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7 Results for "novus"

NBP1-47491

VCAM-1/CD106 Antibody (6G9) - BSA Free

(2 Reviews)

(5 Publications)

0.1 ml

NBP1-47491

Western Blot: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - Using VCAM1 mouse mAb against (1) HUVEC and (2) EC cell lysate.

Immunohistochemistry: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - Photomicrographs of two separate gut sections from a patient with EHEC colitis. Panels (C) and (D) are stained to show VCAM-1/CD106 expression in endothelium, indicating inflammatory activation (40x magnification). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0055278), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - (A) human lung cancer and (B) colon cancer using VCAM1 mouse mAb with DAB staining.

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - Human placenta tissues using VCAM1 mouse mAb.

Immunohistochemistry: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - Breast carcinoma, cytoplasmic staining. IHC image submitted by a verified customer review.

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - FFPE section of human lung cancer. Image at 20X magnification. IHC-P image submitted by a verified customer review.

Simple Western: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - Lane view shows a specific band for CD106/VCAM1 in 0.5 mg/mL of HUVEC lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Photomicrographs of two separate gut sections from a patient with EHEC colitis.Panels (A) and (B) are stained with CD31 to enumerate endothelium lining the vessels (40× magnification). (C) and (D) are stained to show VCAM-1 expression in endothelium, indicating inflammatory activation (40× magnification).

Small interference RNA targeting Raptor and Rictor disrupts BMAL1 accumulation in HNSCCTargeted disruption of Raptor (A-B) and Rictor (C-D) using siRNA results in a dose-dependent downregulation of BMAL1 in HNSCC cells. E. Disruption of PTEN by protein oxidation causes activation of mTOR signaling, resulting in accumulation of BMAL1. Notably, inhibition of mTOR signaling, particularly mTORC1 and mTORC2, results in restoration of normal BMAL1 levels in the epidermis of mice and head and neck cancer cells. These results demonstrate a novel role for mTOR in regulating nuclear levels of the core clock gene BMAL1. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/27285754), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Identification of an HDR-enhancing fragment of 53BP1. a Schematic diagram of full-length human 53BP1 protein showing the minimal focus forming region including the oligomerization domain (OD), a glycine-arginine rich (GAR) motif, a tandem Tudor domain, and the ubiquitin-dependent recruitment (UDR) motif. BRCT repeats at the extreme C-terminus are present. b Different truncated 53BP1 proteins, DN1, DN1S, DN2, DN3, and DN4, were tested for their relative expression by western blot using anti-HA antibody in HeLa cells transduced with empty vector (mock) or lentiviral vectors encoding HA-tagged 53BP1 fragments DN1, DN1S, DN2, DN3, or DN4. Actin is shown as a loading control. c, e, g Representative immunofluorescence (IF) images of HA-tagged DN1 and DN1S at nuclear foci. Cells were either exposed to 2 Gy IR or no IR (control) and fixed 2 h later. Scale bars represent 20 μm. c Representative IF images showing co-localization of HA-tagged DN1 or DN1S with endogenous 53BP1 foci. d Number (no.) of HA+ (DN1S) foci, co-localized HA+/endogenous 53BP1+ (co-localization) foci, or endogenous 53BP1+ (E-53BP1) only foci per IR-treated HeLa cell expressing DN1S. Individual quantifications of foci from 50 cells in each group are shown. Red lines are drawn at the mean number of foci per positive cell. e Representative IF images showing HA-tagged DN1 or DN1S and RIF1 recruitment to IR-induced DNA repair foci. f Quantification of cells with ≥3 RIF1 foci in control cells or IR cells with DN1, DN1S or without vector (mock). g Representative IF images showing HA-tagged DN1S and BRCA1 recruitment to DNA repair foci in control cells. h Quantification of the number of cells with ≥3 BRCA1 foci in control cells or IR cells, with DN1, DN1S or without vector (mock). In panels f and g, data are presented as the mean ± SEM of counts of 150 cells each, from three independent fields, and black circles indicate individual counts. Statistics for panels d, f, and g: ANOVA. ns indicates not significant, *p < 0.05, ***p < 0.001, and ****p < 0.0001. Source data is available in Source Data file Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31253785), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-47491AF350
Alexa Fluor 405	NBP1-47491AF405
Alexa Fluor 488	NBP1-47491AF488
Alexa Fluor 532	NBP1-47491AF532
Alexa Fluor 594	NBP1-47491AF594
Alexa Fluor 647	NBP1-47491AF647
Alexa Fluor 700	NBP1-47491AF700
Alexa Fluor 750	NBP1-47491AF750
Biotin	NBP1-47491B
DyLight 350	NBP1-47491UV
DyLight 405	NBP1-47491V
DyLight 488	NBP1-47491G
DyLight 550	NBP1-47491R
DyLight 594	NBP1-47491DL594
DyLight 650	NBP1-47491C
DyLight 680	NBP1-47491FR
DyLight 755	NBP1-47491IR
FITC	NBP1-47491F
HRP	NBP1-47491H
Janelia Fluor 549	NBP1-47491JF549
Janelia Fluor 646	NBP1-47491JF646
mFluor Violet 450 SE	NBP1-47491MFV450
mFluor Violet 500 SE	NBP1-47491MFV500
mFluor Violet 610 SE	NBP1-47491MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-47491SS
Azide and BSA Free	NBP2-81005

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NB100-1983

CaM Kinase II alpha Antibody (6G9)

(14 Publications)

0.1 mg

NB100-1983

Western Blot: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Western Blot analysis of Rat brain membrane lysate showing detection of CaM Kinase II alpha protein using Mouse Anti-CaM Kinase II alpha Monoclonal Antibody, Clone 6G9 (NB100-1983). Primary Antibody: Mouse Anti-CaM Kinase II alpha Monoclonal Antibody (NB100-1983) at 1:1000.

