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![Knockout Validated: TBK1 Antibody (108A429) [NB100-56705] - Western blot using lysates from U20S parental cell line and TBK1 knockout U20S cell line (KO), collected in RIPA buffer. Nitrocellulose membrane was probed with Mouse Anti-Human/Mouse/Rat TBK1 Monoclonal Antibody (Catalog # NB100-56705) at 1 ug/ul O/N at 4C, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody and ECL detection. A specific band at 83 kDa was detected for TBK1 in the parental U20S cell line, but is not detectable in knockout U20S cell line. The Ponceau stained transfers of each blot are shown to confirm equal protein loading. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/TBK1-Antibody-108A429-Knockout-Validated-NB100-56705-img0023.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: TBK1 Antibody (108A429) [NB100-56705] - Western blot using lysates from U20S parental cell line and TBK1 knockout U20S cell line (KO), collected in RIPA buffer. Nitrocellulose membrane was probed with Mouse Anti-Human/Mouse/Rat TBK1 Monoclonal Antibody (Catalog # NB100-56705) at 1 ug/ul O/N at 4C, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody and ECL detection. A specific band at 83 kDa was detected for TBK1 in the parental U20S cell line, but is not detectable in knockout U20S cell line. The Ponceau stained transfers of each blot are shown to confirm equal protein loading. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).")
![<b>Genetic Strategies Validation. </b>Simple Western: TBK1 Antibody (108A429) [NB100-56705] - Lane view shows a specific band for NAK/TBK1 in 0.5 mg/ml of Daudi (left) and Raw 264.7 (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody](http://images.novusbio.com/fullsize/TBK1-Antibody-108A429-Simple-Western-NB100-56705-img0014.jpg)
![Immunohistochemistry-Paraffin: TBK1 Antibody (108A429) [NB100-56705] - Analysis of FFPE human testis tissue section using Peripheral Node Addressin antibody (clone MECA-79R) at 1:200. The staining was developed with HRP-DAB detection method and the counterstaining was performed using hematoxylin.](http://images.novusbio.com/fullsize/TBK1-Antibody-108A429-Immunohistochemistry-Paraffin-NB100-56705-img0021.jpg)
![Western Blot: TBK1 Antibody (108A429) [NB100-56705] - Analysis using Azide and BSA Free version of NB100-56705. Lysate from 293 cells untransfected (lane 1) and transfected with human TBK1 cDNA (lane 2) probed with TBK1 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/TBK1-Antibody-108A429-Western-Blot-NB100-56705-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: TBK1 Antibody (108A429) [NB100-56705] - Antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). A dilution of 1:10 was used. Image objective 40x.](http://images.novusbio.com/fullsize/TBK1-Antibody-108A429-Immunocytochemistry-Immunofluorescence-NB100-56705-img0013.jpg)
![Immunohistochemistry-Paraffin: TBK1 Antibody (108A429) [NB100-56705] - Immunohistochemistry: TBK1 Antibody (108A429) - Azide and BSA Free [NBP2-33243] - Staining on human testis (Formalin-Fixed Paraffin-Embedded (FFPE)) Image from the Azide and BSA Free version of this antibody.](http://images.novusbio.com/fullsize/TBK1-Antibody-108A429-Immunohistochemistry-Paraffin-NB100-56705-img0022.jpg)
![Western Blot: TBK1 Antibody (108A429) [NB100-56705] - Analysis of Human Daudi (A) and mouse RAW (B) cell lysate probed with TBK1 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/TBK1-Antibody-108A429-Western-Blot-NB100-56705-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: TBK1 Antibody (108A429) [NB100-56705] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-TBK1 at 20 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/TBK1-Antibody-108A429-Immunocytochemistry-Immunofluorescence-NB100-56705-img0019.jpg)
![Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Brain Cerebellum Purkinje Neuron](http://images.novusbio.com/fullsize/CXCR4-Antibody-Immunohistochemistry-Paraffin-NLS1380-img0010.jpg "Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Brain Cerebellum Purkinje Neuron")
![Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Analysis of anti-CXCR4 antibody with human tonsil.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Immunohistochemistry-Paraffin-NLS1380-img0004.jpg)
![Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Analysis of anti-CXCR4 antibody with human colon, carcinoma.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Immunohistochemistry-Paraffin-NLS1380-img0006.jpg)
![Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Human Lymph Node, Non-Hodgkins Lymphoma formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Immunohistochemistry-Paraffin-NLS1380-img0007.jpg)
![Immunohistochemistry-Frozen: CXCR4 Antibody [NLS1380] - Rat brain tissue sections. Rat Nucleus Accumbens treated with MDPV. Images taken with fluorescent microscope. Primary antibody was diluted to 1:200. Secondary antibody used was donkey anti-Rabbit Alexa Fluor 594 at 1:500. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Immunohistochemistry-Frozen-NLS1380-img0008.jpg)
![Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Lung, small cell carcinoma](http://images.novusbio.com/fullsize/CXCR4-Antibody-Immunohistochemistry-Paraffin-NLS1380-img0009.jpg)
![Immunohistochemistry-Paraffin: PTGER4/EP4 Antibody [NLS3890] - Colon, Carcinoma](http://images.novusbio.com/fullsize/PTGER4-EP4-Antibody-Immunohistochemistry-Paraffin-NLS3890-img0010.jpg "Immunohistochemistry-Paraffin: PTGER4/EP4 Antibody [NLS3890] - Colon, Carcinoma")
![Immunohistochemistry-Paraffin: PTGER4/EP4 Antibody [NLS3890] - Analysis of anti-PTGER4 / EP4 antibody with human colon, ganglion at 2 ug/ml.](http://images.novusbio.com/fullsize/PTGER4-EP4-Antibody-Immunohistochemistry-Paraffin-NLS3890-img0003.jpg)
![Immunohistochemistry-Paraffin: PTGER4/EP4 Antibody [NLS3890] - Human Lung, Adenocarcinoma tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/PTGER4-EP4-Antibody-Immunohistochemistry-Paraffin-NLS3890-img0004.jpg)
![Immunohistochemistry-Paraffin: PTGER4/EP4 Antibody [NLS3890] - Human Prostate, Carcinoma tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/PTGER4-EP4-Antibody-Immunohistochemistry-Paraffin-NLS3890-img0005.jpg)
![Immunohistochemistry-Paraffin: PTGER4/EP4 Antibody [NLS3890] - Human prostate, benign prostatic hyperplasia tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/PTGER4-EP4-Antibody-Immunohistochemistry-Paraffin-NLS3890-img0007.jpg)
![Immunohistochemistry-Paraffin: PTGER4/EP4 Antibody [NLS3890] - Human Pancreas, Carcinoma tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/PTGER4-EP4-Antibody-Immunohistochemistry-Paraffin-NLS3890-img0006.jpg)
![Immunohistochemistry-Paraffin: PTGER4/EP4 Antibody [NLS3890] - Breast, Carcinoma](http://images.novusbio.com/fullsize/PTGER4-EP4-Antibody-Immunohistochemistry-Paraffin-NLS3890-img0008.jpg)
![Immunohistochemistry-Paraffin: PTGER4/EP4 Antibody [NLS3890] - Brain, Glioblastoma](http://images.novusbio.com/fullsize/PTGER4-EP4-Antibody-Immunohistochemistry-Paraffin-NLS3890-img0009.jpg)
![Western Blot: Frizzled-6 Antibody [NBP3-18335] - WB Validation in Human, Mouse and Rat Cell Lines. Loading: 15 ug of lysate. Antibodies: Frizzled-6 NBP3-18335, 2 ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.](http://images.novusbio.com/fullsize/Frizzled-6-Antibody-Western-Blot-NBP3-18335-img0001.jpg "Western Blot: Frizzled-6 Antibody [NBP3-18335] - WB Validation in Human, Mouse and Rat Cell Lines. Loading: 15 ug of lysate. Antibodies: Frizzled-6 NBP3-18335, 2 ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.")
