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![Western Blot: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (OTI1B6) [NBP2-02483] - Analysis of extracts (10ug) from 2 different cell lines by using anti-ALDH3A1 monoclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-3-A1-ALDH3A1-Antibody-OTI1B6-Western-Blot-NBP2-02483-img0011.jpg "Western Blot: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (OTI1B6) [NBP2-02483] - Analysis of extracts (10ug) from 2 different cell lines by using anti-ALDH3A1 monoclonal antibody at 1:200 dilution.")
![Immunocytochemistry/Immunofluorescence: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (OTI1B6) [NBP2-02483] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ALDH3A1.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-3-A1-ALDH3A1-Antibody-1B6-Immunocytochemistry-Immunofluorescence-NBP2-02483-img0009.jpg)
![Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (OTI1B6) [NBP2-02483] - Staining of paraffin-embedded prostate tissue using anti-ALDH3A1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ALDH3A1-Antibody-1B6-Immunohistochemistry-Paraffin-NBP2-02483-img0007.jpg)
![Flow Cytometry: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (OTI1B6) [NBP2-02483] - HEK293T cells transfected with either pCMV6-ENTRY ALDH3A1.(Red) or empty vector control plasmid (Blue) were immunostained with anti-ALDH3A1 mouse monoclonal, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/ALDH3A1-Antibody-1B6-Flow-Cytometry-NBP2-02483-img0001.jpg)
![Western Blot: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (OTI1B6) [NBP2-02483] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALDH3A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALDH3A1.](http://images.novusbio.com/fullsize/ALDH3A1-Antibody-1B6-Western-Blot-NBP2-02483-img0002.jpg)
![Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (OTI1B6) [NBP2-02483] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-ALDH3A1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ALDH3A1-Antibody-1B6-Immunohistochemistry-Paraffin-NBP2-02483-img0003.jpg)
![Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (OTI1B6) [NBP2-02483] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-ALDH3A1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ALDH3A1-Antibody-1B6-Immunohistochemistry-Paraffin-NBP2-02483-img0004.jpg)
![Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (OTI1B6) [NBP2-02483] - Staining of paraffin-embedded liver tissue using anti-ALDH3A1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ALDH3A1-Antibody-1B6-Immunohistochemistry-Paraffin-NBP2-02483-img0005.jpg)
![Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (OTI1B6) [NBP2-02483] - Staining of paraffin-embedded pancreas tissue using anti-ALDH3A1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ALDH3A1-Antibody-1B6-Immunohistochemistry-Paraffin-NBP2-02483-img0006.jpg)
![Western Blot: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [NBP2-47551] - Analysis in human cell lines A-549 and U-251MG using anti-ALDH3A1 antibody. Corresponding ALDH3A1 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-3-A1-ALDH3A1-Antibody-Western-Blot-NBP2-47551-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [NBP2-47551] - Analysis in human cell lines A-549 and U-251MG using anti-ALDH3A1 antibody. Corresponding ALDH3A1 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [NBP2-47551] - Immunofluorescent staining of human cell line A549 shows localization to plasma membrane & cytosol.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-3-A1-ALDH3A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47551-img0004.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [NBP2-47551] - Staining in human esophagus and colon tissues using anti-ALDH3A1 antibody. Corresponding ALDH3A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-3-A1-ALDH3A1-Antibody-Immunohistochemistry-Paraffin-NBP2-47551-img0007.jpg)
![Western Blot: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [NBP2-47551] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-3-A1-ALDH3A1-Antibody-Western-Blot-NBP2-47551-img0003.jpg)
![Immunohistochemistry: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [NBP2-47551] - Staining of human stomach, lower shows strong cytoplasmic and nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-3-A1-ALDH3A1-Antibody-Immunohistochemistry-NBP2-47551-img0002.jpg)
![Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [NBP2-47551] - Staining of human colon shows low expression as expected.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-3-A1-ALDH3A1-Antibody-Immunohistochemistry-Paraffin-NBP2-47551-img0005.jpg)
![Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [NBP2-47551] - Staining of human esophagus shows high expression.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-3-A1-ALDH3A1-Antibody-Immunohistochemistry-Paraffin-NBP2-47551-img0006.jpg)
