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![Western Blot: Leptin R Antibody (JA73-01) [NBP2-66931] - Analysis of Leptin Receptor on HepG2 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Leptin-R-Antibody-JA73-01-Western-Blot-NBP2-66931-img0010.jpg "Western Blot: Leptin R Antibody (JA73-01) [NBP2-66931] - Analysis of Leptin Receptor on HepG2 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Leptin R Antibody (JA73-01) [NBP2-66931] - Staining Leptin Receptor in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Leptin-R-Antibody-JA73-01-Immunocytochemistry-Immunofluorescence-NBP2-66931-img0002.jpg)
![Immunohistochemistry-Paraffin: Leptin R Antibody (JA73-01) [NBP2-66931] - Analysis of paraffin-embedded rat heart tissue using anti-Leptin Receptor antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Leptin-R-Antibody-JA73-01-Immunohistochemistry-Paraffin-NBP2-66931-img0007.jpg)
![Flow Cytometry: Leptin R Antibody (JA73-01) [NBP2-66931] - Analysis of K562 cells with Leptin Receptor antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/Leptin-R-Antibody-JA73-01-Flow-Cytometry-NBP2-66931-img0001.jpg)
![Western Blot: Leptin R Antibody (JA73-01) [NBP2-66931] - Western blot analysis of Leptin R on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse lung tissue lysate Lane 2: mouse liver tissue lysate](http://images.novusbio.com/fullsize/Leptin-R-Antibody-JA73-01-Western-Blot-NBP2-66931-img0009.jpg)
![Immunohistochemistry-Paraffin: Leptin R Antibody (JA73-01) [NBP2-66931] - Analysis of paraffin-embedded human kidney tissue using anti-Leptin Receptor antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Leptin-R-Antibody-JA73-01-Immunohistochemistry-Paraffin-NBP2-66931-img0003.jpg)
![Immunohistochemistry-Paraffin: Leptin R Antibody (JA73-01) [NBP2-66931] - Analysis of paraffin-embedded human liver cancer tissue using anti-Leptin Receptor antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Leptin-R-Antibody-JA73-01-Immunohistochemistry-Paraffin-NBP2-66931-img0004.jpg)
![Immunohistochemistry-Paraffin: Leptin R Antibody (JA73-01) [NBP2-66931] - Analysis of paraffin-embedded human prostate tissue using anti-Leptin Receptor antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Leptin-R-Antibody-JA73-01-Immunohistochemistry-Paraffin-NBP2-66931-img0005.jpg)
![Immunohistochemistry-Paraffin: Leptin R Antibody (JA73-01) [NBP2-66931] - Analysis of paraffin-embedded mouse small intestine tissue using anti-Leptin Receptor antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Leptin-R-Antibody-JA73-01-Immunohistochemistry-Paraffin-NBP2-66931-img0006.jpg)
![Western Blot: Leptin R Antibody [NB100-40796] - (1.75ug/ml) staining of Human Cerebellum (A), and (1.5ug/ml) Mouse Foetal Brain (B) and Rat Brain (C) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Leptin-R-Antibody-Western-Blot-NB100-40796-img0001.jpg "Western Blot: Leptin R Antibody [NB100-40796] - (1.75ug/ml) staining of Human Cerebellum (A), and (1.5ug/ml) Mouse Foetal Brain (B) and Rat Brain (C) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunohistochemistry: Leptin R Antibody [NB100-40796] - (6ug/ml) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.](http://images.novusbio.com/fullsize/Leptin-R-Antibody-Immunohistochemistry-NB100-40796-img0002.jpg)
![Flow Cytometry: Leptin R Antibody [NB100-40796] - Analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Leptin-R-Antibody-Flow-Cytometry-NB100-40796-img0004.jpg)
![Control: Leptin R Antibody [NB100-40796] - Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.](http://images.novusbio.com/fullsize/Leptin-R-Antibody-Control-NB100-40796-img0003.jpg)
![Western Blot: Leptin R Antibody [NB120-5593] - Analysis of both short and long forms of OB-R from mouse lung tissue extract.](http://images.novusbio.com/fullsize/Leptin-R-Antibody-Western-Blot-NB120-5593-img0005.jpg "Western Blot: Leptin R Antibody [NB120-5593] - Analysis of both short and long forms of OB-R from mouse lung tissue extract.")
![Immunocytochemistry/Immunofluorescence: Leptin R Antibody [NBP1-85765] - Staining of human cell line skin taken from normal foreskin shows no positivity.](http://images.novusbio.com/fullsize/Leptin-R-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85765-img0007.jpg "Immunocytochemistry/Immunofluorescence: Leptin R Antibody [NBP1-85765] - Staining of human cell line skin taken from normal foreskin shows no positivity.")
![Immunohistochemistry-Paraffin: Leptin R Antibody [NBP1-85765] - Staining of human choroid plexus shows strong membranous positivity in the ependymal cells.](http://images.novusbio.com/fullsize/Leptin-R-Antibody-Immunohistochemistry-Paraffin-NBP1-85765-img0006.jpg)
![Western Blot: Leptin R Antibody (RM0085-9C29) - Azide and BSA Free [NBP1-21725] - Lysates of mouse embryonic tissue with rat anti-mouse Leptin R antibody.](http://images.novusbio.com/fullsize/Leptin-R-Antibody-RM0085-9C29-Azide-and-BSA-Free-Western-Blot-NBP1-21725-img0001.jpg "Western Blot: Leptin R Antibody (RM0085-9C29) - Azide and BSA Free [NBP1-21725] - Lysates of mouse embryonic tissue with rat anti-mouse Leptin R antibody.")
![Western Blot: Leptin Receptor Overexpression Lysate (Adult Normal) [NBP2-10740] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Leptin Receptor.](http://images.novusbio.com/fullsize/Leptin-Receptor-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10740-img0001.jpg "Western Blot: Leptin Receptor Overexpression Lysate (Adult Normal) [NBP2-10740] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Leptin Receptor.")
![Western Blot: Leptin Receptor Overexpression Lysate (Adult Normal) [NBL1-12490] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Leptin Receptor.](http://images.novusbio.com/fullsize/Leptin-Receptor-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12490-img0002.jpg "Western Blot: Leptin Receptor Overexpression Lysate (Adult Normal) [NBL1-12490] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Leptin Receptor.")
![ELISA: Mouse Leptin R ELISA Kit (Colorimetric) [NBP3-11805] - Samples were spiked with high concentrations of Mouse Leptin R and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-11805_mouse-leptin-r-elisa-kit-colorimetric-132202416143034.jpg "ELISA: Mouse Leptin R ELISA Kit (Colorimetric) [NBP3-11805] - Samples were spiked with high concentrations of Mouse Leptin R and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Leptin R ELISA Kit (Colorimetric) [NBP3-11805] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-11805_mouse-leptin-r-elisa-kit-colorimetric-4320249151667.jpg)
![Sandwich ELISA: Leptin R Antibody (2H5) [H00003953-M05] - Detection limit for recombinant GST tagged LEPR is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Leptin-R-Antibody-2H5-Sandwich-ELISA-H00003953-M05-img0001.jpg "Sandwich ELISA: Leptin R Antibody (2H5) [H00003953-M05] - Detection limit for recombinant GST tagged LEPR is 1 ng/ml as a capture antibody.")