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110 Results for "novus"
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AF231

EGFR Antibody [Unconjugated]

(30 Publications)
100 μg
AF231
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MDA‑MB‑231 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EGFR at approximately 175 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu
Species Glossary
Reactivity: Hu
WB, Simple Western, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ICC/IF
Applications: WB, Simple Western, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ICC/IF
Goat Polyclonal
Host: Goat Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Biotin BAF231
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF231-SP
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AF1280

EGFR Antibody [Unconjugated]

(1 Review)
(10 Publications)
100 μg
AF1280
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MDA‑MB‑231 human breast cancer cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1280) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for EGFR at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Mu
Species Glossary
Reactivity: Mu
WB, Simple Western, IHC
Applications: WB, Simple Western, IHC
Goat Polyclonal
Host: Goat Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Biotin BAF1280
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF1280-SP
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AF1095

EGFR [p Tyr1173] Antibody [Unconjugated]

(10 Publications)
50 μg
AF1095
Western blot shows lysates of A431 human epithelial carcinoma cell line untreated(‑) or treated (+) with 100 μM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human Phospho-EGFR (Y1173) Antigen Affinity-purified Polyclonal Antibody, followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-EGFR (Y1173) at approximately 185 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu
Species Glossary
Reactivity: Hu
WB, Simple Western, IHC, CyTOF-ready, Flow, ICC/IF
Applications: WB, Simple Western, IHC, CyTOF-ready, Flow, ICC/IF
Rabbit Polyclonal
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF1095-SP
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DYC1095B-2

Human Phospho-EGFR DuoSet IC ELISA, 2 Plate

(14 Publications)
1 Kit (for 2 Plates)
DYC1095B-2
The human epidermoid carcinoma cell line, A431, was treated with 25 ng/mL recombinant human EGF (R&D Systems Catalog #236-EG) for 5 minutes to induce tyrosine phosphorylation of EGF R. Lysates were serially diluted and analyzed by (A) IP-Western blot and (B) this DuoSet IC ELISA. IPs were done using an anti-EGF R monoclonal antibody and anti-mouse IgG agarose. Immunoblots were incubated with an HRP-conjugated anti-phospho-tyrosine monoclonal antibody (R&D Systems, Catalog #HAM1676) to detect phosphor-EGF R. Bands were visualized by chemiluminescent detection. Human Phospho-EGF R can be detected in this DuoSet IC ELISA by using approximately 2-4 times less lysate than is needed for a conventional IP-Western blot.
Hu
Species Glossary
Reactivity: Hu
ELISA
Applications: ELISA
Conjugate Catalog # Availability Size Price
Biotin DYC1095BE
Biotin DYC1095B-5
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free DYC1095BE
Carrier Free DYC1095B-5
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MAB3570

EGFR [p Tyr1068] Antibody (338324) [Unconjugated]

(1 Review)
(9 Publications)
100 μg
MAB3570
Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human EGF (Catalog # 236-EG) for 5 minutes. PVDF membrane was probed with 1 µg/mL of Human Phospho-EGFR (Y1068) Monoclonal Antibody (Catalog # MAB3570), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-EGFR (Y1068) at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu
Species Glossary
Reactivity: Hu
WB, CyTOF-ready, Flow
Applications: WB, CyTOF-ready, Flow
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 FAB3570U
Alexa Fluor 350 IC3570U-100UG
Alexa Fluor 405 FAB3570V
Alexa Fluor 405 IC3570V-100UG
Alexa Fluor 488 IC3570G-100UG
Alexa Fluor 488 FAB3570G
Alexa Fluor 532 FAB3570X
Alexa Fluor 594 IC3570T-100UG
Alexa Fluor 594 FAB3570T
Alexa Fluor 647 FAB3570R
Alexa Fluor 647 IC3570R-100UG
Alexa Fluor 700 IC3570N-100UG
Alexa Fluor 700 FAB3570N
Alexa Fluor 750 FAB3570S
Alexa Fluor 750 IC3570S-100UG
Allophycocyanin FAB3570A
Biotin FAB3570B
DyLight 350 FAB3570D
DyLight 405 FAB3570E
DyLight 488 FAB3570K
DyLight 550 FAB3570L
DyLight 594 FAB3570M
DyLight 650 FAB3570W
DyLight 680 FAB3570Y
DyLight 755 FAB3570Z
FITC FAB3570F
Fluorescein IC3570F
HRP FAB3570H
Janelia Fluor 549 FAB3570I
Janelia Fluor 646 FAB3570J
PE FAB3570P
PerCP FAB3570C
mFluor Violet 450 SE FAB3570MFV450
mFluor Violet 500 SE FAB3570MFV500
mFluor Violet 610 SE FAB3570MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free IC3570F
Carrier Free MAB3570-SP
Carrier Free IC3570T-100UG
Carrier Free IC3570S-100UG
Carrier Free IC3570R-100UG
Carrier Free IC3570N-100UG
Carrier Free IC3570G-100UG
Carrier Free IC3570V-100UG
Carrier Free IC3570U-100UG
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344-ER

