| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-139SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20129-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20145-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20149-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20127-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20133-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20093-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20101-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20107-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20106-0.025mg | |||
![Knockdown Validated: EGLN3/PHD3 Antibody [NB100-139] - PHD3 is required for SCC cell survival in prolonged hypoxia. PHD3 is induced in hypoxia. Knock-down of PHD3 expression with siRNA (siPHD3) is specific and does not affect PHD2 or HIF-1 alpha expression. siScr = scrambled control siRNA. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0014617), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/EGLN3-PHD3-Antibody-Knockdown-Validated-NB100-139-img0017.jpg "<b>Genetic Strategies Validation. </b>Knockdown Validated: EGLN3/PHD3 Antibody [NB100-139] - PHD3 is required for SCC cell survival in prolonged hypoxia. PHD3 is induced in hypoxia. Knock-down of PHD3 expression with siRNA (siPHD3) is specific and does not affect PHD2 or HIF-1 alpha expression. siScr = scrambled control siRNA. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0014617), licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: EGLN3/PHD3 Antibody [NB100-139] - Analysis of a formalin fixed tissue section of human renal cell carcinoma (clear cell type) using rabbit polyclonal EGLN3/PHD3 antibody with HRP-DAB detection and hematoxylin counterstaining. The antibody generated a strong nuclear staining of PHD3 primarily in the cancer cells while the stromal cells were largely negative for this protein.](http://images.novusbio.com/fullsize/EGLN3-PHD3-Antibody-Immunohistochemistry-Paraffin-NB100-139-img0010.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: EGLN3/PHD3 Antibody [NB100-139] - EGLN3/PHD3 depletion increases S10 phosphorylation of p27 in hypoxia. S10 phosphorylated form of p27 is strongly induced in EGLN3/PHD3 depleted cells. Image collected and cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/s12943-015-0410-5) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/EGLN3-PHD3-Antibody-Knockdown-Validated-NB100-139-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: EGLN3/PHD3 Antibody [NB100-139] - EGLN3/PHD3 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/EGLN3-PHD3-Antibody-Immunocytochemistry-Immunofluorescence-NB100-139-img0014.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: EGLN3/PHD3 Antibody [NB100-139] - BxPC3 cells stably transduced with retrovirus containing PHD3Wt (BxPC3-Wt), Vector (BxPC3-Vec) or anti-PHD3 shRNA (BxPC3-KD) were harvested for RNA and protein following 24 hours exposure to normoxia (21% O2) or hypoxia (1% O2). Whole cell lysate from BxPC3-Wt, BxPC3-Vec and BxPC3-KD cells following 24 hours exposure to normoxia (N) or hypoxia (H) was resolved by SDS-PAGE and blotted for actin (top) and PHD3 (bottom). PHD3 band intensity was quantified relative to actin in each lane and then normalized to BxPC3-Vec (N). Relative band intensity is indicated below the figure. NS = non-specific band running just above PHD3 band. Data is representative of >3 biological replicates. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0083021), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/EGLN3-PHD3-Antibody-Knockdown-Validated-NB100-139-img0016.jpg)
![Western Blot: EGLN3/PHD3 Antibody [NB100-139] - The HIF-1 response is not impaired in prostate and breast carcinoma cell lines that lack PHD3. Melanoma, breast and prostate cancer cell lines with methylated PHD3 promoters (MB-435, PC-3) and non-methylated promoters (MCF7, DU 145) were subjected to hypoxia (1% O2) or normoxia for 24 hours. Thirty micrograms of whole cell lysate was western blotted for the presence of PHD3; actin was used as a loading control. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0014617), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/EGLN3-PHD3-Antibody-Western-Blot-NB100-139-img0018.jpg)
![Western Blot: EGLN3/PHD3 Antibody [NB100-139] - Analysis of HeLa lysates using NB100-139. Image courtesy of Dr Gregg Semenza (The Johns Hopkins University School of Medicine, Baltimore, MD USA)](http://images.novusbio.com/fullsize/EGLN3-PHD3-Antibody-Western-Blot-NB100-139-img0013.jpg)
