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![Western Blot: GFAP Antibody (012) [NBP2-89203] - Lane A: Mouse brain tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight 800) at 1:10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 50 kDa. Observed band size: 45 kDa.](http://images.novusbio.com/fullsize/GFAP-Antibody-012-Western-Blot-NBP2-89203-img0001.jpg "Western Blot: GFAP Antibody (012) [NBP2-89203] - Lane A: Mouse brain tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight 800) at 1:10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 50 kDa. Observed band size: 45 kDa.")
![Western Blot: MCAM/CD146 Antibody (012) [NBP2-89202] - Lane A: CD146 konckout Hela Whole Cell Lysate Lane B: Hela Whole Cell LysateLysates/proteins at 10 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:132 kDa Observed band size: kDa](http://images.novusbio.com/fullsize/MCAM-CD146-Antibody-012-Western-Blot-NBP2-89202-img0005.jpg "Western Blot: MCAM/CD146 Antibody (012) [NBP2-89202] - Lane A: CD146 konckout Hela Whole Cell Lysate Lane B: Hela Whole Cell LysateLysates/proteins at 10 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:132 kDa Observed band size: kDa")
![Immunohistochemistry-Paraffin: MCAM/CD146 Antibody (012) [NBP2-89202] - Staining of human kidney showing membrane staining of endothelial cells (red arrow) (1:200).](http://images.novusbio.com/fullsize/MCAM-CD146-Antibody-012-Immunohistochemistry-Paraffin-NBP2-89202-img0003.jpg)
![Immunohistochemistry-Paraffin: MCAM/CD146 Antibody (012) [NBP2-89202] - Staining of human stomach showing membrane staining of endothelial cells (red arrow), epithelial cells (green arrow). The left panel: 1:200. The right panel: 1:1000.](http://images.novusbio.com/fullsize/MCAM-CD146-Antibody-012-Immunohistochemistry-Paraffin-NBP2-89202-img0001.jpg)
![Immunohistochemistry-Paraffin: MCAM/CD146 Antibody (012) [NBP2-89202] - Staining of human lymphonode showing membrane staining of endothelial cells (red arrow), smooth muscle cells (black arrow) (1:200).](http://images.novusbio.com/fullsize/MCAM-CD146-Antibody-012-Immunohistochemistry-Paraffin-NBP2-89202-img0002.jpg)
![Western Blot: GIF Antibody (012) [NBP2-90226] - Anti-GIF rabbit monoclonal antibody at 1:500 dilution. Lane A: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane.](http://images.novusbio.com/fullsize/GIF-Antibody-012-Western-Blot-NBP2-90226-img0004.jpg "Western Blot: GIF Antibody (012) [NBP2-90226] - Anti-GIF rabbit monoclonal antibody at 1:500 dilution. Lane A: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane.")
