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6 Results for "novus"

NBP1-89341

RFC2 Antibody

(1 Publication)

0.1 ml

NBP1-89341

Western Blot: RFC2 Antibody [NBP1-89341] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).

Immunocytochemistry/Immunofluorescence: RFC2 Antibody [NBP1-89341] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

Immunohistochemistry-Paraffin: RFC2 Antibody [NBP1-89341] - Staining of human vulva/anal skin shows strong nuclear positivity in squamous epithelial cells.

Western Blot: RFC2 Antibody [NBP1-89341] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp

Immunohistochemistry: RFC2 Antibody [NBP1-89341] - RFC2 expression correlated with clinicopathological features in our LGG samples. The immunohistochemistry images showing the relative expression of RFC2 in LGG tissues and benign brain tumor tissues (Original magnification, x 400). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35210438/) licensed under a CC-BY license.

$Increased mitochondrial localization of CREB under hypoxiaA. The distribution of CREB in different cell fractions was analysed by using a commercial kit for separations followed by Western blot analysis of the distinct fractions. Anti-MEK1 (cytosol) and anti-histone H3 (nucleus) mAb served as markers for the subcellular compartments. “W” marks the whole cell lysate, “C” the cytosolic fraction, “M” the membrane/mitochondrial fraction and “N” the nuclear fraction. The picture represents one of three independent experiments. B. Cells were incubated for 24 h under hypoxia or normoxia and 30 min before terminating the cells were stained with 500 nM MitoTracker. After removing the media and fixation with 4% PFA for 20 min the cells were permeabilized with 0.5% Triton X100 in HBBS (HBSS-T) for 30 min. Following incubation with CREB antibody overnight at 4°C the cells were washed three times with HBSS, incubated with the secondary antibody (rabbit-Alexa 488) for 1 h, washed again three times with HBSS and then the nuclei were stained with DAPI. Single and merged colors recorded at a Pathway 855 (BD) are shown. Note the complete loss of nuclear CREB in dividing cells (white arrow heads). The bar represents 100 μm; Magnification: 20x. Morphology of the cells cultivated under normoxia or hypoxia (24 h each) was recorded by microscopic pictures. The pictures were taken on living, non-stained cells (transmitted). C. 1 mg isolated intact mitochondria were incubated in 1% Triton X100 and/or 10 U proteinase K in proteinase K buffer and were incubated at 37°C for 30 min and 60°C for 10 min. The reaction was stopped by boiling the probes in Laemmli buffer for 5 min and the stability of CREB was analysed by Western blot using an anti-CREB-specific mAb as described in Materials and Methods. D. Mitochondria were sub fractionated into outer membrane (OM), inter membrane space (IMS), inner membrane (IM) and mitochondrial matrix (MM). The purity of the fractions was analysed with marker proteins: TOM20 for OM, AIF for IMS, COXIV for IM, PDK4 for MM. E. Cells were cultured in the presence or absence of 50 ng/ml Leptomycin B, 10 μM LY294002 and 10 μM PYR-41 for 24 h under hypoxic conditions. Then cellular proteins were fractionated as described in the Material and Methods section. Additionally to the marker proteins used in (A) AIF served as a marker for the mitochondrial fraction. “W” represents the whole cell lysate, “C” the cytosolic fraction, “M” the membrane/mitochondrial fraction and “N” the nuclear fraction. The picture shows one of two experiments. An accumulation of highly modified CREB and the nuclear translocation of MEK1 and partially of AIF was found under leptomycin B treatment. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/27409833), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP2-45570

RFC2 Antibody (OTI2B2)

0.1 ml

NBP2-45570

Western Blot: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC2.

Immunohistochemistry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of Adenocarcinoma of Human endometrium tissue.

Flow Cytometry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by RFC2 antibody.

Immunohistochemistry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of Human breast tissue.

Immunohistochemistry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of Human Kidney tissue.

Immunohistochemistry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of Human prostate tissue.

Immunohistochemistry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of Human pancreas tissue.

Immunohistochemistry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of Carcinoma of Human prostate tissue.

Immunohistochemistry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of Human Ovary tissue.

Immunohistochemistry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of Human lymphoma tissue.

Immunohistochemistry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of Carcinoma of Human thyroid tissue.

Immunohistochemistry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of Adenocarcinoma of Human ovary tissue.

Immunohistochemistry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of Human tonsil tissue.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Mouse monoclonal

Host: Mouse monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-73857AF350
Alexa Fluor 405	NBP2-73857AF405
Alexa Fluor 488	NBP2-73857AF488
Alexa Fluor 532	NBP2-73857AF532
Alexa Fluor 594	NBP2-73857AF594
Alexa Fluor 647	NBP2-73857AF647
Alexa Fluor 700	NBP2-73857AF700
Alexa Fluor 750	NBP2-73857AF750
Allophycocyanin	NBP2-73857APC
Biotin	NBP2-73857B
DyLight 350	NBP2-73857UV
DyLight 405	NBP2-73857V
DyLight 488	NBP2-73857G
DyLight 550	NBP2-73857R
DyLight 594	NBP2-73857DL594
DyLight 650	NBP2-73857C
DyLight 680	NBP2-73857FR
DyLight 755	NBP2-73857IR
FITC	NBP2-73857F
HRP	NBP2-73857H
Janelia Fluor 549	NBP2-73857JF549
Janelia Fluor 646	NBP2-73857JF646
PE	NBP2-73857PE
PerCP	NBP2-73857PCP
mFluor Violet 450 SE	NBP2-73857MFV450
mFluor Violet 500 SE	NBP2-73857MFV500
mFluor Violet 610 SE	NBP2-73857MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-73857

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NBP2-03934

RFC2 Antibody (OTI4C11)

0.1 ml

NBP2-03934

Western Blot: RFC2 Antibody (4C11) [NBP2-03934] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RFC2.

