Hu,
Mu,
Rt,
Po,
Dr,
Gt,
Ma,
Pm,
Pm,
Rb,
SyHaSpecies Glossary
Reactivity: Hu, Mu, Rt, Po, Dr, Gt, Ma, Pm, Pm, Rb, SyHa
WB, ELISA, Func, ICC/IF, IHC, IP, WB, ChIP, KD
Applications: WB, ELISA, Func, ICC/IF, IHC, IP, WB, ChIP, KD
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
![Immunohistochemistry: IRE1 alpha Antibody [NB100-2324] - XBP1 and TG2 deficiency promote increased IRE1a protein levels in cultured VSMCs and in neointima in situ. (D) Xbp1WT and Xbp1 delta WT and (E) Tgm2 WT and Tgm2 delta carotid artery sections were analyzed by IHC for total IRE1a. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0212235) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Immunohistochemistry-NB100-2324-img0010.jpg "Immunohistochemistry: IRE1 alpha Antibody [NB100-2324] - XBP1 and TG2 deficiency promote increased IRE1a protein levels in cultured VSMCs and in neointima in situ. (D) Xbp1WT and Xbp1 delta WT and (E) Tgm2 WT and Tgm2 delta carotid artery sections were analyzed by IHC for total IRE1a. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0212235) licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Western Blot: IRE1 alpha Antibody [NB100-2324] - Total IRE1 Alpha protein in lysates from Min6 cells which were transfected with GFP-siRNA or Ire1-siRNA. Theoretical molecular weight: 110 kDa.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Western-Blot-NB100-2324-img0006.jpg)
![Western Blot: IRE1 alpha Antibody [NB100-2324] - ALOX15 knockout blocks alcohol-induced up-regulation of IRE1a in mouse liver. Image from verified customer review.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Western-Blot-NB100-2324-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: IRE1 alpha Antibody [NB100-2324] - IRE-1 alpha antibody was tested in HeLa cells at 1:200 against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 568 (Red) and DAPI (Blue).](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2324-img0005.jpg)
![Immunohistochemistry-Paraffin: IRE1 alpha Antibody [NB100-2324] - Analysis of a formalin fixed mouse colon tissue section using total IRE1 alpha antibody (NB100-2324) at 1:300. Note a strong cytoplasmic signal in various cells of colonic villi (columnar epithelial and goblet cells) and the muscular layer. A few cells, potentially with active UPR, showed nuclear positivity also while the signal was negligible in cells at base of crypts and lamina propria.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-2324-img0007.jpg)
![Immunohistochemistry-Paraffin: IRE1 alpha Antibody [NB100-2324] - Analysis of a formalin fixed mouse colon tissue section using total IRE1 alpha antibody (NB100-2324) at 1:300. Note a strong cytoplasmic signal in various cells of colonic villi (columnar epithelial and goblet cells) and the muscular layer. Some cells, most likely with active UPR, depicted nuclear positivity also while the signal was negligible in crypts cells and lamina propria.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-2324-img0008.jpg)
![Western Blot: IRE1 alpha Antibody Pack [NBP2-50067] - Western Blot: IRE1 alpha [p Ser724] Antibody [NB100-2323] - Western blot of anti-IRE1 alpha (pSer724) using Lot AN and AO of NB100-2323. HeLa cells were treated (+) or untreated (-) with 10 mM DTT for 60 min to activate the UPR. Total protein was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% BSA in TBST. The membrane was probed with 2.0 ug/ml antibody in 5% BSA, and detected with an anti-rabbit HRP secondary antibody using chemiluminecence. Theoretical molecular weight: 110 kDa.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Pack-Western-Blot-NBP2-50067-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: IRE1 alpha Antibody Pack [NBP2-50067] - Western Blot: IRE1 alpha [p Ser724] Antibody [NB100-2323] - Western blot of anti-IRE1 alpha (pSer724) using Lot AN and AO of NB100-2323. HeLa cells were treated (+) or untreated (-) with 10 mM DTT for 60 min to activate the UPR. Total protein was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% BSA in TBST. The membrane was probed with 2.0 ug/ml antibody in 5% BSA, and detected with an anti-rabbit HRP secondary antibody using chemiluminecence. Theoretical molecular weight: 110 kDa.