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![Flow Cytometry: HLA G Antibody (87G) [NBP1-43123] - An intracellular stain was performed on Hek293 cells with HLA G Antibody [87G] NBP1-43123AF488 (blue) and a matched isotype control (orange). Both antibodies were conjugated to Alexa Fluor 488. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature.](http://images.novusbio.com/fullsize/HLA-G-Antibody-87G-Flow-Cytometry-NBP1-43123-img0008.jpg "Flow Cytometry: HLA G Antibody (87G) [NBP1-43123] - An intracellular stain was performed on Hek293 cells with HLA G Antibody [87G] NBP1-43123AF488 (blue) and a matched isotype control (orange). Both antibodies were conjugated to Alexa Fluor 488. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature.")
![Immunocytochemistry/Immunofluorescence: HLA G Antibody (87G) [NBP1-43123] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti HLA G (87G) at a 1:200 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/HLA-G-Antibody-87G-Immunocytochemistry-Immunofluorescence-NBP1-43123-img0006.jpg)
![Flow Cytometry: HLA G Antibody (87G) [NBP1-43123] - An intracellular stain was performed on HeLa cells with HLA G [87G] Antibody NBP1-43123AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/HLA-G-Antibody-87G-Flow-Cytometry-NBP1-43123-img0009.jpg)
![Flow Cytometry: HLA G Antibody (87G) [NBP1-43123] - Staining of stimulated U937 cells with 0.25 ug of Mouse IgG2a K Isotype Control Purified (blue) or 0.25 ug of Anti-Human HLA-G Purified (purple) followed by F(ab')2 Anti-Mouse IgG PE. Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/HLA-G-Antibody-87G-Flow-Cytometry-NBP1-43123-img0002.jpg)
![Flow Cytometry: HLA G Antibody (87G) [NBP1-43123] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using HLA G antibody (dark blue) and isotype control (orange). An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/HLA-G-Antibody-87G-Flow-Cytometry-NBP1-43123-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: HLA G Antibody (MEM-G/9) [NB500-314] - Staining of HLA-G1 transfectants (LCL-HLA-G1) using Alexa Fluor 488 conjugated anti-human HLA-G (MEM-G/9).](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-9-Immunocytochemistry-Immunofluorescence-NB500-314-img0006.jpg "Immunocytochemistry/Immunofluorescence: HLA G Antibody (MEM-G/9) [NB500-314] - Staining of HLA-G1 transfectants (LCL-HLA-G1) using Alexa Fluor 488 conjugated anti-human HLA-G (MEM-G/9).")
![Flow Cytometry: HLA G Antibody (MEM-G/9) [NB500-314] - Surface staining of HLA-G1 transfectants (LCL-HLA-G1) using anti-HLA-G (MEM-G/9) PE.](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-9-Flow-Cytometry-NB500-314-img0012.jpg)
![Flow Cytometry: HLA G Antibody (MEM-G/9) [NB500-314] - Analysis of APC conjugate of NB500-314. Double surface staining of HLA-G1 transfectants (viable cells gate) using anti-human HLA-G (MEM-G/9) APC and anti-human HLA-G (MEM-G/11) FITC.](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-9-Flow-Cytometry-NB500-314-img0010.jpg)
![Flow Cytometry: HLA G Antibody (MEM-G/9) [NB500-314] - Surface staining of HLA-G1 transfectants (LCL-HLA-G1) using anti-HLA-G (MEM-G/9) FITC.](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-9-Flow-Cytometry-NB500-314-img0011.jpg)
![Immunoprecipitation: HLA G Antibody (MEM-G/9) [NB500-314] - Immunoprecipitation of HLA-G from HLA-G1 transfectanted lysates (LCL-HLA-G1) by anti-human HLA-G (MEM-G/9, NB500-314) and protein G. HLA-G was detected by anti-human HLA-G (4H84) and goat anti-mouse HRP in cell lysate (Lanes 1) and in the immunoprecipitate (Lanes 2).](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-9-Immunoprecipitation-NB500-314-img0009.jpg)
![Immunohistochemistry: HLA G Antibody (MEM-G/1) [NB500-302] - ELA affects first trimester placental explant trophoblast proliferation. HLA-G immunostaining (brown color) indicates the location of extravillous trophoblasts. In control explants (left panel) almost all villous cytotrophoblasts proliferate, while this is much less apparent in explants treated with ELA (0.25nM, right panel). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-55650-5) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-1-Immunohistochemistry-NB500-302-img0005.jpg "Immunohistochemistry: HLA G Antibody (MEM-G/1) [NB500-302] - ELA affects first trimester placental explant trophoblast proliferation. HLA-G immunostaining (brown color) indicates the location of extravillous trophoblasts. In control explants (left panel) almost all villous cytotrophoblasts proliferate, while this is much less apparent in explants treated with ELA (0.25nM, right panel). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-55650-5) licensed under a CC-BY license.")
