Hu,
Mu,
Rt,
Po,
Bv,
ShSpecies Glossary
Reactivity: Hu, Mu, Rt, Po, Bv, Sh
WB, Simple Western, Flow, ICC/IF, IHC, IP, ChIP, CyTOF-ready, IHC, KD
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IP, ChIP, CyTOF-ready, IHC, KD
Host: Mouse Monoclonal
Alt Catalog# IMG-261A
Sample Size Available
Sizes: Sample Size Available
![Western Blot: DNMT1 Antibody (60B1220.1) [NB100-56519] - Analysis of Dnmt1 in 2102EP (human embryonic carcinoma) in the A) absence and B) presence of immunizing peptide, recombinant human Dnmt1 protein in the C) absence and D) presence of immunizing peptide, NIH 3T3 (mouse embryonic fribroblast) in the E) absence and F) presence of immunizing peptide, and D3 (mouse embryonic stem cell) in the G) absence and H) presence of immunizing peptide using Dnmt1 antibody. this antibody. 5 ug/ml for testing the human cell line and 0.1 ug/ml was used for testing the recombinant protein and the mouse cell lines. ’s goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/DNMT1-Antibody-60B1220-1-Western-Blot-NB100-56519-img0003.jpg "Western Blot: DNMT1 Antibody (60B1220.1) [NB100-56519] - Analysis of Dnmt1 in 2102EP (human embryonic carcinoma) in the A) absence and B) presence of immunizing peptide, recombinant human Dnmt1 protein in the C) absence and D) presence of immunizing peptide, NIH 3T3 (mouse embryonic fribroblast) in the E) absence and F) presence of immunizing peptide, and D3 (mouse embryonic stem cell) in the G) absence and H) presence of immunizing peptide using Dnmt1 antibody. this antibody. 5 ug/ml for testing the human cell line and 0.1 ug/ml was used for testing the recombinant protein and the mouse cell lines. ’s goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunocytochemistry/Immunofluorescence: DNMT1 Antibody (60B1220.1) [NB100-56519] - Dnmt1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Antibody dilution of 1:10 was used. Image objective 40x.](http://images.novusbio.com/fullsize/DNMT1-Antibody-60B1220-1-Immunocytochemistry-Immunofluorescence-NB100-56519-img0006.jpg)
![Immunohistochemistry-Paraffin: DNMT1 Antibody (60B1220.1) [NB100-56519] - Tissue section of human hepatocellular carcinoma at 1:50 dilution.](http://images.novusbio.com/fullsize/DNMT1-Antibody-60B1220-1-Immunohistochemistry-Paraffin-NB100-56519-img0007.jpg)
![Flow Cytometry: DNMT1 Antibody (60B1220.1) [NB100-56519] - An intracellular stain was performed on HeLa cells with DNMT1 [60B1220.1] Antibody NB100-56519AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/DNMT1-Antibody-60B1220-1-Flow-Cytometry-NB100-56519-img0010.jpg)
![Flow Cytometry: DNMT1 Antibody (60B1220.1) [NB100-56519] - Analysis of PE conjugate of NB100-56519. An intracellular stain was performed on HeLa cells with DNMT1 antibody (60B1220.1) NB100-56519PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with 0](http://images.novusbio.com/fullsize/DNMT1-Antibody-60B1220-1-Flow-Cytometry-NB100-56519-img0009.jpg)
![Simple Western: DNMT1 Antibody (60B1220.1) [NB100-56519] - Simple Western lane view shows a specific band for Dnmt1 in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/DNMT1-Antibody-60B1220-1-Simple-Western-NB100-56519-img0008.jpg)
![Immunoprecipitation: DNMT1 Antibody (60B1220.1) [NB100-56519] - The PCNAS228I mutation differentially affects PCNA binding to PIP box-containing proteins. GST-PIP pull down of His-S-PCNAWT or PCNAS228I. Figure shows Coomassie stained gel of representative pull down (top) and anti-PCNA western blot of the same samples diluted 1:20 (bottom). Amount of 'input' loaded for Coomassie is equivalent to 1%, 'Glut. beads' (Glutathione sepharose 4B beads) is equivalent to 25%. Molecular weight markers are indicated. Image collected and cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S1568786416304013), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DNMT1-Antibody-60B1220-1-Immunoprecipitation-NB100-56519-img0011.jpg)