Immunocytochemistry/Immunofluorescence: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CaM Kinase II alpha Monoclonal Antibody, Clone 6G9 (NB100-1983). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-CaM Kinase II alpha Monoclonal Antibody (NB100-1983) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Nuclear Staining.

Immunohistochemistry: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Immunohistochemistry analysis using Mouse Anti-CaM Kinase II alpha Monoclonal Antibody, Clone 6G9 (NB100-1983). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-CaM Kinase II alpha Monoclonal Antibody (NB100-1983) at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Magnification: 40x.

Immunocytochemistry/Immunofluorescence: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Hair follicles, epidermis.

Immunocytochemistry/Immunofluorescence: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Tissue: dissociated hippocampal neurons. Species: Mouse. Fixation: Cold 4% paraformaldehyde/0.2% glutaraldehyde in 0.1M sodium phosphate buffer. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:1000 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse IgG (green) at 1:50 for 30 minutes at RT. Magnification: 10X. Courtesy of: Mary Kennedy, Caltech.

Immunohistochemistry: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Immunohistochemistry analysis using Mouse Anti-CaM Kinase II alpha Monoclonal Antibody, Clone 6G9 (NB100-1983). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaM Kinase II alpha Monoclonal Antibody (NB100-1983) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Hair follicles, epidermis.

Reactivity:

Hu, Mu, Rt, Bv, Xp

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Xp

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	NB100-1983B
DyLight 550	NB100-1983R
DyLight 650	NB100-1983C
FITC	NB100-1983F
HRP	NB100-1983H

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H00010926-M01

DBF4 Antibody (6G9)

(1 Publication)

0.1 mg

H00010926-M01

Western Blot: DBF4 Antibody (6G9) [H00010926-M01] - Analysis of DBF4 expression in transfected 293T cell line by DBF4 monoclonal antibody (M01), clone 6G9.Lane 1: DBF4 transfected lysate(76.9 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: DBF4 Antibody (6G9) [H00010926-M01] - Analysis of monoclonal antibody to DBF4 on HeLa cell . Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: DBF4 Antibody (6G9) [H00010926-M01] - Immunoperoxidase of monoclonal antibody to DBF4 ( Cat # H00010926-M01 ) on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3 ug/ml]

Sandwich ELISA: DBF4 Antibody (6G9) [H00010926-M01] - Detection limit for recombinant GST tagged DBF4 is approximately 0.1ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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H00008518-M03

IKBKAP Antibody (6G9)

(2 Publications)

0.1 mg

H00008518-M03

Western Blot: IKBKAP Antibody (6G9) [H00008518-M03] - Analysis of IKBKAP expression in HeLa (Cat # L013V1).

Immunohistochemistry-Paraffin: IKBKAP Antibody (6G9) [H00008518-M03] - Analysis of monoclonal antibody to IKBKAP on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml

ELISA: IKBKAP Antibody (6G9) [H00008518-M03] - Detection limit for recombinant GST tagged IKBKAP is approximately 0.3ng/ml as a capture antibody.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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H00001073-M03

Cofilin 2 Antibody (6G9)

(1 Publication)

0.1 mg

H00001073-M03

Western Blot: Cofilin 2 Antibody (6G9) [H00001073-M03] - Analysis of CFL2 expression in HeLa (Cat # L013V1).

Immunocytochemistry/Immunofluorescence: Cofilin 2 Antibody (6G9) [H00001073-M03] - Analysis of monoclonal antibody to CFL2 on HeLa cell. Antibody concentration 10 ug/ml

Immunohistochemistry-Paraffin: Cofilin 2 Antibody (6G9) [H00001073-M03] - Analysis of monoclonal antibody to CFL2 on formalin-fixed paraffin-embedded human skeletal muscle. Antibody concentration 8 ug/ml

ELISA: Cofilin 2 Antibody (6G9) [H00001073-M03] - Detection limit for recombinant GST tagged CFL2 is approximately 1ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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H00006732-M03

SRPK1 Antibody (6G9)

0.1 mg

H00006732-M03

Western Blot: SRPK1 Antibody (6G9) [H00006732-M03] - Analysis of SRPK1 expression in Jurkat (Cat # L017V1).

Western Blot: SRPK1 Antibody (6G9) [H00006732-M03] - Analysis of SRPK1 expression in HepG2 (Cat # L019V1).

Western Blot: SRPK1 Antibody (6G9) [H00006732-M03] - Analysis of SRPK1 expression in transfected 293T cell line by SRPK1 monoclonal antibody (M03), clone 6G9. Lane 1: SRPK1 transfected lysatE (92.407 KDa). Lane 2: Non-transfected lysate.

Sandwich ELISA: SRPK1 Antibody (6G9) [H00006732-M03] - Detection limit for recombinant GST tagged SRPK1 is approximately 0.1ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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H00026263-M01

FBXO22 Antibody (6G9)

0.1 mg

H00026263-M01

Western Blot: FBXO22 Antibody (6G9) [H00026263-M01] - FBXO22 monoclonal antibody (M01), clone 6G9 Analysis of FBXO22 expression in Hela S3 NE.

Sandwich ELISA: FBXO22 Antibody (6G9) [H00026263-M01] - Detection limit for recombinant GST tagged FBXO22 is approximately 0.3ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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