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![Western Blot: Calpastatin Antibody (1E7) [NBP2-01819] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Calpastatin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Calpastatin.](http://images.novusbio.com/fullsize/Calpastatin-Antibody-1E7-Western-Blot-NBP2-01819-img0002.jpg "Western Blot: Calpastatin Antibody (1E7) [NBP2-01819] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Calpastatin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Calpastatin.")
![Immunohistochemistry-Paraffin: Calpastatin Antibody (1E7) [NBP2-01819] Staining of paraffin-embedded Human prostate tissue using anti-Calpastatin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Calpastatin-Antibody-1E7-Immunohistochemistry-Paraffin-NBP2-01819-img0007.jpg)
![Flow Cytometry: Calpastatin Antibody (1E7) [NBP2-01819] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Calpastatin antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Calpastatin-Antibody-1E7-Flow-Cytometry-NBP2-01819-img0001.jpg)
![Immunohistochemistry-Paraffin: Calpastatin Antibody (1E7) [NBP2-01819] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Calpastatin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Calpastatin-Antibody-1E7-Immunohistochemistry-Paraffin-NBP2-01819-img0003.jpg)
![Immunohistochemistry-Paraffin: Calpastatin Antibody (1E7) [NBP2-01819] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-Calpastatin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Calpastatin-Antibody-1E7-Immunohistochemistry-Paraffin-NBP2-01819-img0004.jpg)
![Immunohistochemistry-Paraffin: Calpastatin Antibody (1E7) [NBP2-01819] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Calpastatin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Calpastatin-Antibody-1E7-Immunohistochemistry-Paraffin-NBP2-01819-img0005.jpg)
![Immunohistochemistry-Paraffin: Calpastatin Antibody (1E7) [NBP2-01819] - Staining of paraffin-embedded Human colon tissue using anti-Calpastatin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Calpastatin-Antibody-1E7-Immunohistochemistry-Paraffin-NBP2-01819-img0006.jpg)
![Immunohistochemistry-Paraffin: Calpastatin Antibody (CAST/1550) [NBP3-07397] - Formalin-fixed, paraffin-embedded human Tonsil stained with Calpastatin Mouse Monoclonal Antibody (CAST/1550).](http://images.novusbio.com/fullsize/Calpastatin-Antibody-CAST-1550-Immunohistochemistry-Paraffin-NBP3-07397-img0005.jpg "Immunohistochemistry-Paraffin: Calpastatin Antibody (CAST/1550) [NBP3-07397] - Formalin-fixed, paraffin-embedded human Tonsil stained with Calpastatin Mouse Monoclonal Antibody (CAST/1550).")
![Immunohistochemistry-Paraffin: Calpastatin Antibody (CAST/1550) [NBP3-07397] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Calpastatin Mouse Monoclonal Antibody (CAST/1550).](http://images.novusbio.com/fullsize/Calpastatin-Antibody-CAST-1550-Immunohistochemistry-Paraffin-NBP3-07397-img0001.jpg)
![Immunohistochemistry-Paraffin: Calpastatin Antibody (CAST/1550) [NBP3-07397] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Calpastatin Mouse Monoclonal Antibody (CAST/1550).](http://images.novusbio.com/fullsize/Calpastatin-Antibody-CAST-1550-Immunohistochemistry-Paraffin-NBP3-07397-img0002.jpg)
![Immunohistochemistry-Paraffin: Calpastatin Antibody (CAST/1550) [NBP3-07397] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Calpastatin Mouse Monoclonal Antibody (CAST/1550).](http://images.novusbio.com/fullsize/Calpastatin-Antibody-CAST-1550-Immunohistochemistry-Paraffin-NBP3-07397-img0003.jpg)
![Immunohistochemistry-Paraffin: Calpastatin Antibody (CAST/1550) [NBP3-07397] - Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with Calpastatin Mouse Monoclonal Antibody (CAST/1550).](http://images.novusbio.com/fullsize/Calpastatin-Antibody-CAST-1550-Immunohistochemistry-Paraffin-NBP3-07397-img0004.jpg)
![Western Blot: Calpastatin Antibody (2C5) [H00000831-M03] - Analysis of CAST expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/Calpastatin-Antibody-2C5-Western-Blot-H00000831-M03-img0005.jpg "Western Blot: Calpastatin Antibody (2C5) [H00000831-M03] - Analysis of CAST expression in NIH/3T3 (Cat # L018V1).")