![SDS-Page: Recombinant Human DRP1 Protein [H00010059-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-DRP1-Protein-SDS-Page-H00010059-P01-img0002.jpg "SDS-Page: Recombinant Human DRP1 Protein [H00010059-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![SDS-Page: Recombinant Human Actin Gamma 1 Protein [H00000071-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-Actin-Gamma-1-Protein-SDS-Page-H00000071-P01-img0001.jpg "SDS-Page: Recombinant Human Actin Gamma 1 Protein [H00000071-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunocytochemistry/Immunofluorescence: CBX1 Antibody (RAB-C145) - Chimeric - Azide and BSA Free [NBP3-20129] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of RAB-C145 (NBP3-20129) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right, top-bottom NBP3-20129, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20129_rabbit-cbx1-mab-rab-c145-chimeric-azide-and-bsa-free-2742023924272.png "Immunocytochemistry/Immunofluorescence: CBX1 Antibody (RAB-C145) - Chimeric - Azide and BSA Free [NBP3-20129] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of RAB-C145 (NBP3-20129) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right, top-bottom NBP3-20129, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![Western Blot: CBX1 Antibody (RAB-C145) - Chimeric - Azide and BSA Free [NBP3-20129] - Nuclear lysates of HeLa(A) (0.3ug/ml), HEK293(B) (0.3ug/ml) and MCF7(C) (0.1ug/ml) (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of RAB-C145 (NBP3-20129) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20129_rabbit-cbx1-mab-rab-c145-chimeric-azide-and-bsa-free-2742023924257.png)
![Flow Cytometry: CBX1 Antibody (RAB-C145) - Chimeric - Azide and BSA Free [NBP3-20129] - HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG1 version of RAB-C145 (NBP3-20129, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20129_rabbit-cbx1-mab-rab-c145-chimeric-azide-and-bsa-free-274202392429.png)
![Flow Cytometry: SETD7 Antibody (RAB-C220) - Chimeric - Azide and BSA Free [NBP3-20145] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of RAB-C220 (NBP3-20145, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20145_rabbit-setd7-mab-rab-c220-chimeric-azide-and-bsa-free-2742023924289.png "Flow Cytometry: SETD7 Antibody (RAB-C220) - Chimeric - Azide and BSA Free [NBP3-20145] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of RAB-C220 (NBP3-20145, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.")
![Western Blot: SETD7 Antibody (RAB-C220) - Chimeric - Azide and BSA Free [NBP3-20145] - Nuclear lysate of MCF7(A) (0.01ug/ml), Jurkat(B) (0.1ug/ml) cells and A549(C) (0.03ug/ml) cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of RAB-C220 (NBP3-20145) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20145_rabbit-setd7-mab-rab-c220-chimeric-azide-and-bsa-free-2742023924219.png)
![Immunocytochemistry/Immunofluorescence: SETD7 Antibody (RAB-C220) - Chimeric - Azide and BSA Free [NBP3-20145] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of RAB-C220 (NBP3-20145) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1500 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20145, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20145_rabbit-setd7-mab-rab-c220-chimeric-azide-and-bsa-free-2742023924216.png)
![Western Blot: TDRD3 Antibody (RAB-C419) - Chimeric - Azide and BSA Free [NBP3-20149] - Human brain cerebellum(A) (1ug/ml), human skeletal muscle(B) (0.3ug/ml), heart(C) (0.3ug/ml), human placenta(D) (0.1ug/ml), human testes(E) (0.3ug/ml) and human kidney(F) (0.1ug/ml) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of RAB-C419 (NBP3-20149) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20149_rabbit-tdrd3-mab-rab-c419-chimeric-azide-and-bsa-free-2742023924245.png "Western Blot: TDRD3 Antibody (RAB-C419) - Chimeric - Azide and BSA Free [NBP3-20149] - Human brain cerebellum(A) (1ug/ml), human skeletal muscle(B) (0.3ug/ml), heart(C) (0.3ug/ml), human placenta(D) (0.1ug/ml), human testes(E) (0.3ug/ml) and human kidney(F) (0.1ug/ml) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of RAB-C419 (NBP3-20149) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Flow Cytometry: TDRD3 Antibody (RAB-C419) - Chimeric - Azide and BSA Free [NBP3-20149] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of RAB-C419 (NBP3-20149, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20149_rabbit-tdrd3-mab-rab-c419-chimeric-azide-and-bsa-free-2742023924220.png)
![Immunocytochemistry/Immunofluorescence: TDRD3 Antibody (RAB-C419) - Chimeric - Azide and BSA Free [NBP3-20149] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of RAB-C419 (NBP3-20149) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1500 dilution), showing nucleoli, nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20149, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20149_rabbit-tdrd3-mab-rab-c419-chimeric-azide-and-bsa-free-2742023924290.png)
![Flow Cytometry: HP1 alpha Antibody (RAB-C133) - Chimeric - Azide and BSA Free [NBP3-20127] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of RAB-C133 (NBP3-20127, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20127_rabbit-hp1-alpha-mab-rab-c133-chimeric-azide-bsa-free-2742023924265.png "Flow Cytometry: HP1 alpha Antibody (RAB-C133) - Chimeric - Azide and BSA Free [NBP3-20127] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of RAB-C133 (NBP3-20127, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.")
