
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847-PN

Recombinant Human PTEN Protein, CF

(3 Publications)
10 μg
Hu
Species Glossary
Reactivity: Hu
Inhibition Activity
Applications: Inhibition Activity
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NB100-2229

PTEN Antibody

(1 Publication)
0.1 ml
NB100-2229
Western Blot: PTEN Antibody [NB100-2229] - Detection of Human and Mouse PTEN on HeLa whole cell lysate using NB100-2229. PTEN was also IPed by rabbit anti-PTEN antibodies NB100-2227, NB100-2228 and another PTEN antibody.
Hu, Mu, Ca
Species Glossary
Reactivity: Hu, Mu, Ca
WB, IHC, IP
Applications: WB, IHC, IP
Rabbit Polyclonal
Host: Rabbit Polyclonal
Unconjugated  
         
Validated by:          
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NBP3-12919

PTEN [p Thr366, p Ser370] Antibody

100 ul
NBP3-12919
Western Blot: PTEN [p Thr366, p Ser370] Antibody [NBP3-12919] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PTEN (phospho Thr366/Ser370) antibody (NBP3-12919) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Hu, Mu, Rt, Po, Bv, Ca, RM, Xp
Species Glossary
Reactivity: Hu, Mu, Rt, Po, Bv, Ca, RM, Xp
WB, ICC/IF
Applications: WB, ICC/IF
Rabbit Polyclonal
Host: Rabbit Polyclonal
Unconjugated  
         
Validated by:          
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MAB66551

PTEN Antibody (1042838) [Unconjugated]

100 μg
MAB66551
PTEN was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line but is not detected in PTEN knockout (KO) HeLa Human Cell Line cell line using Mouse Anti-Human PTEN Monoclonal Antibody (Catalog # MAB66551) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Hu
Species Glossary
Reactivity: Hu
ICC/IF, KO
Applications: ICC/IF, KO
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB66551-SP
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