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![Western Blot: ISG15/UCRP Antibody (HL2017) - Azide and BSA Free [NBP3-25537] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with ISG15 antibody [HL2017] (NBP3-25537) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25537_rabbit-isg15-ucrp-mab-hl2017-azide-and-bsa-free-2120249432865.jpg "Western Blot: ISG15/UCRP Antibody (HL2017) - Azide and BSA Free [NBP3-25537] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with ISG15 antibody [HL2017] (NBP3-25537) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Western Blot: ISG15/UCRP Antibody (HL2017) - Azide and BSA Free [NBP3-25537] - Untreated (-) and treated (+) A549 whole cell extract (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ISG15 antibody [HL2017] (NBP3-25537) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25537_rabbit-isg15-ucrp-mab-hl2017-azide-and-bsa-free-2120249371565.jpg)
![Immunocytochemistry/Immunofluorescence: ISG15/UCRP Antibody (HL2017) - Azide and BSA Free [NBP3-25537] - ISG15 antibody [HL2017] detects ISG15 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: ISG15 stained by ISG15 antibody [HL2017] (NBP3-25537) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp3-25537_rabbit-isg15-ucrp-mab-hl2017-azide-and-bsa-free-2120249351399.jpg)
![Western Blot: ISG15/UCRP Antibody (HL2017) - Azide and BSA Free [NBP3-25537] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ISG15 antibody [HL2017] (NBP3-25537) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25537_rabbit-isg15-ucrp-mab-hl2017-azide-and-bsa-free-2120249342733.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: ISG15/UCRP Antibody (HL2017) - Azide and BSA Free [NBP3-25537] - Wild-type (WT) and ISG15 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ISG15 antibody [HL2017] (NBP3-25537) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25537_rabbit-isg15-ucrp-mab-hl2017-azide-and-bsa-free-2120249383112.jpg)
![Immunohistochemistry-Paraffin: ISG15/UCRP Antibody (HL2017) - Azide and BSA Free [NBP3-25537] - ISG15 antibody [HL2017] detects ISG15 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded cat brain. ISG15 stained by ISG15 antibody [HL2017] (NBP3-25537) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25537_rabbit-isg15-ucrp-mab-hl2017-azide-and-bsa-free-212024940821.jpg)
![Knockout Validated: ISG15/UCRP Antibody [NBP2-16995] - Wild-type (WT) and ISG15 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ISG15 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/ISG15-UCRP-Antibody-Western-Blot-NBP2-16995-img0004.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: ISG15/UCRP Antibody [NBP2-16995] - Wild-type (WT) and ISG15 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ISG15 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Genetic Strategies Validation. </b>Western Blot: ISG15/UCRP Antibody [NBP2-16995] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with ISG15 antibody diluted at 1:2000.](http://images.novusbio.com/fullsize/ISG15-UCRP-Antibody-Western-Blot-NBP2-16995-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ISG15/UCRP Antibody [NBP2-16995] - HepG2 cells were fixed in 4% paraformaldehyde for 10 min. Green: ISG15 protein stained by ISG15 antibody diluted at 1:100. Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/ISG15-UCRP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16995-img0006.jpg)
![Immunohistochemistry-Paraffin: ISG15/UCRP Antibody [NBP2-16995] - Human gastric cancer, using ISG15 antibody antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ISG15-UCRP-Antibody-Immunohistochemistry-Paraffin-NBP2-16995-img0002.jpg)
![Western Blot: ISG15/UCRP Antibody (ARC53794) [NBP3-16129] - Western blot analysis of extracts of various cell lines, using ISG15/UCRP Rabbit mAb (NBP3-16129) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/ISG15-UCRP-Antibody-1K3G7-Western-Blot-NBP3-16129-img0004.jpg "Western Blot: ISG15/UCRP Antibody (ARC53794) [NBP3-16129] - Western blot analysis of extracts of various cell lines, using ISG15/UCRP Rabbit mAb (NBP3-16129) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Western Blot: ISG15/UCRP Antibody (ARC53794) [NBP3-16129] - Analysis of extracts from wild type(WT) and ISG15 Rabbit mAb knockdown (KD) Hela(KD) cells, using ISG15 Rabbit mAb antibody at 1:510 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-16129_rabbit-isg15-ucrp-mab-arc53794-western-blot-31102023105530..jpg)
![ISG15/UCRP Antibody (ARC53794) [NBP3-16129] -Confocal imaging of U-2 OS cells using [KD Validated] ISG15 Rabbit mAb (dilution 1:100) (Red). The cells were counterstained with alpha -Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16129_rabbit-isg15-ucrp-mab-arc53794-immunocytochemistry-immunofluorescence-31102023105737..jpg)
![Immunocytochemistry/Immunofluorescence: METTL2A Antibody [NBP3-04709] - Analysis of NIH/3T3 cells using METTL2A Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16129_rabbit-isg15-ucrp-mab-arc53794-immunocytochemistry-immunofluorescence-31102023113040..jpg)
