Note: Mouseover a species abbreviation on the product page to display the fullname.
How can we help you?
![Simple Western: BAF53A Antibody [NB100-61628] - Simple Western lane view shows a specific band for BAF53A in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/BAF53A-Antibody-Simple-Western-NB100-61628-img0007.jpg "Simple Western: BAF53A Antibody [NB100-61628] - Simple Western lane view shows a specific band for BAF53A in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Western Blot: BAF53A Antibody [NB100-61628] - Western blot of WT and ARID1A-/- HCT116 nuclear extracts separated by glycerol gradient centrifugation. BAF53A antibody (NB100-61628) used for blotting. Representative of three biological replicates. Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/30506#content) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BAF53A-Antibody-Western-Blot-NB100-61628-img0038.jpg)
![Immunohistochemistry-Paraffin: BAF53A Antibody [NB100-61628] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BAF53A used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/BAF53A-Antibody-Immunohistochemistry-Paraffin-NB100-61628-img0035.jpg)
![Western Blot: BAF53A Antibody [NB100-61628] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-BAF53A antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/BAF53A-Antibody-Western-Blot-NB100-61628-img0036.jpg)
![Immunohistochemistry-Paraffin: BAF53A Antibody [NB100-61628] - Mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-BAF53A used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/BAF53A-Antibody-Immunohistochemistry-Paraffin-NB100-61628-img0034.jpg)
![Western Blot: BAF53A Antibody [NB100-1421] - Staining of NIH3T3 nuclear cell lysate (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/BAF53A-Antibody-Western-Blot-NB100-1421-img0002.jpg "Western Blot: BAF53A Antibody [NB100-1421] - Staining of NIH3T3 nuclear cell lysate (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: BAF53A Antibody [NB100-1421] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/BAF53A-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1421-img0004.jpg)
![Flow Cytometry: BAF53A Antibody [NB100-1421] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/BAF53A-Antibody-Flow-Cytometry-NB100-1421-img0005.jpg)
![Western Blot: BAF53A Antibody [NB100-1421] - Staining of Rat Skeletal Muscle lysate (35 ug protein in RIPA buffer). Antibody at 2 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/BAF53A-Antibody-Western-Blot-NB100-1421-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: BAF53A Antibody [NB100-1421] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/BAF53A-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1421-img0003.jpg)
![Western Blot: BAF53A Antibody (5U6J8) [NBP3-16659] - Western blot analysis of extracts of various cell lines, using BAF53A Rabbit mAb (NBP3-16659) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/BAF53A-Antibody-5U6J8-Western-Blot-NBP3-16659-img0005.jpg "Western Blot: BAF53A Antibody (5U6J8) [NBP3-16659] - Western blot analysis of extracts of various cell lines, using BAF53A Rabbit mAb (NBP3-16659) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: BAF53A Antibody (5U6J8) [NBP3-16659] - Immunohistochemistry of paraffin-embedded mouse kidney using BAF53A Rabbit mAb (NBP3-16659) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BAF53A-Antibody-5U6J8-Immunohistochemistry-Paraffin-NBP3-16659-img0004.jpg)
![Immunohistochemistry-Paraffin: BAF53A Antibody (5U6J8) [NBP3-16659] - Immunohistochemistry of paraffin-embedded rat brain using BAF53A Rabbit mAb (NBP3-16659) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BAF53A-Antibody-5U6J8-Immunohistochemistry-Paraffin-NBP3-16659-img0001.jpg)
![Immunohistochemistry-Paraffin: BAF53A Antibody (5U6J8) [NBP3-16659] - Immunohistochemistry of paraffin-embedded human appendix using BAF53A Rabbit mAb (NBP3-16659) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BAF53A-Antibody-5U6J8-Immunohistochemistry-Paraffin-NBP3-16659-img0002.jpg)
![Immunohistochemistry-Paraffin: BAF53A Antibody (5U6J8) [NBP3-16659] - Immunohistochemistry of paraffin-embedded human esophageal cancer using BAF53A Rabbit mAb (NBP3-16659) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BAF53A-Antibody-5U6J8-Immunohistochemistry-Paraffin-NBP3-16659-img0003.jpg)
![Western Blot: BAF53A Antibody [NBP2-55376] - Western blot analysis in human cell line RT-4, human cell line U-251 MG and human plasma.](http://images.novusbio.com/fullsize/BAF53A-Antibody-Western-Blot-NBP2-55376-img0002.jpg "Western Blot: BAF53A Antibody [NBP2-55376] - Western blot analysis in human cell line RT-4, human cell line U-251 MG and human plasma.")