![Western Blot: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [NBP2-55797] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-3-A1-ALDH3A1-Antibody-Western-Blot-NBP2-55797-img0002.jpg "Western Blot: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [NBP2-55797] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [NBP2-55797] - Staining of human cell line A549 shows localization to nucleoplasm, cytosol & vesicles.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-3-A1-ALDH3A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55797-img0001.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [NBP2-55797] - Staining in human esophagus and colon tissues using anti-ALDH3A1 antibody. Corresponding ALDH3A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-3-A1-ALDH3A1-Antibody-Immunohistochemistry-Paraffin-NBP2-55797-img0005.jpg)
![Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [NBP2-55797] - Staining of human colon shows low expression as expected.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-3-A1-ALDH3A1-Antibody-Immunohistochemistry-Paraffin-NBP2-55797-img0003.jpg)
![Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [NBP2-55797] - Staining of human esophagus shows high expression.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-3-A1-ALDH3A1-Antibody-Immunohistochemistry-Paraffin-NBP2-55797-img0004.jpg)
![Western Blot: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [NBP2-15340] - Whole cell extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH3A1 antibody [N1C2] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-3-A1-ALDH3A1-Antibody-Western-Blot-NBP2-15340-img0002.jpg "Western Blot: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [NBP2-15340] - Whole cell extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH3A1 antibody [N1C2] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Frozen: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [NBP2-15340] - Analysis of frozen bovine cornea section, using ALDH3A1 antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-3-A1-ALDH3A1-Antibody-Immunohistochemistry-Frozen-NBP2-15340-img0001.jpg)
![Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [NBP2-15340] -Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH3A1 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/antibody/nbp2-15340_rabbit-polyclonal-aldehyde-dehydrogenase-3-a1-aldh3a1-antibody-western-blot-184202310279.jpg)
![Western Blot: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [NBP1-52052] - (0.01ug/ml) staining of Mouse Eye lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-3-A1-ALDH3A1-Antibody-Western-Blot-NBP1-52052-img0003.jpg "Western Blot: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [NBP1-52052] - (0.01ug/ml) staining of Mouse Eye lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [NBP1-52052] - Staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-3-A1-ALDH3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-52052-img0004.jpg)
![Western Blot: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Overexpression Lysate [NBP2-09567] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ALDH3A1.](http://images.novusbio.com/fullsize/ALDH3A1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09567-img0001.jpg "Western Blot: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Overexpression Lysate [NBP2-09567] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ALDH3A1.")
![Western Blot: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Overexpression Lysate [NBP2-09566] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ALDH3A1.](http://images.novusbio.com/fullsize/ALDH3A1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09566-img0001.jpg "Western Blot: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Overexpression Lysate [NBP2-09566] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ALDH3A1.")
![Western Blot: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Overexpression Lysate [NBL1-07455] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ALDH3A1.](http://images.novusbio.com/fullsize/ALDH3A1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07455-img0002.jpg "Western Blot: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Overexpression Lysate [NBL1-07455] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ALDH3A1.")
![Western Blot: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (316) [NBP2-90120] - Anti-ALDH3A1 rabbit monoclonal antibody at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:50 kDa Observed band size:50 kDa](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-3-A1-ALDH3A1-Antibody-316-Western-Blot-NBP2-90120-img0002.jpg "Western Blot: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (316) [NBP2-90120] - Anti-ALDH3A1 rabbit monoclonal antibody at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:50 kDa Observed band size:50 kDa")
![Immunoprecipitation: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (2G4) [H00000218-M08] - Analysis of ALDH3A1 transfected lysate using anti-ALDH3A1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ALDH3A1 rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-3-A1-ALDH3A1-Antibody-2G4-Immunoprecipitation-H00000218-M08-img0003.jpg "Immunoprecipitation: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (2G4) [H00000218-M08] - Analysis of ALDH3A1 transfected lysate using anti-ALDH3A1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ALDH3A1 rabbit polyclonal antibody.")
![ELISA: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (2G4) [H00000218-M08] - Detection limit for recombinant GST tagged ALDH3A1 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-3-A1-ALDH3A1-Antibody-2G4-ELISA-H00000218-M08-img0004.jpg)