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: IRE1 alpha Antibody Pack [NBP2-50067] - P-Ire1a activation correlates with WFS1 cell expression. Cryosections of retina from 12 month old Wfs1+/+ and Wfs1-/- mouse were immunostained with anti-P-Ire1a antibody (red). DAPI was used for staining of cell nuclei (blue). RPE, retinal pigment epithelium; ONL, outer nuclear layer; INL, inner nuclear layer, RGC, retinal ganglion cells. Scale bars=50 um. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0097222) licensed under a CC-BY license. IRE1 alpha [p Ser724] Antibody [NB100-2323]](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Pack-Immunohistochemistry-NBP2-50067-img0006.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: IRE1 alpha Antibody Pack [NBP2-50067] - Western Blot detection of Total IRE1 Alpha in transfected Min6 cells using IRE1 alpha Antibody [NB100-2324]](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Pack-Western-Blot-NBP2-50067-img0002.jpg)
![Immunohistochemistry: IRE1 alpha Antibody Pack [NBP2-50067] - XBP1 and TG2 deficiency promote increased IRE1a protein levels in cultured VSMCs and in neointima in situ. (D) Xbp1WT and Xbp1 delta WT and (E) Tgm2 WT and Tgm2 delta carotid artery sections were analyzed by IHC for total IRE1a. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0212235) licensed under a CC-BY license. IRE1 alpha Antibody [NB100-2324]](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Pack-Immunohistochemistry-NBP2-50067-img0008.jpg)
![Western Blot: IRE1 alpha Antibody [NBP2-95252] - Analysis of extracts of various cell lines, using IRE1 alpha antibody (NBP2-95252) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Western-Blot-NBP2-95252-img0008.jpg "Western Blot: IRE1 alpha Antibody [NBP2-95252] - Analysis of extracts of various cell lines, using IRE1 alpha antibody (NBP2-95252) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: IRE1 alpha Antibody [NBP2-95252] - Immunofluorescence analysis of PC-12 cells using IRE1 alpha Rabbit pAb (NBP2-95252) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-95252-img0003.jpg)
![Immunohistochemistry-Paraffin: IRE1 alpha Antibody [NBP2-95252] - Rat pancreas using IRE1 alpha Rabbit pAb (NBP2-95252) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-95252-img0007.jpg)
![Western Blot: IRE1 alpha Antibody [NBP2-95252] - Analysis of extracts of 293T cells, using IRE1 alpha antibody (NBP2-95252) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 120s.](http://images.novusbio.com/fullsize/IRE1 alpha Antibody-Western Blot-NBP2-95252-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: IRE1 alpha Antibody [NBP2-95252] - Immunofluorescence analysis of U2OS cells using IRE1 alpha Rabbit pAb (NBP2-95252) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-95252-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: IRE1 alpha Antibody [NBP2-95252] - Immunofluorescence analysis of NIH/3T3 cells using IRE1 alpha Rabbit pAb (NBP2-95252) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-95252-img0002.jpg)
![Immunohistochemistry-Paraffin: IRE1 alpha Antibody [NBP2-95252] - Human breast cancer using IRE1 alpha Rabbit pAb (NBP2-95252) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-95252-img0004.jpg)
![Immunohistochemistry-Paraffin: IRE1 alpha Antibody [NBP2-95252] - Mouse kidney using IRE1 alpha Rabbit pAb (NBP2-95252) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-95252-img0005.jpg)
![Immunohistochemistry-Paraffin: IRE1 alpha Antibody [NBP2-95252] - Rat kidney using IRE1 alpha Rabbit pAb (NBP2-95252) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-95252-img0006.jpg)
![Western Blot: IRE1 alpha Antibody (8D12) [H00002081-M02] - IRE1 Antibody (8D12) [H00002081-M02] - ERN1 monoclonal antibody (M02), clone 8D12. Analysis of ERN1 expression in LNCaP. Theoretical molecular weight: 110 kDa.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-8D12-Western-Blot-H00002081-M02-img0008.jpg "Western Blot: IRE1 alpha Antibody (8D12) [H00002081-M02] - IRE1 Antibody (8D12) [H00002081-M02] - ERN1 monoclonal antibody (M02), clone 8D12. Analysis of ERN1 expression in LNCaP. Theoretical molecular weight: 110 kDa.")