![Western Blot: DNMT1 Antibody [NB100-392] - Detection of human DNMT1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DNMT1 antibody NB100-392 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/DNMT1-Antibody-Western-Blot-NB100-392-img0019.jpg "Western Blot: DNMT1 Antibody [NB100-392] - Detection of human DNMT1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DNMT1 antibody NB100-392 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: DNMT1 Antibody [NB100-392] - Dnmt1 in mouse brain cells. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/DNMT1-Antibody-Western-Blot-NB100-392-img0002.jpg)
![Immunoprecipitation: DNMT1 Antibody [NB100-392] - Detection of human DNMT1 in HeLa nuclear extracts [50 ug/lane (A), 10 ug input, or 1 mg NB 100-392 IP (B)] using NB 100-392. DNMT1 immunoprecipitated with NB 100-392 and detected with NB 100-392 using 0.2 ug/ml. 2 second ECL exposure.](http://images.novusbio.com/fullsize/DNMT1-Antibody-Immunoprecipitation-NB100-392-img0004.jpg)
![Immunoprecipitation: DNMT1 Antibody [NB100-392] - Detection of human DNMT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DNMT1 antibody NB100-392 used for IP at 3 ug per reaction. For blotting immunoprecipitated DNMT1, NB100-392 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/DNMT1-Antibody-Immunoprecipitation-NB100-392-img0020.jpg)
![Western Blot: DNMT1 Antibody (8E3H1) [NBP3-15856] - Western blot analysis of extracts of various cell lines, using DNMT1 antibody (NBP3-15856) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/DNMT1-Antibody-8E3H1-Western-Blot-NBP3-15856-img0007.jpg "Western Blot: DNMT1 Antibody (8E3H1) [NBP3-15856] - Western blot analysis of extracts of various cell lines, using DNMT1 antibody (NBP3-15856) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Immunocytochemistry/Immunofluorescence: DNMT1 Antibody (8E3H1) [NBP3-15856] - Immunofluorescence analysis of C6 cells using DNMT1 antibody (NBP3-15856). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/DNMT1-Antibody-8E3H1-Immunocytochemistry-Immunofluorescence-NBP3-15856-img0006.jpg)
![Chromatin Immunoprecipitation: DNMT1 Antibody (8E3H1) [NBP3-15856] - Chromatin immunoprecipitation analysis of extracts of HeLa cells, using DNMT1 antibody (NBP3-15856) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/DNMT1-Antibody-8E3H1-Chromatin-Immunoprecipitation-NBP3-15856-img0009.jpg)
![Immunoprecipitation: DNMT1 Antibody (8E3H1) [NBP3-15856] - Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug DNMT1 antibody (NBP3-15856). Western blot was performed from the immunoprecipitate using DNMT1 (NBP3-15856) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/DNMT1-Antibody-8E3H1-Immunoprecipitation-NBP3-15856-img0008.jpg)
![Western Blot: DNMT1 Antibody (4G11-C7) [NBP2-59344] - Western Blot analysis of Human H1299 cell lysate showing detection of DNMT1 protein using Mouse Anti-DNMT1 Monoclonal Antibody, Clone 4G11-C7 (SMC-201). Primary Antibody: Mouse Anti-DNMT1 Monoclonal Antibody (SMC-201) at 1:1000.](http://images.novusbio.com/fullsize/DNMT1-Antibody-4G11-C7-Western-Blot-NBP2-59344-img0001.jpg "Western Blot: DNMT1 Antibody (4G11-C7) [NBP2-59344] - Western Blot analysis of Human H1299 cell lysate showing detection of DNMT1 protein using Mouse Anti-DNMT1 Monoclonal Antibody, Clone 4G11-C7 (SMC-201). Primary Antibody: Mouse Anti-DNMT1 Monoclonal Antibody (SMC-201) at 1:1000.")