![Immunocytochemistry/Immunofluorescence: METTL2A Antibody [NBP3-04709] -Analysis of PC-12 cells using METTL2A Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16129_rabbit-isg15-ucrp-mab-arc53794-immunocytochemistry-immunofluorescence-31102023113159..jpg)
![Western Blot: ISG15/UCRP Antibody [NB600-891] - Immunoblot of hISG15 fusion protein. Anti-hISG15 antibody, generated by immunization with recombinant human ISG15, was tested by immunoblot against a hISG15-GFP fusion protein produced in E.coli cell lysate soluble fraction. Dilution of the antibody between 1:200 and 1:1,000 showed strong reactivity specifically with hISG15 and ISG15 coupled proteins. Free hISG15 is indicated by the arrowhead. In this blot the antibody was used at a 1:200 dilution incubated overnight at 4C in 5% non-fat dry milk in TTBS. Detection occurred using a 1:2000 dilution of HRP labeled Donkey anti-Rabbit IgG for 1h at room temperature. A chemiluminescence system was used for signal detection.](http://images.novusbio.com/fullsize/ISG15-UCRP-Antibody-Western-Blot-NB600-891-img0001.jpg "Western Blot: ISG15/UCRP Antibody [NB600-891] - Immunoblot of hISG15 fusion protein. Anti-hISG15 antibody, generated by immunization with recombinant human ISG15, was tested by immunoblot against a hISG15-GFP fusion protein produced in E.coli cell lysate soluble fraction. Dilution of the antibody between 1:200 and 1:1,000 showed strong reactivity specifically with hISG15 and ISG15 coupled proteins. Free hISG15 is indicated by the arrowhead. In this blot the antibody was used at a 1:200 dilution incubated overnight at 4C in 5% non-fat dry milk in TTBS. Detection occurred using a 1:2000 dilution of HRP labeled Donkey anti-Rabbit IgG for 1h at room temperature. A chemiluminescence system was used for signal detection.")
![N/A: ISG15/UCRP Antibody [NB600-891]](http://images.novusbio.com/fullsize/ISG15-UCRP-Antibody-N-A-NB600-891-img0002.jpg)
![Western Blot: ISG15/UCRP Antibody (3E5) [NBP1-04310] - Lane 1. : HeLa cell lysate Lane 2. : MCF7 cell lysate](http://images.novusbio.com/fullsize/ISG15-UCRP-Antibody-3E5-Western-Blot-NBP1-04310-img0003.jpg "Western Blot: ISG15/UCRP Antibody (3E5) [NBP1-04310] - Lane 1. : HeLa cell lysate Lane 2. : MCF7 cell lysate")
![Immunocytochemistry/Immunofluorescence: ISG15/UCRP Antibody (3E5) [NBP1-04310] - Analysis in HeLa cells line, stained with DAPI (Blue) for nucleus staining and with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/ISG15-UCRP-Antibody-3E5-Immunocytochemistry-Immunofluorescence-NBP1-04310-img0002.jpg)
![Western Blot: ISG15/UCRP Antibody (239) [NBP2-90146] - Lane A: Hela Whole Cell LysateLane B: 293 Whole Cell LysateLane C: Daudi Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:18 kDaObserved band size:15 kDa](http://images.novusbio.com/fullsize/ISG15-UCRP-Antibody-239-Western-Blot-NBP2-90146-img0002.jpg "Western Blot: ISG15/UCRP Antibody (239) [NBP2-90146] - Lane A: Hela Whole Cell LysateLane B: 293 Whole Cell LysateLane C: Daudi Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:18 kDaObserved band size:15 kDa")
![Immunocytochemistry/Immunofluorescence: ISG15/UCRP Antibody (239) [NBP2-90146] - Immunofluorescence staining of Human ProISG15 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human ProISG15 monoclonal antibody (1:60) at 37? 1 hour. Then cells were stained with the Alexa Fluo(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/ISG15-UCRP-Antibody-239-Immunocytochemistry-Immunofluorescence-NBP2-90146-img0001.jpg)
![Immunohistochemistry: ISG15/UCRP Antibody (16D8) [NBP3-26194] - Image of ISG15/UCRP Antibody (16D8) diluted at 1:100 and staining in paraffin-embedded human salivary gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26194_rabbit-isg15-ucrp-mab-16d8-262202415475646.jpg "Immunohistochemistry: ISG15/UCRP Antibody (16D8) [NBP3-26194] - Image of ISG15/UCRP Antibody (16D8) diluted at 1:100 and staining in paraffin-embedded human salivary gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.")
![Flow Cytometry: ISG15/UCRP Antibody (16D8) [NBP3-26194] - Overlay Peak curve showing HepG2 cells stained with ISG15/UCRP Antibody (16D8) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26194_rabbit-isg15-ucrp-mab-16d8-282202414312777.jpg)
![Western Blot: ISG15/UCRP Antibody (6C8) [NBP2-45437] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ISG15.](http://images.novusbio.com/fullsize/ISG15-UCRP-Antibody-6C8-Western-Blot-NBP2-45437-img0001.jpg "Western Blot: ISG15/UCRP Antibody (6C8) [NBP2-45437] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ISG15.")
![Western Blot: ISG15/UCRP Antibody (OTI6C8) - Azide and BSA Free [NBP2-71489] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ISG15.](http://images.novusbio.com/fullsize/ISG15-UCRP-Antibody-OTI6C8-Azide-and-BSA-Free-Western-Blot-NBP2-71489-img0001.jpg "Western Blot: ISG15/UCRP Antibody (OTI6C8) - Azide and BSA Free [NBP2-71489] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ISG15.")