![Immunoprecipitation: GIF Antibody (012) [NBP2-90226] - GIF was immunoprecipitated using: Lane A:0.5 mg K562 Whole Cell Lysate. Lane B:0.5 mg Jurkat Whole Cell Lysate. 0.5 uL anti-GIF rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-GIF rabbit monoclonal antibody,at 1:500 dilution. Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 45 kDa. Observed band size: 45 kDa.](http://images.novusbio.com/fullsize/GIF-Antibody-012-Immunoprecipitation-NBP2-90226-img0002.jpg)
![Immunoprecipitation: GIF Antibody (012) [NBP2-90226] - GIF was immunoprecipitated using: Lane A:0.5 mg K562 Whole Cell Lysate. Lane B:0.5 mg Jurkat Whole Cell Lysate. 0.5 uL anti-GIF rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-GIF rabbit monoclonal antibody,at 1:500 dilution. Secondary antibody:](http://images.novusbio.com/fullsize/GIF-Antibody-012-Immunoprecipitation-NBP2-90226-img0003.jpg)
![Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody (012) [NBP2-90139] - Immunochemical staining of human SOD2 in human breast-carcinoma with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-012-Immunohistochemistry-Paraffin-NBP2-90139-img0004.jpg "Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody (012) [NBP2-90139] - Immunochemical staining of human SOD2 in human breast-carcinoma with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody (012) [NBP2-90139] - Immunochemical staining of human SOD2 in human placenta with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-012-Immunohistochemistry-Paraffin-NBP2-90139-img0001.jpg)
![Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody (012) [NBP2-90139] - Immunochemical staining of human SOD2 in human kidney with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-012-Immunohistochemistry-Paraffin-NBP2-90139-img0002.jpg)
![Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody (012) [NBP2-90139] - Immunochemical staining of human SOD2 in human colon-carcinoma with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-012-Immunohistochemistry-Paraffin-NBP2-90139-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: CD27/TNFRSF7 Antibody (012) [NBP2-90423] - Immunofluorescence staining of Mouse CD27/TNFRSF7 in Mouse Spleen cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse CD27/TNFRSF7 monoclonal antibody (1:60) at 37C 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cell membrane.](http://images.novusbio.com/fullsize/CD27-TNFRSF7-Antibody-012-Immunocytochemistry-Immunofluorescence-NBP2-90423-img0003.jpg "Immunocytochemistry/Immunofluorescence: CD27/TNFRSF7 Antibody (012) [NBP2-90423] - Immunofluorescence staining of Mouse CD27/TNFRSF7 in Mouse Spleen cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse CD27/TNFRSF7 monoclonal antibody (1:60) at 37C 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cell membrane.")
![Flow Cytometry: CD27/TNFRSF7 Antibody (012) [NBP2-90423] - Flow cytometric analysis of Mouse IFN-alpha/beta R1 expression on BABL/c splenocytes. Cells were stained with Mouse BD Fc Block(TM) purified anti-mouse CD16/CD32 mAb, then stained with purified anti-Mouse IFN-alpha/beta R1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/CD27-TNFRSF7-Antibody-012-Flow-Cytometry-NBP2-90423-img0002.jpg)
![Flow Cytometry: CD27/TNFRSF7 Antibody (012) [NBP2-90423] - Expression of CD27/TNFRSF7 on mouse splenocytes. BABL/c splenocytes were stained with purified mAb R012, then a FITC-conjugated second step antibody . Flow cytometry was performed on a BD FACSCalibur(TM) flow cytometry system.](http://images.novusbio.com/fullsize/CD27-TNFRSF7-Antibody-012-Flow-Cytometry-NBP2-90423-img0001.jpg)
![Western Blot: AARSD1 Antibody (012) [NBP2-90276] - Anti-AARSD1 rabbit monoclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: A549 Whole Cell Lysate. Lysates/proteins at 30 µg per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Performed under reducing conditions. Predicted band size: 45 kDa, Observed band size: 50 kDa.](http://images.novusbio.com/fullsize/AARSD1-Antibody-012-Western-Blot-NBP2-90276-img0001.jpg "Western Blot: AARSD1 Antibody (012) [NBP2-90276] - Anti-AARSD1 rabbit monoclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: A549 Whole Cell Lysate. Lysates/proteins at 30 µg per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Performed under reducing conditions. Predicted band size: 45 kDa, Observed band size: 50 kDa.")
![Immunocytochemistry/Immunofluorescence: IGF-I R/IGF1R Antibody (012) [NBP2-89306] - Staining of Human IGF1R in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human IGF1R monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor (R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cell membrane.](http://images.novusbio.com/fullsize/IGF-I-R-IGF1R-Antibody-012-Immunocytochemistry-Immunofluorescence-NBP2-89306-img0001.jpg "Immunocytochemistry/Immunofluorescence: IGF-I R/IGF1R Antibody (012) [NBP2-89306] - Staining of Human IGF1R in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human IGF1R monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor (R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cell membrane.")