![Western Blot: HLA G Antibody (MEM-G/1) [NB500-302] - Using the Biotin direct conjugate analysis (reducing conditions) of HLA-G1 in HLA-G1 transfectants using the antibody MEM-G/1 biotin.](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-1-Western-Blot-NB500-302-img0003.jpg)
![Immunohistochemistry: HLA G Antibody (MEM-G/1) [NB500-302] - Fig. 1B - first-trimester placenta (paraffin-embedded sections)](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-1-Immunohistochemistry-NB500-302-img0001.jpg)
![Immunohistochemistry: HLA G Antibody (MEM-G/1) [NB500-302] - Fig. 1. Immunohistochemistry staining with anti-human HLA-G (MEM-G/1). Fig. 1A - pulmonary disseases (paraffin-embedded sections) The antibody MEM-G/1 stains infiltrating macrophages in pulmonary diseases. In the top left corner see the detail of macrophage.](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-1-Immunohistochemistry-NB500-302-img0002.jpg)
![Flow Cytometry: HLA G Antibody (01G) - BSA Free [NBP1-44926] - Separation of HLA-G transfected LCL cells (red-filled) from K562 cells (black-dashed) in flow cytometry analysis (surface staining) using anti-human HLA-G (01G) purified antibody (concentration in sample 16 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/HLA-G-Antibody-01G-BSA-Free-Flow-Cytometry-NBP1-44926-img0004.jpg "Flow Cytometry: HLA G Antibody (01G) - BSA Free [NBP1-44926] - Separation of HLA-G transfected LCL cells (red-filled) from K562 cells (black-dashed) in flow cytometry analysis (surface staining) using anti-human HLA-G (01G) purified antibody (concentration in sample 16 ug/ml) GAM APC.")
![Flow Cytometry: HLA G Antibody (01G) [NBP1-44926] - Surface staining of HLA-G transfected LCL cells with anti-HLA-G (01G) purified, GAM-APC.](http://images.novusbio.com/fullsize/HLA-G-Antibody-01G-Flow-Cytometry-NBP1-44926-img0001.jpg)
![Flow Cytometry: HLA G Antibody (01G) [NBP1-44926] - Surface staining of HLA-G transfected LCL cells with anti-HLA-G (01G) FITC.](http://images.novusbio.com/fullsize/HLA-G-Antibody-01G-Flow-Cytometry-NBP1-44926-img0002.jpg)
![Flow Cytometry: HLA G Antibody (01G) - BSA Free [NBP1-44926] - Surface staining pattern of HLA-G transfected LCL cells using anti-human HLA-G (01G) purified antibody (concentration in sample 16 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/HLA-G-Antibody-01G-BSA-Free-Flow-Cytometry-NBP1-44926-img0003.jpg)
![Immunohistochemistry-Paraffin: HLA G Antibody (4H84) [NB110-55297] - Staining of human spleen (paraffin sections) using anti-HLA-G (4H84).](http://images.novusbio.com/fullsize/HLA-G-Antibody-4H84-Immunohistochemistry-Paraffin-NB110-55297-img0003.jpg "Immunohistochemistry-Paraffin: HLA G Antibody (4H84) [NB110-55297] - Staining of human spleen (paraffin sections) using anti-HLA-G (4H84).")