![Immunocytochemistry/Immunofluorescence: BAF53A Antibody [NBP2-55376] - Staining of human cell line A-431 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/BAF53A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55376-img0001.jpg)
![Western Blot: BAF53A Antibody [NBP2-99367] - Anti-BAF53A rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 47kDa](http://images.novusbio.com/fullsize/BAF53A-Antibody-Western-Blot-NBP2-99367-img0003.jpg "Western Blot: BAF53A Antibody [NBP2-99367] - Anti-BAF53A rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 47kDa")
![Immunocytochemistry/Immunofluorescence: BAF53A Antibody [NBP2-99367] - Immunofluorescence staining of BAF53A in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human BAF53A polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to Nucleus .](http://images.novusbio.com/fullsize/BAF53A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-99367-img0001.jpg)
![Western Blot: BAF53A Antibody [NBP1-55378] - Transfected 293T cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/BAF53A-Antibody-Western-Blot-NBP1-55378-img0003.jpg "Western Blot: BAF53A Antibody [NBP1-55378] - Transfected 293T cell lysate, concentration 0.2-1 ug/ml.")
![Western Blot: BAF53A Antibody [NBP1-55378] - Mouse WT brain and Rat brain, concentration 2ug/ml.](http://images.novusbio.com/fullsize/BAF53A-Antibody-Western-Blot-NBP1-55378-img0002.jpg)
![SDS-Page: Recombinant Human BAF53A Protein [H00000086-P02] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-BAF53A-Protein-SDS-Page-H00000086-P02-img0001.jpg "SDS-Page: Recombinant Human BAF53A Protein [H00000086-P02] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![SDS-Page: Recombinant Human BAF53A Protein [H00000086-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-BAF53A-Protein-SDS-Page-H00000086-Q01-img0001.jpg "SDS-Page: Recombinant Human BAF53A Protein [H00000086-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![SDS-Page: Recombinant Human BAF53A Protein [H00000086-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-BAF53A-Protein-SDS-Page-H00000086-P01-img0001.jpg "SDS-Page: Recombinant Human BAF53A Protein [H00000086-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: BAF53A Antibody (3C4) [H00000086-M10] - Analysis of ACTL6A expression in transfected 293T cell line by ACTL6A monoclonal antibody (M10), clone 3C4. Lane 1: ACTL6A transfected lysatE (47.5 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/BAF53A-Antibody-3C4-Western-Blot-H00000086-M10-img0002.jpg "Western Blot: BAF53A Antibody (3C4) [H00000086-M10] - Analysis of ACTL6A expression in transfected 293T cell line by ACTL6A monoclonal antibody (M10), clone 3C4. Lane 1: ACTL6A transfected lysatE (47.5 KDa). Lane 2: Non-transfected lysate.")
![ELISA: BAF53A Antibody (3C4) [H00000086-M10] - Detection limit for recombinant GST tagged ACTL6A is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/BAF53A-Antibody-3C4-ELISA-H00000086-M10-img0001.jpg)
![Western Blot: BAF53A Antibody (S03-1G4) [NBP3-19983] - Western blot detection of BAF53A in Hela (LAne 1), MCF-7 (Lane 2), HepG2 (Lane 3), U2OS (Lane 4) using NBP3-19983 (1:1000 diluted)](http://images.novusbio.com/fullsize/BAF53A-Antibody-S03-1G4-Western-Blot-NBP3-19983-img0001.jpg "Western Blot: BAF53A Antibody (S03-1G4) [NBP3-19983] - Western blot detection of BAF53A in Hela (LAne 1), MCF-7 (Lane 2), HepG2 (Lane 3), U2OS (Lane 4) using NBP3-19983 (1:1000 diluted)")
![Western Blot: BAF53A Overexpression Lysate (Adult Normal) [NBL1-07276] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for BAF53A.](http://images.novusbio.com/fullsize/BAF53A-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07276-img0002.jpg "Western Blot: BAF53A Overexpression Lysate (Adult Normal) [NBL1-07276] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for BAF53A.")