Recombinant Human EGFR Fc Chimera Protein, CF

(14 Publications)
50 μg
344-ER
Recombinant human EGFR Fc chimera (344-ER) binds recombinant human EGF (236-EG) in a functional ELISA. The estimated Kd for this interaction is < 8 nM.
Hu
Species Glossary
Reactivity: Hu
Binding Activity
Applications: Binding Activity
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NBP2-50599

EGFR Antibody (DH8.3) - EGFRvIII, Mutant - BSA Free

(8 Publications)
0.1 ml
NBP2-50599
Flow Cytometry: EGFR Antibody (DH8.3) - EGFRvIII [NBP2-50599] - An intracellular stain was performed on A431 cells with EGFR [DH8.3] Antibody NBP2-50599R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.
Hu
Species Glossary
Reactivity: Hu
WB, ELISA, Flow, IHC, IP, CyTOF-ready
Applications: WB, ELISA, Flow, IHC, IP, CyTOF-ready
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 NBP2-50599AF350
Alexa Fluor 405 NBP2-50599AF405
Alexa Fluor 488 NBP2-50599AF488
Alexa Fluor 532 NBP2-50599AF532
Alexa Fluor 594 NBP2-50599AF594
Alexa Fluor 647 NBP2-50599AF647
Alexa Fluor 700 NBP2-50599AF700
Alexa Fluor 750 NBP2-50599AF750
Allophycocyanin NBP2-50599APC
Allophycocyanin/Cy7 NBP2-50599APCCY7
Biotin NBP2-50599B
DyLight 350 NBP2-50599UV
DyLight 405 NBP2-50599V
DyLight 488 NBP2-50599G
DyLight 550 NBP2-50599R
DyLight 594 NBP2-50599DL594
DyLight 650 NBP2-50599C
DyLight 680 NBP2-50599FR
DyLight 755 NBP2-50599IR
FITC NBP2-50599F
HRP NBP2-50599H
Janelia Fluor 549 NBP2-50599JF549
Janelia Fluor 646 NBP2-50599JF646
PE NBP2-50599PE
PE/Atto594 NBP2-50599PEATT594
PE/Cy5.5 NBP2-50599PECY55
PE/Cy7 NBP2-50599PECY7
PerCP NBP2-50599PCP
mFluor Violet 450 SE NBP2-50599MFV450
mFluor Violet 500 SE NBP2-50599MFV500
mFluor Violet 610 SE NBP2-50599MFV610
Available Formulations
Formulation Catalog # Availability Price  
BSA Free NBP2-50599-0.025ml
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DYC1854-2

Human Total EGFR DuoSet IC ELISA, 2 Plate

(9 Publications)
1 Kit (for 2 Plates)
DYC1854-2
ELISA EGFR [Biotin]
Hu
Species Glossary
Reactivity: Hu
ELISA
Applications: ELISA
Conjugate Catalog # Availability Size Price
Biotin DYC1854E
Biotin DYC1854-5
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NBP1-84814

EGFR Antibody

(3 Publications)
0.1 ml
NBP1-84814
Immunohistochemistry-Paraffin: EGFR Antibody [NBP1-84814] - Staining in human placenta and tonsil tissues using anti-EGFR antibody. Corresponding EGFR RNA-seq data are presented for the same tissues.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, ICC/IF, IHC
Applications: WB, ICC/IF, IHC
Rabbit Polyclonal
Host: Rabbit Polyclonal
Unconjugated  
         
Validated by:          
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AF3394

EGFR [p Tyr845] Antibody [Unconjugated]

(4 Publications)
50 μg
AF3394
Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human EGF (Catalog # 236-EG) for 5 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-EGFR (Y845) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3394), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-EGFR (Y845) at approximately 175 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu
Species Glossary
Reactivity: Hu
WB, IHC, CyTOF-ready, Flow, ICC/IF
Applications: WB, IHC, CyTOF-ready, Flow, ICC/IF
Rabbit Polyclonal
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF3394-SP
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MAB1095

EGFR Antibody (102618) [Unconjugated]

(9 Publications)
100 μg
Hu
Species Glossary
Reactivity: Hu
WB
Applications: WB
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB1095-SP
Carrier Free MAB1095-500
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DEGFR0

Human EGFR Quantikine ELISA Kit

(4 Publications)
1 Kit
DEGFR0
N/A EGFR [HRP]
Hu
Species Glossary
Reactivity: Hu
ELISA
Applications: ELISA
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