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![Knockdown Validated: hnRNP A1 Antibody (4B10) [NB100-672] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and hnRNP A1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human hnRNP A1 Monoclonal Antibody (Catalog # NB100-672) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for hnRNP A1 at approximately 35 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/hnRNP-A1-Antibody-4B10-Knockdown-Validated-NB100-672-img0004.jpg "<b>Genetic Strategies Validation. </b>Knockdown Validated: hnRNP A1 Antibody (4B10) [NB100-672] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and hnRNP A1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human hnRNP A1 Monoclonal Antibody (Catalog # NB100-672) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for hnRNP A1 at approximately 35 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: hnRNP A1 Antibody (4B10) [NB100-672] - Whole cell protein from HeLa (lane 1), A431 (lane 2), Hek293 (lane 3) and MCF7 (lane 4) were separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 mg/ml anti-hnRNP in 1% milk. Precision Plus Protein All Blue molecular weight markers (BioRad) were detected with 1 ug/ml Anti-Blue Marker antibody (NBP2-33376). Both antibodies were detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/hnRNP-A1-Antibody-4B10-Western-Blot-NB100-672-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: hnRNP A1 Antibody (4B10) [NB100-672] - The hnRNP A1 (4B10) antibody was tested in HeLa cells at a 1:200 dilution against Dylight 488 (Green). Actin and nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/hnRNP-A1-Antibody-4B10-Immunocytochemistry-Immunofluorescence-NB100-672-img0002.jpg)
![Western Blot: hnRNP A1 Antibody (4B10) [NB100-672] - Cell line lysates were separated on SDS-PAGE and probed with 0.5 ug/mL Monoclonal Anti-hnRNP-A1 Clone: 4B10 (NB100-672). Lanes: 1-HeLa, 2-Jurkat, 3-HEK-293T, 4-WiDr, 5-NCI-H1299, 6-F9.](http://images.novusbio.com/fullsize/hnRNP-A1-Antibody-4B10-Western-Blot-NB100-672-img0001.jpg)
![Western Blot: HIP1 Antibody (4B10) [NB300-203] - Western Blot on HeLa whole cell extracts.](http://images.novusbio.com/fullsize/HIP1-Antibody-4B10-Western-Blot-NB300-203-img0003.jpg "Western Blot: HIP1 Antibody (4B10) [NB300-203] - Western Blot on HeLa whole cell extracts.")
![Western Blot: Lamin B1 Antibody (4B10) [H00004001-M01] - Analysis of LMNB1 expression in transfected 293T cell line by LMNB1 monoclonal antibody (M01), clone 4B10.Lane 1: LMNB1 transfected lysate(66.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-4B10-Western-Blot-H00004001-M01-img0009.jpg "Western Blot: Lamin B1 Antibody (4B10) [H00004001-M01] - Analysis of LMNB1 expression in transfected 293T cell line by LMNB1 monoclonal antibody (M01), clone 4B10.Lane 1: LMNB1 transfected lysate(66.4 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Lamin B1 Antibody (4B10) [H00004001-M01] - Analysis of monoclonal antibody to LMNB1 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-4B10-Immunocytochemistry-Immunofluorescence-H00004001-M01-img0003.jpg)
![Western Blot: Lamin B1 Antibody (4B10) [H00004001-M01] - LMNB1 monoclonal antibody (M01), clone 4B10. Analysis of LMNB1 expression in Raw 264.7.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-4B10-Western-Blot-H00004001-M01-img0006.jpg)
![Western Blot: Lamin B1 Antibody (4B10) [H00004001-M01] - LMNB1 monoclonal antibody (M01), clone 4B10. Analysis of LMNB1 expression in Jurkat.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-4B10-Western-Blot-H00004001-M01-img0007.jpg)
![Western Blot: Lamin B1 Antibody (4B10) [H00004001-M01] - LMNB1 monoclonal antibody (M01), clone 4B10. Analysis of LMNB1 expression in NIH/3T3.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-4B10-Western-Blot-H00004001-M01-img0008.jpg)