![Western Blot: HP1 alpha Antibody (RAB-C133) - Chimeric - Azide and BSA Free [NBP3-20127] - Nuclear lysates of HeLa(A), HEK293(B), A431(C), MCF7(D), Jurkat(E) and K562(F) cells (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of RAB-C133 (NBP3-20127) at 0.003 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20127_rabbit-hp1-alpha-mab-rab-c133-chimeric-azide-bsa-free-2742023924261.png)
![Immunocytochemistry/Immunofluorescence: HP1 alpha Antibody (RAB-C133) - Chimeric - Azide and BSA Free [NBP3-20127] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of RAB-C133 (NBP3-20127) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20127, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20127_rabbit-hp1-alpha-mab-rab-c133-chimeric-azide-bsa-free-2742023924264.png)
![Flow Cytometry: WDR5 Antibody (RAB-C223) - Chimeric - Azide and BSA Free [NBP3-20133] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of RAB-C223 (NBP3-20133, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20133_rabbit-wdr5-mab-rab-c223-chimeric-azide-and-bsa-free-2742023924296.png "Flow Cytometry: WDR5 Antibody (RAB-C223) - Chimeric - Azide and BSA Free [NBP3-20133] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of RAB-C223 (NBP3-20133, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.")
![Western Blot: WDR5 Antibody (RAB-C223) - Chimeric - Azide and BSA Free [NBP3-20133] - Nuclear lysate of MCF7 cells (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of RAB-C223 (NBP3-20133) at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20133_rabbit-wdr5-mab-rab-c223-chimeric-azide-and-bsa-free-2742023924242.png)
![Immunocytochemistry/Immunofluorescence: WDR5 Antibody (RAB-C223) - Chimeric - Azide and BSA Free [NBP3-20133] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of RAB-C223 (NBP3-20133) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1500 dilution), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20133, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20133_rabbit-wdr5-mab-rab-c223-chimeric-azide-and-bsa-free-2742023964611.png)
![Flow Cytometry: Annexin A4 Antibody (SAIC-14C-10F12) - Azide and BSA Free [NBP3-20093] - Paraformaldehyde fixed HepG2 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-14C-10F12 (NBP3-20093, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20093_rabbit-annexin-a4-mab-saic-14c-10f12-azide-and-bsa-free-2742023924238.png "Flow Cytometry: Annexin A4 Antibody (SAIC-14C-10F12) - Azide and BSA Free [NBP3-20093] - Paraformaldehyde fixed HepG2 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-14C-10F12 (NBP3-20093, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.")