![Flow Cytometry: IGF-I R/IGF1R Antibody (012) [NBP2-89306] - Analysis of human IGF1R expression on HeLa cells. Cells were stained with purified anti-Human IGF1R, then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/IGF-I-R-IGF1R-Antibody-012-Flow-Cytometry-NBP2-89306-img0002.jpg)
![Western Blot: Niemann-Pick type C2 Antibody (012) [NBP2-90209] - Anti-NPC2 rabbit monoclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: NIH-3T3 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C2-Antibody-012-Western-Blot-NBP2-90209-img0004.jpg "Western Blot: Niemann-Pick type C2 Antibody (012) [NBP2-90209] - Anti-NPC2 rabbit monoclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: NIH-3T3 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique.")
![Immunoprecipitation: Niemann-Pick type C2 Antibody (012) [NBP2-90209] - NPC2-His was immunoprecipitated using: Lane A:0.5 mg NIH-3T3 Whole Cell Lysate. 2 uL anti-NPC2-His rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-NPC2-His rabbit monoclonal antibody,at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C2-Antibody-012-Immunoprecipitation-NBP2-90209-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: FcgR4/CD16-2 Antibody (012) [NBP3-05890] - Staining of Mouse FcGR4 in RAW264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Mouse FcGR4 monoclonal antibody (1:100) at 4? overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.](http://images.novusbio.com/fullsize/FcgR4-CD16-2-Antibody-012-Immunocytochemistry-Immunofluorescence-NBP3-05890-img0001.jpg "Immunocytochemistry/Immunofluorescence: FcgR4/CD16-2 Antibody (012) [NBP3-05890] - Staining of Mouse FcGR4 in RAW264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Mouse FcGR4 monoclonal antibody (1:100) at 4? overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.")
![Flow Cytometry: FcgR4/CD16-2 Antibody (012) [NBP3-05890] - Analysis of FCGR4 expression on Raw264.7 cells. Mouse leukaemic monocyte macrophage cell line RAW 264.7 were stained with anti-mouse FCGR4 then a FITC-conjugated second step antibody. The histogram were derived from the gated events based on light scattering characteristics of lymphocytes.](http://images.novusbio.com/fullsize/FcgR4-CD16-2-Antibody-012-Flow-Cytometry-NBP3-05890-img0003.jpg)
![Immunohistochemistry-Paraffin: VE-Cadherin Antibody (012) [NBP2-89497] - Staining of human Cad5 in human kidney with rabbit monoclonal antibody (1:500).](http://images.novusbio.com/fullsize/VE-Cadherin-Antibody-012-Immunohistochemistry-Paraffin-NBP2-89497-img0002.jpg "Immunohistochemistry-Paraffin: VE-Cadherin Antibody (012) [NBP2-89497] - Staining of human Cad5 in human kidney with rabbit monoclonal antibody (1:500).")
![Immunohistochemistry-Paraffin: VE-Cadherin Antibody (012) [NBP2-89497] - Staining of human Cad5 in human stomach with rabbit monoclonal antibody (1:500).](http://images.novusbio.com/fullsize/VE-Cadherin-Antibody-012-Immunohistochemistry-Paraffin-NBP2-89497-img0001.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody (012) [NBP2-90352] - Staining of mouse CA12 in mouse intestine with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-XII-CA12-Antibody-012-Immunohistochemistry-Paraffin-NBP2-90352-img0002.jpg "Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody (012) [NBP2-90352] - Staining of mouse CA12 in mouse intestine with rabbit monoclonal antibody (1:200).")
![Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody (012) [NBP2-90352] - Staining of mouse CA12 in mouse kidney with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-XII-CA12-Antibody-012-Immunohistochemistry-Paraffin-NBP2-90352-img0001.jpg)
![Western Blot: NKp30/NCR3 Antibody (012) [NBP2-89525] - Lane A: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:22 kDa Observed band size:14 kDa](http://images.novusbio.com/fullsize/NKp30-NCR3-Antibody-012-Western-Blot-NBP2-89525-img0001.jpg "Western Blot: NKp30/NCR3 Antibody (012) [NBP2-89525] - Lane A: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:22 kDa Observed band size:14 kDa")