![Immunocytochemistry/Immunofluorescence: IRE1 alpha Antibody (8D12) [H00002081-M02] - Analysis of monoclonal antibody to ERN1 on HeLa cell. Antibody concentration 25 ug/ml.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-8D12-Immunocytochemistry-Immunofluorescence-H00002081-M02-img0006.jpg)
![Western Blot: IRE1 alpha Antibody (8D12) [H00002081-M02] - Analysis of ERN1 expression in HeLa. Theoretical molecular weight: 110 kDa. Observed molecular weight may vary.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-8D12-Western-Blot-H00002081-M02-img0001.jpg)
![Proximity Ligation Assay: IRE1 alpha Antibody (8D12) [H00002081-M02] - Analysis of protein-protein interactions between TRAF2 and ERN1. HeLa cells were stained with anti-TRAF2 rabbit purified polyclonal 1:1200 and anti-ERN1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-8D12-Proximity-Ligation-Assay-H00002081-M02-img0004.jpg)
![ELISA: IRE1 alpha Antibody (8D12) [H00002081-M02] - IRE1 Antibody (8D12) [H00002081-M02] - Detection limit for recombinant GST tagged ERN1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-8D12-ELISA-H00002081-M02-img0007.jpg)
![Western Blot: IRE1 alpha [p Ser724] Antibody [NBP3-12124] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with IRE1 alpha (phospho Ser724) antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/IRE1-alpha-[p-Ser724]-Antibody-Western-Blot-NBP3-12124-img0006.jpg "Western Blot: IRE1 alpha [p Ser724] Antibody [NBP3-12124] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with IRE1 alpha (phospho Ser724) antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: IRE1 alpha [p Ser724] Antibody [NBP3-12124] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IRE1 alpha (phospho Ser724) antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/IRE1-alpha-[p-Ser724]-Antibody-Western-Blot-NBP3-12124-img0002.jpg)
![Western Blot: IRE1 alpha [p Ser724] Antibody [NBP3-12124] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IRE1 alpha (phospho Ser724) antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/IRE1-alpha-[p-Ser724]-Antibody-Western-Blot-NBP3-12124-img0004.jpg)
![Western Blot: IRE1 alpha [p Ser724] Antibody [NBP3-12124] -Untreated (-) and treated (+) Neuro2A whole cell extract (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with IRE1 alpha (phospho Ser724) antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301)) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/antibody/nbp3-12124_rabbit-polyclonal-ire1-alpha-p-ser724-antibody-western-blot-41202413936..jpg)
![Immunohistochemistry-Paraffin: IRE1 alpha Antibody (10B2B2) [NBP3-05787] - Staining of human ERN1 in human epididymis with mouse monoclonal antibody (1:100).](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-10B2B2-Immunohistochemistry-Paraffin-NBP3-05787-img0003.jpg "Immunohistochemistry-Paraffin: IRE1 alpha Antibody (10B2B2) [NBP3-05787] - Staining of human ERN1 in human epididymis with mouse monoclonal antibody (1:100).")
![Immunohistochemistry-Paraffin: IRE1 alpha Antibody (10B2B2) [NBP3-05787] - Staining of human ERN1 in human liver with mouse monoclonal antibody (1:300).](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-10B2B2-Immunohistochemistry-Paraffin-NBP3-05787-img0001.jpg)
![Immunohistochemistry-Paraffin: IRE1 alpha Antibody (10B2B2) [NBP3-05787] - Staining of human ERN1 in human tonsil with mouse monoclonal antibody (1:300).](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-10B2B2-Immunohistochemistry-Paraffin-NBP3-05787-img0002.jpg)
![SDS-Page: Recombinant Human IRE1 alpha Protein [H00002081-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00002081-P01-1.jpg "SDS-Page: Recombinant Human IRE1 alpha Protein [H00002081-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: IRE1 alpha [p Ser724] Antibody (1F12) [NBP3-26425] - Positive Western Blot detected in: 293 whole cell lysate, A549 whole cell lysate, Hela whole cell lysate (treated with Calyculin A or EGF). All lanes: IRE1 alpha [p Ser724] at 0.75 ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 110 kDa Observed band size: 110 kDa](http://images.novusbio.com/fullsize/nbp3-26425_rabbit-ire1-alpha-p-ser-724-mab-1f12-28220241147219.jpg "Western Blot: IRE1 alpha [p Ser724] Antibody (1F12) [NBP3-26425] - Positive Western Blot detected in: 293 whole cell lysate, A549 whole cell lysate, Hela whole cell lysate (treated with Calyculin A or EGF). All lanes: IRE1 alpha [p Ser724] at 0.75 ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 110 kDa Observed band size: 110 kDa")
![Immunocytochemistry/Immunofluorescence: IRE1 alpha [p Ser724] Antibody (1F12) [NBP3-26425] - Staining of Hela cells with IRE1 alpha [p Ser724] Antibody (1F12) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26425_rabbit-ire1-alpha-p-ser-724-mab-1f12-282202412475125.jpg)