![Western Blot: Annexin A4 Antibody (SAIC-14C-10F12) - Azide and BSA Free [NBP3-20093] - HepG2(A) (0.001ug/ml), A431(B) (0.001ug/ml), and HeLa(C) (0.01ug/ml) cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-14C-10F12 (NBP3-20093), before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20093_rabbit-annexin-a4-mab-saic-14c-10f12-azide-and-bsa-free-274202396462.png)
![Immunocytochemistry/Immunofluorescence: Annexin A4 Antibody (SAIC-14C-10F12) - Azide and BSA Free [NBP3-20093] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-14C-10F12 (NBP3-20093) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20093, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20093_rabbit-annexin-a4-mab-saic-14c-10f12-azide-and-bsa-free-2742023924225.png)
![Immunocytochemistry/Immunofluorescence: Transgelin/TAGLN/SM22 alpha Antibody (SAIC-33B-129) - Azide and BSA Free [NBP3-20101] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-33B-129 (NBP3-20101) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20101, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20101_rabbit-transgelin-tagln-sm22-alpha-mab-saic-33b-129-azide-bsa-free-2742023924294.png "Immunocytochemistry/Immunofluorescence: Transgelin/TAGLN/SM22 alpha Antibody (SAIC-33B-129) - Azide and BSA Free [NBP3-20101] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-33B-129 (NBP3-20101) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20101, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![Western Blot: Transgelin/TAGLN/SM22 alpha Antibody (SAIC-33B-129) - Azide and BSA Free [NBP3-20101] - HeLa(A), Caco-2(B), U2OS(C) cell lysate and human placenta(D), muscle(E) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-33B-129 (NBP3-20101) at 0.0003 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20101_rabbit-transgelin-tagln-sm22-alpha-mab-saic-33b-129-azide-bsa-free-2742023924271.png)
![Flow Cytometry: Transgelin/TAGLN/SM22 alpha Antibody (SAIC-33B-129) - Azide and BSA Free [NBP3-20101] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-33B-129 (NBP3-20101, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20101_rabbit-transgelin-tagln-sm22-alpha-mab-saic-33b-129-azide-bsa-free-274202396468.png)
![Western Blot: UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free [NBP3-20107] - Caco-2(A) (0.3ug/ml), MCF7(B) (1ug/ml) and HeLa(C) (1ug/ml) cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-41A-1 (NBP3-20107) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20107_rabbit-ubch10-ube2c-mab-saic-41a-1-azide-and-bsa-free-2742023924254.png "Western Blot: UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free [NBP3-20107] - Caco-2(A) (0.3ug/ml), MCF7(B) (1ug/ml) and HeLa(C) (1ug/ml) cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-41A-1 (NBP3-20107) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Flow Cytometry: UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free [NBP3-20107] - Paraformaldehyde fixed MCF7 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-41A-1 (NBP3-20107, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20107_rabbit-ubch10-ube2c-mab-saic-41a-1-azide-and-bsa-free-2742023964610.png)
![Immunocytochemistry/Immunofluorescence: UbcH10/UBE2C Antibody (SAIC-41A-1) - Azide and BSA Free [NBP3-20107] - Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-41A-1 (NBP3-20107) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing NUCLEAR staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20107, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20107_rabbit-ubch10-ube2c-mab-saic-41a-1-azide-and-bsa-free-2742023924231.png)
![Immunocytochemistry/Immunofluorescence: Peroxiredoxin 4 Antibody (SAIC-40C-8) - Azide and BSA Free [NBP3-20106] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-40C-8 (NBP3-20106) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20106, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20106_rabbit-peroxiredoxin-4-mab-saic-40c-8-azide-and-bsa-free-2742023924227.png "Immunocytochemistry/Immunofluorescence: Peroxiredoxin 4 Antibody (SAIC-40C-8) - Azide and BSA Free [NBP3-20106] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-40C-8 (NBP3-20106) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20106, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![Flow Cytometry: Peroxiredoxin 4 Antibody (SAIC-40C-8) - Azide and BSA Free [NBP3-20106] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-40C-8 (NBP3-20106, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20106_rabbit-peroxiredoxin-4-mab-saic-40c-8-azide-and-bsa-free-2742023924260.png)
![Western Blot: Peroxiredoxin 4 Antibody (SAIC-40C-8) - Azide and BSA Free [NBP3-20106] - HEK293 cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-40C-8 (NBP3-20106) at 0.001 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20106_rabbit-peroxiredoxin-4-mab-saic-40c-8-azide-and-bsa-free-2742023924214.png)