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![Immunohistochemistry-Paraffin: CCR7 Antibody [NB100-712] - M1 and M2 macrophages showed similar expression patterns in cortical bone. IHC staining was performed in sagittal serial sections of medial condyles from 6-week-old rats within the diaphyseal region. Cluster of differentiation 68 (CD68) was selected as a pan marker for the macrophage lineage, including monocytes, macrophages, giant cells, and osteoclasts. iNOS and C-C chemokine receptor type 7 (CCR7) were selected as M1 phenotypic markers, whereas Arginase1 and cluster of differentiation 163 (CD163) were selected as M2 phenotypic markers. Tartrate-resistant acid phosphatase (TRAP) staining was performed as described in Materials and Methods. Quantification of cell numbers according to their location revealed that most of the M1 and M2 cells were not attached to the bone surfaces. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/boneres201719) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CCR7-Antibody-Immunohistochemistry-Paraffin-NB100-712-img0006.jpg "Immunohistochemistry-Paraffin: CCR7 Antibody [NB100-712] - M1 and M2 macrophages showed similar expression patterns in cortical bone. IHC staining was performed in sagittal serial sections of medial condyles from 6-week-old rats within the diaphyseal region. Cluster of differentiation 68 (CD68) was selected as a pan marker for the macrophage lineage, including monocytes, macrophages, giant cells, and osteoclasts. iNOS and C-C chemokine receptor type 7 (CCR7) were selected as M1 phenotypic markers, whereas Arginase1 and cluster of differentiation 163 (CD163) were selected as M2 phenotypic markers. Tartrate-resistant acid phosphatase (TRAP) staining was performed as described in Materials and Methods. Quantification of cell numbers according to their location revealed that most of the M1 and M2 cells were not attached to the bone surfaces. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/boneres201719) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: CCR7 Antibody [NB100-712] - Mouse spleen](http://images.novusbio.com/fullsize/CCR7-Antibody-Immunohistochemistry-Paraffin-NB100-712-img0001.jpg)
![Immunohistochemistry-Paraffin: CCR7 Antibody [NB100-712] - Mouse spleen](http://images.novusbio.com/fullsize/CCR7-Antibody-Immunohistochemistry-Paraffin-NB100-712-img0004.jpg)
![Immunohistochemistry-Paraffin: CCR7 Antibody [NB100-712] - Human spleen](http://images.novusbio.com/fullsize/CCR7-Antibody-Immunohistochemistry-Paraffin-NB100-712-img0005.jpg)
![Western Blot: CCR7 Antibody (SR36-04) [NBP2-67324] - Analysis of CCR7 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysateLane 2: MCF-7 cell lysate Lane 3: PC-12 cell lysate Lane 4: Daudi cell lysate](http://images.novusbio.com/fullsize/CCR7-Antibody-SR36-04-Western-Blot-NBP2-67324-img0009.jpg "Western Blot: CCR7 Antibody (SR36-04) [NBP2-67324] - Analysis of CCR7 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysateLane 2: MCF-7 cell lysate Lane 3: PC-12 cell lysate Lane 4: Daudi cell lysate")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CCR7 Antibody (SR36-04) [NBP2-67324] - Gross and histological appearance of lung excised from 13 weeks of age PyMT transgenic females. CRE treated PyMT transgenic females showed decreased frequency of mammary tumor-derived lung metastases. Immunohistochemistry staining against Ccr7 in lung tissues was done and representative images of tissue sections from each group were taken at 200X and 40X magnification. Scale bars = 50um. Histogram data was presented as mean +/- SEM. * p < 0.05 vs. control. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6643/11/2/410), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CCR7-Antibody-SR36-04-Immunocytochemistry-Immunofluorescence-NBP2-67324-img0006.jpg)
![Immunohistochemistry-Paraffin: CCR7 Antibody (SR36-04) [NBP2-67324] - Analysis of paraffin-embedded human tonsil tissue using anti-CCR7 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CCR7-Antibody-SR36-04-Immunohistochemistry-Paraffin-NBP2-67324-img0004.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: CCR7 Antibody (SR36-04) [NBP2-67324] - Gross and histological appearance of lung excised from 13 weeks of age PyMT transgenic females. CRE treated PyMT transgenic females showed decreased frequency of mammary tumor-derived lung metastases. Level of Ccr7 protein in lung tissues was analyzed by Western blot. Gapdh was used as an internal control. * p < 0.05 vs. control. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6643/11/2/410), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CCR7-Antibody-SR36-04-Western-Blot-NBP2-67324-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CCR7 Antibody (SR36-04) [NBP2-67324] - Staining CCR7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CCR7-Antibody-SR36-04-Immunocytochemistry-Immunofluorescence-NBP2-67324-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CCR7 Antibody (SR36-04) [NBP2-67324] - Staining CCR7 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CCR7-Antibody-SR36-04-Immunocytochemistry-Immunofluorescence-NBP2-67324-img0002.jpg)
![Immunohistochemistry-Paraffin: CCR7 Antibody (SR36-04) [NBP2-67324] - Analysis of paraffin-embedded human spleen tissue using anti-CCR7 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CCR7-Antibody-SR36-04-Immunohistochemistry-Paraffin-NBP2-67324-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: CCR7 Antibody (SR36-04) [NBP2-67324] - Downregulation of CCR7 gene by siRNA inhibits gene expression of MMP9, c-FOS, c-JUN and CCL21 in vitro. Western blot of proteins extracted from MCF7 cell against CCR7 and GAPDH. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6643/11/2/410), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CCR7-Antibody-SR36-04-Knockdown-Validated-NBP2-67324-img0007.jpg)
![Immunohistochemistry-Paraffin: CCR7 Antibody [NLS848] - Analysis of anti-CCR7 antibody with transfected cells expressing CCR7 at 14 ug/ ml.](http://images.novusbio.com/fullsize/CCR7-Antibody-Immunohistochemistry-Paraffin-NLS848-img0008.jpg "Immunohistochemistry-Paraffin: CCR7 Antibody [NLS848] - Analysis of anti-CCR7 antibody with transfected cells expressing CCR7 at 14 ug/ ml.")
![Immunohistochemistry-Paraffin: CCR7 Antibody [NLS848] - Analysis of anti-CCR7 antibody with transfection control at 14 ug/ml.](http://images.novusbio.com/fullsize/CCR7-Antibody-Immunohistochemistry-Paraffin-NLS848-img0002.jpg)
![Immunohistochemistry-Paraffin: CCR7 Antibody [NLS848] - Analysis of anti-CCR7 antibody with human tonsil at dilution: 14 ug/ml](http://images.novusbio.com/fullsize/CCR7-Antibody-Immunohistochemistry-Paraffin-NLS848-img0007.jpg)
![Immunohistochemistry-Paraffin: CCR7 Antibody [NLS848] - Analysis of anti-CCR7 antibody with human spleen at dilution 14 ug/ml.](http://images.novusbio.com/fullsize/CCR7-Antibody-Immunohistochemistry-Paraffin-NLS848-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: CCR7 Antibody (4B12) - Chimeric [NBP3-12012] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 4B12 (NBP3-12012) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12012, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/CCR7-Antibody-4B12-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12012-img0001.jpg "Immunocytochemistry/Immunofluorescence: CCR7 Antibody (4B12) - Chimeric [NBP3-12012] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 4B12 (NBP3-12012) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12012, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
