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NB100-317

TERT Antibody (2C4)

(25 Publications)

0.1 ml

NB100-317

Western Blot: TERT Antibody (2C4) [NB100-317] - Increased levels of IFI16 protein in WI-38 cells reduce hTERT levels and inhibit telomerase activity. Total protein extracts prepared from WI-38 cells, either transfected with control (pCMV) vector (lane 1) or pCMV-IFI16 plasmid (lane 2), were subjected to immunoblotting using antibodies specific to the indicated proteins. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0008569), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: TERT Antibody (2C4) [NB100-317] - Human Pancreatic carcinoma showing strong staining of tumor cells.

Flow Cytometry: TERT Antibody (2C4) [NB100-317] - An intracellular stain was performed on A431 cells with TERT Antibody [2C4] NB100-317 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgM (H+L) Cross-Adsorbed Secondary Antibody, AlexaFluor 488.

Western Blot: TERT Antibody (2C4) [NB100-317] - 1:500 dilution on MJ90 cells.

Immunohistochemistry-Paraffin: TERT Antibody (2C4) [NB100-317] - Normal human pancreas showing moderate staining of exocrine cells and a subset of islets of Langerhans.

Flow Cytometry: TERT Antibody (2C4) [NB100-317] - An intracellular stain was performed on Jurkat cells with TERT Antibody (2C4) NB100-317 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse IgM Alexa Fluor 488-conjugated secondary antibody.

Co-localization of HIF-2 alpha with HIF-1 beta and RNAPII. (a) Immunofluorescence detecting indicated proteins using antibodies labelled with Alexa Fluor 555 (red, pseudocolour) and Alexa Fluor 488 (green, pseudocolour). ‘Merge’ is the red image superimposed onto the green image of the co-stained nuclear proteins. ‘Coloc’ is the co-localization channel calculated using ImageJ plugin Co-localization Threshold. White indicates pixels where both red and green signal is found (i.e. co-localization). ‘Overlay’ is the co-localization image superimposed onto the merged image. Inlay is the magnified region (white square). White arrows highlight regions of co-localization. Scale bar, 5 μm. Abbreviations: RNAPII, RNA Polymerase II phospho-serine 5. (b) Immunofluorescence images were analysed using ImageJ plugin Co-localization Threshold with use of the Costes et al. [31] method to automatically create a threshold prior to calculating the Mander's coefficient for both proteins. The results are given as, for example, the percentage of protein A (HIF-2 alpha ) that co-localized with protein B (HIF-1 beta or RNAPII) and vice versa. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/27655733), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

ATM is activated by spermidine in GM00637 cells.GM00637 cells with or without KU55933 pretreatment (a,b), and GM05849 cells (c) were exposed to 50 μM spermidine or CCCP, followed by immunofluorescence analyses of total and p-ATM on Ser-1981. The scale bar is 10 μm. Ratios of cells expressing p-ATM Ser-1981 to cells expressing total ATM were presented (d). 20–45 cells/condition from three experiments were collected. Values are mean ± SD, *p 0.05. Shown are the mean ±SEM for three independent experiments. Image collected and cropped by CiteAb from the following open publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.11023), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

MiR‐200c inhibition promoted cardiac hypertrophy via regulation of apoptosis. (A,B) Cell apoptosis was detected by flow cytometric analysis, and the percentage of apoptotic cells transfected with miR‐200c mimics or NC mimics and treated with AngII or phosphate‐buffered saline (PBS) for 48 h was evaluated; (C,D) The protein expression levels of Bcl‐2, Bax and cleaved caspase‐3 were detected in the different groups using Western blotting and were normalized to the housekeeping gene GAPDH; (E,F) Cell apoptosis was detected by flow cytometric analysis, and the percentage of apoptotic cells transfected with miR‐200c inhibitor or NC inhibitor and treated with AngII or PBS for 48 h was determined; (G,H) The protein expression levels of Bcl‐2, Bax and cleaved caspase‐3 were detected in the different groups using Western blotting and were normalized to the housekeeping gene GAPDH. (All data are presented as the means ± SD; n = 4 samples per experimental group; *P < 0.05 vs NC mimics or NC inhibitor/PBS; #P < 0.05 vs NC mimics or NC inhibitor/AngII) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30680929), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-80975

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NB100-297

TERT Antibody (2D8)

(9 Publications)

0.1 ml

NB100-297

Western Blot: TERT Antibody (2D8) [NB100-297] - Analysis of human TERT, using NB100-297. Sample: MJ90 whole cell lysate.

Immunocytochemistry/Immunofluorescence: TERT Antibody (2D8) [NB100-297] - Detection of Tert (green) in HepG2 cells using NB100-297. Nuclei (Blue) are counterstained with Hoechst 33258.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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