| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1252AF405 | |||
| Alexa Fluor 488 | NB21-1252AF488 | |||
| Alexa Fluor 647 | NB21-1252AF647 | |||
| Alexa Fluor 700 | NB21-1252AF700 | |||
| Biotin | NB21-1252B | |||
| DyLight 405 | NB21-1252V | |||
| DyLight 488 | NB21-1252G | |||
| DyLight 550 | NB21-1252R | |||
| DyLight 650 | NB21-1252C | |||
| FITC | NB21-1252F | |||
| HRP | NB21-1252H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1252SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1251AF405 | |||
| Alexa Fluor 488 | NB21-1251AF488 | |||
| Alexa Fluor 647 | NB21-1251AF647 | |||
| Alexa Fluor 700 | NB21-1251AF700 | |||
| Biotin | NB21-1251B | |||
| DyLight 405 | NB21-1251V | |||
| DyLight 488 | NB21-1251G | |||
| DyLight 550 | NB21-1251R | |||
| DyLight 650 | NB21-1251C | |||
| FITC | NB21-1251F | |||
| HRP | NB21-1251H | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1253AF405 | |||
| Alexa Fluor 488 | NB21-1253AF488 | |||
| Alexa Fluor 647 | NB21-1253AF647 | |||
| Alexa Fluor 700 | NB21-1253AF700 | |||
| Biotin | NB21-1253B | |||
| DyLight 405 | NB21-1253V | |||
| DyLight 488 | NB21-1253G | |||
| DyLight 550 | NB21-1253R | |||
| DyLight 650 | NB21-1253C | |||
| HRP | NB21-1253H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1253SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-25988 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-25987 | |||
![Western Blot: Histone H3 [Trimethyl Lys36] Antibody [NBP2-54611] - Histone 3 [Trimethyl Lys36] Antibody [NBP2-54611] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K36me3. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight ~16 kDa.](http://images.novusbio.com/fullsize/Histone-3-[Trimethyl-Lys36]-Antibody-Western-Blot-NBP2-54611-img0001.jpg "Western Blot: Histone H3 [Trimethyl Lys36] Antibody [NBP2-54611] - Histone 3 [Trimethyl Lys36] Antibody [NBP2-54611] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K36me3. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight ~16 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys36] Antibody [NBP2-54611] - Histone 3 [Trimethyl Lys36] Antibody [NBP2-54611] - HeLa cells were stained with the antibody against H3K36me3 and with DAPI. Cells were fixed with 4% formaldehyde for 10 min. and blocked with PBS/Triton X-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K36me3 antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-3-[Trimethyl-Lys36]-Antibody-Immunofluorescence-NBP2-54611-img0003.jpg)
![Chromatin Immunoprecipitation Sequencing: Histone H3 [Trimethyl Lys36] Antibody [NBP2-54611] - ChIP assays were performed using human K562 cells, the antibody against H3K36me3 and optimized PCR primer pairs for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 100,000 cells. A titration consisting of 0.2, 0.5, 1 and 2 ug of antibody per ChIP experiment was analyzed. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the coding region of the active GAPDH and ACTB genes, used as positive controls, and for the coding region of the inactive MB gene and the Sat satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys36]-Antibody-Chromatin-Immunoprecipitation-Sequencing-NBP2-54611-img0015.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys36] Antibody [NBP2-54611] - ChIP assays were performed using human HeLa cells, the antibody against H3K36me3 and optimized PCR primer pairs for qPCR. ChIP was performed with an Histone ChIP-seq kit, using sheared chromatin from 1,000,000 cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the coding region of the active GAPDH and ACTB genes, used as positive controls, and for the promoter and a region located 1 kb upstream of the promoter of the GAPDH gene, used as negative controls.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys36]-Antibody-Chromatin-Immunoprecipitation-NBP2-54611-img0014.jpg)