![Immunoprecipitation: Lamin B1 Antibody (4B10) [H00004001-M01] - Analysis of LMNB1 transfected lysate using anti-LMNB1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LMNB1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-4B10-Immunoprecipitation-H00004001-M01-img0004.jpg)
![ELISA: Lamin B1 Antibody (4B10) [H00004001-M01] - Detection limit for recombinant GST tagged LMNB1 is 3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-4B10-ELISA-H00004001-M01-img0010.jpg)
![Western Blot: LMW-PTP/ACP1 Antibody (4B10) [H00000052-M01] - Analysis of ACP1 expression in transfected 293T cell line by ACP1 monoclonal antibody (M01), clone 4B10.Lane 1: ACP1 transfected lysate(18 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/LMW-PTP-ACP1-Antibody-4B10-Western-Blot-H00000052-M01-img0018.jpg "Western Blot: LMW-PTP/ACP1 Antibody (4B10) [H00000052-M01] - Analysis of ACP1 expression in transfected 293T cell line by ACP1 monoclonal antibody (M01), clone 4B10.Lane 1: ACP1 transfected lysate(18 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: LMW-PTP/ACP1 Antibody (4B10) [H00000052-M01] - ACP1 monoclonal antibody (M01), clone 4B10. Analysis of ACP1 expression in HeLa.](http://images.novusbio.com/fullsize/LMW-PTP-ACP1-Antibody-4B10-Western-Blot-H00000052-M01-img0014.jpg)
![Western Blot: LMW-PTP/ACP1 Antibody (4B10) [H00000052-M01] - ACP1 monoclonal antibody (M01), clone 4B10. Analysis of ACP1 expression in PC-12.](http://images.novusbio.com/fullsize/LMW-PTP-ACP1-Antibody-4B10-Western-Blot-H00000052-M01-img0015.jpg)
![Western Blot: LMW-PTP/ACP1 Antibody (4B10) [H00000052-M01] - ACP1 monoclonal antibody (M01), clone 4B10. Analysis of ACP1 expression in Raw 264.7.](http://images.novusbio.com/fullsize/LMW-PTP-ACP1-Antibody-4B10-Western-Blot-H00000052-M01-img0016.jpg)
![Western Blot: LMW-PTP/ACP1 Antibody (4B10) [H00000052-M01] - ACP1 monoclonal antibody (M01), clone 4B10. Analysis of ACP1 expression in NIH/3T3.](http://images.novusbio.com/fullsize/LMW-PTP-ACP1-Antibody-4B10-Western-Blot-H00000052-M01-img0017.jpg)
![ELISA: LMW-PTP/ACP1 Antibody (4B10) [H00000052-M01] - Detection limit for recombinant GST tagged ACP1 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/LMW-PTP-ACP1-Antibody-4B10-ELISA-H00000052-M01-img0013.jpg)
![Western Blot: CD3 zeta Antibody (4B10) [NBP1-42561] - Analysis using CD247 mAb against CD247(AA: 52-164)-hIgGFc transfected HEK293 cell lysate.](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-4B10-Western-Blot-NBP1-42561-img0011.jpg "Western Blot: CD3 zeta Antibody (4B10) [NBP1-42561] - Analysis using CD247 mAb against CD247(AA: 52-164)-hIgGFc transfected HEK293 cell lysate.")
![Immunocytochemistry/Immunofluorescence: CD3 zeta Antibody (4B10) [NBP1-42561] - Analysis of K562 cells using anti-CD247 mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-4B10-Immunocytochemistry-Immunofluorescence-NBP1-42561-img0009.jpg)
![Immunohistochemistry-Paraffin: CD3 zeta Antibody (4B10) [NBP1-42561] - Analysis of human Thymus tissues using anti-CD247 mouse mAb](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-4B10-Immunohistochemistry-Paraffin-NBP1-42561-img0010.jpg)
![Flow Cytometry: CD3 zeta Antibody (4B10) [NBP1-42561] - Analysis of Jurkat cells using anti-CD247 mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-4B10-Flow-Cytometry-NBP1-42561-img0012.jpg)
![Western Blot: BAP29 Antibody (4B10) [H00055973-M07] - Analysis of BCAP29 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/BAP29-Antibody-4B10-Western-Blot-H00055973-M07-img0007.jpg "Western Blot: BAP29 Antibody (4B10) [H00055973-M07] - Analysis of BCAP29 expression in PC-12 (Cat # L012V1).")
