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![Western Blot: PARP Antibody (194C1439) - Cleaved [NB100-56599] - Analysis of cleaved PARP in staurosporine-treated Jurkat cells at various time points, antibody at 2 ug/mL. The band corresponding to cleaved PARP is only seen in the treated samples. Anti-mouse Ig HRP conjugate was used in this test.](http://images.novusbio.com/fullsize/PARP-Antibody-194C1439-Cleaved-Western-Blot-NB100-56599-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: PARP Antibody (194C1439) - Cleaved [NB100-56599] - Analysis of cleaved PARP in staurosporine-treated Jurkat cells at various time points, antibody at 2 ug/mL. The band corresponding to cleaved PARP is only seen in the treated samples. Anti-mouse Ig HRP conjugate was used in this test.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: PARP Antibody (194C1439) - Cleaved [NB100-56599] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-PARP Antibody (194C1439) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/PARP-Antibody-194C1439-Cleaved-Immunocytochemistry-Immunofluorescence-NB100-56599-img0005.jpg)
![Western Blot: PARP Antibody (194C1439) - Cleaved [NB100-56599] - Analysis using 293T lysates expressing PARP. Image from verified customer review.](http://images.novusbio.com/fullsize/PARP-Antibody-194C1439-Cleaved-Western-Blot-NB100-56599-img0003.jpg)
![Western Blot: PARP Antibody (CL2220) [NBP2-36747] - analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PARP1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2220-Western-Blot-NBP2-36747-img0014.jpg "<b>Genetic Strategies Validation. </b>Western Blot: PARP Antibody (CL2220) [NBP2-36747] - analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PARP1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: PARP Antibody (CL2220) [NBP2-36747] - Staining in U251 cell line showing cell cycle dependent nuclear staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2220-Immunocytochemistry-Immunofluorescence-NBP2-36747-img0012.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody (CL2220) [NBP2-36747] - Staining of human small intestine shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2220-Immunohistochemistry-Paraffin-NBP2-36747-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: PARP Antibody (CL2220) [NBP2-36747] - Staining in HeLa cell line showing cell cycle dependent nuclear staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2220-Immunocytochemistry-Immunofluorescence-NBP2-36747-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: PARP Antibody (CL2220) [NBP2-36747] - Staining of human cell line A431 showing cell cycle dependent nuclear staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2220-Immunocytochemistry-Immunofluorescence-NBP2-36747-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: PARP Antibody (CL2220) [NBP2-36747] - Staining in MCF7 cell line showing clear cycle dependent nuclear staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2220-Immunocytochemistry-Immunofluorescence-NBP2-36747-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: PARP Antibody (CL2220) [NBP2-36747] - Staining in U2OS cell line with Anti-PARP1 monoclonal antibody, showing cell cycle dependent nuclear staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2220-Immunocytochemistry-Immunofluorescence-NBP2-36747-img0011.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody (CL2220) [NBP2-36747] - Staining of human tonsil shows strong nuclear positivity in lymphoid cells.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2220-Immunohistochemistry-Paraffin-NBP2-36747-img0015.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody (CL2220) [NBP2-36747] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2220-Immunohistochemistry-Paraffin-NBP2-36747-img0016.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody (CL2220) [NBP2-36747] - Staining of human Fallopian tube shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2220-Immunohistochemistry-Paraffin-NBP2-36747-img0017.jpg)