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![Western Blot: UBR5/EDD Antibody [NB100-1591] - Samples: Whole cell lysate (50 ug) from HEK293T, Jurkat, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit UBR5/EDD Antibody (NB100-1591, lot 6) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/UBR5-EDD-Antibody-Western-Blot-NB100-1591-img0003.jpg "Western Blot: UBR5/EDD Antibody [NB100-1591] - Samples: Whole cell lysate (50 ug) from HEK293T, Jurkat, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit UBR5/EDD Antibody (NB100-1591, lot 6) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: UBR5/EDD Antibody [NB100-1591] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit UBR5/EDD Antibody (NB100-1591, lot 6) used at a dilution of 1:1,000 (1 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/UBR5-EDD-Antibody-Immunohistochemistry-Paraffin-NB100-1591-img0002.jpg)
![Immunohistochemistry-Paraffin: UBR5/EDD Antibody [NB100-1591] - Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit UBR5/EDD Antibody (NB100-1591, lot 6) used at a dilution of 1:1000 (1 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/UBR5-EDD-Antibody-Immunohistochemistry-Paraffin-NB100-1591-img0001.jpg)
![Immunoprecipitation: UBR5/EDD Antibody [NB100-1591] - Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit UBR5/EDD Antibody (NB100-1591, lot 6) used for IP at 6 ug per reaction. UBR5/EDD was also immunoprecipitated by a previous lot of this antibody (lot 5). For blotting immunoprecipitated UBR5/EDD, NB100-1591 was used at 0.1 ug/ml. Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/UBR5-EDD-Antibody-Immunoprecipitation-NB100-1591-img0004.jpg)
![Immunohistochemistry-Paraffin: UBR5/EDD Antibody [NB100-1220] - Staining of paraffin embedded Human Testis. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. These results could not be obtained after antigen retrieval at pH6.](http://images.novusbio.com/fullsize/UBR5-EDD-Antibody-Immunohistochemistry-Paraffin-NB100-1220-img0002.jpg "Immunohistochemistry-Paraffin: UBR5/EDD Antibody [NB100-1220] - Staining of paraffin embedded Human Testis. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. These results could not be obtained after antigen retrieval at pH6.")
![Western Blot: UBR5/EDD Antibody [NBP3-12948] - U87-MG whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with UBR5/EDD antibody (NBP3-12948) diluted at 1:500.](http://images.novusbio.com/fullsize/UBR5-EDD-Antibody-Western-Blot-NBP3-12948-img0001.jpg "Western Blot: UBR5/EDD Antibody [NBP3-12948] - U87-MG whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with UBR5/EDD antibody (NBP3-12948) diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: UBR5/EDD Antibody [NBP3-12948] - UBR5/EDD antibody detects UBR5/EDD protein at nucleus by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: UBR5/EDD protein stained by UBR5/EDD antibody (NBP3-12948) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/UBR5-EDD-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-12948-img0002.jpg)
![UBR5/EDD Antibody [NBP1-71802] - Detection of human and mouse EDD1 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (H; 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (5, 15, and 50 ug for WB) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-EDD1 antibody NBP1-71802 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. EDD1 was less efficiently immunoprecipitated by another rabbit anti-EDD1 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/UBR5-EDD-Antibody-NBP1-71802-img0003.jpg "UBR5/EDD Antibody [NBP1-71802] - Detection of human and mouse EDD1 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (H; 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (5, 15, and 50 ug for WB) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-EDD1 antibody NBP1-71802 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. EDD1 was less efficiently immunoprecipitated by another rabbit anti-EDD1 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).")
![Western Blot: UBR5/EDD Antibody (SR1501) [NBP3-22217] - Western blot analysis using NBP3-22217 in SH-SY5Y cell lysate.](http://images.novusbio.com/fullsize/nbp3-22217_rabbit-ubr5-edd-mab-sr1501-5720231624740.jpg "Western Blot: UBR5/EDD Antibody (SR1501) [NBP3-22217] - Western blot analysis using NBP3-22217 in SH-SY5Y cell lysate.")
![Immunocytochemistry/Immunofluorescence: UBR5/EDD Antibody [NBP2-58579] - Staining of human cell line MCF7 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/UBR5-EDD-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58579-img0001.jpg "Immunocytochemistry/Immunofluorescence: UBR5/EDD Antibody [NBP2-58579] - Staining of human cell line MCF7 shows localization to nucleoplasm.")