![Immunocytochemistry/Immunofluorescence: BAP29 Antibody (4B10) [H00055973-M07] - Analysis of monoclonal antibody to BCAP29 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/BAP29-Antibody-4B10-Immunocytochemistry-Immunofluorescence-H00055973-M07-img0005.jpg)
![Immunohistochemistry-Paraffin: BAP29 Antibody (4B10) [H00055973-M07] - Analysis of monoclonal antibody to BCAP29 on formalin-fixed paraffin-embedded human colon. Antibody concentration 1.5 ug/ml](http://images.novusbio.com/fullsize/BAP29-Antibody-4B10-Immunohistochemistry-Paraffin-H00055973-M07-img0006.jpg)
![ELISA: BAP29 Antibody (4B10) [H00055973-M07] - Detection limit for recombinant GST tagged BCAP29 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/BAP29-Antibody-4B10-ELISA-H00055973-M07-img0004.jpg)
![Western Blot: E4F1 Antibody (4B10) [H00001877-M03] - Analysis of E4F1 expression in transfected 293T cell line by E4F1 monoclonal antibody (M03), clone 4B10. Lane 1: E4F1 transfected lysatE (83.5 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/E4F1-Antibody-4B10-Western-Blot-H00001877-M03-img0004.jpg "Western Blot: E4F1 Antibody (4B10) [H00001877-M03] - Analysis of E4F1 expression in transfected 293T cell line by E4F1 monoclonal antibody (M03), clone 4B10. Lane 1: E4F1 transfected lysatE (83.5 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: E4F1 Antibody (4B10) [H00001877-M03] - Analysis of monoclonal antibody to E4F1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/E4F1-Antibody-4B10-Immunocytochemistry-Immunofluorescence-H00001877-M03-img0002.jpg)
![Sandwich ELISA: E4F1 Antibody (4B10) [H00001877-M03] - Detection limit for recombinant GST tagged E4F1 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/E4F1-Antibody-4B10-Sandwich-ELISA-H00001877-M03-img0003.jpg)
![Western Blot: PTGIR Antibody (4B10) [H00005739-M01] - Analysis of PTGIR expression in transfected 293T cell line by PTGIR monoclonal antibody (M01), clone 4B10.Lane 1: PTGIR transfected lysate(41 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PTGIR-Antibody-4B10-Western-Blot-H00005739-M01-img0009.jpg "Western Blot: PTGIR Antibody (4B10) [H00005739-M01] - Analysis of PTGIR expression in transfected 293T cell line by PTGIR monoclonal antibody (M01), clone 4B10.Lane 1: PTGIR transfected lysate(41 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: PTGIR Antibody (4B10) [H00005739-M01] - Analysis of monoclonal antibody to PTGIR on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/PTGIR-Antibody-4B10-Immunohistochemistry-Paraffin-H00005739-M01-img0006.jpg)
![Western Blot: PTGIR Antibody (4B10) [H00005739-M01] - PTGIR monoclonal antibody (M01), clone 4B10 Analysis of PTGIR expression in A-549.](http://images.novusbio.com/fullsize/PTGIR-Antibody-4B10-Western-Blot-H00005739-M01-img0008.jpg)
![ELISA: PTGIR Antibody (4B10) [H00005739-M01] - Detection limit for recombinant GST tagged PTGIR is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PTGIR-Antibody-4B10-ELISA-H00005739-M01-img0007.jpg)