![Immunohistochemistry-Paraffin: DNMT1 Antibody [NBP1-84328] - Staining in human placenta and pancreas tissues using anti-DNMT1 antibody. Corresponding DNMT1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/DNMT1-Antibody-Immunohistochemistry-Paraffin-NBP1-84328-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: DNMT1 Antibody [NBP1-84328] - Staining in human placenta and pancreas tissues using anti-DNMT1 antibody. Corresponding DNMT1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: DNMT1 Antibody [NBP1-84328] - Staining of human placenta shows high expression.](http://images.novusbio.com/fullsize/DNMT1-Antibody-Immunohistochemistry-Paraffin-NBP1-84328-img0006.jpg)
![Immunohistochemistry-Paraffin: DNMT1 Antibody [NBP1-84328] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/DNMT1-Antibody-Immunohistochemistry-Paraffin-NBP1-84328-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: DNMT1 Antibody [NBP2-98763] - Immunofluorescence staining of DNMT1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human DNMT1 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize/DNMT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98763-img0001.jpg "Immunocytochemistry/Immunofluorescence: DNMT1 Antibody [NBP2-98763] - Immunofluorescence staining of DNMT1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human DNMT1 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to nucleus.")
![Immunohistochemistry-Paraffin: DNMT1 Antibody [NBP2-98763] - Immunochemical staining of human DNMT1 in human placenta with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/DNMT1-Antibody-Immunohistochemistry-Paraffin-NBP2-98763-img0003.jpg)
![Immunohistochemistry-Paraffin: DNMT1 Antibody [NBP2-98763] - Immunochemical staining of human DNMT1 in human brain with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/DNMT1-Antibody-Immunohistochemistry-Paraffin-NBP2-98763-img0002.jpg)
![DNMT1 Assay/Inhibitor Screening Assay Kit [KA1515] - The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.](http://images.novusbio.com/fullsize/DNMT1-Assay-Inhibitor-Screening-Assay-Kit-KA1515-img0001.jpg "DNMT1 Assay/Inhibitor Screening Assay Kit [KA1515] - The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.")
![DNMT1 Assay/Inhibitor Screening Assay Kit [KA1515] - Positive control by using nuclear extract from MCF-7 cells.](http://images.novusbio.com/fullsize/DNMT1-Assay-Inhibitor-Screening-Assay-Kit-KA1515-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: DNMT1 Antibody (11H8) [NBP2-59694] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-DNMT1 Monoclonal Antibody, Clone 11H8 (NBP2-59694). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-DNMT1 Monoclonal Antibody (NBP2-59694) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) DNMT1 Antibody. (D) Composite.](http://images.novusbio.com/fullsize/DNMT1-Antibody-11H8-Immunocytochemistry-Immunofluorescence-NBP2-59694-img0003.jpg "Immunocytochemistry/Immunofluorescence: DNMT1 Antibody (11H8) [NBP2-59694] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-DNMT1 Monoclonal Antibody, Clone 11H8 (NBP2-59694). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-DNMT1 Monoclonal Antibody (NBP2-59694) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) DNMT1 Antibody. (D) Composite.")
![Immunohistochemistry: DNMT1 Antibody (1A9) [NBP3-26614] - Image of DNMT1 Antibody (1A9) diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26614_rabbit-dnmt1-mab-1a9-262202415475617.jpg "Immunohistochemistry: DNMT1 Antibody (1A9) [NBP3-26614] - Image of DNMT1 Antibody (1A9) diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunocytochemistry/Immunofluorescence: DNMT1 Antibody (1A9) [NBP3-26614] - Staining of HepG2 Cells with DNMT1 Antibody (1A9) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26614_rabbit-dnmt1-mab-1a9-282202412475138.jpg)
![Immunohistochemistry-Paraffin: DNMT1 Antibody (DNMT1/2061) - Azide and BSA Free [NBP3-08482] - Formalin-fixed, paraffin-embedded human Placenta stained with DNMT1 Mouse Monoclonal Antibody (DNMT1/2061).](http://images.novusbio.com/fullsize2/DNMT1-Antibody-(DNMT1-2061)---Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08482-img0001.jpg "Immunohistochemistry-Paraffin: DNMT1 Antibody (DNMT1/2061) - Azide and BSA Free [NBP3-08482] - Formalin-fixed, paraffin-embedded human Placenta stained with DNMT1 Mouse Monoclonal Antibody (DNMT1/2061).")
![Immunohistochemistry-Paraffin: DNMT1 Antibody (DNMT1/2061) - Azide and BSA Free [NBP3-08482] - Formalin-fixed, paraffin-embedded human Tonsil stained with DNMT1 Mouse Monoclonal Antibody (DNMT1/2061).](http://images.novusbio.com/fullsize2/DNMT1-Antibody-(DNMT1-2061)-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08482-img0002.jpg)