![Dot Blot: Histone H3 [Trimethyl Lys36] Antibody [NBP2-54611] - Histone 3 [Trimethyl Lys36] Antibody [NBP2-54611] - To test the cross reactivity of the antibody against H3K36me3, a Dot Blot analysis was performed with peptides containing other modifications or unmodified sequences of histone H3 and H4. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. The figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-3-[Trimethyl-Lys36]-Antibody-Dot-Blot-NBP2-54611-img0004.jpg)
![ELISA: Histone H3 [Trimethyl Lys36] Antibody [NBP2-54611] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against H3K36me3. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:132,000.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys36]-Antibody-ELISA-NBP2-54611-img0012.jpg)
![Protein Array: Histone H3 [Trimethyl Lys36] Antibody [NBP2-54611] - The specificity of the antibody was demonstrated by peptide array analyses on an array containing 384 peptides with different combinations of modifications from histone H3, H4, H2A and H2B. The antibody was used at a dilution of 1:10,000. The figure shows the specificity factor, calculated as the ratio of the average intensity of all spots containing the mark, divided by the average intensity of all spots not containing the mark. The peptide array analysis shows a slight cross reaction with H4K20me3 that was not observed in dot blot.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys36]-Antibody-Protein-Array-NBP2-54611-img0013.jpg)
![Dot Blot: Histone H3 [Dimethyl Lys36] Antibody [NB21-1252] - Analysis of H3 K36-Me2 using the peptides stated above in 10, 6, 3 and 1 picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys36]-Antibody-Dot-Blot-NB21-1252-img0010.jpg "Dot Blot: Histone H3 [Dimethyl Lys36] Antibody [NB21-1252] - Analysis of H3 K36-Me2 using the peptides stated above in 10, 6, 3 and 1 picomoles of peptide.")
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys36] Antibody [NB21-1252] - Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2 ug of NB21-1252 and 20 uL of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys36]-Antibody-Chromatin-Immunoprecipitation-NB21-1252-img0009.jpg)
![Western Blot: Histone H3 [Dimethyl Lys36] Antibody [NB21-1252] - Analysis of H3 K36Me2 in C. elegans embryo lysate. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys36]-Antibody-Western-Blot-NB21-1252-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys36] Antibody [NB21-1252] - Antibody was tested in Neuro2a cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys36]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1252-img0016.jpg)
![Western Blot: Histone H3 [Dimethyl Lys36] Antibody [NB21-1252] - Analysis of H3K36me2 in HeLa histone preps. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys36]-Antibody-Western-Blot-NB21-1252-img0011.jpg)
![Dot Blot: Histone H3 [Monomethyl Lys36] Antibody [NB21-1251] - Analysis of H3 K36me1 using the peptides stated in 10, 6, 3, 1, 0.6 and 0.3 picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys36]-Antibody-Dot-Blot-NB21-1251-img0008.jpg "Dot Blot: Histone H3 [Monomethyl Lys36] Antibody [NB21-1251] - Analysis of H3 K36me1 using the peptides stated in 10, 6, 3, 1, 0.6 and 0.3 picomoles of peptide.")
![Western Blot: Histone H3 [Monomethyl Lys36] Antibody [NB21-1251] - Analysis of Histone H3 K36me1 in C. elegans embryo lysate. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys36]-Antibody-Western-Blot-NB21-1251-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Monomethyl Lys36] Antibody [NB21-1251] - Histone H3 K36me1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys36]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1251-img0013.jpg)
![Western Blot: Histone H3 [Monomethyl Lys36] Antibody [NB21-1251] - Analysis of H3 K36me1 in HeLa histone preps. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys36]-Antibody-Western-Blot-NB21-1251-img0009.jpg)
![Dot Blot: Histone H3 [Trimethyl Lys36] Antibody [NB21-1253] - Analysis of H3K36me3 in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys36]-Antibody-Dot-Blot-NB21-1253-img0002.jpg "Dot Blot: Histone H3 [Trimethyl Lys36] Antibody [NB21-1253] - Analysis of H3K36me3 in picomoles of peptide.")
