![DNMT1 Assay/Inhibitor Screening Assay Kit [KA1515] - The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.](http://images.novusbio.com/fullsize/DNMT1-Assay-Inhibitor-Screening-Assay-Kit-KA1515-img0001.jpg "DNMT1 Assay/Inhibitor Screening Assay Kit [KA1515] - The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.")
| Reactivity | MuSpecies Glossary |
| Applications | Func |
| Suitable Sample Type | Nuclear Extract, Purified Enzyme |
| Standard Curve Range | 0.5 to 20 ug nuclear extract, 0.5 to 200 ng purified enzyme |
| Sensitivity | 0.5 ug nuclear extract or 0.5 ng purified enzymes |
| Description | Quality control test: Standard Curve DNMT Activity/Inhibition Assay Ultra Kit (Colorimetric) is use for screening DNMT inhibitors. |
| Standard Curve Range | 0.5 to 20 ug nuclear extract, 0.5 to 200 ng purified enzyme |
| Sensitivity | 0.5 ug nuclear extract or 0.5 ng purified enzymes |
| Assay Type | Colorimetric |
| Sample Volume | 1-5 uL (5 to 10 ug nuclear extract or 10 to 100 ng purified enzyme included) |
| Kit Type | Activity/Inhibition Assay Kit (Colorimetric) |
| Gene | DNMT1 |
| Dilutions |
|
|
| Publications |
|
| Storage | Storage of components varies. See protocol for specific instructions. |
| Components |
|
![Immunocytochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunocytochemistry_15885.jpg)
![Immunohistochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunohistochemistry_15886.jpg)
![Immunocytochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunocytochemistry_16572.jpg)
Research Areas for DNMT1 Kit (KA1515)Find related products by research area.
|
|
The role of DNMT3B in the co-incidence of methyltransferase and tumor suppressor expression in malignancies Epigenetics is the process of heritable change in gene activity despite alteration of the hosts DNA sequence, essentially causing a change in a phenotype without a change in the genotype of a host. To change the gene sequence without interfering w... Read full blog post. |
|
The role of DNMT3A in development Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence. Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation. Gene silencing in DNA ... Read full blog post. |
|
Controlling Epigenetic Signaling with Dnmt1 and Dnmt3b Dnmt1 belongs to the C5-methyltransferase family that repairs cytosines in dsDNA using a nucleophilic attack mechanism. Dnmt1 is the most abundant mammalian DNA methyltransferase. It is the key methylation maintenance enzyme for both DNA replication/r... Read full blog post. |
|
PCNA Antibodies: Marking Cell Proliferation & DNA Replication Proliferating Cell Nuclear Antigen (PCNA), also known as the polymerase delta auxiliary protein, is a nuclear protein essential for DNA replication as well as DNA excision and mismatch repair pathways. It has a large role in cell cycle regulation and ... Read full blog post. |
|
DNMT's: An Overview of 3 DNA Methyltransferases DNA methyltransferases catalyze the transfer of the methyl group from S-andenosyl methionine (SAM) to DNA. Such methylation has wide ranging function in the cell, including organismal development and cell differentiation. In cancer, abnormal hypermeth... Read full blog post. |
| Gene Symbol | DNMT1 |
![DNMT1 Assay/Inhibitor Screening Assay Kit [KA1515] - Positive control by using nuclear extract from MCF-7 cells.](http://images.novusbio.com/fullsize/DNMT1-Assay-Inhibitor-Screening-Assay-Kit-KA1515-img0002.jpg "DNMT1 Assay/Inhibitor Screening Assay Kit [KA1515] - Positive control by using nuclear extract from MCF-7 cells.")
![Flow Cytometry: DNMT1 Antibody (60B1220.1) [NB100-56519] - Analysis of PE conjugate of NB100-56519. An intracellular stain was performed on HeLa cells with DNMT1 antibody (60B1220.1) NB100-56519PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with 0](https://images.novusbio.com/images/DNMT1-Antibody-60B1220-1-Flow-Cytometry-NB100-56519-img0009.jpg "Flow Cytometry: DNMT1 Antibody (60B1220.1) [NB100-56519] - Analysis of PE conjugate of NB100-56519. An intracellular stain was performed on HeLa cells with DNMT1 antibody (60B1220.1) NB100-56519PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with 0")