Immunohistochemistry-Paraffin: RFC2 Antibody (4C11) [NBP2-03934] Staining of paraffin-embedded Human tonsil using anti-RFC2 mouse monoclonal antibody.

Flow Cytometry: RFC2 Antibody (4C11) [NBP2-03934] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RFC2 antibody, and then analyzed by flow cytometry.

Immunohistochemistry-Paraffin: RFC2 Antibody (4C11) [NBP2-03934] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RFC2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: RFC2 Antibody (4C11) [NBP2-03934] - Staining of paraffin-embedded Human pancreas tissue using anti-RFC2 mouse monoclonal antibody.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-73858AF350
Alexa Fluor 405	NBP2-73858AF405
Alexa Fluor 488	NBP2-73858AF488
Alexa Fluor 532	NBP2-73858AF532
Alexa Fluor 594	NBP2-73858AF594
Alexa Fluor 647	NBP2-73858AF647
Alexa Fluor 700	NBP2-73858AF700
Alexa Fluor 750	NBP2-73858AF750
Allophycocyanin	NBP2-73858APC
Biotin	NBP2-73858B
DyLight 350	NBP2-73858UV
DyLight 405	NBP2-73858V
DyLight 488	NBP2-73858G
DyLight 550	NBP2-73858R
DyLight 594	NBP2-73858DL594
DyLight 650	NBP2-73858C
DyLight 680	NBP2-73858FR
DyLight 755	NBP2-73858IR
FITC	NBP2-73858F
HRP	NBP2-73858H
Janelia Fluor 549	NBP2-73858JF549
Janelia Fluor 646	NBP2-73858JF646
PE	NBP2-73858PE
PerCP	NBP2-73858PCP
mFluor Violet 450 SE	NBP2-73858MFV450
mFluor Violet 500 SE	NBP2-73858MFV500
mFluor Violet 610 SE	NBP2-73858MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-73858

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NBP1-32253

RFC2 Antibody

0.1 ml

NBP1-32253

Western Blot: RFC2 Antibody [NBP1-32253] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RFC2 antibody diluted by 1:1000.

Immunocytochemistry/Immunofluorescence: RFC2 Antibody [NBP1-32253] - Paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.

Western Blot: RFC2 Antibody [NBP1-32253] - A: A431 B: H1299 C: Hela 10% SDS PAGE; antibody diluted at 1:1000

Reactivity:

Hu, Mu, Rt, Bv, Ch, RM, Xp

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Ch, RM, Xp

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP2-45569

RFC2 Antibody (OTI11C6)

0.1 ml

NBP2-45569

Western Blot: RFC2 Antibody (11C6) [NBP2-45569] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC2.

Flow Cytometry: RFC2 Antibody (11C6) [NBP2-45569] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by RFC2 antibody.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Mouse monoclonal

Host: Mouse monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-73856AF350
Alexa Fluor 405	NBP2-73856AF405
Alexa Fluor 488	NBP2-73856AF488
Alexa Fluor 532	NBP2-73856AF532
Alexa Fluor 594	NBP2-73856AF594
Alexa Fluor 647	NBP2-73856AF647
Alexa Fluor 700	NBP2-73856AF700
Alexa Fluor 750	NBP2-73856AF750
Allophycocyanin	NBP2-73856APC
Biotin	NBP2-73856B
DyLight 350	NBP2-73856UV
DyLight 405	NBP2-73856V
DyLight 488	NBP2-73856G
DyLight 550	NBP2-73856R
DyLight 594	NBP2-73856DL594
DyLight 650	NBP2-73856C
DyLight 680	NBP2-73856FR
DyLight 755	NBP2-73856IR
FITC	NBP2-73856F
HRP	NBP2-73856H
Janelia Fluor 549	NBP2-73856JF549
Janelia Fluor 646	NBP2-73856JF646
PE	NBP2-73856PE
PerCP	NBP2-73856PCP
mFluor Violet 450 SE	NBP2-73856MFV450
mFluor Violet 500 SE	NBP2-73856MFV500
mFluor Violet 610 SE	NBP2-73856MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-73856

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NBP2-73857AF532

RFC2 Antibody (OTI2B2) [Alexa Fluor® 532]

0.1 ml

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Alexa Fluor 532

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-73857AF350
Alexa Fluor 405	NBP2-73857AF405
Alexa Fluor 488	NBP2-73857AF488
Alexa Fluor 594	NBP2-73857AF594
Alexa Fluor 647	NBP2-73857AF647
Alexa Fluor 700	NBP2-73857AF700
Alexa Fluor 750	NBP2-73857AF750
Allophycocyanin	NBP2-73857APC
Biotin	NBP2-73857B
DyLight 350	NBP2-73857UV
DyLight 405	NBP2-73857V
DyLight 488	NBP2-73857G
DyLight 550	NBP2-73857R
DyLight 594	NBP2-73857DL594
DyLight 650	NBP2-73857C
DyLight 680	NBP2-73857FR
DyLight 755	NBP2-73857IR
FITC	NBP2-73857F
HRP	NBP2-73857H
Janelia Fluor 549	NBP2-73857JF549
Janelia Fluor 646	NBP2-73857JF646
PE	NBP2-73857PE
PerCP	NBP2-73857PCP
mFluor Violet 450 SE	NBP2-73857MFV450
mFluor Violet 500 SE	NBP2-73857MFV500
mFluor Violet 610 SE	NBP2-73857MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-73857

compare

(select up to 3 products from this page to compare)

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