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![Immunohistochemistry: CAT1 Antibody [NBP3-35470] - Immunohistochemistry analysis of paraffin-embedded Rat testis using CAT1 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35470_rabbit-cat1-pab-101220241136067.jpg "Immunohistochemistry: CAT1 Antibody [NBP3-35470] - Immunohistochemistry analysis of paraffin-embedded Rat testis using CAT1 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Immunohistochemistry: CAT1 Antibody [NBP3-35470] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using CAT1 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35470_rabbit-cat1-pab-1012202411392941.jpg)
![Western Blot: CAT1 Antibody [NBP3-35470] - Western blot analysis of lysates from LNCaP cells, using CAT1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-35470_rabbit-cat1-pab-1012202411364610.jpg)
![Western Blot: CAT1 Antibody (2B9) [H00006541-M02] - Since miRNA214 is upregulated and miRNA 378 is downregulated following 12 days of TAC, western immunoblotting was carried out for nodal molecule (NF kappa B) and other network molecules (HDGF, BCL2 and SLC7A1). GAPDH was used a loading control, *p<0.05 vs. Sham, (n = 8). Image collected and cropped by CiteAb from the following publication (journals.plos.org/plosone/article?id=10.1371/journal.pone.0170456), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CAT1-Antibody-2B9-Western-Blot-H00006541-M02-img0003.jpg "Western Blot: CAT1 Antibody (2B9) [H00006541-M02] - Since miRNA214 is upregulated and miRNA 378 is downregulated following 12 days of TAC, western immunoblotting was carried out for nodal molecule (NF kappa B) and other network molecules (HDGF, BCL2 and SLC7A1). GAPDH was used a loading control, *p<0.05 vs. Sham, (n = 8). Image collected and cropped by CiteAb from the following publication (journals.plos.org/plosone/article?id=10.1371/journal.pone.0170456), licensed under a CC-BY license.")
![Western Blot: CAT1 Antibody (2B9) [H00006541-M02] - Analysis of SLC7A1 expression in transfected 293T cell line by SLC7A1 monoclonal antibody (M02), clone 2B9.Lane 1: SLC7A1 transfected lysate (Predicted MW: 67.6 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CAT1-Antibody-2B9-Western-Blot-H00006541-M02-img0002.jpg)
![Sandwich ELISA: CAT1 Antibody (2B9) [H00006541-M02] - Detection limit for recombinant GST tagged SLC7A1 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CAT1-Antibody-2B9-Sandwich-ELISA-H00006541-M02-img0001.jpg)
![Western Blot: CAT1 Antibody (2B9) [H00006541-M02] - A, Since miRNA214 is upregulated & miRNA 378 is downregulated following 12 days of TAC, western immunoblotting was carried out for nodal molecule (NF kappa B) & other network molecules (HDGF, BCL2 & SLC7A1). GAPDH was used a loading control, *p<0.05 vs. Sham, (n = 8). B, HL-1 cardiomyocyte cells were transfected with allstar scrambled control (Vec), or miRNA 214 along with miRNA 378 or miRNA 214 along with antagomir for miRNA 378. The cells were lysed & subjected to immunoblotting for NF kappa B. GAPDH was used as loading control. *p<0.05 vs. Vec (scrambled vector control transfected cells); **p<0.05 vs. miRNA 214 & 378 transfected cells (n = 3). C, HEK 293 cells were transfected with Vec or miRNA 214 along with miRNA 378 or miRNA 214 along with antagomir for miRNA 378. The cell lysates were subjected to immunoblotting for NF kappa B. GAPDH was used as loading control. *p<0.05 vs. Vec; **p<0.05 vs. miRNA 214 & 378 transfected cells (n = 3). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28329018), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00006541-m02_mouse-monoclonal-cat1-antibody-2b9-310202415482217.jpg)
![Western Blot: CAT1 Antibody [NBP1-90066] - Analysis in human cell line SCLC-21H.](http://images.novusbio.com/fullsize/CAT1-Antibody-Western-Blot-NBP1-90066-img0009.jpg "Western Blot: CAT1 Antibody [NBP1-90066] - Analysis in human cell line SCLC-21H.")
![Western Blot: CAT1 Antibody [NBP1-59857] - Reccomended Titration: 1 ug/mL Sample Type: Human MDA-MB-435s](http://images.novusbio.com/fullsize/CAT1-Antibody-Western-Blot-NBP1-59857-img0004.jpg "Western Blot: CAT1 Antibody [NBP1-59857] - Reccomended Titration: 1 ug/mL Sample Type: Human MDA-MB-435s")
![Western Blot: CAT1 Antibody [NBP1-59857] - Jurkat cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/CAT1-Antibody-Western-Blot-NBP1-59857-img0002.jpg)
![Western Blot: CAT1 Antibody [NBP1-59857] - Antibody Titration: 1 ug/ml Human 721_B.](http://images.novusbio.com/fullsize/CAT1-Antibody-Western-Blot-NBP1-59857-img0003.jpg)
![Western Blot: CAT1 Antibody [NBP2-32271] - Samples: Whole cell lysate (50 ug) prepared using RIPA buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-CAT-1 antibody NBP2-32271 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CAT1-Antibody-Western-Blot-NBP2-32271-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: CAT1 Antibody [NBP2-32271] - Samples: Whole cell lysate (50 ug) prepared using RIPA buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-CAT-1 antibody NBP2-32271 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: CAT1 Antibody [NBP2-32271] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using RIPA buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-CAT-1 antibody NBP2-32271 used for IP at 6 ug per reaction. CAT-1 was also immunoprecipitated by rabbit anti-CAT-1 antibody BL16464. For blotting immunoprecipitated CAT-1, NBP2-32271 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/CAT1-Antibody-Immunoprecipitation-NBP2-32271-img0002.jpg)
![Immunohistochemistry-Paraffin: CAT1 Antibody [NBP3-17813] - Staining of human pancreas shows strong membranous positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/CAT1-Antibody-Immunohistochemistry-Paraffin-NBP3-17813-img0001.jpg "Immunohistochemistry-Paraffin: CAT1 Antibody [NBP3-17813] - Staining of human pancreas shows strong membranous positivity in exocrine glandular cells.")
![Western Blot: SLC7A2 Overexpression Lysate (Adult Normal) [NBP2-08685] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SLC7A2.](http://images.novusbio.com/fullsize/SLC7A2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-08685-img0001.jpg "Western Blot: SLC7A2 Overexpression Lysate (Adult Normal) [NBP2-08685] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SLC7A2.")
![Western Blot: CAT1 Antibody [NBP1-59836] - OVCAR-3 cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/CAT1-Antibody-Western-Blot-NBP1-59836-img0002.jpg "Western Blot: CAT1 Antibody [NBP1-59836] - OVCAR-3 cell lysate, concentration 0.2-1 ug/ml.")