![Western Blot: Histone H3 [Trimethyl Lys36] Antibody [NB21-1253] - Analysis of Histone H3K36me3 in C. elegans embryo lysate. Observed molecular weight is ~18 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys36]-Antibody-Western-Blot-NB21-1253-img0004.jpg)
![Western Blot: Histone H3 [Trimethyl Lys36] Antibody - BSA Free [NB21-1253] - Detectionof H3K36me3 in NIH/3T3 histone preps. Observed molecular weight is 15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys36]-Antibody---BSA-Free-Western-Blot-NB21-1253-img0005.jpg)
![Western Blot: Histone H3 [Trimethyl Lys36] Antibody [NBP2-59199] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody directed against H3K36me3 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys36]-Antibody-Western-Blot-NBP2-59199-img0001.jpg "Western Blot: Histone H3 [Trimethyl Lys36] Antibody [NBP2-59199] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody directed against H3K36me3 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys36] Antibody [NBP2-59199] - ChIP assays were performed using HeLa cells, the antibody against H3K36me3 and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. QPCR was performed with primers for the promoter and coding region of the active GAPDH, for a region located 1 kb upstream of the GAPDH promoter and for the Sat2 satellite repeat. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys36]-Antibody-Chromatin-Immunoprecipitation-NBP2-59199-img0003.jpg)
![ELISA: Histone H3 [Trimethyl Lys36] Antibody [NBP2-59199] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H3K36me3 and the crude serum. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1:19,300.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys36]-Antibody-ELISA-NBP2-59199-img0002.jpg)
![Dot Blot: Histone H3 [Trimethyl Lys36] Antibody [NBP2-59199] - A Dot Blot analysis was performed to test the cross reactivity of the antibody against H3K36me3 with peptides containing other H3 and H4 modifications and the unmodified sequence. One hundred to 0.2 pmol of peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys36]-Antibody-Dot-Blot-NBP2-59199-img0005.jpg)
![Western Blot: Histone H3 [Methyl Lys36] Antibody [NBP2-59211] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K36me1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Methyl-Lys36]-Antibody-Western-Blot-NBP2-59211-img0001.jpg "Western Blot: Histone H3 [Methyl Lys36] Antibody [NBP2-59211] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K36me1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Methyl Lys36] Antibody [NBP2-59211] - Human osteosarcoma (U2OS) cells were stained with the antibody against H3K36me1 and with DAPI. Cells were fixed with 4% formaldehyde for 20 minutes and blocked with PBS/TX-100 containing 5% normal goat serum. Figure A: cells were immunofluorescently labeled with the H3K36me1 antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 568 or with DAPI (right), which specifically labels DNA. Figure B, C, D and E: staining of the cells with the H3K36me1 antibody after incubation of the antibody with 2 ng/ul blocking peptide containing the unmodified and the mono-, di- and trimethylated H3K36, respectively.](http://images.novusbio.com/fullsize/Histone-H3-[Methyl-Lys36]-Antibody-Immunofluorescence-NBP2-59211-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Methyl Lys36] Antibody [NBP2-59211] - ChIP assays were performed using human U2OS cells, the antibody against H3K36me1 and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1.5 million cells and stringent washing conditions. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoter of the constitutively expressed GAPDH gene and for the inactive gene MYOD. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[Methyl-Lys36]-Antibody-Chromatin-Immunoprecipitation-NBP2-59211-img0003.jpg)
![ELISA: Histone H3 [Methyl Lys36] Antibody [NBP2-59211] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H3K36me1, crude serum and Flow Through. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:46,000.](http://images.novusbio.com/fullsize/Histone-H3-[Methyl-Lys36]-Antibody-ELISA-NBP2-59211-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys36] Antibody (C.15200183) [NBP2-59301] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys36-Antibody-C-15200183-Immunofluorescence-NBP2-59301-img0003.jpg "Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys36] Antibody (C.15200183) [NBP2-59301] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.")
