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NBP2-02667

HID1 Antibody (OTI2F4)

(3 Publications)

0.1 ml

NBP2-02667

Western Blot: HID1 Antibody (OTI2F4) [NBP2-02667] - Analysis of extracts (10ug) from 10 Human tissue by using anti-C17orf28 monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

Immunocytochemistry/Immunofluorescence: HID1 Antibody (2F4) [NBP2-02667] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY C17orf28.

Immunohistochemistry-Paraffin: HID1 Antibody (2F4) [NBP2-02667] - Staining of paraffin-embedded Human prostate tissue using anti-C17orf28 mouse monoclonal antibody.

Flow Cytometry: HID1 Antibody (2F4) [NBP2-02667] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-C17orf28 antibody, and then analyzed by flow cytometry.

Western Blot: HID1 Antibody (2F4) [NBP2-02667] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C17orf28 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C17orf28.

Western Blot: HID1 Antibody (2F4) [NBP2-02667] - Analysis of extracts (35ug) from 9 different cell lines by using anti-C17orf28 monoclonal antibody.

Immunohistochemistry-Paraffin: HID1 Antibody (2F4) [NBP2-02667] - Staining of paraffin-embedded Human liver tissue using anti-C17orf28 mouse monoclonal antibody.

Flow Cytometry: HID1 Antibody (2F4) [NBP2-02667] - Analysis of Jurkat cells, using anti-C17orf28 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Compound MI-743 causes intracellular 8-oxo-dG accumulation and DNA damage.a MGC-803, HGC-27 and GES-1 cells were treated with DMSO or 5 µM MI-743 for 48 h. Intracellular 8-oxo-dG was stained with Cy3-conjugated avidin. DNA was counterstained with 4,6-diamidino-2-phenylindole (DAPI). Images were acquired at ×100 magnification by a Nikon Eclipse TE 2000-S fluorescence microscope. At least three independent experiments were performed for each group. b MGC-803, HGC-27 and GES-1 cells were treated with DMSO or 10 µM MI-743 for 48 h and run in alkaline comet assay. Pictures were originally captured at ×40 magnification. H2O2 was used as positive control. c Tail moment was calculated by CometScore software. Three individual experiments were performed for each group. d–f Western blot analysis of the protein levels of MTH1, MUTYH, OGG1, p21, ATMpS1981 and p53pS15 in MGC-803, HGC-27 and GES-1 cells, treated with increasing concentrations of MI-743 (0, 1, 2, 4, 8 and 12 µM). g, h Densitometry shows relative protein expression normalized for GAPDH. Data are representative of three independent experiments. i–k Western Blot analysis of the protein levels of MTH1, MUTYH, OGG1, p21, ATMpS1981 and p53pS15 of protein lysates, isolated from MGC-803, HGC-27 and GES-1 cells, which were treated with 12 µM MI-743 for 0, 12, 24, 36 and 48 h. l, m Densitometry shows relative protein expression normalized for GAPDH. Data are presented as means ± SD. Three individual experiments were performed for each group. *P < 0.05, **P < 0.01, ***P < 0.001 as compared with the controls Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31164636), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Ca, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Pm

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-72481AF350
Alexa Fluor 405	NBP2-72481AF405
Alexa Fluor 488	NBP2-72481AF488
Alexa Fluor 532	NBP2-72481AF532
Alexa Fluor 594	NBP2-72481AF594
Alexa Fluor 647	NBP2-72481AF647
Alexa Fluor 700	NBP2-72481AF700
Alexa Fluor 750	NBP2-72481AF750
Allophycocyanin	NBP2-72481APC
Allophycocyanin/Cy7	NBP2-72481APCCY7
Biotin	NBP2-72481B
DyLight 350	NBP2-72481UV
DyLight 405	NBP2-72481V
DyLight 488	NBP2-72481G
DyLight 550	NBP2-72481R
DyLight 594	NBP2-72481DL594
DyLight 650	NBP2-72481C
DyLight 680	NBP2-72481FR
DyLight 755	NBP2-72481IR
FITC	NBP2-72481F
HRP	NBP2-72481H
Janelia Fluor 549	NBP2-72481JF549
Janelia Fluor 646	NBP2-72481JF646
PE	NBP2-72481PE
PE/Atto594	NBP2-72481PEATT594
PE/Cy5.5	NBP2-72481PECY55
PE/Cy7	NBP2-72481PECY7
PerCP	NBP2-72481PCP
mFluor Violet 450 SE	NBP2-72481MFV450
mFluor Violet 500 SE	NBP2-72481MFV500
mFluor Violet 610 SE	NBP2-72481MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-72481