![Western Blot: HLA G Antibody (4H84) [NB110-55297] - Analysis of HLA-G in HLA-G1 transfectants (LCL-HLA-G1) by 4H84 antibody-biotin.](http://images.novusbio.com/fullsize/HLA-G-Antibody-4H84-Western-Blot-NB110-55297-img0002.jpg)
![Western Blot: HLA G Antibody (4H84) [NB110-55297] - HLA G Antibody (4h84) [NB110-55297] - Immunoprecipitation of HLA-G from HLA-G1 transfectanted lysates (LCL-HLA-G1) by MEM-G/9 antibody and protein G. HLA-G was detected by 4H84 antibody (NB110-55297) and goat anti-mouse-HRP conjugate in cell lysate (Lane 1) and in the immunoprecipitate (Lane 2).](http://images.novusbio.com/fullsize/HLA-G-Antibody-4h84-Immunoprecipitation-NB110-55297-img0001.jpg)
![Western Blot: HLA G Antibody (5A6G7) [NBP1-44924] - Analysis of whole cell lysate of HLA-G stable transfectants (various splice variants) using anti-human HLA-G (5A6G7). Lane 1: M8 cell line transfected with empty vector Lane 2: M8 cell line transfected with HLA-G1 Lane 3: M8 cell line transfected with HLA-G5 Lane 4: M8 cell line transfected with HLA-G6](http://images.novusbio.com/fullsize/HLA-G-Antibody-5A6G7-Western-Blot-NBP1-44924-img0001.jpg "Western Blot: HLA G Antibody (5A6G7) [NBP1-44924] - Analysis of whole cell lysate of HLA-G stable transfectants (various splice variants) using anti-human HLA-G (5A6G7). Lane 1: M8 cell line transfected with empty vector Lane 2: M8 cell line transfected with HLA-G1 Lane 3: M8 cell line transfected with HLA-G5 Lane 4: M8 cell line transfected with HLA-G6")
![Flow Cytometry: HLA G Antibody (5A6G7) [NBP1-44924] - Staining of HLA-G in HLA-G1 transfectants with anti-HLA-G (5A6G7) biotin / streptavidin-APC.](http://images.novusbio.com/fullsize/HLA-G-Antibody-5A6G7-Flow-Cytometry-NBP1-44924-img0003.jpg)
![Flow Cytometry: HLA G Antibody (5A6G7) [NBP1-44924] - Staining of HLA-G in HLA-G6 transfectants with anti-HLA-G (5A6G7) PE.](http://images.novusbio.com/fullsize/HLA-G-Antibody-5A6G7-Flow-Cytometry-NBP1-44924-img0002.jpg)
![Flow Cytometry: HLA G Antibody (2A12) [NBP1-44925] - Surface staining of HLA-G (on transfected cells, red and orange) with anti-HLA-G (2A12) FITC, compared with nontransfected cells (blue, green).](http://images.novusbio.com/fullsize/HLA-G-Antibody-2A12-Flow-Cytometry-NBP1-44925-img0002.jpg "Flow Cytometry: HLA G Antibody (2A12) [NBP1-44925] - Surface staining of HLA-G (on transfected cells, red and orange) with anti-HLA-G (2A12) FITC, compared with nontransfected cells (blue, green).")
![Flow Cytometry: HLA G Antibody (2A12) [NBP1-44925] - Surface staining of HLA-G (on transfected cells) with anti-HLA-G (2A12) purified, GAM-APC.](http://images.novusbio.com/fullsize/HLA-G-Antibody-2A12-Flow-Cytometry-NBP1-44925-img0001.jpg)
![Western Blot: HLA G Antibody [NBP2-16855] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HLA-G antibody diluted at 1:500.](http://images.novusbio.com/fullsize/HLA-G-Antibody-Western-Blot-NBP2-16855-img0005.jpg "Western Blot: HLA G Antibody [NBP2-16855] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HLA-G antibody diluted at 1:500.")
![Immunohistochemistry-Paraffin: HLA G Antibody [NBP2-16855] - Human lung cancer. HLA-G antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/HLA-G-Antibody-Immunohistochemistry-Paraffin-NBP2-16855-img0003.jpg)
![Immunohistochemistry-Paraffin: HLA G Antibody [NBP2-16855] - Mahlarvu xenograft, using HLA-G antibody at 1:500 dilution. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min](http://images.novusbio.com/fullsize/HLA-G-Antibody-Immunohistochemistry-Paraffin-NBP2-16855-img0001.jpg)
![Immunohistochemistry-Paraffin: HLA G Antibody [NBP2-16855] - Human endometrial cancer. HLA-G antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/HLA-G-Antibody-Immunohistochemistry-Paraffin-NBP2-16855-img0002.jpg)
![Western Blot: HLA G Antibody (MEM-G/4) [NB500-533] - Analysis of HLA-G by the antibody MEM-G/4 on HLA-G1 transfectants (LCL-HLA-G1).](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-4-Western-Blot-NB500-533-img0001.jpg "Western Blot: HLA G Antibody (MEM-G/4) [NB500-533] - Analysis of HLA-G by the antibody MEM-G/4 on HLA-G1 transfectants (LCL-HLA-G1).")
![Flow Cytometry: HLA G Antibody (G233) [NB110-55298] - Surface staining of HLA-G in HLA-G transfectants using anti-HLA-G (G233) purified / GAM-APC.](http://images.novusbio.com/fullsize/HLA-G-Antibody-G233-Flow-Cytometry-NB110-55298-img0001.jpg "Flow Cytometry: HLA G Antibody (G233) [NB110-55298] - Surface staining of HLA-G in HLA-G transfectants using anti-HLA-G (G233) purified / GAM-APC.")
![SDS-Page: Recombinant Human HLA G Protein [NBP2-61457] - 15% SDS-Page (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-Hemoglobin-zeta-Protein-SDS-Page-NBP2-61457-img0001.jpg "SDS-Page: Recombinant Human HLA G Protein [NBP2-61457] - 15% SDS-Page (3ug)")