![Western Blot: T-bet/TBX21 Antibody (4B10) [NBP1-43298] - CD4+ T cells were sorted from mouse spleen, activated with Anti-Mouse CD3 and Anti-Mouse CD28, followed by culture in Th1-polarizing conditions, and re-activation with PMA and Ionomycin. Lysates from control (left) or PMA and Ionomycin-re-activated (right](http://images.novusbio.com/fullsize/T-bet-TBX21-Antibody-4B10-Western-Blot-NBP1-43298-img0002.jpg "Western Blot: T-bet/TBX21 Antibody (4B10) [NBP1-43298] - CD4+ T cells were sorted from mouse spleen, activated with Anti-Mouse CD3 and Anti-Mouse CD28, followed by culture in Th1-polarizing conditions, and re-activation with PMA and Ionomycin. Lysates from control (left) or PMA and Ionomycin-re-activated (right")
![Flow (Intracellular): T-bet/TBX21 Antibody (4B10) [NBP1-43298] - Intracellular staining of normal human peripheral blood cells with Anti-Human CD8a APC and 0.125 ug of Anti-Human/Mouse T-bet PerCP-Cy5.5 using the Foxp3 Fixation/Permeabilization Buffers . Cells in the lymphocyte gate were used for analysis. Image using the PerCP/Cy5.5 format of this antibody.](http://images.novusbio.com/fullsize/T-bet-TBX21-Antibody-4B10-Flow-Intracellular-NBP1-43298-img0005.jpg)
![Western Blot: AAK1 Antibody (4B10) [H00022848-M02] - Analysis of AAK1 expression in transfected 293T cell line by AAK1 monoclonal antibody (M02), clone 4B10.Lane 1: AAK1 transfected lysate(52 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/AAK1-Antibody-4B10-Western-Blot-H00022848-M02-img0005.jpg "Western Blot: AAK1 Antibody (4B10) [H00022848-M02] - Analysis of AAK1 expression in transfected 293T cell line by AAK1 monoclonal antibody (M02), clone 4B10.Lane 1: AAK1 transfected lysate(52 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: AAK1 Antibody (4B10) [H00022848-M02] - Analysis of monoclonal antibody to AAK1 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/AAK1-Antibody-4B10-Immunocytochemistry-Immunofluorescence-H00022848-M02-img0003.jpg)
![Sandwich ELISA: AAK1 Antibody (4B10) [H00022848-M02] - Detection limit for recombinant GST tagged AAK1 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/AAK1-Antibody-4B10-Sandwich-ELISA-H00022848-M02-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Sperm Flagellar 2 Antibody (4B10) [H00079925-M01] - Analysis of monoclonal antibody to SPEF2 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Sperm-Flagellar-2-Antibody-4B10-Immunocytochemistry-Immunofluorescence-H00079925-M01-img0002.jpg "Immunocytochemistry/Immunofluorescence: Sperm Flagellar 2 Antibody (4B10) [H00079925-M01] - Analysis of monoclonal antibody to SPEF2 on HeLa cell . Antibody concentration 10 ug/ml.")
![Western Blot: STK25 Antibody (4B10) [H00010494-M02] - STK25 monoclonal antibody (M02), clone 4B10 Analysis of STK25 expression in K-562.](http://images.novusbio.com/fullsize/STK25-Antibody-4B10-Western-Blot-H00010494-M02-img0003.jpg "Western Blot: STK25 Antibody (4B10) [H00010494-M02] - STK25 monoclonal antibody (M02), clone 4B10 Analysis of STK25 expression in K-562.")
![Western Blot: STK25 Antibody (4B10) [H00010494-M02] - Analysis of STK25 expression in transfected 293T cell line by STK25 monoclonal antibody (M02), clone 4B10.Lane 1: STK25 transfected lysate (Predicted MW: 48.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/STK25-Antibody-4B10-Western-Blot-H00010494-M02-img0004.jpg)
![Immunoprecipitation: STK25 Antibody (4B10) [H00010494-M02] - Analysis of STK25 transfected lysate using anti-STK25 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with STK25 monoclonal antibody.](http://images.novusbio.com/fullsize/STK25-Antibody-4B10-Immunoprecipitation-H00010494-M02-img0002.jpg)