![Immunocytochemistry/Immunofluorescence: Calpastatin Antibody (2C5) [H00000831-M03] - Analysis of monoclonal antibody to CAST on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/Calpastatin-Antibody-2C5-Immunocytochemistry-Immunofluorescence-H00000831-M03-img0004.jpg)
![Sandwich ELISA: Calpastatin Antibody (2C5) [H00000831-M03] - Detection limit for recombinant GST tagged CAST is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Calpastatin-Antibody-2C5-Sandwich-ELISA-H00000831-M03-img0003.jpg)
![Western Blot: Calpastatin Antibody [NBP2-15678] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE gel, diluted at 1:2000.](http://images.novusbio.com/fullsize/Calpastatin-Antibody-Western-Blot-NBP2-15678-img0001.jpg "Western Blot: Calpastatin Antibody [NBP2-15678] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE gel, diluted at 1:2000.")
![Immunohistochemistry-Paraffin: Calpastatin Antibody [NBP2-15678] - Paraffin-embedded human lung cancer. Calpastatin antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Calpastatin-Antibody-Immunohistochemistry-Paraffin-NBP2-15678-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Calpastatin Antibody [NBP2-38335] - Immunofluorescent staining of human cell line A549 shows localization to cytosol & endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Calpastatin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38335-img0003.jpg "Immunocytochemistry/Immunofluorescence: Calpastatin Antibody [NBP2-38335] - Immunofluorescent staining of human cell line A549 shows localization to cytosol & endoplasmic reticulum. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Calpastatin Antibody [NBP2-38335] - Staining of human skeletal muscle shows cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Calpastatin-Antibody-Immunohistochemistry-NBP2-38335-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Calpastatin Antibody [NBP2-38336] - Staining of human cell line A549 shows positivity in plasma membrane and cytoplasm.](http://images.novusbio.com/fullsize/Calpastatin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38336-img0001.jpg "Immunocytochemistry/Immunofluorescence: Calpastatin Antibody [NBP2-38336] - Staining of human cell line A549 shows positivity in plasma membrane and cytoplasm.")
![Immunohistochemistry: Calpastatin Antibody [NBP2-38336] - Staining of human prostate shows strong cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Calpastatin-Antibody-Immunohistochemistry-NBP2-38336-img0002.jpg)
![Western Blot: Calpastatin Antibody [NBP2-92941] - Analysis of extracts from normal (control) and CAST knockout (KO) HeLa cells, using Calpastatin antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non fat dry milk TBST. Detection: ECL Basic Kit . Exposure time: 5s](http://images.novusbio.com/fullsize/Calpastatin-Antibody-Western-Blot-NBP2-92941-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Calpastatin Antibody [NBP2-92941] - Analysis of extracts from normal (control) and CAST knockout (KO) HeLa cells, using Calpastatin antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non fat dry milk TBST. Detection: ECL Basic Kit . Exposure time: 5s")
![<b>Genetic Strategies Validation. </b>Western Blot: Calpastatin Antibody [NBP2-92941] - Analysis of extracts of various cell lines, using Calpastatin at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.](http://images.novusbio.com/fullsize/Calpastatin-Antibody-Western-Blot-NBP2-92941-img0002.jpg)
![SDS-Page: Recombinant Human Calpastatin Protein [H00000831-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-Calpastatin-Protein-SDS-Page-H00000831-P01-img0002.jpg "SDS-Page: Recombinant Human Calpastatin Protein [H00000831-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: Calpastatin Antibody [H00000831-B02P] - Analysis of CAST expression in transfected 293T cell line by CAST polyclonal antibody. Lane 1: CAST transfected lysate(75.46 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Calpastatin-Antibody-Western-Blot-H00000831-B02P-img0002.jpg "Western Blot: Calpastatin Antibody [H00000831-B02P] - Analysis of CAST expression in transfected 293T cell line by CAST polyclonal antibody. Lane 1: CAST transfected lysate(75.46 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Calpastatin Antibody [H00000831-B02P] - Analysis of purified antibody to CAST on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/Calpastatin-Antibody-Immunocytochemistry-Immunofluorescence-H00000831-B02P-img0001.jpg)
![ELISA: Mouse Calpastatin ELISA Kit (Colorimetric) [NBP2-75048] - Samples were spiked with high concentrations of Mouse Calpastatin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75048_mouse-calpastatin-elisa-kit-colorimetric-13220241601211.jpg "ELISA: Mouse Calpastatin ELISA Kit (Colorimetric) [NBP2-75048] - Samples were spiked with high concentrations of Mouse Calpastatin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Calpastatin ELISA Kit (Colorimetric) [NBP2-75048] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75048_mouse-calpastatin-elisa-kit-colorimetric-43202490353.jpg)
![SDS-Page: Recombinant Human Calpastatin Protein [H00000831-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-Calpastatin-Protein-SDS-Page-H00000831-Q01-img0001.jpg "SDS-Page: Recombinant Human Calpastatin Protein [H00000831-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