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NBP2-46123

CD1c/BDCA-1 Antibody (OTI2F4)

0.1 ml

NBP2-46123

Western Blot: CD1c/BDCA-1 Antibody (OTI2F4) [NBP2-46123] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD1c .

Immunocytochemistry/Immunofluorescence: CD1c/BDCA-1 Antibody (OTI2F4) [NBP2-46123] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY CD1C.

Immunohistochemistry: CD1c/BDCA-1 Antibody (OTI2F4) [NBP2-46123] - Analysis of Human endometrium tissue.

Western Blot: CD1c/BDCA-1 Antibody (OTI2F4) [NBP2-46123] - Analysis of Jurkat extracts (10ug).

Immunocytochemistry/Immunofluorescence: CD1c/BDCA-1 Antibody (OTI2F4) [NBP2-46123] - Analysis of HeLa cells.

Immunohistochemistry: CD1c/BDCA-1 Antibody (OTI2F4) [NBP2-46123] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: CD1c/BDCA-1 Antibody (OTI2F4) [NBP2-46123] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: CD1c/BDCA-1 Antibody (OTI2F4) [NBP2-46123] - Analysis of Carcinoma of Human lung tissue.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-70345AF350
Alexa Fluor 405	NBP2-70345AF405
Alexa Fluor 488	NBP2-70345AF488
Alexa Fluor 532	NBP2-70345AF532
Alexa Fluor 594	NBP2-70345AF594
Alexa Fluor 647	NBP2-70345AF647
Alexa Fluor 700	NBP2-70345AF700
Alexa Fluor 750	NBP2-70345AF750
Allophycocyanin	NBP2-70345APC
Biotin	NBP2-70345B
DyLight 350	NBP2-70345UV
DyLight 405	NBP2-70345V
DyLight 488	NBP2-70345G
DyLight 550	NBP2-70345R
DyLight 594	NBP2-70345DL594
DyLight 650	NBP2-70345C
DyLight 680	NBP2-70345FR
DyLight 755	NBP2-70345IR
FITC	NBP2-70345F
HRP	NBP2-70345H
Janelia Fluor 549	NBP2-70345JF549
Janelia Fluor 646	NBP2-70345JF646
PE	NBP2-70345PE
PerCP	NBP2-70345PCP

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-70345

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NBP2-45768

KIF25 Antibody (OTI2F4)

0.1 ml

NBP2-45768

Western Blot: KIF25 Antibody (OTI2F4) [NBP2-45768] - Analysis of extracts (35ug) from 9 different cell lines by usin g KIF25 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemistry: KIF25 Antibody (OTI2F4) [NBP2-45768] - Analysis of Human colon tissue.

Western Blot: KIF25 Antibody (OTI2F4) [NBP2-45768] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KIF25 .

Immunohistochemistry: KIF25 Antibody (OTI2F4) [NBP2-45768] - Analysis of Adenocarcinoma of Human colon tissue.