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys36] Antibody (C.15200183) [NBP2-59301] - ChIP assays were performed using human HeLa cells, the antibody against H3K36me3 and optimized PCR primer pairs for qPCR. ChIP was performed with the Auto Histone ChIP-seq kit, using sheared chromatin from 1 million cells. A titration consisting of 0.5, 1, 2 and 5 ug of antibody per ChIP experiment was analyzed. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the coding regions of the active EIF2S3 and CCT5 genes, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys36-Antibody-C-15200183-Chromatin-Immunoprecipitation-NBP2-59301-img0002.jpg)
![ELISA: Histone H3 [Trimethyl Lys36] Antibody (C.15200183) [NBP2-59301] - To test the specificity an ELISA was performed using a serial dilution of the antibody against H3K36me3. The wells were coated with peptides containing the unmodified H3K36 region as well as the mono-, di- and trimethylated H3K36 and the trimethylated H3K9. Figure shows a high specificity of the antibody for the peptide containing the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys36-Antibody-C-15200183-ELISA-NBP2-59301-img0001.jpg)
![Western Blot: Histone H3 [Trimethyl Lys36] Antibody (RM155) [NBP2-61530] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61530 at 2 ug/ml. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys36-Antibody-RM155-Western-Blot-NBP2-61530-img0004.jpg "Western Blot: Histone H3 [Trimethyl Lys36] Antibody (RM155) [NBP2-61530] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61530 at 2 ug/ml. Observed molecular weight is ~16 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys36] Antibody (RM155) [NBP2-61530] - ChIP performed on HeLa cells using H3K36me3 antibody (NBP2-61530). Real-time PCR was performed using primers specific to the gene indicated.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys36-Antibody-RM155-Chromatin-Immunoprecipitation-NBP2-61530-img0006.jpg)
![Multiplex Immunoassay: Histone H3 [Trimethyl Lys36] Antibody (RM155) [NBP2-61530] - NBP2-61530 specifically reacts to Histone H3 trimethylated at Lysine 36 (K36me3). No cross reactivity with non-modified Lysine 36 (K36 Ctrl), monomethylated Lysine 36 (K36me1) or dimethylated Lysine 36 (K36me2), or other methylations in Histone H3.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys36-Antibody-RM155-Multiplex-Immunoassay-NBP2-61530-img0005.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys36] Antibody (C.15200182) [NBP2-59296] - ChIP assays were performed using human HeLa cells, the antibody against H3K36me2 and optimized PCR primer pairs for qPCR. ChIP was performed with the Auto Histone ChIP-seq kit, using sheared chromatin from 1 million cells. A titration consisting of 0.5, 1, 2 and 5 ug of antibody per ChIP experiment was analyzed. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for a genomic region upstream of the TGM2 gene, used as a positive control, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys36-Antibody-C-15200182-Chromatin-Immunoprecipitation-NBP2-59296-img0002.jpg "Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys36] Antibody (C.15200182) [NBP2-59296] - ChIP assays were performed using human HeLa cells, the antibody against H3K36me2 and optimized PCR primer pairs for qPCR. ChIP was performed with the Auto Histone ChIP-seq kit, using sheared chromatin from 1 million cells. A titration consisting of 0.5, 1, 2 and 5 ug of antibody per ChIP experiment was analyzed. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for a genomic region upstream of the TGM2 gene, used as a positive control, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).")
![ELISA: Histone H3 [Dimethyl Lys36] Antibody (C.15200182) [NBP2-59296] - Cross reactivity of the antibody directed against H3K36me2. To test the specificity an ELISA was performed using a serial dilution of the antibody against H3K36me2. The wells were coated with peptides containing the unmodified H3K36 region as well as the mono-, di- and trimethylated H3K36 and the trimethylated H3K9. Figure shows a high specificity of the antibody for the peptide containing the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys36-Antibody-C-15200182-ELISA-NBP2-59296-img0001.jpg)
![Multiplex Immunoassay: Histone H3 [Dimethyl Lys36] Antibody (RM141) [NBP2-61506] - NBP2-61506 specifically reacts to Histone H3 dimethylated at Lysine 36 (K36me2). No cross reactivity with non-modified Lysine 36 (K36 Ctrl), monomethylated Lysine 36 (K36me1), trimethylated Lysine 36 (K36me3), or other methylations in Histone H3.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys36-Antibody-RM141-Multiplex-Immunoassay-NBP2-61506-img0004.jpg "Multiplex Immunoassay: Histone H3 [Dimethyl Lys36] Antibody (RM141) [NBP2-61506] - NBP2-61506 specifically reacts to Histone H3 dimethylated at Lysine 36 (K36me2). No cross reactivity with non-modified Lysine 36 (K36 Ctrl), monomethylated Lysine 36 (K36me1), trimethylated Lysine 36 (K36me3), or other methylations in Histone H3.")
![Western Blot: Histone H3 [Dimethyl Lys36] Antibody (RM141) [NBP2-61506] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61506 at,0.5 ug/ml showed a band of histone H3 dimethylated at Lysine 36 (K36me2) in HeLa cells. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys36-Antibody-RM141-Western-Blot-NBP2-61506-img0003.jpg)