![Immunocytochemistry/Immunofluorescence: Frizzled-6 Antibody [NBP3-18335] - Immunofluorescent analysis of 4% paraformaldehyde-fixed rat lung labeling Frizzled-6 with NBP3-18335 at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/Frizzled-6-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-18335-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Frizzled-6 Antibody [NBP3-18335] - Immunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling Frizzled-6 with NBP3-18335 at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/Frizzled-6-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-18335-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Frizzled-6 Antibody [NBP3-18335] - Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse lung labeling Frizzled-6 with NBP3-18335 at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/Frizzled-6-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-18335-img0003.jpg)
![Immunohistochemistry-Paraffin: PTGER4/EP4 Antibody [NLS3896] - Analysis of anti-PTGER4 antibody with human, colon.](http://images.novusbio.com/fullsize/PTGER4-EP4-Antibody-Immunohistochemistry-Paraffin-NLS3896-img0004.jpg "Immunohistochemistry-Paraffin: PTGER4/EP4 Antibody [NLS3896] - Analysis of anti-PTGER4 antibody with human, colon.")
![Immunohistochemistry: PTGER4/EP4 Antibody [NLS3896] - Analysis of anti-PTGER4 antibody with transfection control at 4 ug/ml.](http://images.novusbio.com/fullsize/PTGER4-EP4-Antibody-Immunohistochemistry-NLS3896-img0003.jpg)
![Immunohistochemistry: PTGER4/EP4 Antibody [NLS3896] - Analysis of anti-PTGER4 antibody with transfected cells expressing Prostaglandin E Receptor EP4 at 4 ug/ml.](http://images.novusbio.com/fullsize/PTGER4-EP4-Antibody-Immunohistochemistry-NLS3896-img0002.jpg)
![Immunohistochemistry-Paraffin: MCHR1 Antibody [NLS1533] - Anti-MCHR1 antibody IHC of human Colon, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/MCHR1-Antibody-Immunohistochemistry-Paraffin-NLS1533-img0004.jpg "Immunohistochemistry-Paraffin: MCHR1 Antibody [NLS1533] - Anti-MCHR1 antibody IHC of human Colon, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: MCHR1 Antibody [NLS1533] - Analysis of anti-MCHR1 antibody with human skin.](http://images.novusbio.com/fullsize/MCHR1-Antibody-Immunohistochemistry-Paraffin-NLS1533-img0001.jpg)
![Immunohistochemistry-Paraffin: MCHR1 Antibody [NLS1533] - Analysis of anti-MCHR1 antibody with human brain at 3 ug/ml.](http://images.novusbio.com/fullsize/MCHR1-Antibody-Immunohistochemistry-Paraffin-NLS1533-img0003.jpg)
![Immunohistochemistry-Paraffin: MCHR1 Antibody [NLS1533] - Anti-MCHR1 antibody IHC of human Soft Tissue, Liposarcoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/MCHR1-Antibody-Immunohistochemistry-Paraffin-NLS1533-img0005.jpg)
![Immunohistochemistry-Paraffin: mu Opioid R/OPRM1 Antibody [NLS234] - Brain, Neurons](http://images.novusbio.com/fullsize/mu-Opioid-R-OPRM1-Antibody-Immunohistochemistry-Paraffin-NLS234-img0001.jpg "Immunohistochemistry-Paraffin: mu Opioid R/OPRM1 Antibody [NLS234] - Brain, Neurons")
![Immunohistochemistry-Paraffin: MCHR1 Antibody [NLS1532] - Analysis of anti-SLC1 / MCHR1 antibody with human adrenal at dilution 15-30 ug/ml.](http://images.novusbio.com/fullsize/MCHR1-Antibody-Immunohistochemistry-Paraffin-NLS1532-img0001.jpg "Immunohistochemistry-Paraffin: MCHR1 Antibody [NLS1532] - Analysis of anti-SLC1 / MCHR1 antibody with human adrenal at dilution 15-30 ug/ml.")