Immunofluorescence: KIF25 Antibody (OTI2F4) [NBP2-45768] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY KIF25.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse monoclonal

Host: Mouse monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-71077AF350
Alexa Fluor 405	NBP2-71077AF405
Alexa Fluor 488	NBP2-71077AF488
Alexa Fluor 532	NBP2-71077AF532
Alexa Fluor 594	NBP2-71077AF594
Alexa Fluor 647	NBP2-71077AF647
Alexa Fluor 700	NBP2-71077AF700
Alexa Fluor 750	NBP2-71077AF750
Allophycocyanin	NBP2-71077APC
Biotin	NBP2-71077B
DyLight 350	NBP2-71077UV
DyLight 405	NBP2-71077V
DyLight 488	NBP2-71077G
DyLight 550	NBP2-71077R
DyLight 594	NBP2-71077DL594
DyLight 650	NBP2-71077C
DyLight 680	NBP2-71077FR
DyLight 755	NBP2-71077IR
FITC	NBP2-71077F
HRP	NBP2-71077H
Janelia Fluor 549	NBP2-71077JF549
Janelia Fluor 646	NBP2-71077JF646
PE	NBP2-71077PE
PerCP	NBP2-71077PCP

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-71077

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NBP2-45414

IL6R Antibody (OTI2F4)

(1 Publication)

0.1 ml

NBP2-45414

Western Blot: IL6R Antibody (OTI2F4) [NBP2-45414] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL6R.

Immunohistochemistry-Paraffin: IL6R Antibody (OTI2F4) [NBP2-45414] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunofluorescence: IL6R Antibody (OTI2F4) [NBP2-45414] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY IL6R.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-71029AF350
Alexa Fluor 405	NBP2-71029AF405
Alexa Fluor 488	NBP2-71029AF488
Alexa Fluor 532	NBP2-71029AF532
Alexa Fluor 594	NBP2-71029AF594
Alexa Fluor 647	NBP2-71029AF647
Alexa Fluor 700	NBP2-71029AF700
Alexa Fluor 750	NBP2-71029AF750
Allophycocyanin	NBP2-71029APC
Biotin	NBP2-71029B
DyLight 350	NBP2-71029UV
DyLight 405	NBP2-71029V
DyLight 488	NBP2-71029G
DyLight 550	NBP2-71029R
DyLight 594	NBP2-71029DL594
DyLight 650	NBP2-71029C
DyLight 680	NBP2-71029FR
DyLight 755	NBP2-71029IR
FITC	NBP2-71029F
HRP	NBP2-71029H
Janelia Fluor 549	NBP2-71029JF549
Janelia Fluor 646	NBP2-71029JF646
PE	NBP2-71029PE
PerCP	NBP2-71029PCP

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-71029

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NBP2-45749

IL-36 gamma/IL-1F9 Antibody (OTI2F4)

0.1 ml

NBP2-45749

Western Blot: IL-36 gamma/IL-1F9 Antibody (OTI2F4) [NBP2-45749] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL1F9.

Immunocytochemistry/Immunofluorescence: IL-36 gamma/IL-1F9 Antibody (OTI2F4) [NBP2-45749] - Analysis of HeLa cells.

Immunohistochemistry: IL-36 gamma/IL-1F9 Antibody (OTI2F4) [NBP2-45749] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Western Blot: IL-36 gamma/IL-1F9 Antibody (OTI2F4) [NBP2-45749] - Analysis of extracts (10ug).

Immunohistochemistry: IL-36 gamma/IL-1F9 Antibody (OTI2F4) [NBP2-45749] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-72055AF350
Alexa Fluor 405	NBP2-72055AF405
Alexa Fluor 488	NBP2-72055AF488
Alexa Fluor 532	NBP2-72055AF532
Alexa Fluor 594	NBP2-72055AF594
Alexa Fluor 647	NBP2-72055AF647
Alexa Fluor 700	NBP2-72055AF700
Alexa Fluor 750	NBP2-72055AF750
Allophycocyanin	NBP2-72055APC
Biotin	NBP2-72055B
DyLight 350	NBP2-72055UV
DyLight 405	NBP2-72055V
DyLight 488	NBP2-72055G
DyLight 550	NBP2-72055R
DyLight 594	NBP2-72055DL594
DyLight 650	NBP2-72055C
DyLight 680	NBP2-72055FR
DyLight 755	NBP2-72055IR
FITC	NBP2-72055F
HRP	NBP2-72055H
Janelia Fluor 549	NBP2-72055JF549
Janelia Fluor 646	NBP2-72055JF646
PE	NBP2-72055PE
PerCP	NBP2-72055PCP

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-72055

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